Browsing by Author "Seckin, Hamdullah"

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Antimicrobial, Antioxidant and Dna Damage Prevention Effect of Nano-Copper Particles Obtained From Diplotaenia Turcica Plant by Green Synthesis
(Hard, 2021) Seckin, Hamdullah
Metal nanoparticles have been intensely researched and developed in recent years due to their superior properties. There is a growing interest in economical and environmentally friendly techniques. Cu metal is preferred in research studies due to its cheapness and effects on human health (such as production of blood cells, oxidation and reduction). In recent years, the use of plant extracts in nanoparticle synthesis has become quite popular. In this study, we aimed to investigate the antioxidant capacity and protective properties of Cu nanoparticles obtained by using Diplotaenia turcica plant against damage to pBR322 plasmid DNA. In addition, we investigated the antimicrobial effect of Cu nanoparticles against Staphylococcus aureus ATTC 29213, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 10876, Escherichia coli ATCC 25952, Candida albicans ATCC 25952. Diplotaenia turcica plant characterization with Cu metal (Cu NPs/Dt) ultraviolet and visible light absorption spectroscopy (UV-vis), fourier-transformed infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersive X-ray (EDX) analysis. When looking at the antioxidant activity analysis, it is understood that it is a powerful antioxidant. It was determined that Cu NPs/Dt have significant antimicrobial activity. Its effect against some pathogens was found to be stronger than the positive control antibiotic. It was determined by the obtained DNA images that there is a potential to prevent breaks that may occur in DNA depending on the concentration.
Assessment of Therapeutic Potential of Silver Nanoparticles Synthesized by Ferula Pseudalliacea Rech. F. Plant
(Elsevier, 2022) Kocak, Yilmaz; Oto, Gokhan; Meydan, Ismet; Seckin, Hamdullah; Gur, Tugba; Aygun, Aysenur; Sen, Fatih
Plant-mediated synthesis of silver nanoparticles (Ag NPs) has increased its use in various biomedical applications due to its cost-effectiveness, renewable and environmentally friendly properties. Therefore, the focus of the study was to synthesize Ag NPs and to evaluate their antibacterial, and antioxidant activities by using the aqueous root extract of Ferula pseudodalliacea plant as a reducing agent. In addition, the synthesis of nanoparticles was confirmed by performing Ag NPs/Fp characterization processes. According to the findings of our research, color change due to surface plasmon resonance was confirmed by UV-vis spectrometry. The crystal properties of Ag NPs/Fp were determined according to the XRD model. Phytochemicals responsible for coating and reduction of Ag NPs/Fp were observed by FT-IR analysis. It has been shown that the therapeutic effect of Ag NPs/Fp exhibits better antimicrobial and antioxidant activity than aqueous extract. In addition, it was determined that Ag NPs/Fp structures showed the best antifungal effect against Candida albicans ATTC 90028 pathogen and gave a better zone than Rifampicin antibiotic. Therefore, studies at the molecular level and more comprehensively are required to determine the bioactive components and pharmacological effects of Ferula pseudodalliacea plant to confirm our antibacterial, antioxidant, and antifungal activity results.
Characterization of Rheum Ribes With Zno Nanoparticle and Its Antidiabetic, Antibacterial, Dna Damage Prevention and Lipid Peroxidation Prevention Activity of in Vitro
(Academic Press inc Elsevier Science, 2022) Meydan, Ismet; Burhan, Hakan; Gur, Tugba; Seckin, Hamdullah; Tanhaei, Bahareh; Sen, Fatih
This study aims to investigate the antidiabetic, antimicrobial, DNA damage, and lipid peroxidation prevention activity of ZnO NPs/Rr formed as a result of the interaction of Rheum ribes (R.ribes) plant with ZnO. The ZnO NPs/Rr obtained as a result of the reaction were confirmed using high-reliability characterization methods. According to the data obtained as a result of the study, it is seen that the activity of ZnO NPs/Rr to prevent lipid peroxidation is quite strong. Lipid peroxidation inhibition activity of ZnO NPs/Rr at the highest concentration of 250 mu g/ml was calculated as % 89.1028. It was observed that ZnO NPs/Rr prevented DNA damage by % 92.1240 at the highest concentration of 100 mu g/ml. It was determined that the antidiabetic effect of ZnO NPs/Rr formed by ZnO of R. ribes plant, which is used as a medicinal plant as an antidiabetic, was significant. It appears to have a strong antidiabetic property compared to the positive control acarbose. In our current study, it was observed that ZnO NPs/Rr formed zones ranging from 8 +/- 3.0 to 21 +/- 4.5 against Gram-positive and Gram-negative microorganisms. It has been determined that ZnO nanoparticles have an antibacterial effect.
