Browsing by Author "Sari, M"

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Induction and Synchronisation of Ovulation in Rainbow Trout, Oncorhynchus Mykiss, by Administration of Emulsified Buserelin (Gnrha) and Its Effects on Egg Quality
(Elsevier, 2004) Arabaci, M; Diler, I; Sari, M
The aim of this study was to evaluate the effects of a water in oil (w/o) emulsion of the gonadotropin-releasing hormone agonist buserelin ([D-Ser(tBu)(6), Pro(9)-NEt]-GnR-Ha) in Freund's incomplete adjuvant (GnRHa-FIA) on the induction and synchronisation of ovulation in an early spawning strain (late August-September) of rainbow trout, Oncorhynchus mykiss. Mature females were injected once with 0, 25, 50 mug kg(-1) of GnRHa-FIA or 25 mug kg(-1) of GnRHa in physiological saline (acute treatment). All fish dosed with 50 mug kg-1 of GnRHa-FIA ovulated within 10 days after injection, whereas fish dosed with 25 mug kg(-1) of GnRHa-FIA reached 100% ovulation 13 days after injection (water temperature, 11.5 degreesC). In contrast, ovulation percentage was 71% in control group, but it was only 66% in fish treated with acute injection of GnRHa 47 days after injection. Fertilization, eyeing and hatching percentages were high in all treatment groups and did not differ significantly among groups (p>0.05). None of the treatments caused any prespawning mortality. However, post-spawning mortality was observed in female rainbow trout treated with the low and high dose of GnRHa-FIA (46% and 53%, respectively). In conclusion, both GnRHa-FIA doses were effective in advancing the onset of ovulation, synchronising the ovulation of the treated fish and shortening the reproductive period, relative to the control or GnRHa injected group. GnRHa-FIA treatments did not affect oocyte diameter or egg quality based on the fertilization, eyeing and hatching percentages. There was, however, a very significant post-spawning mortality, reaching 50% of the population. (C) 2004 Elsevier B.V. All rights reserved.
Induction of Ovulation in Endemic Pearl Mullet (Chalcalburnus Tarichi), Living in the Highly Alkaline Lake Van, Using Gnrha([d-Ser(tbu)6, Pro9-Net] Combined With Haloperidol
(Elsevier, 2004) Arabaci, M; Sari, M
Pearl mullet (Chalcalburnus tarichi) (Cyprinidae) is an endemic fish species of the Lake Van (Turkey), the largest soda lake (pH 9.8) on earth. During April-June, the spawning period of the fish, the mullet migrate from Lake Van to freshwater rivers around the lake and after spawning they return to the lake. This fish gives hope for improving the fishery in the alkaline environment deprived of other commercial fish species. The objective of this study was to find an optimal method for inducing ovulation of pearl mullet. Mammalian GnRH analogue (GnRHa at 60 mug/kg); dopamine antagonist, haloperidol (HAL at 1.5 mg/kg) and a combination of GnRHa and haloperidol (GnRHa + HAL) at low (20 mug/kg GnRHa and 0.5 mg/kg HAL) and high (60 mug/kg GnRHa and 1.5 mg/kg HAL) doses were tested for this purpose. Saline (0.7% NaCl) injected fish were used as a control group and no ovulation occurred in this group. Based on spawning-ratio (the proportion of fish that spawned in each group), spawning index (the weight of eggs as a percentage of female body weight) and fertilization rate, the GnRHa + HA-L combination at the high (60 mug/kg GnRHa and 1.5 mg/kg HAL) doses were found to be more efficient (7/7, 16.0 +/- 2.4, 86.0 +/- 10 respectively) than the low (20 mug/kg GnRHa and 0.5 mg/kg HAL) doses of GnRHa + HAL (3/7, 12.4 +/- 2.1, 54.5 +/- 6.5 respectively). No females ovulated in the treatment group receiving HAL at 1.5 mg/kg and GnRHa alone at 60 mug/kg. It is concluded that GnRHa + HAL (60 mug/kg + 1.5 mg/kg) treatment is effective and reliable for induction of ovulation in pearl mullet broodstocks. Haloperidol improved the GnRHa effect on ovulation. GnRHa alone is not sufficient for spawning induction in the pearl mullet. The results indicate that in pearl mullet, similar to other cyprinids, there is a strong dopamine inhibitory tone on GtH secretion. In addition, the ovulation index and fertilization rates of eggs were influenced by the dosages of the applied GnRHa + HAL combination. (C) 2004 Elsevier B.V. All rights reserved.
