Browsing by Author "Sepil, A."

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An Alternative Live Food for Fish Larvae; Vinegar Eels (Turbatrix Aceti) Enriched With Bacterial Protein
(Centenary University, 2019) Önalan, Ş.; Sepil, A.
Especially at commercial rearing of marine and ornamental fish species, the achievement of early stage feeding operations is very significant for reducing larval loses. For this reason, it is necessary to produce and use of live food organisms that appropriate for first mouth gap sizes of fish larvae. Widely used in this regard rotifer (Brachionus plicatilis) has got uneasy culture procedures, so evaluating alternative live food organisms is very important. In this study, we have assessed the feasibility of vinegar eels in early stage feeding of altricial fish larvae. Zebra fish (Danio rerio) have got smaller mouth gap size than other fish species have been used in feeding trials and acceptabilities of vinegar eels by larvae was evaluated. And also, an increase in protein contents of vinegar eel evaluated using bacterial proteins (Lactococcus garvieae). © 2019, Centenary University. All rights reserved.
Biological Characteristics of the Invasive Gambusia Holbrooki in the Wetland, the Lake Van Basin, Turkey
(Pleiades Publishing inc, 2022) Atici, A. A.; Sepil, A.; Sen, F.
Gambusia holbrooki (Girard, 1859) is worldwide spread invasive fish species and is found in the wetland, the Lake Van basin. This study was carried out to determine the biological characteristics of G. holbrooki. A total of 544 specimens were caught by electrofishing between August 2019 and July 2020. The maximum total length were 5.52 cm for females and 3.10 cm for males. The length-weight relationships were calculated as W = 0.014L(2.609) (R-2 = 0.808) for males (negative allometric growth) and W = 0.009L(3.110) (R-2 = 0.964) for females (positive allometric growth). The Fulton condition factor was determined as 1.00 +/- 0.01 (0.47-1.87). The female: male ratio was calculated as 1.75 : 1.00. The mean number of eggs for females was determined as 42.2 +/- 2.3 (15-94). Egg diameter ranged from 0.92 to 2.82 (mean 1.72 +/- 0.40) mm. The mean gonopodium length for males was 6.60 +/- 0.20 (5.90-7.20) mm. In general, due to high reproductive capacity and competitiveness, G. holbrooki are a serious threat to native freshwater fauna of the Lake Van Basin.
Embryogenesis and Early Larval Development in Rosy Barb (Pethia Conchonius, Hamilton 1822)
(Western Philippines University, 2025) Sepil, A.; Azizoglu, B.E.; Çekiç, Z.A.; Oguz, A.R.
This study aimed to investigate the larval ontogeny of rosy barb Pethia conchonius (Hamilton 1822). Embryonic and larval development stages were studied using the microscopy technique. Trials were carried out on 60 broodstock of rosy barb. Round and transparent fertilized eggs with a diameter of 867.6 ± 21.7 µm have started to hatch approximately 27 h after spawning at 26.3°C. The newly hatched larvae (n:30) have a mean total length of 4227 ± 265 µm and started exogenous feeding 4 days after hatching (DAH) and started taking particulate and powder feed at the end of 18 DAH. The early development growth formula of rosy barb has an exponential relationship model of y = 3.8346e0.0313x (R2 = 0.9112, n = 140). Digestive tract differentiation, hepatopancreas, digestive tubes, and gill arches formations were examined by taking histological sections in the early life stages of the rosy barb. Rosy barb is a species whose production protocol is not difficult due to its short egg hatching period and early larval stage zooplankton feeding period. © 2025, Western Philippines University.
First Report of Ligula Intestinalis (Cestoda: Pseudophyllidea) in Barbus Ercisianus (Cypriniformes: Cyprinidae) From the Nemrut Crater Lake, Turkey
(Centenary University, 2022) Önalan, Ş.; Atici, A.A.; Sepil, A.; Şen, F.
Van Basin has a rich geographical structure in terms of water resources and endemic fish species. In this study, Ligula intestinalis parasite, which is the host of Barbus ercisianus living in Nemrut Crater Lake, was recorded for the first time. The parasite was described molecularly as well as morphologically. In this context, a primer set was designed for molecular identification for the 28S rRNA gene region of L. intestinalis. Real-Time PCR results with the designed primers gave positive results in all parasite samples. The results were confirmed by a single peak (87) in the HRM analysis. © 2022, Centenary University. All rights reserved.
Histopathology of the Gills of Lake Van Fish Alburnus Tarichi (Güldenstädt, 1814) Infected With Dactylogyrus Spp. Parasites
(Western Philippines University, 2023) Erdemir, A.N.; Alkan, Z.; Azizoğlu, B.E.; Sepil, A.; Oğuz, E.K.; Oğuz, A.R.
The Lake Van fish is an anadromous carp endemic to the Lake Van basin. In this study, the histopathological effects of Dactylogyrus spp. parasites, which naturally infected fish, on the gills were determined during reproductive migration. Fish gills were stained with hematoxylin and eosin after routine histological procedures and apoptotic cells in the gills were determined immunohistochemically. Dactylogyrus spp. parasites were found in 8 of the 60 fish caught in the study. No parasites were found in the fish sampled from the lake. It was observed that the parasites caused hypertrophy, hyperplasia, edema, epithelial desquamation, hemorrhage, fusion of secondary lamellae, and necrosis in the gills. Immunohistochemically, no increase in the number of apoptotic cells was observed in the gills of the parasite-infected fish when compared with the non-infected fish gills. As a result, it can be inferred that the lake water has a restrictive effect in fish against parasites. Histopathologic lesions caused by the observed parasites in fish caught in fresh water could significantly affect gill functions. © Western Philippines University.
Ontogeny of Larval Pearl-Spotted Killifish Paraphanius Mento (Aphaniidae) Distributed in the Nemrut Crater Lake (Eastern Turkiye): a Histological and Microscopic Perspective
(Pleiades Publishing inc, 2024) Sepil, A.; Sen, F.
In this study, it was aimed to determine the larval ontogeny of Paraphanius mento (Heckel, 1843), which is distributed in Nemrut Crater Lake (Bitlis, Turkiye). Additionally, embryonic and larval development stages were evaluated microscopically and histologically. Filamentous and sticky fertilized eggs has a diameter of 1.251 +/- 0.316 mm with the inclusion of 8-12 oil droplets have started to hatch approximately 11 days after spawning at 23.5 degrees C. It was determined that newly hatched larvae have a total length of around 3.695 +/- 0.225 mm (n = 20), have started exogenous feeding at 3 day after hatching and started to take particulate baits at the end of 10 day after hatching. Digestive tract differentiation, hepatopancreas, digestive tubes and gill arches formations were examined by taking histological sections in early life stages.