Chitosan/Pva-supported Silver Nanoparticles for Azo Dyes Removal: Fabrication, Characterization, and Assessment of Antioxidant Activity
(Royal Soc Chemistry, 2024) Meydan, Ismet; Aygun, Aysenur; Tiri, Rima Nour Elhouda; Gur, Tugba; Kocak, Yilmaz; Seckin, Hamdullah; Sen, Fatih
With the advancement of technology, studies in the field of nanotechnology have attracted great interest in recent years. The fact that nanomaterials have superior advantages over micromaterials provides a wide range of uses. Green synthesis is an effective way to prepare nanomaterials with an easy, fast, and environmentally friendly method. Within the scope of the study, AgNPs were synthesized using basil extract and combined with chitosan/PVA as a support material. By using chitosan/PVA support materials, the surface area of AgNPs was increased and it was aimed to improve their properties. The synthesized AgNPs@chitosan/PVA nanocomposite was characterized using various methods. In the UV-Vis spectrum, an absorbance peak was observed at 430 nm for the AgNPs@chitosan/PVA nanocomposite, and the particle size was determined as 25.10 nm according to TEM results. In addition, the photocatalytic and antioxidant activities of AgNPs@chitosan/PVA nanocomposite were investigated. The antioxidant activity of the AgNPs@chitosan/PVA (100 mu g mL-1) nanocomposite against DPPH and H2O2 was determined as 89.18% and 71.87%, respectively. The photocatalytic activity of the AgNPs@chitosan/PVA nanocomposite against methylene blue (MB), methylene red (MR), methylene orange (MO), safranin, and crystal violet (CV) dyes was 77%, 85%, 79%, 54%, and 9%, respectively. While the highest photocatalytic activity was observed against MR dye, very low photocatalytic activity was observed for CV. In light of the results obtained, it can be said that the AgNPs@chitosan/PVA nanocomposite has the potential to be used as an antioxidant agent and photocatalyst. The photocatalytic activity of AgNPs@chitosan/PVA nanocomposite was tested against different dyes such as methylene blue, methylene orange, and methylene red as an effective photocatalyst.
Electrochemical Characterization of Vitamin B2 (Riboflavin) at Ag Nanoparticles Synthesized by Green Chemistry Dispersed on Reduced Graphene Oxide
(Elsevier Science Sa, 2023) Guler, Muhammet; Meydan, Ismet; Seckin, Hamdullah
The present study describes a novel electrochemical riboflavin (RF) sensor based on the green synthesis of silver (Ag) nanoparticles dispersed on reduced graphene oxide (rGO). The Ag nanoparticles was synthesized using Arum italicum leaf extract as reducing and stabilizing agent. The characterization of Ag nanoparticles was carried out using FT-IR, XRD, and SEM-EDX. The electrochemical studies were performed using cyclic voltammetry and differential pulse voltammetry. The Ag/rGO modified glassy carbon electrode (Ag/rGO/GCE) exhibited excellent electrochemical responses to the oxidation/reduction (redox) of RF. The sensor exhibited a linear determination range from 2 x 10-9 to 2.2 x 10-6 M with limit of detection (LOD) of 0.6 nM. The sensor had the sensitivity of 71.4 mu A mu M-1 cm-2. The sensor had satisfactory selectivity, reproducibility of 3.4 % and storage stability of 90.4 %. Also, the fabricated sensor was proved to be useful for the determination of RF in pharmaceutical samples.
