Browsing by Author "Sepil, A."

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An Alternative Live Food for Fish Larvae; Vinegar Eels (Turbatrix Aceti) Enriched With Bacterial Protein
(Centenary University, 2019) Önalan, Ş.; Sepil, A.
Especially at commercial rearing of marine and ornamental fish species, the achievement of early stage feeding operations is very significant for reducing larval loses. For this reason, it is necessary to produce and use of live food organisms that appropriate for first mouth gap sizes of fish larvae. Widely used in this regard rotifer (Brachionus plicatilis) has got uneasy culture procedures, so evaluating alternative live food organisms is very important. In this study, we have assessed the feasibility of vinegar eels in early stage feeding of altricial fish larvae. Zebra fish (Danio rerio) have got smaller mouth gap size than other fish species have been used in feeding trials and acceptabilities of vinegar eels by larvae was evaluated. And also, an increase in protein contents of vinegar eel evaluated using bacterial proteins (Lactococcus garvieae). © 2019, Centenary University. All rights reserved.
Biological Characteristics of the Invasive Gambusia Holbrooki in the Wetland, the Lake Van Basin, Turkey
(Pleiades Publishing inc, 2022) Atici, A. A.; Sepil, A.; Sen, F.
Gambusia holbrooki (Girard, 1859) is worldwide spread invasive fish species and is found in the wetland, the Lake Van basin. This study was carried out to determine the biological characteristics of G. holbrooki. A total of 544 specimens were caught by electrofishing between August 2019 and July 2020. The maximum total length were 5.52 cm for females and 3.10 cm for males. The length-weight relationships were calculated as W = 0.014L(2.609) (R-2 = 0.808) for males (negative allometric growth) and W = 0.009L(3.110) (R-2 = 0.964) for females (positive allometric growth). The Fulton condition factor was determined as 1.00 +/- 0.01 (0.47-1.87). The female: male ratio was calculated as 1.75 : 1.00. The mean number of eggs for females was determined as 42.2 +/- 2.3 (15-94). Egg diameter ranged from 0.92 to 2.82 (mean 1.72 +/- 0.40) mm. The mean gonopodium length for males was 6.60 +/- 0.20 (5.90-7.20) mm. In general, due to high reproductive capacity and competitiveness, G. holbrooki are a serious threat to native freshwater fauna of the Lake Van Basin.
Comparison of Predicted and Measured Levels of Organic Material Input From a Commercial Cage Farm in Western Turkey
(Western Philippines University, 2022) Sepil, A.; Önal, U.
In this study, two different sediment trap trials were carried out to determine the deposition rate (flux) of particulate organic materials (POM) from marine cage farms. Flux predicted using a commercial software (Meramod), measured almost two-fold higher (1,355.5 g m-2 yr-1) in 2009 compared to that in 2008 (765.0 g m-2yr-1). Predicted levels of flux were higher than measured values in all trials and ranged between 1,251.6 g m-2 yr-1 in 2008 and 1,811.9 g m-2 yr-1 in 2009. There were also considerable differences in measured and predicted rates of flux at each station. High variations of flux in repeated measures indicated the need for strict control of farm maintenance routines during long-term trap studies. Near bottom current speeds, periodic resuspension events and the presence of wild fish assemblages were considered as major factors that may have effects in predicting the accumulation rates of POM. © 2022, Western Philippines University.
First Report of Ligula Intestinalis (Cestoda: Pseudophyllidea) in Barbus Ercisianus (Cypriniformes: Cyprinidae) From the Nemrut Crater Lake, Turkey
(Centenary University, 2022) Önalan, Ş.; Atici, A.A.; Sepil, A.; Şen, F.
Van Basin has a rich geographical structure in terms of water resources and endemic fish species. In this study, Ligula intestinalis parasite, which is the host of Barbus ercisianus living in Nemrut Crater Lake, was recorded for the first time. The parasite was described molecularly as well as morphologically. In this context, a primer set was designed for molecular identification for the 28S rRNA gene region of L. intestinalis. Real-Time PCR results with the designed primers gave positive results in all parasite samples. The results were confirmed by a single peak (87) in the HRM analysis. © 2022, Centenary University. All rights reserved.
Histopathology of the Gills of Lake Van Fish Alburnus Tarichi (Güldenstädt, 1814) Infected With Dactylogyrus Spp. Parasites
(Western Philippines University, 2023) Erdemir, A.N.; Alkan, Z.; Azizoğlu, B.E.; Sepil, A.; Oğuz, E.K.; Oğuz, A.R.
The Lake Van fish is an anadromous carp endemic to the Lake Van basin. In this study, the histopathological effects of Dactylogyrus spp. parasites, which naturally infected fish, on the gills were determined during reproductive migration. Fish gills were stained with hematoxylin and eosin after routine histological procedures and apoptotic cells in the gills were determined immunohistochemically. Dactylogyrus spp. parasites were found in 8 of the 60 fish caught in the study. No parasites were found in the fish sampled from the lake. It was observed that the parasites caused hypertrophy, hyperplasia, edema, epithelial desquamation, hemorrhage, fusion of secondary lamellae, and necrosis in the gills. Immunohistochemically, no increase in the number of apoptotic cells was observed in the gills of the parasite-infected fish when compared with the non-infected fish gills. As a result, it can be inferred that the lake water has a restrictive effect in fish against parasites. Histopathologic lesions caused by the observed parasites in fish caught in fresh water could significantly affect gill functions. © Western Philippines University.
Ontogeny of Larval Pearl-Spotted Killifish Paraphanius Mento (Aphaniidae) Distributed in the Nemrut Crater Lake (Eastern Turkiye): a Histological and Microscopic Perspective
(Pleiades Publishing inc, 2024) Sepil, A.; Sen, F.
In this study, it was aimed to determine the larval ontogeny of Paraphanius mento (Heckel, 1843), which is distributed in Nemrut Crater Lake (Bitlis, Turkiye). Additionally, embryonic and larval development stages were evaluated microscopically and histologically. Filamentous and sticky fertilized eggs has a diameter of 1.251 +/- 0.316 mm with the inclusion of 8-12 oil droplets have started to hatch approximately 11 days after spawning at 23.5 degrees C. It was determined that newly hatched larvae have a total length of around 3.695 +/- 0.225 mm (n = 20), have started exogenous feeding at 3 day after hatching and started to take particulate baits at the end of 10 day after hatching. Digestive tract differentiation, hepatopancreas, digestive tubes and gill arches formations were examined by taking histological sections in early life stages.