Browsing by Author "Simsek, Aynur"

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Comparison of Tuberculin Skin Test, Ifn-Γ Assay, Real Time Pcr and Lateral Flow Rapid Test in Diagnosis of Field Outbreaks of Bovine Tuberculosis
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Kaya, Abdullah; Icen, Hasan; Tuzcu, Nevin; Simsek, Aynur; Yesilmen, Simten; Kochan, Akin; Alan, Adil
Bovine tuberculosis is an important zoonotic disease transmitted by direct contact, respiratory pathway, ingestion of unpasteurised milk and milk product, raw or undercooked meat. Tuberculosis can be difficult to diagnose based only on the clinical signs. Tuberculosis is usually diagnosed in the field with the tuberculin skin test. Sputum and other body fluids may be collected for microbiological examination. Polymerase chain reaction (PCR) methods have also been described. Diagnostic blood tests include the lymphocyte proliferation assay, the interferon gamma (IFN-gamma) assay, and enzyme-linked immunosorbent assays (ELISA). In this study a total of 50 animals were tested by using tuberculin skin test (TST), lateral flow rapid test, IFN-gamma assay and real time PCR. The animals were selected randomly among 178 cattle in dairy farms with the aged between 3-5 years and suspected of having tuberculosis. Forty five cattle were positive out of 50 for TST while 31 for reactive by the IFN-gamma assay and 28 for rapid test and 9 for real time PCR. The purpose behind such variable as age was to compare sensitivity of tuberculin skin test, the IFN-gamma assay and TB lateral flow rapid test and real time PCR examination for the diagnosis of field outbreaks of bovine tuberculosis in Turkey.
Diaphragmatic Hernia in a Sheep - a Case Report
(Univ Zagreb vet Faculty, 2018) Simsek, Aynur; Yaman, Turan; Icen, Hasan; Kochan, Akin
A two-year-old sheep was referred to the clinics of the Department of Internal Medicine of Dicle University, Faculty of Veterinary Medicine, with signs of anorexia, abdominal tympany and constipation. The clinical examination of the animal revealed the presence of respiratory failure associated with weaker lung sounds on the right side of the body when compared to the left side. There were no ruminal movements, and when the rumen was probed with a stomach tube, no ruminal content was obtained. The animal was euthanized upon the request of the owner, and a necropsy was performed. At necropsy, it was observed that the left hepatic lobe had protruded into the thoracic cavity through a defect in the diaphragm, and that a diaphragmatic hernia had developed.