An Environmental Approach for the Photodegradation of Toxic Pollutants From Wastewater Using Pt-Pd Nanoparticles: Antioxidant, Antibacterial and Lipid Peroxidation Inhibition Applications
(Academic Press inc Elsevier Science, 2022) Seckin, Hamdullah; Tiri, Rima Nour Elhouda; Meydan, Ismet; Aygun, Aysenur; Gunduz, Meliha Koldemir; Sen, Fatih
Background: Green synthesis is an effective and friendly method for the environment, especially in recent years has been used in many areas. It finds application opportunities in many fields such as physics, chemistry, electronics, food, and especially health and is the subject of intensive studies in this field. Objectives: The synthesized Pt-Pd NPs were aimed to be used as a bio-based photocatalyst under sunlight to prevent wastewater pollution. In addition, it is aimed to use Pt-Pd NPs as biological agents in different applications in the future. Methods: In this study, the platinum-palladium nanoparticles were synthesized by the extract of Hibiscus sabdariffa, the characterization of the nanoparticles was carried out by different methods (ultraviolet-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), infrared transform spectroscopy atomic force microscopy (AFM), and ray diffraction (XRD) analysis). And we discussed several different parameters related to human health by obtaining platinum-palladium bimetallic nanoparticles (Pt-Pd NPs) with a green synthesis method. These parameters are antioxidant properties (total phenolic, flavonoid, and DPPH scavenging activity), antibacterial activity, and lipid peroxidation inhibition activity. Gallic acid was used as standard phenolic, and quercetin was used as standard flavonoid reagents. The newly synthesized Hibiscus sabdariffa mediated green synthesized Pt-Pd NPs were compared with gram-positive and gram-negative bacteria, the high antibacterial activity was shown by gram-positive bacteria. The photodegradation of Pt-Pd NPs was carried out against MB dye for 180 min. Results: TEM results show that the average size of Pt-Pd NPs is around 4.40 nm. The total amount of phenolic compounds contained in 0.2 mg/ml of Pt-Pd NPs was equivalent to 14.962 +/- 7.890 mu g/ml gallic acid and the total amount of flavonoid component was found to be equal to 28.9986 +/- 0.204 mu g/ml quercetin. Hibiscus sabdariffa mediated green synthesized Pt-Pd NPs was found to have very effective for lipid pemxidation inhibition activity in the FeCl2-H2O2 system. The maximum DPPH scavenging activity was determined as 97.35% at 200 mu g/mi. The photocatalytic activity of Pt-Pd NPs was analysed against Methylene blue (MB) and the maximum degradation percentage was observed to be 83.46% at 180 min. Conclusions: The biogenic Pt-Pd NPs showed a high effective photocatalytic and biological activity.
