Browsing by Author "Suat, S."

Now showing 1 - 2 of 2

First Report on Determination of Bioelement, Vitamin Content and Antioxidant Properties of Verbascum Golawanense
(Pamukkale University, 2025) Bakir, A.; Suat, S.; Fırat, M.
This study was designed to investigate the antioxidant, antiradical potential and element and vitamin (A, E, K and C) levels in leaf extracts of Verbascum golawanense belonging to Scrophulariaceae family. For this purpose, DPPH, ABTS, anti-haemolytic activity, total flavonoid, phenolic content, total antioxidant capacity and vitamin C content were determined and measured spectrophotometrically. Vitamin A, E and K were analysed by HPLC and elemental analysis was performed by ICP-OES method. Phenolic, flavonoid and antioxidant capacity of the plant were determined as 5.861 ± 0.212 mg GA/g, 26.422 ± 0.613 mg QE/g and 124.359 ± 2.562 mM AA/g, respectively. K, Na, Fe, V, retinol, α-tocopherol, phylloquinone and vitamin C were determined as 3.086 ± 0.0131 mmol/kg, 2.260 ± 0.0266 mmol/kg, 1.1465 ± 0.0731 mmol/kg, 29.326 ± 3.072 µmol/kg, 0.1107 ± 0.013 µmol/kg, 0.4002 ± 0.16 µmol/kg, 1.101 ± 0.118 µmol/kg and 2552.0126 ± 187.056 mg/100g, respectively. The IC50 values related to the in vitro antioxidant properties of DPPH, ABTS and anti-haemolytic activity assays were 19.170 ± 0.615 μg/mL, 26.877 ± 0.461 μg/mL and 64.87 ± 2.24 μg/mL. In vitro studies revealed that the plant has a good antioxidant potential. It was determined that the vitamin and element content was high and total phenol, flavonoid and antioxidant potential were at reasonable levels. ABTS and DPPH tests showed a promising antioxidant power. These results indicated that the extract could be a potential alternative and could be investigated for the discovery and development of new chemical compounds for the treatment of various diseases. © 2025 Elsevier B.V., All rights reserved.
Investigation of Effects of Low Ruminal pH Values on Serum Concentrations of Macrominerals, Trace Elements, and Vitamins and Oxidative Status of Dairy Cows
(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Katsoulos, P.D.; Bilgiç, B.; Tarhan, D.; Ates, F.; Suat, S.; Kozat, S.; Dokuzeylul, B.
Due to the feeding system (high-concentrate diet) during the early lactation stage, ruminal pH in dairy cows follows a diurnal pattern and can remain below the critical level of 5.5 for extended periods of the day. This study aimed to evaluate the effect of low ruminal pH on blood concentrations of certain macrominerals, trace minerals, and fat-soluble vitamins and on the oxidative status of dairy cows during the first half of lactation. Fifty-three randomly selected lactating Holstein cows were used; blood and ruminal fluid samples were collected from all cows on days 30, 90 and 150 of lactation. Blood samples were obtained via coccygeal venipuncture, while the ruminal fluid was obtained by rumenocentesis and the pH was measured immediately after collection. Using a threshold pH of 5.5, samples were classified as normal (pH > 5.5) or low pH (pH ≤ 5.5). Serum concentrations of Ca, Mg, K, Cr, Mn, Zn, Se, and vitamins A, D3, E, and K were not significantly affected by ruminal pH, either by days in milk or by their interaction (p > 0.05). Plasma malondialdehyde and reduced glutathione followed the same trend (p > 0.05). Copper concentration was significantly higher (p < 0.05), and Fe concentration tended to be higher in cows with low pH compared to those with normal pH (p = 0.052). On day 150 of lactation, Cu, Fe, and Co concentrations were significantly higher in low-pH cows compared to normal-pH cows (p < 0.05). Low ruminal pH is associated with significant changes in serum concentrations of copper, iron, and cobalt but has no significant effect on the oxidative status of the animals or on the serum concentrations of the macro elements and fat-soluble vitamins studied. © 2025 Elsevier B.V., All rights reserved.