Browsing by Author "Sunnetcioglu, M."

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Association of Paraoxonase Activity and Atherosclerosis in Patients With Chronic Hepatitis B
(Makerere Univ, Fac Med, 2012) Karsen, H.; Binici, I; Sunnetcioglu, M.; Baran, A., I; Ceylan, M. R.; Selek, S.; Celik, H.
Background: The hepatitis B virus is a significant pathogen that causes cirrhosis, and hepatocellular cancer as a result of the damage it causes to liver cells. Its infection affects more than 400 million people globally. Although there is an effective vaccine and treatment methods, almost 1, 000, 000 people die every year. Objective: To investigate paraoxonase and arylesterase activities along with oxidative status parameters and serum lipid levels, and to find out if there is any increased susceptibility to atherogenesis. Methods: Thirty-four subjects with chronic hepatitis B and 39 healthy subjects as control were enrolled in the study. Age, body mass index and gender, Serum Triglycerides (TG), High-density Lipoprotein (HDL) and Low-Density lipoprotein (LDL) levels, serum paraoxonase-1 and arylesterase activities were determined. Oxidative and antioxidative statuses were evaluated by measuring serum-free sulfhydryl groups, lipid hydroperoxide levels, total antioxidant capacity, total oxidant status, and oxidative stress index. Results: Serum TG and LDL levels were higher while serum HDL levels were lower in patients with chronic hepatitis B than in controls but the differences did not reach statistical significance. Serum paraoxonase-1 and arylesterase activities, plasma free sulfhydryl groups, and total antioxidant capacity were significantly lower in patients than in controls (p=0.018, p=0.005, p<0.001, p=0.037 respectively), while lipid hydroperoxide, total oxidant status, and oxidative stress index were significantly higher (for all p<0.001). Conclusion: The diminution in the paraoxonase-1 and arylesterase activities could contribute to the accelerated development of atherosclerosis in patients with chronic hepatitis B.
Characterisation of Drug Resistance of Nosocomial Esbl-Producing E. Coli Isolates Obtained From a Turkish University Hospital Between 2009 and 2012 by Pulsed Field Gel Electrophoresis and Antibiotic Resistance Tests
(EDIMES Edizioni Medico Scientifiche, 2016) Karagöz, A.; Sunnetcioglu, M.; Ceylan, M.R.; Bayram, Y.; Yalcin, G.; Kocak, N.; Andac, C.A.
In this study, drug resistance of 28 ESBL-producing Escherichia coli isolates obtained from 144 patients hospitalized at the Yüzüncüyil University Hospital at Van (YUH), Turkey, between 2009 and 2012 were characterized by pulsed field gel electrophoresis and antibiotic susceptibility tests. Antibiotic resistance profile was determined by a Phoenix automated system (BD, USA). The ratio of ESBL-producing E. coli strains was determined to be 19.4% (28 out of 144 E. coli isolates). It was determined that the anaesthesiology, paediatrics and thoracic medicine intensive care units in YUH were cross-contaminated between 2009 and 2012 by ESBL-producing E. coli strains, which is a sign of nosocomial infection in YUH. Analysis of PFGE results gave rise to two main PFGE profiles, profile-A with four subprofiles and profile-B with three subprofiles, where profile-A predominates over profile-B (14%). Comparison of the antibiotic resistance profile with the PFGE profile yielded similarities while some differences also exist due to either identical restriction enzyme cutting sites with slightly different genetic sequences in between the cutting sites or newly formed restriction enzyme cutting sites that do not affect antibiotic resistance genes. Enterobacteriaceae, particularly E. coli, have developed resistance in YUH by producing ESBLs against oxyimino and non-oxyimino cephalosporins, and penicillin-type antibiotics. Therefore, more effective antibiotics such as cefoxitin or cefoperazone- sulbactam should be used for the treatment of future nosocomial infections in YUH while hospital staff should take care with hygiene, such as hand washing. © 2016, EDIMES Edizioni Medico Scientifiche. All rights reserved.
A Comparative Perspective on Brucellar, Pyogenic, and Tuberculous Spondylodiscitis
(Verduci Editore s.r.l, 2024) Baran, A.I.; Celik, M.; Arslan, Y.; Incecik, S.; Binici, I.; Toprak, M.; Sunnetcioglu, M.
