Browsing by Author "Suleyman, Bahadir"

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Comparative Investigation of Protective Effects of Metyrosine and Metoprolol Against Ketamine Cardiotoxicity in Rats
(Humana Press inc, 2015) Ahiskalioglu, Ali; Ince, Ilker; Aksoy, Mehmet; Ahiskalioglu, Elif Oral; Comez, Mehmet; Dostbil, Aysenur; Suleyman, Bahadir
This study investigated the effect of metyrosine against ketamine-induced cardiotoxicity in rats and compared the results with the effect of metoprolol. In this study, rats were divided into groups A, B and C. In group A, we investigated the effects of a single dose of metyrosine (150 mg/kg) and metoprolol (20 mg/kg) on single dose ketamine (60 mg/kg)-induced cardiotoxicity. In group B, we investigated the effect of metyrosine and metoprolol, which were given together with ketamine for 30 days. In group C, we investigated the effect of metyrosine and metoprolol given 15 days before ketamine and 30 days together with ketamine on ketamine cardiotoxicity. By the end of this process, we evaluated the effects of the levels of oxidant-antioxidant parameters such as MDA, MPO, 8-OHGua, tGSH, and SOD in addition to CK-MB and TP I on cardiotoxicity in rat heart tissue. The experimental results show that metyrosine prevented ketamine cardiotoxicity in groups A, B and C and metoprolol prevented it in only group C.
The Comparison of Resveratrol and N-Acetylcysteine on the Oxidative Kidney Damage Caused by High Dose Paracetamol
(Colegio Farmaceuticos Provincia de Buenos Aires, 2015) Yapanoglu, Turgut; Adanur, Senol; Ziypak, Tevfik; Arslan, Aynur; Kunak, Celalettin S.; Alp, Hamit H.; Suleyman, Bahadir
In this study, the effects of resveratrol were investigated on paracetamol-induced oxidative kidney damage in rats and compared with N-acetylcysteine (NAC). Rats were divided into healthy (HG), paracetamol control (PAC), resveratrol + paracetamol (RPA), NAC + paracetamol (NPA) groups. RPA group was administered 30 mg/kg resveratrol, NPA group was administered NAC in 300 mg/kg doses. Distilled water was administered to HG and PAC group. One hour after administration of the drugs and distilled water, all groups (except for HG) were given paracetamol 1000 mg/kg dose. 24 h after paracetamol administration, animals were killed by anesthesia and their kidneys were removed. Biochemical investigations were carried out on the kidneys. Resveratrol and NAC almost equally suppressed the increase of malondialdehyde (MDA) amount in the renal tissue of paracetamol-administered group. But resveratrol reduced the increase of 8-hydroxyguanine (8-OHGua), creatinine and blood urea nitrogen (BUN) more significantly than NAC. Resveratrol prevented loss of total glutathione (tGSH) in rats taking paracetamol better than NAC. As a result, resveratrol was determined to prevent oxidative renal damage and functional disorder related to paracetamol better than NAC.
The Effect of Metamizole on Ischemia/Reperfusion Injury in the Rat Ovary: an Analysis of Biochemistry, Molecular Gene Expression, and Histopathology
(Medknow Publications & Media Pvt Ltd, 2016) Kumbasar, Serkan; Salman, Suleyman; Al, Ragip Atakan; Ozturk, Cengiz; Yarali, Oguzhan; Alp, Hamit Hakan; Suleyman, Bahadir
Objectives: In this study, we investigated the effect of metamizole on ischemia/reperfusion (I/R) injury an analysis of biochemistry, molecular gene expression, and histopathology in the rat ovary of female albino Wistar rats. Materials and Methods: Animals were divided into four groups; control group with induced ischemia-reperfusion (IRC), ischemia-reperfusion 100 mg/kg metamizole sodium (MS) (IRM-100), ischemia-reperfusion 200 mg/kg MS (IRM-200), and healthy group applied sham operation (SG). Results: Myeloperoxidase (MPO) activity and gene expression increased significantly in IRC and IRM-100 group rat ovarian tissue compared with the SG group (P < 0.0001). However, MPO activity and gene expression in IRM-200 group ovarian tissue decreased significantly compared with the IRC and IRM-100 groups (P < 0.0001). Histopathologically, pronounced congestion, dilated vessels, hemorrhage, edema, degenerative cells, and neutrophil migration and adhesion to the endothelium were observed in the IRC and IRM-100 group ovarian tissues. A small number of congested dilated vessels, mild congestion, and edema were observed in the IRM-200 group, but no neutrophil migration and adhesion to the endothelium or degenerative cells. Conclusions: At 200 mg/kg dose metamizole prevented ovarian injury induced with I/R. This data show that metamizole can be used in the ovarian I/R injury treatment.
