Browsing by Author "Sekeroglu, MR"

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Alterations in the Biochemical Markers of Renal Function After Sevoflurane Anaesthesia
(Wiley, 2005) Sekeroglu, MR; Kati, I; Noyan, T; Dülger, H; Yalçinkaya, A
Aim: This study has been carried out to see whether renal function is acutely altered in patients undergoing sevoflurane anaesthesia. For this purpose, the urinary levels of markers of renal tubular function, namely leucine amino peptidase (LAP), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and beta-2 microglobulin (beta-2M), and urinary albumin as a predictor of renal glomerular function were measured before and after sevoflurane anaesthesia. Methods: This study was comprised of 20 patients (11 males and nine females) aged 18-55, who underwent various elective surgical procedures under general anaesthesia. Urine samples of all patients were collected before and 1, 2 and 8 h after the anaesthesia. The levels of LAP, GGT, beta-2M, and albumin were then expressed as factored by urinary creatinine. In all patients, the anaesthesia was maintained with sevoflurane (2% end-tidal) at a high flow-rate (6 L/min). Results: Urinary beta-2M and LAP levels after anaesthesia were unchanged (P > 0.05). While urinary GGT and ALP levels were found elevated in the first hour, LDH levels were higher in the second hour (P < 0.05). They returned to normal levels in the later periods after the anaesthesia. Urinary albumin excretion (UAE) was significantly elevated in the second hour after the anaesthesia (P < 0.001). Although UAE was decreased in the eighth hour after the anaesthesia, it still remained higher than the pre-anaesthesia level (P < 0.001). Conclusion: These results suggest that a 2% end-tidal concentration of sevoflurane at a high flow-rate (6 L/min) acutely alters renal glomerular function but does not have a significant acute effect on biochemical markers of renal tubular damage.
Antithyroid Antibody Levels in Patients With Breast Cancer
(Amer Assoc Clinical Chemistry, 2004) Sekeroglu, MR; Dulger, H; Alici, S; Algun, E; Noyan, T
Blood Lipoperoxidation and Antioxidant Enzymes in Healthy Individuals: Relation To Age, Sex, Habits, Life Style and Environment
(Marcel dekker inc, 1997) Aslan, R; Sekeroglu, MR; Gultekin, F; Bayiroglu, F
We report the concentrations of superoxide dismutase (SOD), glutathione peroxidase (GSN-Px) and malonaldialdehyde (MDA) an end product of lipoperoxidation in human whole blood. Method for measuring was the based on spectrophotometer. Blood MDA level was higher in elderly than in children, adults. Smoking, alcohol taking, acute exercise and air pollution group's MDA levels were increased than their control groups. GSH-Px activity was significantly lower in acute exercise group, and higher in trained group than those of controls. SOD was decreased in elderly, smoking and acute exercise groups and increase in trained individuals. As a result the mean values we obtained were slightly significant in age groups. The life style, environment has polluted air and habits are thought to be responsible for these significance also.
Diagnostic Value of Cytokeratin-18 as a Tumor Marker in Bladder Cancer
(Pergamon-elsevier Science Ltd, 2002) Sekeroglu, MR; Aydin, S; Dülger, H; Yilmaz, Y; Bayrakli, H; Noyan, T
Objectives: The aim of the study was to compare serum levels of cytokeratin-18 of patients with bladder cancer with those of the healthy controls, and to investigate the relation between cytokeratin level and the tumor stage. Design and methods: Serum cytokeratin-18 levels of 38 patients with bladder cancer and of 25 healthy people were determined. Tumor stage was T-1 in 12 patients, T-2 in 9 patients, T-3 in 10 patients and T-4 in 7 patients. The serum cytokeratin-18 levels in these cases were analyzed with respect to the stage of the tumor. Results: Cytokeratin- 18 level in the patient group was found to be significantly higher than that of the control group (p < 0.010) when the groups were totally compared. However, when the levels in patients with different tumor stages were compared with that of the controls, the difference was not significant in patients with stage I and 2 tumors (p > 0.05). Regarding the cut off value as 4.0 ng/mL, sensitivity and specificity for serum cytokeratin-18 were found to be 53% and 72% respectively. When sensitivity was calculated with respect to tumor stages, it was 8% for T-1, 33% for T-2, 90% for T-3 and 100% for T-4. On the other hand, considering higher stage (T-3 and T-4) tumors only, the sensitivity was calculated as 94%, but the sensitivity for lower stage (T-1 and T-2) tumors was 19%. Conclusions: It is clear that serum cytokeratin- 18 level increases in patients with bladder cancer. However, it can only be useful as a tumor marker in the diagnosis of T-3 and higher staged tumors. This study indicated that cytokeratin- 18 does not have any diagnostic value in lower stage bladder cancers. (C) 2002 The Canadian Society of Clinical Chemists. All rights reserved.
