Browsing by Author "Soylemez, Nihat"

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Accelerated Atherosclerosis in Haemodialysis Patients; Correlation of Endothelial Function With Oxidative Dna Damage
(Oxford Univ Press, 2012) Kaya, Yuksel; Ari, Elif; Demir, Halit; Soylemez, Nihat; Cebi, Aysegul; Alp, Hakan; Beytur, Ali
Background. Accelerated atherosclerosis is the major cause of mortality in patients on chronic haemodialysis (HD). The aim of this study was to evaluate the relationship between oxidative DNA damage [8-hydroxy-2'-deoxyguanosine/deoxyguanosine ratio (8-OHdG/dG ratio)], oxidative stress biomarkers and endothelial function in HD patients as an indicator of atherosclerosis. Methods. Forty-four chronic HD patients without known atherosclerotic disease and 55 age- and sex-matched healthy individuals were included in the study. Plasma malondialdehyde (MDA) levels and 8-OHdG/dG ratio were determined as oxidative stress markers. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were measured as antioxidants. Endothelial function was assessed by ultrasonography. Results. 8-OHdG/dG ratio and MDA levels were higher in HD patients than controls while SOD and GPx activities were lower in HD patients compared to controls. Flow-mediated dilatation FMD% in HD patients were lower than the control group (7.28 +/- 0.79 versus 11.18 +/- 0.82, P < 0.001). There was a significant negative correlation between FMD% and 8-OHdG/dG ratio (r = -0.678, P < 0.01) and MDA levels (r = 0.517, P < 0.01), while there was a significant positive correlation between FMD% and SOD (r = 0.538, P < 0.01) and GPx levels (r = 0.720, P < 0.01). Conclusions. Our data have demonstrated that HD patients exhibit increased oxidative DNA damage and decreased antioxidant activity. We propose that endothelial function is negatively correlated with 8-OHdG/dG ratio and positively correlated with antioxidant enzymes. To our knowledge, this is the first study to demonstrate the inverse relationship between endothelial function and plasma oxidative DNA damage in HD patients.
Correlations Between Oxidative Dna Damage, Oxidative Stress and Coenzyme Q10 in Patients With Coronary Artery Disease
(Ivyspring int Publ, 2012) Kaya, Yuksel; Cebi, Aysegul; Soylemez, Nihat; Demir, Halit; Hakan, Hamit A. L. P.; Bakan, Ebubekir
The correlation of coronary artery disease ( CAD) with pro-oxidant/antioxidant balance and oxidative DNA damage was investigated. Seventy-seven patients with CAD and 44 healthy individuals as control were included in this study. The comparative ratios of ubiquinol-10/ubiquinone-10, 8-hydroxy-2'-deoxyguanosine/ deoxyguanosine and the level of MDA measured by HPLC and the activities of GPX and SOD by colorimetric approach in blood samples obtained from patients with CAD were unraveled. 8-OHdG/dG ratios, serum MDA level and GPX activity were found significantly elevated level in serum of CAD patients compared to control group. The SOD activity was observed in stable levels in CAD patients. Ubiquinol-10/ubiquinone-10 ratio was significantly lower in patients with CAD than the controls. The positive correlation was observed between 8-OHdG/dG ratios in both MDA levels and GPX activity, while the significant negative correlation was seemed between the ratio of 8-OHdG/dG and ubiquinol-10/ ubiquinone-10 as well as MDA levels and ubiquinol-10/ubiquinone-10 ratio. We conclude that, both the disruption of pro-oxidant/antioxidant balance and oxidative stress in DNA may play an important role in the pathogenesis of coronary artery disease.