The Effect of Thiamine Pyrophosphate on Ethambutol-Induced Ocular Toxicity
(Taylor & Francis Ltd, 2016) Cinici, Emine; Cetin, Nihal; Ahiskali, Ibrahim; Suleyman, Bahadir; Altuner, Durdu; Alp, Hamit Hakan; Suleyman, Halis
Context: Ethambutol-induced retinal oxidative damage in patients with tuberculosis is still not being adequately treated. The protective effect of thiamine pyrophosphate against oxidative damage in some tissues has been reported, but no information on the protective effects of thiamine pyrophosphate against ethambutol-induced oxidative retinal damage has been found in the medical literature.Objective: The objective is to investigate whether thiamine pyrophosphate has a protective effect against oxidative retinal damage in rats induced by ethambutol.Materials and methods: Experimental animals divided into four groups (n=10): the healthy group (HG), the ethambutol control group (EMB), thiamine+ethambutol group (Thi-EMB) and thiamine pyrophosphate+ethambutol group (TPP-EMB). The rats in the TPP-EMB and Thi-EMB groups were administered thiamine pyrophosphate and thiamine, respectively, at doses of 20mg/kg intraperitoneally. Distilled water was administered intraperitoneally to the HG and the EMB groups as a solvent in the same volumes. One hour after drug injection, 30mg/kg ethambutol was administered via an oral gavage to the TPP-EMB, Thi-EMB and EMB groups. This procedure was repeated once a day for 90 days. At the end of this period, all rats were euthanized under high-dose thiopental sodium anesthesia, and biochemical and histopathological investigations of the retinal tissue were performed.Results: Malondialdehyde (MDA) and DNA damage product 8-hydroxyguanine levels were significantly lower in the retinal tissue of TPP-EMB and HG groups compared to those of the Thi-EMB and EMB groups, and total glutathione (tGSH) was also found to be higher. In addition, severe retinal tissue vascularization, edema and loss of ganglion cells were observed in the Thi-EMB and EMB groups, whereas histopathological findings for the TPP-EMB group were observed to be close to normal.Discussion and conclusion: These findings suggest that thiamine pyrophosphate protects retinal tissues from ethambutol-induced oxidative damage, and thiamine does not. This positive effect of thiamine pyrophosphate may be useful in the prevention of ocular toxicity that occurs during ethambutol use.
Molecular Mechanism of the Protective Effect of Adenosine Triphosphate Against Paracetamol-Induced Liver Toxicity in Rats
(Aepress Sro, 2023) Koc, Alparslan; Gazi, Mustafa; Sayar, Ali Caner; Onk, Didem; Ari, Muhammet Ali; Suleyman, Bahadir; Suleyman, Halis
Toxic doses of paracetamol are also known to be close to therapeutic doses. This study aimed to biochemically investigate the protective effect of ATP against paracetamol-induced oxida-tive liver injury in rats and to examine the tissues histopathologically. We divided the animals into the paracetamol alone (PCT), ATP + paracetamol (PATP), and healthy control (HG) groups. Liver tissues were examined biochemically and histopathologically. Malondialdehyde level, AST and ALT activity in the PCT group were significantly higher than those in the HG and PATP groups (p < 0.001). The glutathione (tGSH) level, superoxide dismutase (SOD) and catalase (CAT) activity in the PCT group was significantly lower than that in the HG and PATP groups (p < 0.001), while animal SOD activity was significantly different between the PATP and HG groups (p < 0.001). The activity of CAT was almost the same. In the group treated with paracetamol alone, lipid deposition, necrosis, fibrosis, and grade 3 hydropic degeneration were observed. No histopathological damage was observed of the ATP-treated group, except for grade 2 edema. We discovered that ATP reduces the oxidative stress caused by paracetamol ingestion and protects against paracetamol-induced liver injury at the macroscopic and histological levels.
The Protective Effect of Melatonin and Agomelatin Against Cisplatin-Induced Nephrotoxicity and Oxidative Stress in the Rat Kidney
(Colegio Farmaceuticos Provincia de Buenos Aires, 2013) Yilmaz, Ismayil; Demiryilmaz, Ismail; Turan, Mehmet I.; Suleyman, Bahadir; Turan, Isil S.; Altuner, Durdu; Suleyman, Halis
Cisplatin is used to treat various types of cancers. Its use is limited, however, due to nephrotoxicity, which may result from free radical damage. Evidence exists that melatonin reduces oxidative stress-induced damage. This study investigated the protective effect of agomelatin, a melatonin receptor agonist, against cisplatin-induced nephrotoxicity and oxidative stress in the rat kidney. Groups of rats were given cisplatin with or without agomelatin or melatonin, or distilled water for 14 days. MDA, tGSH, MPO and 8-OH Gua levels were measured to determine oxidative and DNA damage in renal tissue. Levels of MDA, MPO and 8-OH Gua were lower in the Mel+Cis and Ago+Cis groups than in the Cis group (P < 0.001, P < 0.001, and P < 0.05, respectively). The tGSH level in the Mel+Cis group was higher than that in the Cis group (P < 0.001). Agomelatin and melatonin thus reduced cisplatin-induced oxidative damage and DNA damage in the rat kidney. This suggests that melatonin may be effective in preventing cisplatin nephrotoxicity.