Browsing by Author "Sarikaya, Muecahit"

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The Acute Effects of Simulated Hypoxic Training at Different Altitudes on Oxidative Stress and Muscle Damage in Elite Long-Distance Runners
(Peerj inc, 2025) Sarikaya, Muecahit; Oge, Beyza; Embiyaoglu, Nuri Mert; Selcuk, Muzaffer; Cinar, Vedat; Oner, Salih; Batrakoulis, Alexios
Background Understanding the impact of altitude on muscle damage and oxidative stress is essential for optimizing training and recovery strategies for athletes exposed to high-altitude conditions. Therefore, this study aimed to investigate the effects of acute exercise at different altitudes on oxidative stress and muscle damage. Methods A total of twelve elite long-distance runners (mean age: 20.3 +/- 1.5 years) from different branches participated in the study. The exercise protocol was the Bruce submaximal treadmill exercise test, which was conducted under three simulated hypoxic conditions (at 1,700 m, 2,450 m, and 3,200 m) and one normoxic condition (sea level). All measurements took place at the same time of the day. After the exercise protocol, 5 ml venous blood samples were taken from the participants, while heart rate and oxygen saturation were monitored at the 3rd, 6th, 9th, and 12th minutes during the exercise. Results Significant altitude-dependent variations were observed in oxidative stress markers, with total oxidant status (TOS) (p = 0.017) and malondialdehyde (MDA) (p < 0.001) levels increasing at higher altitudes, while total antioxidant status (TAS) (p < 0.001) exhibited an elevation and oxidative stress index (OSI) (p < 0.001) demonstrated a decline as altitude increased. However, no significant difference was found in creatine kinase (CK, p = 0.059) levels. Additionally, there were significant differences in the oxygen saturation measurement taken at the 3rd (p < 0.001), 6th (p < 0.001), 9th (p < 0.001), and 12th (p < 0.001), minutes following the exercise session. There was no difference in the pulse measurement taken at the 3rd and 12th minutes, but a difference was observed at the 6th and 9th minutes post-exercise (p < 0.01). Conclusions In conclusion, the study determined that endurance exercises performed under simulated normobaric hypoxia at different altitudes increased TAS and reduced OSI in elite long-distance runners. The increase in TAS and the reduction in OSI were more pronounced at higher altitudes, particularly at 2,450 m and 3,200 m, compared to sea level. These findings highlight the need for altitude-specific training and recovery strategies to minimize oxidative stress and muscle damage in athletes.
Adaptation of the Effect of Chromium Mi̇neral Supplement on Serum Irisin, Leptin and Ghrelin Hormone Levels To Exercise Trainings
(Researchtrentz Acad Publ Education Services, 2022) Ulama, Mustafa Sencer; Sarikaya, Muecahit
This work; It is aimed to adapt the Effect of Chromium Mineral Supplement on Serum Irisin, Leptin and Ghrelin Hormone Levels to Exercise Trainings. For this purpose; A total of 28 male rats of eight weeks old Wistar Albino were included in the study and randomly divided into four groups. The control group (n=7) who continued to be fed with a daily diet and waited for eight weeks without any supplementation; The exercise group in which the treadmill exercise was performed at a speed of 15 m/min on the treadmill for five days for eight weeks (n=7); supplementation group (n=7) given chromium picolinate by gavage method (8 mu g / kg/day) once a day for eight weeks in addition to daily dietary nutrition; In addition to dietary nutrition, a single dose (8 mu g/kg/day) chromium picolinate was given by gavage for eight weeks and supplemented + exercise group (n=7) in which 20 min/day treadmill exercise was performed for eight weeks. At the end of the studies, which lasted for a total of 10 weeks with two weeks of adaptation exercises, blood samples were taken with the decapitations of the rats. Irisin, leptin and ghrelin analyzes in blood samples were studied by preparing them according to the kit procedure with the elisa method. According to the data we obtained, the normality homogeneity test was applied and it was determined that the data were normally distributed. As a result, one way anova test and paired sample t test were applied from parametric tests (p<0.05 statistically significant difference was accepted) and tukey test was applied to determine the difference between groups. SPSS-21 package program was used in the analysis of the data (p< 0.05 was considered a statistically significant difference). Correlation test was used to determine the relationship between variables. As a result, treadmill exercise applied at a speed of 20 minutes, 15 km/h, 5 days a week for eight weeks, and chromium mineral supplementation decreased the hormone level of ghrelin, thus reducing the need for food intake and less energy consumption. It was found that the use of chromium picolinate alone had a lowering effect on the leptin hormone, and that it also reduced the leptin hormone level in long-term exercise, that the combination of chromium picolinate supplement and exercise further reduced the already low leptin level compared to the chromium group and exercise group. We found that the level was significantly reduced.