The Effect of High Dose Digoxin on Cytokines in Healthy Dogs
(Hindawi Ltd, 2002) Dülger, H; Sekeroglu, MR; Eryonucu, B; Noyan, T; Alici, U
Background: Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta are pro-inflammatory cytokines, causing myocardial dysfunction and a negative inotropic effect. The drugs used to treat heart failure affect the production of cytokines. Digoxin, on which this study was focused, is one of the drugs for the treatment of heart failure. Aim: The present study was designed to examine the early effects of high doses of digoxin on the production of cytokines in healthy dogs. Methods: Digoxin was given parenterally to dogs at 0.15 mg/kg. IL-1beta and TNF-alpha production and levels of digoxin in the serum were measured 0, 12, 24, 48, and 72 h following administration of digoxin. Results: As the levels of serum digoxin taken at 12, 24, 48, and 72 h of administration were considered significantly high compared with preceding values (p<0.001), no notable change in serum IL-1 beta and TNF-alpha levels was observed. Conclusions: These results suggest that high doses of digoxin do not cause a significant cytokine production in heart muscle in the early phase.
Effects of Biological Extracellular Matrix Components on in Vitro Axonal Regeneration
(Amer Assoc Clinical Chemistry, 2005) Sekeroglu, MR; Ozturk, G; Baydas, B
Influence of Some Pesticides on Activity of Seven Serum Enzymes (In Vitro)
(Marcel dekker inc, 1997) Sekeroglu, MR; Celik, I; Arslan, O
The purpose of this study was to investigate the effects of Endosulfan [6,7,8,9, 10, 10-hexachloro- 1,5. 5a. 6, 9, 9 a hexahydro- 6, 5, methano - 2, 4, 3-benzo (e) dioxathpiepin 3- oxide], Mavrik [alpha-cyano -3-phenoxybenzyl M -(2 chloro-alpha-alpha-alpha-triflouro-p-tolyl)- D - valinate], 2,4-D (2,4-Dichlorophenoxyacetic acid) and Neoran (isopropyl -4,4-dibromobenzilate) on serum amylase (EC 3.2.1.1), creatine kinase (CK; EC 2.7.3.2) aspartate amino transferase (AST; EC 2.6.1.1), alanine aminotransferase (ALT; EC 2..6.1.2), alkaline phosfatase (ALP; EC 3.1.3.1), gamma glutamyl transferase (GGT; EC 2.3.2.2) and lactate dehydrogenase (LDH; EC 1.1.1.27). I-50 values of chemicals caused inhibition were determined by means of activity percentage -[I] diagrams. Endosulfan had effect of inhibition only on AST. The I-50 value of chemical was determined as 3.13x10(-4) M. Mavrik had effects of inhibition on AST, ALT, ALP and LDH. The values of I-50 were 7.83x10(-4) M, 1.17x10(-4), 1.23x10(-3) M and 2.23x10(-3) M, respectively. 2,4-D had effects of inhibition on ALT, ALP, GGT and LDH. The I-50 values of chemical were 6.97x10(-2) M, 5.05x10(-2) M, 2.35x10(-2) M, 1.07x10(-2) M, respectively. On the other hand, Neoran had effects of inhibition of all enzymes except amylase. I-50 values of this compound on other enzymes (CK, AST, ALT, ALP, GGT and LDH) were 4.41x10(-4) M, 5.06x10(-3) M, 2.40x10(-3) M, 5.23x10(-3) FA,4.4x10(-3) M, 5.97x10(-3) M, respectively.
