Browsing by Author "Saglamtas, Ruya"

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Anti-Alzheimer, Antiglaucoma, and Antidiabetic Activities of Sainfoin (Onobrychis Buhseana Boiss.)
(Wiley-v C H verlag Gmbh, 2025) Jafarova, Shahla; Aydin, Tuba; Isayev, Javanshir; Saglamtas, Ruya; Ozgokce, Fevzi; Kazaz, Cavit; Cakir, Ahmet
Onobrychis Adans. species, known as 'sainfoin,' are commercially valuable plants cultivated to produce high-protein animal feed. In this study, the ethanol extract of Onobrychis buhseana Boiss., which is widely distributed in Azerbaijan, was subjected to silica gel column and thin-layer chromatography for the first time, leading to the isolation of a new natural hydroquinone oligomer, named buhseanol (1), along with two known compounds, beta-arbutin (2) and an inositol derivative (3). The chemical structure of buhseanol (1) was characterized by proton nuclear magnetic resonance (1H-NMR), carbon-13 NMR, Attached Proton Test NMR, and liquid chromatography-tandem mass spectrometry. Buhseanol (1), beta-arbutin (2), and the ethanol extract were evaluated for their anti-Alzheimer's, anti-glaucoma, and anti-diabetic effects, with both the extract and buhseanol (1) potentially showing these activities. The inositol derivative (3) did not exhibit any of these activities.
Exploring the Multipharmacological Potential of Rosa Pisiformis: An Integrated Approach Combining LC-MS/MS, GC-MS, Enzyme Inhibition, Docking, and Pathway Enrichment
(Springer, 2025) Saglamtas, Ruya; Demir, Yeliz; Kucuk, Sebahat; Ozgokce, Fevzi; Comakli, Veysel
Rosa pisiformis, an endemic rosehip species, was investigated for its phytochemical composition and therapeutic potential through an integrated LC-MS/MS, GC-MS, enzyme inhibition, molecular docking, and bioinformatic approach. LC-MS/MS identified twenty major phenolic compounds, with quinic acid (1343.87 +/- 13.17 mu g/g in methanol extract) and gallic acid (772.31 +/- 36.18 mu g/g in ethanol extract) as the dominant constituents. GC-MS profiling revealed abundant fatty acid methyl esters, notably methyl oleate and methyl linolenate. Ethanol and aqueous extracts showed potent cholinesterase inhibition (AChE EEIC50: 62.27 +/- 1.40 mu g/mL; BChE EEIC50: 19.41 +/- 0.24 mu g/mL) and tyrosinase inhibition (AEIC50: 25.63 mu g/mL), while dichloromethane and methanol extracts displayed notable alpha-glucosidase (IC50:103.11 mu g/mL) and alpha-amylase (IC50: 43.96 mu g/mL) inhibition. Principal Component Analysis (PCA) effectively discriminated between the methanol and ethanol extracts based on the dominance of quinic and gallic acids, respectively. Molecular docking confirmed strong binding affinities of quinic acid to AChE, BChE, and lipase via multiple hydrogen bonds. Bioinformatic enrichment analyses linked these compounds to detoxification, lipid metabolism, hormone biosynthesis, inflammatory regulation, and cancer-related pathways. These results provide the first systems-level evidence for the multifunctional therapeutic potential of R. pisiformis in managing neurodegenerative, metabolic, and dermatological disorders.
Isatis Glauca Aucher Ex Boiss. Subsp. Glauca (Indigo) Extracts as Enzyme Inhibitors: a Biochemical Study
(Wiley-v C H verlag Gmbh, 2025) Nezir, Beyza Sevdenur; Dasdemir, Edanur; Aydin, Tuba; Saglamtas, Ruya; Kaya, Elife; Ozgokce, Fevzi
Isatis glauca Aucher ex Boiss. subsp. glauca is a species of indigo that grows naturally in T & uuml;rkiye. In this study, the in vitro biological activities of I. glauca were evaluated for the first time. The inhibitory effects of I. glauca's methanol, ethanol, dichloromethane/methanol (50:50), acetone, ethyl acetate, dichloromethane, and n-hexane extracts were investigated on the enzymes human carbonic anhydrase I (hCAI), human carbonic anhydrase II (hCAII), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and alpha-glycosidase. IC50 values were calculated to determine the inhibitory effect of the extracts. Among the extracts, the ones with the strongest inhibitory activity against hCAI, hCAII, AChE, BChE, and alpha-glucosidase were found to be ethyl acetate extract (IC50: 75.77 mg/mL), methanol extract (IC50: 102.89 mg/mL), n-hexane extract (IC50: 61.11 mg/mL), ethyl acetate extract (IC50: 22.79 mg/mL), and ethyl acetate extract (IC50: 77.12 mg/mL), respectively. The study findings suggest that all extracts of I. glauca may have promising therapeutic properties. In particular, it can be suggested that these extracts have the potential to be used in the treatment of diseases or the alleviation of symptoms. However, additional research and clinical trials are required to determine its efficacy. The isolation of I. glauca phytochemicals should be addressed in future studies.