Browsing by Author "Sancak, T."

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Effect of Castration and Vasectomy on Some Oxidative Stress Parameters and Blood Hormone Levels in Rats
(Arquivo Brasileiro Medicina veterinaria Zootecnia, 2023) Sancak, T.; Kosal, V.; Okulmus, C.
Vasectomy and castration are the most preferred surgical methods to control reproduction in males. While sexual functions are terminated reversibly in vasectomy, they are removed irreversibly in castration. After these processes, changes are observed in hormones and oxidative stress parameters. In this study, we investigated the effects of vasectomy and castration operations on blood follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, nitric oxide (NO) and malondialdehyde (MDA) levels in rats. As a result of the analysis, it was determined that FSH, LH, NO, and MDA levels increased (p<0.05) and testosterone levels decreased (p<0.05) in the castration group compared to the sham and vasectomy groups. Considering the data obtained from the present study, when the two operations (vasectomy and castration) are compared in rats, which are preferred for the control of reproduction, it is thought that vasectomy is a healthier method because it is reversible, does not affect hormone levels, and does not increase oxidative stress.
Effect of Repeated Xylazine-Ketamine or Medetomidine-Ketamine Administration on Selected Reproductive Parameters in Male Rats
(Shiraz University, 2025) Okulmus, Ç.; Koşal, V.; Sancak, T.
Background: Sperm retrieval methods and surgical interventions are typically performed with the aid of anesthetic agents. In cases where the depth of anesthesia is insufficient, the procedure is often repeated. Aims: This study aimed to investigate the effects of repeated administration of xylazine-ketamine (XK) and medetomidine-ketamine (MK), as commonly used agents in laboratory animals, on sperm parameters, reproductive hormones, and testicular oxidative stress status. Methods: A total of 60 rats were used in the study. The animals were divided to two groups of thirty animals each as XK or MK groups, then each group was further divided into five subgroups (control, single dose, 1 repeat, 2 repeats, 3 repeats). Results: In the MK application group, the 2 repeats group exhibited a significant decrease in motility (P<0.001), an increase in the percent of abnormal sperms (P<0.05), and an elevated dead-to-live sperm ratio (P<0.001). In the MK 3 repeats group, a decrease in testosterone levels was observed (P<0.001). In all application groups (XK and MK), mRNA levels of testicular catalase (CAT), superoxide dismutase 1 (SOD1), androgen receptor (AR), and caspases 3/8/9 were significantly reduced (P<0.001). Conclusion: Despite the adverse effects of repeated anesthesia on certain testicular parameters, this study revealed that XK application is safer than MK in terms of spermatological characteristics. © 2025 Elsevier B.V., All rights reserved.