Evaluation of Effectiveness of Neutral-Ph Superoxidized Solution (Nsos) With Peritoneal Lavage in Rat Fecal Peritonitis Model: an Experimental Study
(Kafkas Univ, veteriner Fakultesi dergisi, 2024) Aras, Abbas; Karaman, Erbil; Seckin, Hamdullah; Cibuk, Salih
This experimental study aimed to evaluate the effectiveness of NSOS (Neutral-pH superoxidized solution) with peritoneal lavage in rat fecal peritonitis models. Forty Wistar rats weighing between 250-300 g were used for the study. All rats were inducted for fecal peritonitis formation. The rats were divided into five groups as follows; Group 0: control, no intervention. Group 1: Application of 10 mg/kg NSOS into the peritoneal cavity after 6 h of induction of peritonitis. Group 2: 10 mg/kg NSOS application and repeated at 24 and 48 h. Group 3: NSOS + Antibiotic treatment (Seftriakson 30 mg/kg/ day IM 2x1 + metronidazol 15 mg/kg/day IM 2x1). Group 4: Only antibiotic treatment (Seftriakson 30 mg/kg/day IM 2x1 + metronidazol 15 mg/kg/day IM 2x1). The animals were examined for peritoneal and thoracal abscess formation, adherences, and any abnormality with inspection after sacrificing on the 7th day. The peritoneal lavage fluid culture for microbiological analysis and blood samples were taken for blood cultures, biochemical and infectious parameters of WBC, CRP, TNF- alpha, IL-6, IL-1 beta, and IL-10. Peritonitis was developed in all rats at the end of follow-up. No death was observed in rats on the seventh day of the experiment. Group 3 (NSOS + Antibiotic treatment) showed the most significant improvement in the infection of peritoneal fluid. NSOS and antibiotic together (Group 3) were found to be more effective against Klebsiella than Enterococcus sp. The blood cultures showed a significant reduction in all groups. The infectious parameters including IL-6, IL-1 beta, and IL-10 showed no significant difference in the first week of treatment between all groups. Only TNF- alpha was observed significantly lower in group 3 when compared to the other groups (P=0.001). Peritoneal lavage with neutral pH-superoxidized water plus an antibiotic regimen is the most effective treatment in the rat fecal peritonitis model. Further studies including human subjects are needed to investigate its effectiveness and validity
Green Synthesis, Characterization and Bioactivity of Biogenic Zinc Oxide Nanoparticles
(Academic Press inc Elsevier Science, 2022) Gur, Tugba; Meydan, Ismet; Seckin, Hamdullah; Bekmezci, Muhammed; Sen, Fatih
In this study, we tried to enlighten the structure of zinc oxide nanoparticles (ZnO NPs) obtained from Thymbra Spicata L. plant by using green synthesis method in various ways. Some properties of zinc oxide nanoparticles were determined by using the characterization methods that scanning electron microscopy (SEM), Energy Dispersive X-ray analysis (EDX), fouirer transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet visible spectroscopy (UV-Vis) spectroscopy methods. The detected Zn nanoparticle sizes were determined to be between 6.5 nm and 7.5 nm. In addition to these studies, we investigated the antimicrobial effects of zinc oxide nanoparticles obtained by green synthesis against some pathogens. According to the results, it was seen that zinc oxide nanoparticles formed zones with a diameter of 16.3 mm, 10.25 mm, 13 mm and 10.2 mm, respectively, against Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25952, Pseudomonas aeruginosa ATCC 27853 bacteria and Candida albicans ATTC 90028 fungus, respectively. However, the radical quenching activity (DPPH) of the nanoparticles (Ts-ZnONP (79.67%)) was determined to be quite good compared to the positive control BHA. In addition, it is seen that the protective effect of ZnO NPs against DNA damage increases depending on the concentration. At a concentration of 100 mg/L, the DNA damage inhibitory effect was found to be maximum. In line with the comprehensive results, it was determined that the zinc oxide nanoparticles obtained with the green synthesis method have the potential of use in a wide variety of fields.
Metagenomic Analysis of Intestinal Microbiota in Florated Rats
(Springernature, 2022) Komuroglu, Ahmet Ufuk; Seckin, Hamdullah; Ertas, Metin; Meydan, Ismet
Changes in gut microbiota have shown that it plays an important role in animal health and metabolic diseases. The intestinal microbiota is a complex structure that functions as an organ system with the presence of trillions of microorganisms. In this study, changes in the intestinal microbiota of Wistar rats with high fluorine were evaluated. Water containing 100 ppm NaF was given to 14 male Wistar albino rats as drinking water for 12 weeks. Fluorine is known to be an inducer of protein oxidation, lipid peroxidation, modulation of intracellular redox homeostasis, and oxidative stress. In this study, it was determined that the level of MDA (molandialdehyde), one of the oxidative stress parameters, increased significantly in the intestinal tissue after fluorine intoxication. The decrease in CAT (catalase) and SOD (superoxide dismutase) enzyme activities was found to be statistically significant. Intestinal tissues were taken under aseptic conditions and microorganisms found in flora were replicated by V3-V4 16S rRNA gene-specific primers. As a result of the sequence analysis, a statistical comparison of the control group and the fluorine applied group was made. The study we have done showed that there was a significant difference in species diversity in the intestinal microbiota of mice treated with fluorine. As a result, the composition of the intestinal microflora, especially Lactobacillus species, was significantly changed in rats with high fluorine.