Investigation of Malondialdehyde Formation and Antioxidant Enzyme Activity in Stored Blood
(Vsp Bv, 1997) Aslan, R; Sekeroglu, MR; Tarakcioglu, M; Koylu, H
In the present study, fresh blood obtained from six healthy adult male donors was investigated for erythrocyte malondialdehyde (MDA) formation and antioxidative enzyme activity. Plasma and erythrocyte membrane lipid peroxidation were measured by MDA formation. Erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels were determined in whole blood stored for transfusion purposes. Erythrocyte and plasma MDA levels increased significantly during the storage period from day 3 to day 19 and, after that, stayed unchanged. Erythrocyte MDA increased 100% on day 7 when compared to day 1. The GSH-Px activity significantly decreased after day 9 and SOD decreased after day 13. The reduction in enzyme activities continued until day 27. Our results showed significant alteration in erythrocyte membrane and plasma MDA formation and intracellular antioxidant enzyme status in whole blood used for transfusion. However, we do not know whether such alterations have clinical importances for the recipient.
Luteinizing Hormone Pulse Frequency and Amplitude in Azoospermic, Oligozoospermic and Normal Fertile Men in Turkey
(Wolters Kluwer Medknow Publications, 2002) Odabas, Ö; Atilla, MK; Yilmaz, Y; Sekeroglu, MR; Sengül, E; Aydin, S
Aim: To investigate the LH pulse frequency and amplitude in azoospermic and oligozoospermic patients and to compare them with normal fertile subjects. Methods: In this controlled clinical study, 10 normal fertile male volunteers and 20 infertile patients (10 oligozoospermic and 10 azoospermic) were enrolled. Blood samples were taken every 30 minutes for 12 hours. FSH, LH and T levels were determined. LH was observed at all the blood samples, but FSH and testosterone only at the first, middle and last samples. Results: The mean LH levels were significantly different between all the groups, but there was no statistical difference in the FSH levels between the fertile and oligozoospermic groups. The mean LH levels increased from the fertile towards the azoospermic groups (P<0.01). The LH pulse amplitude and frequency were significantly different between all the 3 groups. The former increased while the latter decreased from the fertile to the azoospermic groups. The T levels were different statistically only between the fertile and the azoospermic groups. Conclusion: The more prominent is the testicular defect, the lower will be the LH pulse frequency and the higher the amplitude.
Pro-Inflammatory Cytokines in Turkish Children With Protein-Energy Malnutrition
(Carfax Publishing, 2002) Dülger, H; Arik, M; Sekeroglu, MR; Tarakçioglu, M; Noyan, T; Cesur, Y; Balahoroglu, R
BACKGROUND: Protein-energy malnutrition (PEM) results from food insufficiency as well as from poor social and economic conditions. Development of PEM is due to insufficient nutrition. Children with PEM lose their resistance to infections because of a disordered immune system. It has been reported that the changes occurring in mediators referred to as cytokines; in the immune system may be indicators of the disorders associated with PEM. Aims: To determine the concentrations of pro-inflammatory cytokines in children with PEM, and to find out whether there was an association with the clinical presentation of PEM. Methods: The levels of serum total protein, albumin, tumour necrosis factor-a, and interleukin-6 were measured in 25 patients with PEM and in 18 healthy children as a control group. PEM was divided into two groups as kwashiorkor and marasmus. The kwashiorkor group consisted of 15 children and the marasinus group consisted of 10 children. Results: Levels of serum total protein and albumin of the kwashiorkor group were significantly lower than both the marasmus group and controls (p < 0.05). In view of tumour necrosis factor-a levels, there was no difference between groups (P > 0.05). While levels of interleukin-6 in both the marasmus group and the kwashiorkor group were significantly higher compared with controls (p < 0.05), there was no significant difference between the groups of marasmus and kwashiorkor (p > 0.05). Conclusions: It was observed that the inflammatory response had increased in children with malnutrition.
