Browsing by Author "Sirke, Sibel Turan"

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Characterization of a Diverse Okra (Abelmoschus Esculentus L. Moench) Germplasm Collection Based on Fruit Quality Traits
(Mdpi, 2025) Yildiz, Mehtap; Sirke, Sibel Turan; Kocak, Metin; Mancak, Ibrahim; Ozkaya, Aslihan Agar; Abak, Kazim; Cavagnaro, Pablo Federico
Okra is an important dietary component of many Asian countries, providing high levels of dietary fiber, phytonutrients (e.g., antioxidant vitamins and pigments), and essential minerals. Evaluation of okra germplasm collections can improve the curation of genebanks and help identify superior materials for breeding. In this study, 66 okra accessions from diverse geographical origins were characterized based on fruit quality traits, including fruit fresh (FFW) and dry weights (FDW), dry matter (DM), diameter, length, and diameter of the fruit peduncle; concentration of vitamin C, chlorophyll a and b, and total chlorophyll; and color-chroma values. Significant (p < 0.05) and substantial variation was found among the accessions for all traits. Mean FFW and FDW varied nearly three-fold, with ranges of 3.76-9.99 g and 0.43-1.34 g, respectively, with a range in DM content of 10.5-19.4%. Vitamin C and total chlorophyll content varied 6.4- and 8.3-fold, with ranges of 12.8-82.8 and 1.07-8.91 mg/100 g fw, respectively, with substantial variation also observed in chlorophyll composition. Significant positive correlations were found between vitamin C and total and subtypes of chlorophyll levels (r = 0.29-0.32), whereas the strongest correlations were between FFW and FDW (r = 0.88) and between total chlorophyll and chlorophyll subtypes a and b (r = 0.90-0.95). Additionally, a dendrogram constructed based on these phenotypic data grouped the accessions in general agreement with their geographical origins and fruit traits. Overall, our results revealed broad phenotypic diversity in the evaluated germplasm, which is exploitable in okra breeding programs aimed at increasing fruit quality and nutraceutical value.
Molecular Identification of Lactic Acid Bacteria From Traditional Fermented Foods and Screening Exopolysaccharide Production by Using Food Wastes
(Springer, 2024) Karaman, Kevser; Sirke, Sibel Turan; Rifaioglu, Seyda Nur Turkay
In this study, lactic acid bacteria (LAB) isolation from fermented foods and molecular identification using magnetic bead technology were performed. And then exopolysaccharide (EPS) production possibility was tested in agar medium, and the positive ones were selected for the next step. The bacteria that could produce higher carbohydrate level were grown in MRS medium fortified with whey and pumpkin waste. In our study, 19 different LAB species were identified from fermented products collected from different places in Hatay (T & uuml;rkiye) province. In molecular identification, universal primer pairs, p806R/p8FPL, and PEU7/DG74 were used for PCR amplification. After that, PCR products purified using paramagnetic bead technology were sequenced by the Sanger sequencing method. The dominant species, 23.8% of the isolates, were identified as Lactiplantibacillus plantarum. As a technological property of LAB, exopolysaccharide production capability of forty-two LAB isolate was tested in agar medium, and after eleven isolates were selected as positive. Two LAB (Latilactobacillus curvatus SHA2-3B and Loigolactobacillus coryniformis SHA6-3B) had higher EPS production capability when they were grown in MRS broth fortified with pumpkin waste and whey. The highest EPS content (1750 mg/L glucose equivalent) was determined in Loigolactobacillus coryniformis SHA6-3B grown in MRS broth fortified with 10% pumpkin waste. Besides the produced EPS samples were validated with FTIR and SEM methods.