Percutaneous Closure of Secundum Atrial Septal Defects in Pediatric and Adult Patients: Short-, and Mid-Term Follow-Up Results
(Kare Publ, 2013) Kaya, Yuksel; Yurtdas, Mustafa; Ceylan, Yemlihan; Bulut, Mustafa Orhan; Soylemez, Nihat; Guvenc, Tolga Sinan; Ozkan, Mehmet
Objectives: We aimed to evaluate the shortand mid-term results of patients with atrial septal defect (ASD) who were treated with percutaneous closure. Study design: Seventy-nine patients with a diagnosis of secundum ASD (54 female and 25 male; mean age 26.2+/-17.2; range 3 to 71] years) were included in this study. All patients were evaluated by transthoracic (TTE) and/or transesophageal echocardiography (TEE). Amplatzer septal occluder (ASO) was used for percutaneous closure in all patients. In 76 patients, the procedure was performed under local anesthesia with TTE, while in the other 3 patients, it was performed with general anesthesia under the guidance of TEE. Patients were followed up at the 1st, 3rd, 6th and 12th months and annually thereafter. Mean followup period was 13.6+/-6.6 months. Results: Mean diameter of ASDs was 18.2+/-7.5 mm and stretched diameter was 20.7+/-8.04 mm during balloon dilatation, and mean diameter of implanted devices was 22.7+/-8.5 mm. Procedural time was 40.2+/-12.6, and fluoroscopy time was 10.9+/-4.1 minutes. The procedure was successfully performed in all patients (100%). One patient with cardiac tamponade died seven days after cardiac surgery. In two patients, the implanted devices embolized to the pulmonary circulation. Residual flow was found in three patients immediately after the procedure, without residual shunts one month after closure. Mild pericardial effusion in one patient and significant residual shunt due to device malposition in another were discovered at 1 and 6 months of the postprocedural follow- up period, respectively. Conclusion: Our findings showed that percutaneous closure of ASDs is successful in most patients with a low complication rate, and demonstrated that residual shunts do not develop in the majority of patients in the shortand mid- term.
Possible False Positive Heart Fatty Acid Binding Protein in Patient Who Has a High Level of Creatinine Kinase
(Aves, 2012) Acikalin, Ayca; Soylemez, Nihat; Kanadasi, Mehmet
Serum heart fatty acid binding protein (H-FABP) is a novel sensitive marker for early diagnosis of acute myocardial infarction. However, H-FABP is less specific than troponins, because it is also found in skeletal muscle. Recently, we have observed 3 patients who had high CK levels due to destruction of skeletal muscle and they had false positive high H-FABP levels.
Trace Elements, Heavy Metals and Vitamin Levels in Patients With Coronary Artery Disease
(Ivyspring int Publ, 2011) Cebi, Aysegul; Kaya, Yuksel; Gungor, Hasan; Demir, Halit; Yoruk, Ibrahim Hakki; Soylemez, Nihat; Tuncer, Mustafa
Aim: In the present study, we aimed to assess serum concentrations of zinc (Zn), copper (Cu), iron (Fe), cadmium (Cd), lead (Pb), manganese (Mn), vitamins A (retinol), D (cholecalciferol) and E (a-tocopherol) in patients with coronary artery disease (CAD) and to compare with healthy controls. Methods: A total of 30 CAD patients and 20 healthy subjects were included in this study. Atomic absorption spectrophotometry (UNICAM-929) was used to measure heavy metal and trace element concentrations. Serum a-tocopherol, retinol and cholecalciferol were measured simultaneously by high performance liquid chromatography (HPLC). Results: Demographic and baseline clinical characteristics were not statistically different between the groups. Serum concentrations of retinol (0.3521 +/- 0.1319 vs. 0.4313 +/- 0.0465 mmol/I, p=0.013), tocopherol (3.8630 +/- 1.3117 vs. 6.9124 +/- 1.0577 mmol/I, p<0.001), cholecalciferol (0.0209 +/- 0.0089 vs. 0.0304 +/- 0.0059 mmol/I, p<0.001) and Fe (0.5664 +/- 0.2360 vs. 1.0689 +/- 0,4452 mu g/dI, p<0.001) were significantly lower in CAD patients. In addition, while not statistically significant serum Cu (1.0164 +/- 0.2672 vs. 1.1934 +/- 0.4164 mu g/dI, p=0.073) concentrations were tended to be lower in patients with CAD, whereas serum lead (0.1449 +/- 0.0886 vs. 0.1019 +/- 0.0644 mu g/dI, p=0.069) concentrations tended to be higher. Conclusions: Serum level of trace elements and vitamins may be changed in patients with CAD. In this relatively small study we found that serum levels of retinol, tocopherol, cholecalciferol, iron and copper may be lower whereas serum lead concentrations may be increased in patients with CAD.