Browsing by Author "Sarikaya, Eren"

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Investigation of Levels of Oxidative Stress and Antioxidant Enzymes in Colon Cancers
(derman Medical Publ, 2017) Dusak, Ahmet; Atasoy, Nurhayat; Demir, Halit; Dogan, Erkan; Gursoy, Tulay; Sarikaya, Eren
Aim: Free radicals play a key role in the development of degenerative diseases, none the less, effects of harmful reactive species can be controlled by natural defense mechanisms in the body. In this study, we aimed to investigate enzyme activities of superoxide dismutase (SOD), glutathione (GSH) reductase, glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in blood serum samples of patients with colon cancer. Material and Method: This retrospective study included patients diagnosed with colon cancer (n=25) and age-and sex-matched healthy individuals as the control group (n=25). Serum levels of SOD, GSH, GSH-Px, and MDA were analyzed using the spectrophotometric method. Results: Enzyme levels of SOD, GSH, GSH-Px, and MDA were found to be statistically significantly (p< 0.05), compared to the control group. There was a significant increase in the MDA levels (p< 0.05), whereas the SOD, GSH, and GSH-Px levels decreased in the patients with colon cancer, compared to the control group (p< 0.05). MDA levels were significantly higher in the patients with colon cancer, while antioxidant enzymes including SOD, GSH, and GSH-Px activities were significantly lower in these patients, compared to the control group (p< 0.05). Discussion: Our study, for the first time, suggests a relationship between colon cancer, oxidative stress, and antioxidant parameters. This is also the first study to investigate the levels of oxidative stress levels and antioxidant enzymes such as SOD, GSH, and GSH-Px activities in the literature. Also, decreased GSH-Px activity may increase MDA production, suggesting that serum SOD, GSH, GSH-Px and MDA levels can affect the etiopathogenesis of colon cancer.
Investigation of Prolidase, Adenosine Deaminase, Glutathione S-Transferase and Glutathione Reductase Activities in Patients With Abortus Imminens
(derman Medical Publ, 2017) Turan, Kasim; Uckan, Kazim; Sarikaya, Eren; Demir, Halit; Demir, Canan
Aim: Abortus Imminens is a pregnancy-related disease, the pathophysiology of which has not yet been thoroughly determined. The aim of this study was to investigate possible relations between prolidase (PR) enzyme activity and adenosine deaminase (ADA), glutathione S-transferase (GST) and glutathione reductase (GR) activities in Abortus Imminens patients. Material and Method: Fifty patients with Abortus Imminens were examined in Ipekyolu Women's and Children's Hospital between June 2015 and September 2015. The age range of the patients selected was between 18 and 50. Prolidase (PR) enzyme activity and adenosine deaminase (ADA), glutathione S-transferase(GST) and glutathione reductase (GR) activities were measured in Abortus Imminens serums. Prolidase (PR), adenosine deaminase (ADA), glutathione-S-transferase (GST) and glutathione reductase (GR) activities were determined by spectrophotometry. Results: Glutathione S-transferase and glutathione reductase activities were found to be significantly decreased in Abortus Imminens patients compared to healthy subjects. Discussion: Changes in antioxidant enzyme activities may play a very important role in the pathogenesis of Abortus Imminens. The serum ADA levels can be used as a biochemical marker in Abortus Imminens. This is the first large-scale study in the literature of the relationship between Abortus Imminens and antioxidants.
Oxidative Stress Parameters and Their Correlation With Clinical, Metabolic and Polysomnographic Parameters in Severe Obstructive Sleep Apnea Syndrome
(E-century Publishing Corp, 2015) Asker, Selvi; Asker, Muntecep; Sarikaya, Eren; Sunnetcioglu, Aysel; Aslan, Mehmet; Demir, Halit
The aim of the present study was to assess the levels of oxidative stress markers, catalase (CAT), glutathione peroxidase (GPX) and malondialdehyde (MDA) in severe OSAS and to investigate any correlation between oxidative stress markers and clinical, metabolic and polysomnographic parameters. A total of 30 patients with severe OSAS and 30 healthy controls were included in this cross-sectional, clinical study. Demographic data, polysomnographic, biochemical and clinical indices as well as serum levels of CAT, MDA and GPX were measured and compared in OSAS and control groups. Furthermore, OSAS patients with and without pulmonary hypertension (PHT) were evaluated in terms of levels of CAT, MDA and GPX. Patients with severe OSAS exhibited significantly lower serum levels of CAT (P<0.001) and GPX (P<0.001). Serum MDA levels were remarkably higher in OSAS group (P<0.001). Correlation analysis revealed that levels of CAT and GPX were correlated with apnea-hypopnea index and there was a correlation between serum levels of MDA and CRP. Severe OSAS patients with and without PHT did not reveal any differences for CAT (P=0.789), MDA (P=0.805) and GPX levels (P=0.281). Our results have shown that oxidative stress markers significantly changed in patients with severe OSAS. This information is noteworthy because documentation of the role of oxidative stress in OSAS may have important implications regarding diagnosis, monitoring, treatment and prognosis.
Role of Oxidative Stress in Obese and Nonobese Pcos Patients
(Wiley, 2022) Uckan, Kazim; Demir, Halit; Turan, Kasim; Sarikaya, Eren; Demir, Canan
Objective. The aim of this study was to evaluate the relationship between the oxidant-antioxidant status, endothelial dysfunction, lipid metabolism, and metabolic syndrome risk in women with polycystic ovary syndrome (PCOS). Materials and Methods. Forty-five obese (BMI > 30 kg/m2) woman diagnosed with PCOS in the study, forty-five nonobese (BMI < 30 kg/m(2)) PCOS diagnosis working groups, and forty-nine healthy control groups were created with patients. Serum malondialdehyde (MDA) levels with antioxidant activities, such as SOD, GSH, GPx, and CAT activities, were measured by spectrophotometry. Results. There was a statistically significant difference in the mean serum MDA level in the obese PCOS group compared to the nonobese group and the control group (p < 0.001). When the antioxidant parameters, such as SOD, GPx, GSH, and CAT, were compared with the healthy control group, nonobese, and obese PCOS groups, the difference between the groups was statistically significant (p < 0.001). A positive correlation was observed between MDA and BMI, triglyceride, LDL, SBP, DBP, and HOMA-IR in the PCOS patient group. Conclusion. Oxidative stress and decreased antioxidant parameters in PCOS patients were correlated with hyperinsulinemia, hypertension, and dyslipidemia findings, and we think that this oxidative stress condition may contribute to metabolic syndrome and cardiovascular diseases in PCOS patients.