OBJECTIVE: Non-specific features of spondylodiscitis lead to a delay and challenge in the diagnosis/differential diagnosis/treatment processes, and thus, serious complications may arise. This study aims to compare brucellar, pyogenic, and tuberculous types of spondylodiscitis, considering their demographic, clinical, and laboratory differences. This may provide more rapid management and good outcomes. PATIENTS AND METHODS: A total of 131 patients with infectious spondylodiscitis were included in the study. The patients were divided into brucellar (n=63), pyogenic (n=53), and tuberculous (n=15) types of spondylodiscitis and compared for demographic, clinical, laboratory, and imaging features. RESULTS: Tuberculous spondylodiscitis had higher scores for weight loss, painless palpation, thoracic spine involvement, and psoas abscess formation than other spondylodiscitis. Also, tuberculous spondylodiscitis had higher rates of neurologic deficit and lower rates of lumbar involvement than brucellar spondylodiscitis. Pyogenic spondylodiscitis is more likely to occur in patients who have a history of spine surgery compared to other forms of spondylodiscitis. Also, pyogenic spondylodiscitis had higher rates of fever, erythema, paraspinal abscess, white blood cell (WBC), and erythrocyte sedimentation rate (ESR) than brucellar spondylodiscitis. On the other hand, brucellar spondylodiscitis had higher rates of rural living and sweating than pyogenic spondylodiscitis. CONCLUSIONS: Weight loss, painless palpation, involved thoracic spine, psoas abscess, and neurologic deficit are symptoms favoring tuberculous spondylodiscitis. History of spine surgery, high fever, skin erythema, and paraspinal abscess are findings in favor of pyogenic spondylodiscitis. Rural living, sweating, and involved lumbar spine are symptoms that indicate brucellar spondylodiscitis. These symptoms can be used to distinguish the types of spondylodiscitis. © 2024 Verduci Editore s.r.l. All rights reserved.
Comparison of Carbapenem Resistance Detected by the Bd Phoenix Automated System in Enterobacteriaceae Isolates With E-Test Method
(Modestum LTD, 2022) Celik, M.; Sunnetcioglu, M.; Guducuoglu, H.; Arslan, Y.; Akyuz, S.; Baran, A.I.
Objective: Automatic identification and antimicrobial susceptibility systems are frequently used to identify clinical isolates in hospitalized patients, but mistakes in these systems can lead to potentially devastating treatment failures for patients. Therefore, the ‘‘Centers for Disease Control and Prevention (CDC)’’ recommends confirming all Carbapenem-resistant and low-susceptibility isolates with a different method. The aim of this study is to compare the Carbapenem susceptibility results of isolates reported as Carbapenem-resistant Enterobacteriaceae according to the BD Phoenix 100 automated system with the E-test method. Materials and Methods: The study included 70 strains of Carbapenem-resistant Enterobacteriaceae members which were isolated and grown from several types of clinical samples in the Medical Microbiology Laboratory. Conventional methods (Gram stain, negative oxidase test) and the BD Phoenix 100 automated system were used to identify the isolates. The susceptibility of all strains to imipenem, ertapenem and meropenem was investigated by E-test method. Automated system results and E-test results were compared. Results: The frequency distribution of all isolated bacterial strains comprised K. pneumoniae in 56 (80%) of the samples included in the study. The automated system test results were correlated with the results of the E-test at a rate of 96.1 % for the imipenem-resistant strains, 84.3% for the meropenem-resistant strains, 84.1% for the ertapenem-resistant strains Conclusions: Automated systems are frequently used in microbiology laboratories to identify isolates. However, automated systems can show a high error rate against some antimicrobials. For this reason, comparing the results of automated system test results with tests such as E-test is very important to prevent both treatment failures and inappropriate antibiotic use that may occur on a patient basis. © 2022 by Author/s and Licensed by Modestum.
Essential Trace Element Levels in Patients With Cutaneous Anthrax
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Binici, I.; Parlak, M.; Sunnetcioglu, M.; Baran, A.I.; Ceylan, M.R.; Kul, A.R.; Huyut, Z.
The causative microorganism in anthrax is Bacillus anthracis and this disease is more common in some regions of Türkiye. Changes in trace elements other than iron in anthrax infection have not been studied. In this study, iron, copper, lead, zi nc, manganese, magnesium, cadmium and cobalt levels were investigated in cases with cutaneous anthrax. Fifteen patients with cutaneous anthrax and 15 healthy individuals wereincluded in the study. The groups were similar to each other in terms of age and gender. Anthrax was diagnosed according to contact status with animals, symptoms, examination, and microbiological results. We performed our study with Atomic Absorption Spectrophotometer (UNICAM-929 spectrophotometer). Serum iron, lead, and cadmium levels were significantly higher in the patients than in the control subjects (p<0.05). Serum magnesium, manganese, zinc, copper, and cobalt levels were significantly lower in the patients than in the control subjects (p<0.05). The copper/zinc molar ratio was not significantly increased in the patients with cutaneous anthrax than in the control subjects. It has been reported that iron, cadmium, and lead levels are low and copper level is high in infectious diseases. But we determined the opposite situation in the patients with cutaneous anthrax. As a result, it can be said that the detection of high lead and cadmium levels in the cell in anthrax disease suppresses the immune system. Also, zinc can be used as a marker for this disease. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Evaluation of Oxidative Status in Patients With Fasciola Hepatica Infection
(Makerere Univ, Fac Med, 2011) Karsen, H.; Sunnetcioglu, M.; Ceylan, R. M.; Bayraktar, M.; Taskin, A.; Aksoy, N.; Erten, R.
