Browsing by Author "Sagmanligil, Vedat"

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The Effects of Moxidectin Nicotine-Conditioned Cue on Nicotine-Seeking Behavior in Mice
(inst Advanced Science Extension, 2021) Yunusoglu, Oruc; Demirkol, Muhammed Hamdi; Berkoz, Mehmet; Sagmanligil, Vedat; Oto, Gokhan; Ozdemir, Hulya
Current pharmacotherapies for nicotine abuse are few and relatively inefficient demonstrating the need for the development of new, effective remedies. Moxidectin is used as an anti-parasitic agent in both animals and humans, it also activates GABA receptors. The objective of the present investigation was to study the effect of moxidectin on nicotine-induced conditioned place preference (CPP) in male Swiss mice. Intraperitoneal (i.p.) route was used for nicotine (0.5mg/kg) administration for a 3-day conditioning program. The influences of moxidectin on the reinforcing characteristics of nicotine were tested in mice given i.p. treatment of moxidectin (5 and 10mg/kg) 30 minutes prior to per nicotine administration. CPP was extinguished by repeated testing, through which conditioned mice were daily given two doses of moxidectin (5 and 10mg/kg, i.p.). Subsequently, the potency of moxidectin in blocking the reinstatement of CPP provoked by priming given low-dose nicotine (0.1mg/kg, i.p.) was also evaluated. Moxidectin treatment illustrated a reserve of acquisition of nicotine-induced CPP. It was reduced priming nicotine-induced reinstatement and accelerated the extinction of CPP. Relatively nicotine enhanced the locomotor, motor activity but was not statistically significant. In conclusion, the outcomes demonstrate the potential for the development of moxidectin as a new pharmacotherapy for the treatment of nicotine addiction. (C) 2021 The Authors. Published by IASE.
Effects of Thymus Vulgaris L. in Acute and Chronic Epilepsy Models in Rats Induced by Pentylenetetrazole
(Kafkas Univ, veteriner Fakultesi dergisi, 2019) Ozdemir, Hulya; Sagmanligil, Vedat; Erkec, Ozlem Ergul; Oto, Gokhan; Basbugan, Yildiray; Uyar, Hasan
The aim of this study was to investigate the effects of Thymus vulgaris L. (TVL) on oxidative stress, motor coordination and learning/memory in acute and chronic epilepsy models in rats induced by Pentylenetetrazole (PTZ). To this end, 64 male Wistar-albino rats were randomly divided into eight groups with 8 rats each: (1) acute control (AC), (2) acute PTZ (APTZ), (3) acute PTZ + sodium valproate (APTZ+VPA), (4) acute PTZ + TVL (APTZ+TVL), (5) chronic control (CC), (6) PTZ kindling (PTZk), (7) PTZ kindling + VPA (PTZk+VPA) and (8) PTZ kindling + TVL (PTZk+TVL). Seizures were observed for 30 min after each PTZ injection and were scored. Acute PTZ-induced seizures were created by injecting a single convulsive dose of PTZ (60 mg/kg, ip) in acute groups. PTZ kindling was produced by injecting a subconvulsant dose of PTZ (35 mg/kg, ip) every other day, with 14 injections in total. No significant difference was found among the PTZk + VPA, PTZk, and PTZk + TVL groups with regard to seizure scores. No significant difference was found among all the 8 groups in the learning/memory tests conducted using the Morris Water Maze (MWM) test and the motor activity tests conducted using the rotarod test (P>0.05). The analysis of total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) indicated that the administration of PTZ decreased the antioxidant capacity and increased the oxidant capacity. Moreover, the TVL administration established the oxidant/antioxidant balance, particularly in the chronic groups. Further studies are needed to investigate whether high doses of TVL have an effect on PTZ-induced seizure scores.
Investigation of the Pharmacological, Behavioral, and Biochemical Effects of Boron in Parkinson-Indicated Rats
(C M B Assoc, 2022) Ozdemir, Hulya Sagmanligil; Yunusoglu, Oruc; Sagmanligil, Vedat; Yasar, Semih; Colcimen, Nese; Goceroglu, Rezzan Temelli; Catalkaya, Ege
Parkinson's disease (PD) is a progressive neurodegenerative disorder of the central nervous system. In different studies, it has been investigated that boric acid has positive effects on different mechanisms that are important in PD. The aim of our study was to investigate the pharmacological, behavioral and biochemical effects of boric acid on rats with experimental PD with Rotenone. For this purpose, Wistar-albino rats were divided into 6 groups. Only normal saline was applied subcutaneously (s.c) to the first control and sunflower oil to the second control group. Rotenone was administered (s.c) to 4 groups (groups 3-6) at a dose of 2 mg/kg for 21 days. Only rotenone (2mg/kg, s.c) was administered to the third group. Boric acid was administered intraperitoneally (i.p.) at 5 mg/kg, 10 mg/kg, and 20 mg/kg to groups 4, 5, and 6, respectively. During the study, behavioral tests were applied to the rats, and then histopathological and biochemical analyzes were performed from the sacrificed tissues. According to the data obtained, a statistically significant difference (p<0.05) was observed between the Parkinson's group and the other groups in motor behavior tests, excluding the catalepsy test. Boric acid exhibited dose-dependent antioxidant activity. As a result of the histopathological and immunohistochemical (IHC) examination, a decrease in neuronal degeneration was observed at the increasing doses of boric acid, while gliosis and focal encephalomalacia were rarely encountered. There was a significant increase in tyrosine hydroxylase (TH) immunoreactivity, especially in group 6, with a dose of 20 mg/kg of boric acid. From these results, we conclude that the dose-dependent effect of boric acid may protect the dopaminergic system with antioxidant activity in the pathogenesis of PD. However, the effectiveness of boric acid on PD needs further investigation in a larger, more detailed study using different methods.