Browsing by Author "Sipal, Sare"

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Investigation of Erbb-2 Overexpression on Patients With Gastric Cancer in Eastern Anatolia of Turkey
(Aves, 2013) Emre, Habib; Tekin, Salim Basol; Dogan, Hasan; Cayir, Kerim; Bilici, Mehmet; Sipal, Sare; Pirim, Ibrahim
Background/aims: In the present study, the frequency of ErbB-2 overexpression and its relationship with pathologic parameters on patients with gastric cancer in Eastern Anatolia of Turkey was studied. Materials and Methods: A total of 32 newly diagnosed patients were enrolled in the study. DNA isolation was performed on paraffinized tumor tissues obtained from patients by endoscopy or surgical resection. ErbB-2 overexpression was investigated from the isolated DNA by "Real Time Polymerase Chain Reaction". Results: ErbB-2 positivity was detected in five (15.6%) of 32 gastric cancer patients. The correlation between distant metastases and ErbB-2 positivity was found to be statistically significant (p=0.04). Additionally, no statistically significant correlation was noted between ErbB-2 positivity and parameters such as level of differentiation (p=0.7), the depth of tumor invasion (p=0.08), lymph node metastases (p=0.6), Lauren's classification (p=0.4), World Health Organization classification (p=0.3), tumor, node, metastasis staging (p=0.3) and tumor localization (p=0.2). Lymph node involvement was present in all ErbB-2 positive patients, the depth of tumor invasion was T3 (one case) and T4 (four cases) with the cardia being the most common location, which was remarkable, though not statistically significant (p>0.05). All ErbB-2 positive patients were detected with intestinal-type gastric cancer according to Lauren's classification and with the tubular-type according to World Health Organization classification. Conclusion: According to our findings, given the rates ofErbB-2 overexpression (15.6%) in gastric cancer, the investigation ofErbB-2 overexpression as an important biomarker in humanized monoclonal-antibody treatment in patients with gastric cancer was considered appropriate.
The Role of Cardiac Ganglia in the Prevention of Coronary Atherosclerosis: an Analytical Examination of Cholesterol-Fed Rabbits
(Galenos Publ House, 2020) Koza, Yavuzer; Aydin, Mehmet Dumlu; Bayram, Ednan; Sipal, Sare; Altas, Ender; Soyalp, Celaleddin; Koza, Enise Armagan
Background: The heart is innervated by the autonomic nervous system, which contributes to the control of the heart's rhythm and coronary circulation. It has been suggested that the cardiac fibers of the vagus nerve play important roles in controlling circulatory functions and in protecting against atherosclerotic pathologies in coronary arteries. Aims: To investigate the presence of atherosclerotic differences in the coronary arteries of cholesterol-fed rabbits by measuring the density of cardiac ganglia neurons. Study Design: Animal experiment. Methods: This study was conducted using 45 male rabbits. Over a period of 16 weeks, they were kept on an atherogenic diet of water ad libitum and high fat (8.6%) containing saturated fatty acids with 205 mg/kg of cholesterol (1%) per day. Then, their hearts were removed and examined by histopathological methods. Atherosclerotic plaques of the main coronary arteries were examined using the Cavalieri method. Atherosclerosis index values (AIVs) were estimated as the wall surface area/plaque surface area, and the results were analyzed with the Kruskal-Wallis and Mann-Whitney U tests. Results: While the average atherosclerosis index value was estimated to be <= 8% in 21 animals, the atherosclerosis index value was 9-20% in animals with minor plaque detection (n=11) and >= 20% in animals with major plaque detection (n=10). Increased atherosclerosis index values were more common in animals with low neuron densities than in animals with high neuron densities (p<0.017). Conclusion: The low neuron density of the cardiac ganglia in cholesterol-fed rabbits is associated with an increased atherosclerotic plaque incidence and volume.