Background: F. hepatica infection is rare and mostly subclinical. Migration of juvenile forms of F. hepatica into the host's liver is accompanied by an inflammatory reaction followed by fibrosis and cirrhosis. Objective: The aim of this study was to evaluate oxidative status by using a novel automated method in patients with Fasciola hepatica. Methods: Twenty two patients with a diagnosis of F. hepatica and 26 healthy volunteers were enrolled in the study. Their Total antioxidant capacity status (TAC), total oxidant status (TOS) and catalase were measured in them and oxidative stress index (OSI) was calculated. These measurements were also taken for the control group and the values were compared. Results: Plasma levels of total TOS and OSI were significantly increased in patients as compared with healthy controls (P<0.001, P<0.001, P=0.001, P=0.008) respectively. In contrast, TAC level was significantly lower in patients as compared with controls (p<0.05). There was no significant difference between the catalase results of the two groups (p>0.05). Conclusion: Total oxidative status and OSI were increased and total antioxidative status capacity was decreased in patients with F. hepatica infeciton. A high oxidative stress occurs during F. hepatica infection, which may cause severe damage in both the liver.
Rare Brucellosis Involvement: Thyroid Gland Abscess
(Yuzuncu Yil Universitesi Tip Fakultesi, 2015) Sunnetcioglu, M.; Ceylan, M.R.; Atmaca, M.; Baran, A.İ.; Mentes, O.; Ücler, R.
Brucellosis is a zoonotic disease, especially in endemic regions all over the world, it is a common infectious disease. However, brucellosis borne thyroid gland infection is quite rare. The purpose of this study is to draw attention to the thyroid abscess borne due to brucella, which also show an unusual clinical graphic. Within this study we aim to represent a case with thyroid abscess dominated Brucella spp. © 2015 Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Salmonella Ovarian Abscess in a Patient With Systemic Lupus Erythematosus Presented as Acute Abdomen: a Case Report and Review of the Literature
(Yuzuncu Yil Universitesi Tip Fakultesi, 2020) Baran, A.I.; Sunnetcioglu, M.; Yakan, U.; Batur, A.; Karagoz, E.; Karahocagil, M.K.
A Salmonella ovarian abscess is a rare entity, and is similarly uncommon in cases with Systemic Lupus Erythematosus. To the best of our knowledge, thirty four cases of Salmonella ovarian abscess have been published in the literature, and only four of them were related to Systemic Lupus Eritematosus. Our aim is to review this clinical entity using information from our own experience as well as that from published cases. © 2020, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Serum Adenosine Deaminase Activity in Cutaneous Anthrax
(International Scientific Literature Inc., 2014) Sunnetcioglu, M.; Karadas, S.; Aslan, M.; Ceylan, M.R.; Demir, H.; Oncu, M.R.; Aypak, C.
Background: Adenosine deaminase (ADA) activity has been discovered in several inflammatory conditions; however, there are no data associated with cutaneous anthrax. The aim of this study was to investigate serum ADA activity in patients with cutaneous anthrax. Material/Methods: Sixteen patients with cutaneous anthrax and 17 healthy controls were enrolled. We measured ADA activity; peripheral blood leukocyte, lymphocyte, neutrophil, and monocyte counts; erythrocyte sedimentation rate; and C reactive protein levels. Results: Serum ADA activity was significantly higher in patients with cutaneous anthrax than in the controls (p<0.001). A positive correlation was observed between ADA activity and lymphocyte counts (r=0.589, p=0.021) in the patient group. Conclusions: This study suggests that serum ADA could be used as a biochemical marker in cutaneous anthrax. © Med Sci Monit, 2014.
Serum Paraoxonase Activity and Oxidative Stress Levels in Patients With Cutaneous Anthrax
(Sage Publications Ltd, 2017) Karadas, S.; Aslan, M.; Ceylan, M. R.; Sunnetcioglu, M.; Bozan, N.; Kara, H.; Demir, H.
Introduction: Anthrax is a bacterial disease caused by the aerobic sporeforming bacterium Bacillus anthracis. It has been suggested that oxidative stress plays an important role in the pathogenesis of B. anthracis. The aim of this study was to investigate serum paraoxonase 1 (PON1) activity, catalase activity, malondialdehyde (MDA) levels, and superoxide dismutase (SOD) levels in patients with cutaneous anthrax. Materials and methods: Fifteen patients with cutaneous anthrax and 15 healthy controls were enrolled in this study. The serum MDA levels, SOD levels, paraoxonase, arylesterase, and catalase activities were measured using a spectrophotometer. Results: The serum SOD levels, paraoxonase, arylesterase, and catalase activities were significantly lower in patients with cutaneous anthrax than in controls (for all, p < 0.001), whereas MDA levels were significantly higher (p < 0.001). No significant correlation was found between serum paraoxonase activity, arylesterase activity, SOD levels, and MDA levels (all, p > 0.05) in patients with cutaneous anthrax. Conclusions: The current study was the first to show decreased antioxidant levels and increased oxidant levels in patients with cutaneous anthrax. Therefore, decreased PON1 activity may play a role in the pathogenesis of cutaneous anthrax.