Microflora in Rats Metagenomic Analysis of Intestinal Microflora in Rats Treated With Ellagic Acid and Sinapic Acid Using 16 S Rdna Gene Region
(Assoc Pharmaceutical Teachers india, 2022) Seckin, Hamdullah
Background: The correct functioning of the intestinal mechanism is essential for human health. In particular, the micro ecosystem of the gut has a vital role in the development of the host and the continuity of metabolism. For this reason, many studies have been conducted on the intestinal microbiota. Today, the use of phenolic compounds in the production of complementary and alternative medicines is quite common. Purpose: In our study, changes in intestinal microflora at the species level were evaluated in Wistar Albino rats treated with Ellagic Acid and Sinapic Acid. Materials and Methods: The first group was formed as the control group. Ellagic Acid (EA) was given to the second group of rats (50 mg/kg/d). The third group of rats was given Sinapic Acid (SA) (20 mg/kg/d). The fourth group of rats was given Ellagic Acid EA (50 mg/kg/d) and Sinapic Acid SA (20 mg/kg/d). At the end of the study, intestinal tissues were taken under appropriate sterile conditions. The V3-V4 points located in the 16 S rDNA gene regions of the bacteria in the microflora were replicated by the specific primers developed in accordance with these regions. Results and Conclusion: The data obtained from of sequencing analyzes were evaluated comparatively for four groups. As a result, it was determined that Ellagic Acid and Sinapic Acid have a positive effect on probiotic microorganisms (Lactobacillus) in the intestine. It was also determined that while it caused a decrease in the rate of some pathogenic microorganisms such as Streptococcus, it caused an increase in the number of some pathogens.
Synthesis and Characterization of Sophora Alopecuroides L. Green Synthesized of Ag Nanoparticles for the Antioxidant, Antimicrobial and Dna Damage Prevention Activity
(Univ Sao Paulo, Conjunto Quimicas, 2022) Seckin, Hamdullah; Meydan, Ismet
In this study, it was aimed to investigate the amount of antioxidant, protective properties against DNA damage and antibacterial properties against various pathogens after the interaction of Ag metal (Ag NPs/Sa) of Sophora alopecuroides L. (S. alopecuroides L) plant seed, which is grown in Igdir and used in the treatment of many diseases. The DPPH radical quenching activity of Ag NPs/Sa was performed by using Blois method, DNA damage prevention activity by gel electrophoresis and antibacterial property by disk diffusion method. With the green synthesis method, AgNPs obtained as a result of the reaction of the plant and Ag metal are UV visible spectrophotometer (UV-vis), fourier-transformed infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscope (SEM). DPPH radical quenching activity of Ag NPs/Sa was investigated in the concentration range of 25-250 mu g/ml. The radical quenching activity at a concentration of 250 mu g/ml was 85,215 +/- 0,101%, while this value was 93,018% for the positive control BHA. It has been observed that the protective property of pBR322 plasmid DNA damage against OH radicals originating from H2O2 increases with concentration. It has been observed that Ag NPs/Sa has significant antimicrobial properties against some pathogens (B. subtilis ATCC 6633 E. coli ATCC 25952, B. cereus ATCC 10876, P. aeruginosa ATCC 27853, E. faecalis ATCC 29212, S. aureus ATTC 29213 and C. albicans ATTC 90028) that cause disease and even some pathogens are more effective than antibiotics.