Serum Levels of Leptin and Proinflammatory Cytokines in Patients With Gastrointestinal Cancer
(Blackwell Publishing Ltd, 2004) Dülger, H; Alici, S; Sekeroglu, MR; Erkog, R; Özbek, H; Noyan, T; Yavuz, M
The aim was to investigate the serum levels of leptin, TNFalpha, IL-1 beta, IL-6, insulin, and growth hormone in patients with upper gastrointestinal cancer and cachexia. A total of 39 patients with various advanced stage (stage IV) gastrointestinal malignancies were enrolled. These cancer patients were divided into two groups according to the presence or absence of cachexia. Fifteen healthy adults were recruited as the control group. Body mass index (BMI; kg/m(2)) was calculated. Serum leptin, turnout necrosis factor (TNF)-alpha interleukin (IL)-1 beta, interleukin (IL)-6, growth hormone, insulin, glucose, triglyceride, total protein, albumin, erythrocyte sedimentation rate, and CRP were measured. In both cancer groups (cachectic and non-cachectic) body mass index and serum leptin levels were lower than controls (p < 0.001). Serum IL-1 beta, IL-6, and growth hormone levels were higher in both cachectic and non-cachectic groups than those of controls (p < 0.05). Serum TNF-alpha level in non-cachectic group was also significantly higher than in control group (p < 0.01). There is no significant difference between three groups in terms of insulin resistance as assessed by HOMA index. Our results showed that some proinflammatory cytokine levels were increased and leptin level was decreased due to upper gastrointestinal cancers. Increased cytokine levels may lead to decreased food intake and caused a weight loss. (C) 2004 Blackwell Ptiblishing Ltd.
The Serum Vitamin C Levels in Behcet's Disease
(Yonsei Univ Coll Medicine, 2003) Noyan, T; Sahin, I; Sekeroglu, MR; Dülger, H
Behcet's disease (BD) is a chronic inflammatory disorder of unknown aetiology, and recognised as a multi-system vasculitis. It has been postulated that an imbalance of the oxidant and antioxidant systems related to the disease are important in its pathogenesis. Previous publications have reported increased levels of enzymatic antioxidant defence systems in patients with BD. The non-enzymatic antioxidant systems, including vitamin C and uric acid, were looked for in the present study. For this aim, the serum malondialdehyde (MDA), an end product of lipid peroxidation, and vitamin C and uric acid, as endogenous antioxidants, were determined in 20 patients with BD (11 in active and 9 in inactive periods) and 20 healthy subjects. The MDA level was significantly higher in both the active and inactive period patients compared with the control group (p < 0.001, p < 0.05, respectively). The MDA level was also significantly higher in the active period patients compared with the inactive period patients (p < 0.05). The vitamin C levels were significantly lower in both the active and inactive period patients compared with the control group (p < 0.001, p < 0.05, respectively). There was no significant difference in the vitamin C level between the active and inactive period patients (p > 0.05). There was also no significant difference in uric acid levels between the groups (p > 0.05). In the patients group, a negative correlation was found between the levels of serum MDA and vitamin C (r=-0.517; p < 0.05). Our results indicate that decreased vitamin C and increased MDA levels reflect the increased levels of oxidative stress in BD patients, and this situation may be important in relation with its pathogenesis.
Sleep-Related Violence and Low Serum Cholesterol
(Blackwell Publishing Asia, 2002) Agargun, MY; Sekeroglu, MR; Kara, H; Özer, ÖA; Tombul, T; Kiran, Ü; Selvi, Y
To examine whether there is a relationship between serum cholesterol level and sleep-related violence, we evaluated 15 patients with violent behavior during sleep (VBS) and 15 normal control subjects. The patient and control groups were matched for sex, age, and weight. There were 13 women and two men in each group. The patients with VBS had lower serum total cholesterol, triglyceride, and low-density lipoprotein levels than the healthy subjects. Low cholesterol may effect serotonergic neuronal activity and some types of 5-HT receptors, then may be related to violent behavior during sleep.
Urinary Beta-2 Microglobulin in Renal Dysfunction Associated With Hypothyroidism
(Wiley, 2004) Algün, E; Topal, C; Öztürk, M; Sekeroglu, MR; Durmus, A
Hypothyroidism is associated with a number of functional renal disorders primarily affecting water and salt handling. In this study, we aim to investigate functional renal disorders in overt and subclinical hypothyroidism. We utilised urinary beta-2 microglobulin (beta2M) as a sensitive marker of tubular dysfunction. Urinary beta2M excretion and tests for renal functions were repeated before and after T4 replacement therapy. Forty-four patients (28 with overt disease, 16 with subclinical disease) and 31 healthy control subjects were involved in the study. There was a significant increase in urinary beta2M in both overt and subclinical hypothyroidism (p < 0.05 in both). TSH levels were correlated with beta2M excretion. Effects of hypothyroidism on renal functions were readily reversible by 3 weeks of thyroid hormone replacement therapy. Urinary beta2M was a sensitive marker of renal tubular dysfunction associated with overt and subclinical hypothyroidism. (C) 2004 Black-well Publishing Ltd.