Browsing by Author "Sayan, Murat"

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Brucella Canis Coinfections in Patients With Brucellosis
(Aves, 2018) Sarigul, Figen; Erdenlig-Gurbilek, Sevil; Sayan, Murat; Tekin, Suda; Guducuoglu, Huseyin; Keskin, Oktay
Objective: Laboratory diagnosis of Brucella canis infections cannot be made by classical serological methods as readily as infections of other species of Brucella pathogenic for humans. Therefore, the information about B. canis infections in Turkey is limited. In this study, we aimed to detect presence of B. canis coinfections in humans who were diagnosed as brucellosis. Methods: Study has been designed as retrospective cross-sectional. A total of 566 serum samples collected from patients who had confirmed brucellosis and were living in cities of the Eastern and Southeastern Anatolia regions of Turkey were tested with rapid slide agglutination test (RSAT), 2-mercaptoethanol RSAT (2ME-RSAT) and microplate agglutination test (MAT) using B. canis M-, a less mucoid variant, as the antigen. Results: Out of the samples tested, 142 (25%) and 49 (8.7%) were positive by RSAT and 2ME-RSAT, respectively, and this difference was significant (p= 0.05). In total, 55/ 566 (9.7%) samples were MAT-positive. Differences between 2ME-RSAT and MAT were not statistically significant (p>0.05). Conclusions: Our B. canis coinfection prevalence data demonstrates that serological testing for B. canis should be performed in Turkey. It was also concluded that there are some advantages for using MAT, and this technique may be preferable over 2ME-RSAT.
A Serological Diagnostic Survey for Brucella Canis Infection in Turkish Patients With Brucellosis-Like Symptoms
(Natl inst infectious Diseases, 2011) Sayan, Murat; Erdenlig, Sevil; Stack, Judy; Kilic, Selcuk; Guducuoglu, Huseyin; Aksoy, Yavuz; Etiler, Nilay
The incidence of Brucella canis infection in humans is unknown in Turkey. In this study, we investigated the prevalence of B. canis infection in human sera obtained from six regions in Turkey and comparatively evaluated the results obtained by agglutination-based techniques using standardized antigens made from B. canis. The patients (n = 1,746) presented with clinical symptoms that were similar to those of brucellosis. All patients who tested negative in the Rose Bengal test for the smooth Brucella strains (abortus, melitensis, and suis) were screened for evidence of B. canis infection using the rapid slide agglutination test (RSAT), the microagglutination test (MAT), and the 2-mercaptoethanol RSAT test (2ME-RSAT). Of the samples tested, 157(8.9%), 68(3.8%), and 66(3.7%) were positive for B. canis, as determined by RSAT, MAT, and 2ME-RSAT, respectively. The diagnostic sensitivity, specificity, positive predictive value, and negative predictive value of RSAT were 100%, 94.6%, 42%, and 100%, respectively, and of MAT were 100%, 99.9%, 970, 70 and 100%, respectively. We recommend the routine use of MAT and 2ME-RSAT to check the sera of all patients with symptoms of brucellosis who are negative for brucellosis using a smooth Brucella antigen.