Browsing by Author "Sipahioğlu, H.M."

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Detection and Characterization of Two Phytoplasma Lineages on Cucumber (Cucumis Sativus L.) With Same Symptomatology Based on Virtual Rflp and Nucleotide Sequence Analysis of 16s Rdna
(Centenary University, 2017) Usta, M.; Güller, A.; Sipahioğlu, H.M.
Phytoplasma-like symptoms were observed in cucumbers (Cucumis sativus L.) in Van province of Turkey. The major symptoms observed were severe dwarfing, witches’ broom, rosetting, little leaf, and sterility of plants. Genomic DNA of 8 symptomatic and non-symptomatic plant leaves was isolated for the detection of pathogenic DNA. Of the 8 cucumber leaf samples tested by nested polymerase chain reaction (Nested-PCR), the four yielded the expected 1.25-kb DNA fragments when using universal primer pairs R16mF2/R16mR1 and R16F2n/R16R2. Randomly selected two DNA bands were further cloned into a proper plasmid vector. The recombinant plasmid DNA was sequenced bidirectionally. BLAST and virtual restriction fragment length polymorphism (RFLP) analyses of the 16S rDNA sequence revealed the presence of the “Candidatus Phytoplasma solani” (similarity coefficient 1.00) (GenBank accession no: KX977570) in one of the severely symptomatic cucumber samples and the “Candidatus Phytoplasma trifolii” (similarity coefficient 0.98) (GenBank accession no.: KR080212) in the other. The isolates were designated as Van-trifolii and Van-solani isolates, respectively. No significant differences were observed between the two different phytoplasmas’ symptomatology. To the authors’ knowledge, this is the first report of ‘Ca. P. trifolii’ and ‘Ca. P. solani’ in cucumbers in Turkey. © 2017, Centenary University. All rights reserved.
First Report of ‘candidatus Phytoplasma Australasia’ Strain Related To Witches’-Broom of Tomato in Türkiye
(Centenary University, 2023) Usta, M.; Güller, A.; Sipahioğlu, H.M.
Phytoplasmas are dangerous bacteria severely infecting agricultural production worldwide. In the present study, the identification of phytoplasmas infecting tomato plants showing symptoms such as small leaves, flower abnormalities, stunting, witches' broom, and reddening was performed. Five plants, two symptomatic and three asymptomatic, were tested to verify phytoplasma infection. Total DNA isolated from 5 leaf samples was used as a template for PCR reactions. The phytoplasma agents were confirmed in the two symptomatic samples. BLASTn search of 16S rRNA of two sequences shared identity similarity of 99.84% with ‘Candidatus Phytoplasma australasia’. Computer-simulated virtual RFLP profiles show that the 16S rRNA sequences is identical to the reference pattern of the 16SrII-D subgroup, with a similarity coefficient of 1.00. Based on BLAST, virtual RFLP, and phylogenetic dendrogram, the identified phytoplasma strains are enclosed in the 16SrII-D subgroup. This is the first report of tomato witches' broom disease related to 16SrII-D subgroup phytoplasma strains in the Antalya province of Türkiye. © 2023, Centenary University. All rights reserved.
An Update on Phytoplasma Diseases Associated With Ornamentals in Asia
(Elsevier, 2023) Panda, P.; Rao, G.P.; Sipahioğlu, H.M.; Hemmati, C.; Madhupriya,; Kalita, M.K.; Kumar, P.
The economic importance of ornamentals has been progressing significantly in many Asian countries with international demand expanding continuously. Cut flowers represent the largest segment of the industry followed by flowering potted plants, trees, shrubs, annuals, flower bulbs, and other propagation materials. Like other crops, ornamental plants are also affected by several pathogens such as fungi, bacteria, viruses, phytoplasmas. Among them, the phytoplasmas are an important group of pathogens which drastically damage growth and marketing potentials of ornamental plants thereby affecting their commercial value. Phytoplasmas are associated with diseases in several commercial cut flowers and ornamental plants causing serious economic losses all over the Asia. They are a major constraint of phytoplasma infection in commercial production of ornamental plants by lowering the quantum and quality worldwide. The ‘Ca. P. asteris’ belonging to 16SrI group is the major group detected in ornamentals in Asian countries followed by peanut witches' broom (16SrII) group. Phytoplasma diseases of ornamentals in Asia have been described in a wide range of ornamental species, and phytoplasma associated belong to 11 different 16Sr groups and to about more than 27 different subgroups and are reported on more than 100 ornamental species. The major floriculture crops affected with phytoplasma diseases in Asia are roses, chrysanthemums, marigold, petunias, Chinese asters, calendulas, catharanthus, jasmines, and several seasonal ornamentals. The major number of phytoplasma reports associated with ornamentals are from India, Iran, Turkey, Japan, Korea, and China. Limited reports are available on natural reservoir sources of phytoplasma associated with ornamentals from Asian countries. Major management practices adapted for ornamental phytoplasma disease are using tetracycline treatment, insecticides for vector control and micropropagation of shoot tips for elimination of phytoplasmas. In this review, an update status of progress on research work done on phytoplasma diseases of ornamentals in Asia is discussed. © 2023 Elsevier Inc. All rights reserved.