Browsing by Author "Solmaz, H"

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Brucellosis in Horses Around Van, Turkey
(indian veterinary Journal, 2004) Solmaz, H; Tutuncu, M; Akkan, HA; Aksakal, A; Gulhan, T; Boynukara, B
A programme is being carried out in Turkey to eradicate brucellosis from cattle by compulsory slaughtering of positive cases on serological test surveillance. In spite of the very strict application of the programme, the results obtained so far are not satisfactory in respect of eliminating the disease. It seems that it will be very difficult to achieve this goal without considering the disease status in other domestic animals. Brucella abortus is associated with chronic inflammatory conditions including fistulous withers, poll-evil and also joint infection in horses. Consequently, in this study, to evaluate prevalence of brucellosis in horses, a comparative study of standard tube agglutination test (STAT) and Rose Bengal Plate Agglutination Test (RBPT) were carried out on horse sera.
Comparison of Rose Bengal Plate Test Antigens Prepared From Brucella Abortus, Brucella Melitensis and Brucella Suis
(Natl veterinary Research inst, 2005) Erganis, O; Hadimli, HH; Solmaz, H; Corlu, M
Rose bengal plate test (RBPT) antigens from Brucella melitensis and Brucella suis S 2 were prepared and compared with RBPT antigen prepared from classical Brucella abortus S 99. A total of 54 sera samples, of which 7 were collected from humans with brucellosis and 47 from infected sheep, were studied by using RBPT and serum agglutination test (SAT). RBPT and SAT results showed that RBPT antigens prepared from Br. melitensis and Br. suis S 2 were compatible with RBPT antigen prepared from Br. abortus S 99. Therefore, we concluded that RBPT antigens can be prepared from Br. melitensis and Br. suis S 2 and used for epidemiological surveilance of human and sheep brucellosis.
Detection of Campylobacter Antibodies in Sheep Sera by a Dot-Elisa Using Acid Extracts From C-Fetus Ssp Fetus and C-Jejuni Strains and Comparison With a Complement Fixation Test
(Wiley, 2002) Gürtürk, K; Ekin, IH; Aksakal, A; Solmaz, H
In this study, a dot-enzyme-linked immunosorbent assay (Dot-ELISA) was evaluated in comparison with a complement fixation test (CFT) for the detection of Campylobacter antibodies in sheep sera. Acid glycine extracts (AGE) of both Campylobacter ssp. fetus and Campylobacter jejuni strains that had been isolated from the gall-bladder of slaughtered sheep was used as antigen in both tests. A total of 153 sheep sera from aborted (74) and slaughtered (79) sheep were examined by both Dot-ELISA and CFT. Twenty-two sera showed anti-complementary activity were not suitable for CFT. Of the 22 sera showing anti-complementary activity, two sera were found to be positive in Dot-ELISA. Eighty-eight (67.2%) of the remaining 131 sera were negative by both Dot-ELISA and CFT using AGE of both Campylobacter strains whereas 43 sera (32.8%) gave different reaction patterns in Dot-ELISA and CFT with the extracts of both Campylobacter strains. Twelve sera were positive by both tests using AGE of C. fetus ssp. fetus but CFT failed to detect antibodies in nine of these sera when AGE of C. jejuni was used. Twelve sera were positive by both tests only when AGE of C. fetus ssp. fetus was used. Eleven sera were positive only, by CFT. Seven of these reacted only with the AGE of C. fetus ssp. fetus and four sera were positive by using AGE of both Campylobacter strains. The remaining eight sera were found to be positive only by dot-immunobinding assay either with the AGE of both Campylobacter strains or with the AGE of one of the Campylobacter strains. It is concluded that Dot-ELISA using AGE from C. fetus ssp. fetus could be employed for the detection of Campylobacter antibodies in sheep sera and the additional use of AGE from C. jejuni as antigen appeared not to be profitable for this purpose.
Investigation on Carriage of Clostridium Difficile in Van Cats
(indian veterinary Journal, 2003) Solmaz, H; Aksakal, A; Tutuncu, M; Akkan, HA; Boynukara, B
Seroprevalence of Brucellosis in Stray Dogs in the Region of Van, Turkey
(indian veterinary Journal, 2004) Solmaz, H; Tutuncu, M; Akkan, HA; Boynukara, B; Aksakal, A
Seroprevalence of Listeria Monocytogenes Infection in Cattle in Van, Turkey
(indian veterinary Journal, 2005) Tutuncu, M; Solmaz, H; Akkan, HA; Karaca, M; Agaoglu, Z
Listeria monocytogenes, the most common pathogenic species of the genus Listeria, causes listeriosis in both people and animals manifested by encephalitis, abortion and septicaemia. The main route of transmission of L. monocytogenes is by ingestion of contaminated food and the disease is particularly common in ruminants fed on silage (Unnerstad et al, 2000; Low and Donachie, 1997). The aim of this study was to determine seroprevelance of L. monocytogenes infection in cattle of the region Van, Turkey using LLO as antigen.
Vaccine Development From Serotypes 01, 02 and 078 of Escherichia Coli Against Avian Colibacillosis
(Scientific Technical Research Council Turkey, 2002) Erganis, O; Hadimli, HH; Solmaz, H
A trivalent Escherichia coli vaccine (TECA) was prepared from serotypes O1, O2 and O78 and one series of TECA was combined with an inactivated Newcastle Disease vaccine. TECA and combined vaccine were experimentally investigated for immunogenic effects in pullets and hens. This study was carried out at two stages consisting of rearing and laying periods: (1) A number of fourty chicks aged 15 days were subcutaneously vaccinated with 0.2 ml TECA. A similar group served as nonvaccinated controls. Half of the vaccinated group were revaccinated with 0.5 ml TECA at 71 days of age. The vaccinated and nonvaccinated pullets were challenged at 30 days after vaccinations. (2) Untreated breeder hens were vaccinated with TECA and combined vaccine by 0.5 ml, via SC route at 17 age of weeks. After vaccinations, the blood samples were taken from chicks/pullets with 15 days intervals and from the hens with 21 days intervals and then tested against O1, O2 ve O78 somatic "O" and pilus antigens by micro serum agglutination test (mSAT) and rapid agglutination test (RAT). The antibody titers in pullets vaccinated once or twice were found to be similar to0.5-3.6 times higher than controls, respectively. In breeder hens, the antibody titer figures were observed similar to1.5-4.8 times higher than those from nonvaccinated hens. By challange trials with homologous E. coli serotypes, the rates of protection in vaccinated and nonvaccinated pullets were found to be 77.8 and 46.7 %, respectively. The mortality rates of the chicks from the hens which had been vaccinated with TECA and combined vaccine or not been vaccinated were as follows: % 8.8. % 13.3 and % 50.3, respectively. In conclusion, TECH could be recommended for protection of both chicks and layers to E. coli infections. We also suggest that to comine inactivated E. coli with Newcastle vaccines could be useful.