Induction of Ovulation in Ornamental Common Carp (Koi, Cyprinus Carpio L.) Using Lhrha ([d-Ser(tbu)6, Pro9-Net] Combined With Haloperidol and Carp Pituitary Extract
(Blackwell Publishing Ltd, 2004) Arabaci, M; Çagirgan, H; Sari, M
The effects of a single injection of mammalian superactive analogue [D-Ser(tBu)(6),Pro(9)-NEt]-LHRHa (20 mug/ kg(-1)) combined with the dopamine antagonist, haloperidol (HAL, 0.5 mg kg(-1)), for induction of ovulation in the koi carp broodstocks were determined under routine hatchery conditions. The results were compared with classic carp pituitary extract (CPE, double injection) application (water temperature 22 degreesC). Physiological saline (0.7% NaCl)-injected fish were used as a control group and no ovulation occurred in this group. The spawning ratio was high in the LHRHa+HAL treatment group and in the CPE treatment group (6/7 and 5/7 respectively). The latency period was 14-16 h in the LHRHa+HAL treatment group and 12-14 h in the CPE treatment group (after the second injection). There was no difference between the two ovulating groups with respect to the spawning index (the weight of eggs as a percentage of female body weight) and fertilization rate of eggs (P>0.05). As a result, ovulation can be induced successfully in koi carp broodstocks with 20 mug kg(-1) [D-Ser(tBu)(6),Pro(9)-NEt]-LHRHa+0.5 mg kg(-1) HAL treatment in a single injection without decreasing the egg quality. Application of this combination can be useful for hatchery and broodstock management in koi carp culture.
Spawning Induction in the Common Carp (Cyprinus Carpio) Using Different Combinations of [d-Ser(tbu)6,pro9< and Metoclopramide
(Tubitak Scientific & Technological Research Council Turkey, 2004) Arabaci, M; Çagirgan, H; Sari, M; Gelmez, R
The effects of a single administration of [D-Ser(tBu)(6),Pro(9)-NEt]-LHRHa at high dose alone (50 mu g/kg), and at constant dose (20 mu g/kg) combined with different doses of the dopamine receptor antagonist Metoclopramide (5, 10, 20, 40 mg/kg) for the induction of spawning in common carp broodstocks were determined under local hatchery conditions and compared with classic carp pituitary extract (CPE, double injection) application (water temperature 24 degrees C). Fish injected with physiological saline (0.7% NaCl) were used as a control group. No spawning was observed in the control group or in the groups given LHRH-a combined with low (5, 10 mg/kg) doses of MET. The spawning ratio was low in the group given a high dose of LHRH-a alone (50 mu g/kg), while it was high in the groups given LHRH-a combined with high (20, 40 mg/kg) doses of MET, and in the group injected with CPE. Latency period in the LHRH-a+MET groups was approximately half of that in the CPE group (14-16 and 24-26 h, respectively). There were no differences between spawned groups with respect to the spawning index of broodstocks, or the fertilization/hatching rate of eggs. It was concluded that spawning can be induced successfully in common carp broodstocks with 20 mu g/kg LHRH-a+20 mg/kg MET, in a single injection, in a shorter latency period and without a negative effect on egg quality, compared to CPE treatment. This combination may be useful for more effective broodstock and hatchery management in common carp culture.
Water Pollution and the Levels of Gpt and Got Enzymes in Fish
(indian veterinary Journal, 2005) Ekici, K; Akkaya, L; Sari, M
Environmental pollution has negative effects on fish living in the sea. Increase in the amounts of serum GPT and GOT enzyme were generally observed (Heath, 1987). Meese (1990) stated that amounts of serum GPT and GOT ran change due to environmental factors in carps. In this study, an assessment was made to detect the water pollution by finding out the GPT and GOT enzymes levels in the serum and liver tissue of Chalcalbumus tafichi.