Browsing by Author "Savas, Sumeyra"

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From Days To Hours: Can Maldi-Tof Ms System Replace Both Conventional and Molecular Typing Methods With New Cut Off Level for Vancomycin Resistant Enterococcus Faecium
(Elsevier, 2019) Savas, Sumeyra; Hazirolan, Gulsen; Karagoz, Alper; Parlak, Mehmet
Vancomycin-Resistant E. faecium (VRE) strains from clinical specimens were identified by conventional methods before. Following the phenotype-based identification, all strains were also identified using both BD Phoenix and VITEK MS bioMerieux System. Strains were typed with the Bruker MALDI-TOF MS system, pulsed field gel electrophoresis (PFGE) and 16S rRNA gene sequencing analysis and then the sensitivity compared for each. A cut off value of 850 assigned with Bruker MALDI-TOF MS system was found to give equal sensitivity to that of PFGE. Results obtained were compared with those of molecular typing. The main advantage of MALDI-TOF MS technology over the others was the much shorter analysis time which lasted only a few hours rather than days or a whole week. Also, the Bruker MALDI-TOF MS system was used for typing and compared with the gold standard method and this study is first to report the determined cut off level for typing of VRE strains.
Investigation of Some Changes and Clonal Relationship in Enterococci Isolates Due To Relocation of a Hospital
(Pakistan Medical Assoc, 2024) Korkoca, Hanifi; Hazirolan, Gulsen; Cicek, Cemal; Savas, Sumeyra; Akgul, Omer; Tanriverdi, Elif Seren
Objective: To investigate the isolation rates, antimicrobial resistance rates, minimum inhibitory concentration values of antimicrobial agents, and clonal relationships of Enterococcus faecalis and Enterococcus faecium due to the relocation of a hospital to a newly constructed building. Method: The comparative, prospective study was conducted at adult general intensive care units of the Mus State Hospital, Mus, Turkey, in two phases; before the relocation from January 25 to December 1, 2014, and after the relocation from February 10 to May 24, 2015. Rectal swab samples were collected 72 hours post-hospitalisation. Identification of Enterococcus faecalis and Enterococcus faecium isolates was determined by matrix -assisted laser desorption/ionization time -of -flight mass spectrometry, and antimicrobial resistance with minimum inhibitory concentration values was detected with Vitek 2 system. The clonal relatedness among the strains was investigated by pulsed -field gel electrophoresis. Data was analysed using SPSS 23. Results: Of the 69 patients, 37(53.62%) were related to pre -relocation phase; 20(54.1%) females and 17(45.9%) males with mean age 62.81 +/- 21.71 years. There were 32(46.37%) patients in the post -relocation phase; 13(40.6%) females and 19(59.4%) males with mean age 62.69 +/- 21.35 years (p>0.05). Of the 84 enterococci strains isolated, 51(60.7%) were Enterococcus faecium; 28(55%) before relocation and 23(45%) after relocation (p=0.77). The remaining 33(39.3%) isolates were Enterococcus faecalis; 16(48.5%) before relocation and 17(51.5%) after relocation (p=0.73). Multiple strains were located in 7(18.9%) patients before relocation and in 7(21.9%) after relocation. In 1(3.1%) patient after relocation, 2(8.7%) Enterococcus faecium isolates with different resistance and pulsed -field gel electrophoresis patterns were detected. There were no significant differences between the isolation and antibiotic resistance rates before and after relocation (p>0.05), and a clonal relation between the isolates was not detected (p>0.05). Decreased minimum inhibitory concentration values were noted for some antibiotics. Conclusion: Clonal relationship between the isolates and change in the rates of isolation and antimicrobial resistance of Enterococcus faecalis and Enterococcus faecium was not detected due to relocation. Minimum inhibitory concentration values could be used to reveal relocation -related changes in isolates obtained from patients hospitalised in intensive care units.
Molecular Characterization of Thermophilic Bacteria Isolated From Van City Ercis Town Hasanabdal Hot Spring
(Ars Docendi, 2009) Savas, Sumeyra; Adiguzel, Ahmet; Inan, Kadriye; Ozkan, Hakan; Gulluce, Medine; Sahin, Fikrettin
The study was carried am in order to determine the phenotypic and genotypic characterization of thermophilic bacteria isolated from Van city Ercis town Hasanabdal hot spring water. Turkey Fatty Acid, rep PCR profiling methods, and 16S rDNA sequence data were used for the molecular characterization of thermophilic bacteria, Totally 9 different bacterial isolates were selected based on morphological, physiological and biochemical tests. These strains were further characterized by molecular tests including fatty acid and rep PCR profiles, and 16S rDNA sequence. Its the Fatty Acid profiles were examined, it was determined that for P13 and P9 isolates, iso-C15:0. anteiso-C15:0. iso-C17:0 and anteiso-C17:0 fatty acids are the major fatty acids. For P22, P86, P56. P60. P59 and P4 isolates, the major fatty acids are C15:0. iso-C16:0, and iso-C17:0 fatty acids, and the major fatty acids for P14, P35 isolates are iso-C15:0 and anteiso-C15:0 fatty acids. These bacterial strains that differ in terms of fatty acid profiles were analyzed by REP and BOX-PCR, which are a genomic fingerprint analysis method. For the strains, which exhibited differences with respect to each other at the end of this analysis, 16S rDNA analyses were performed. As a result of 16S rDNA analyses, it was observed that six of these isolates were identified as Geobacillus pallidus and remaining four strains were Bacillus licheniformis, B. pumilus, Brevibacillus brevis and Br. borstelensis species respectively.