Browsing by Author "Seyhan, Ercan"

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The Effect of Pepper Gas (Oc) on Some Biochemical Parameters in Rats
(Kafkas Univ, veteriner Fakultesi dergisi, 2012) Seyhan, Ercan; Mert, Nihat; Mert, Handan
In this dissertation, the effect of the widely used pepper gas (oleoresin capsicum, OC) derived from the cayenne pepper was studied in the blood gases, electrolytes and some biochemical parameters on the rats with different dosages and periods. In the pre-dosage phase, 12 of 47 Wistar albino rats were used as the live material. Commercially available 90 g OC sprays were provided. It was experimentally measured that 6 g of OC was sprayed per second. 6 rats were used,as control group. Experimental Group 1 was 9 rats which were kept 5 min in an OC sprayed closed enviroment for 4 sec (24 g); Experimental Group 2 was 10 rats which were kept 8 min in an OC sprayed closed enviroment for 8 sec (48 g); Experimental Group 3 was 10 rats which were kept 15 min in an OC sprayed closed enviroment for 12 sec (72 g). The rats were kept as a group of 5 of each in 50x50x50 cm experiment cube and OC was applied for the abovementioned periods and dosages. The blood samples were taken from the heart after the 1-2 min sedation application. The potential hydrogen (pH), carbon dioxide partial pressure (pCO(2)), concentration of total carbon dioxide (ct CO2), O-2% saturation, HCO3, base excess (BE), base excess of extracellular fluid (B(ecf)), Na, K, Ca, Cl and glucose levels were measured by Rapidlab 865 System and auto analyser. When the control group values were compared to the other three groups, pH, ct CO2 and HCO3 (except the Group 3) were decreased, pCO(2), pO(2), O-2% saturation, K, Cl, glucose, BE and B(ecf) were increased and no significant change was observed in Na and Ca levels. The pH change (P <= 0.05), pO(2) (P <= 0.01), HCO3 (P <= 0.01); %O-2 saturation (P <= 0.001), K (P <= 0.05), Cl (P <= 0.05); B(ecf) (P <= 0.05) levels were evaulated the changes according to inter-group and control group. As a result, no fatality were encountered in rats which were exposed different dosages and amounts of OC in a closed enviroment except difficulty in breathing and conjuctivity. Despite that supporting biochemical changes for respiratoric asidose were encountered, it was understood that pepper gas could especially be used as a safe riot control agent in open air.
Effects of Genotype on Testis, Semen Quality, and Mineral Composition of Semen in Various Ram Breeds
(Humana Press inc, 2009) Mert, Handan; Karakus, Kadir; Yilmaz, Ayhan; Aygun, Turgut; Mert, Nihat; Apaydin, Betul; Seyhan, Ercan
This study was carried out on nine 3-5-year-old rams of different species: four Norduz, two Karakas, and three Ile de France x Akkaraman rams. Sperm of each ram was collected by means of an artificial vagina once per month from June to October 2007. In each case, the sperm volume, pH, viscosity, mass activity, motility, concentration, abnormal spermatozoa ratio, and the sperm death/live ratio were recorder along with the animals' testis diameter and scrotum circumferences. The concentrations of calcium, potassium, copper, magnesium, iron, and zinc levels in seminal plasma were determined. There were changes in the measured parameters according to genotype and time of sampling, which can be useful for better planning of anestrous mating programs for these ram species.
The Effect of Pepper Gas (OC) on Some Biochemical Parameters in Rats
(2010) Seyhan, Ercan; Mert, Nihat
Yıllardan beri kolluk kuvvetleri kargaşanın bastırılması ve saldırgan tavırların önlenmesinde farklı kimyasal ajanlar kullanmıştır. Cayenne bitkisinden(oleoresin capsicum)elde edilen ve bu amaçla çok kullanılan biber gazının, ratlar üzerinde farklı süre ve dozda uygulanması sırasında kan gazları, elektrolitler ve bazı biyokimyasal parametreler üzerine etkisi sunulan bu çalışmada incelendi. Araştırmada 12 tanesi ön doz çalışmasında kullanılmak üzere toplam 47 adet Wistar Albino rat canlı materyal olarak kullanıldı. Ticari olarak satılan 90 gr'lık spreyler halinde OC temin edildi. Saniyede 6 gr. OC püskürtüldüğü denemelerle hesaplandı. 6 adet rat kontrol grubu, 9 adet rat 4 saniye OC sıkılmış (24 gr) ortamda 5 dakika bırakılan Deneme 1; 10 adet rat 8 saniye (48 gr) OC sıkılmış ortamda 10 dakika bırakılan Deneme 2; 10 adet rat ise 12 saniye (72 gr) OC sıkılmış ortamda 15 dakika bırakılan deneme 3 grubunu oluşturdu. Ratlar 50x50x50 cm'lik bir deney küpüne beşerli gruplar halinde konularak, yukarıda belirtilen doz ve sürede OC uygulandı. Süre sonunda hayvanlar eter ile 1-2 dakika sedasyon işlemine tabii tutulup kalpten kan örnekleri alındı ve hemen pH, pCO2, ct CO2, %O2 saturasyonu, HCO3, BE, B(ecf), Na, K, Ca, Cl ve Glukoz düzeyleri Rapidlab 865 cihazı ve otoanalizörle ölçüldü. Kontrollerin değerleri diğer üç grup ile karşılaştırıldığında pH, ct CO3, üçüncü grup hariç HCO3 düzeylerinde azalma, pCO2, pO2, %O2saturasyonu, K, Cl, glukoz, BE ve B(ecf) düzeylerinde artma, Na, Ca düzeylerinde ise dikkati çeken değişim saptanmadı. pH değişimi (p? 0.05), pO2 p? 0.01, HCO3 p? 0.01, %O2 saturasyonunda p? 0.001, K p? 0.05, Cl p? 0.05, B(ecf) p? 0.05 düzeylerinde önemle deneme grupları arasında veya kontrollere göre değişim hesaplandı.Sonuç olarak kapalı ortamda farklı dozda OC miktarına, farklı sürelerde maruz kalan ratlarda solunum güçlüğü ve konjuktivit görülmesine rağmen hiçbir ölüm olayına rastlanılmamıştır. Kan gazları değerleri incelendiğinde respiratorik asidoz oluşumuna destek veren biyokimyasal değişimlere rastlanmış olmasına rağmen ve biber gazının özellikle açık alanlarda toplumsal olaylarda güvenli olarak kullanılabilecek gaz olabileceği kanısına varıldı.Anahtar Kelimeler, Biber gazı, Bikarbonat, Elektrolit, Kan gazları, Rat, OC.