Browsing by Author "Suzek, Huseyin"

Now showing 1 - 7 of 7

Effects of Subacute Exposure of Dichlorvos at Sublethal Dosages on Erythrocyte and Tissue Antioxidant Defense Systems and Lipid Peroxidation in Rats
(Academic Press inc Elsevier Science, 2009) Celik, Ismail; Suzek, Huseyin
In this study, the effects of dichlorvos (DIC) at sublethal concentration on malondialdehyde (MDA) content and antioxidant defense system (ADS) such as reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), and glutathione-S-transferase (GST) in various tissues of rats exposed to 0.0225 and 0.0450 millimole (mmol) DIC in drinking water were administered orally to rats ad libitum for 28 days continuously. MDA significantly increased in all the tissues except in the lungs of rats treated with both dosages of DIC. With regard to the ADS, GR significantly decreased in the erythrocytes whereas it did not change in the other tissues with two dosages of DIC. SOD activity significantly increased in the all tissues treated with both dosages of DIC. GST activity significantly increased in all the tissues except in the erythrocytes, spleen, and lungs treated with 0.0225mmol. Meanwhile, GSH depletion in the liver and heart of rats treated with 0.0225 mmol and all the tissues except in the brain and kidney of rats treated with 0.0450 mmol of DIC were found to be significant. The observations presented led us to conclude that the administrations of DIC promote MDA content and fluctuate in the ADS in rats during the exposure. (c) 2008 Elsevier Inc. All rights reserved.
Effects of Subacute Treatment of Ethylene Glycol on Serum Marker Enzymes and Erythrocyte and Tissue Antioxidant Defense Systems and Lipid Peroxidation in Rats
(Elsevier Ireland Ltd, 2007) Celik, Ismail; Suzek, Huseyin
The present study was aimed to investigate the effects of ethylene glycol (EG) on serum marker enzymes, antioxidant defense systems and lipid peroxidation concentration (malondialdehyde = MDA) in various tissues of rats exposed to ethylene glycol. EG (1.25% or 2.5%) in drinking water was administered orally to rats (Sprague-Dawley albino) ad libitum for 21 days continuously. EG treatments caused different effects on the serum marker enzymes, antioxidant defense system and MDA content in various tissues of the treatment groups as compared with the controls. EG also caused a significant increase in the serum marker enzyme activities with 2.5% dosage whereas, no changes were not observed with 1.25% dosage of EG treatment. Lipid peroxidation significantly increased in all the tissues except for in the heart and stomach of rats treated with both dosages of EG. Also, the antioxidative systems were also seriously affected by EG. For example, SOD significantly decreased in the liver treated with both dosages whereas, SOD activity in the erythrocytes, kidney, heart and stomach were significantly increased and not changed in the brain with two dosages of EG. Also, while CAT activity significantly decreased in the erythrocytes, liver and kidney, the activity in the stomach significantly increased, but did not change in the brain and heart with two doses of EG. GR activity significantly decreased in the erythrocytes treated with both dosages of EG whereas GR was not affected in other tissues by EG treatment. GST activity significantly elevated in the heart and brain but did not change in the other tissues of rats treated with both dosages of EG. Meanwhile, GSH depiction in the erythrocytes of rats treated with 2.5% dosage of EG was found to be significant whereas, the level of GSH in the brain was significantly increased treated with both the dosages of EG. The observations presented led us to conclude that the administration of subacute EG promotes lipid peroxidatin content, elevates tissue damage serum marker enzymes and changes in the antioxidative systems in rats. These data, along with the determined changes suggest that EG produced substantial systemic organ toxicity in the erythrocyte, liver, brain, heart kidney and stomach during the period of a 21-day subacute exposure. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
Effects of Subacute Treatment of Thiourea on Antioxidant and Immunopotential Marker Enzymes and Lipid Peroxidation of Rats: a Drinking Water Study
(Asian Journal of Chemistry, 2006) Celik, Ismail; Suzek, Huseyin; Tuluce, Yasin
The effect of subacute 25 days treatment of thiourea, an inhibitor of diene conjugate formation as hydroxyl radical scavengers were tested as terminators of lipid peroxidation (malondialdehyde = MDA), antioxidant [reduced glutathione (GSH), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT] and immunopotential [adenosine deaminase (ADA) and myeloperoxidase (MPO) defense systems marker enzymes in the liver tissue of rats under laboratory conditions. 250 ppm of thiourea was administered orally to 8 rats ad libitum during the tests for 25 days consecutively. Malondialdehyde content, antioxidant and immunopotential marker enzyme of liver were determined after treatment. According to results, while the treatment of thiourea caused significant decrease in MDA content, GSH level, GR activity and immunopotentical enzyme activities decreased in comparison to control rats. CAT, GPx, SOD and GST activities did not change significantly, nese results suggest that thiourea may not only reduce oxidative damage by induced oxygen reactive radicals in animals, but affect immunopotential enzymes too.
The Hematological Effects of Methyl Parathion in Rats
(Elsevier Science Bv, 2008) Celik, Ismail; Suzek, Huseyin
The effects of methyl parathion (MP) at sublethal concentration on hematological constituent [red blood corpuscles (RBC), white blood corpuscles (WBC), mean cell volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet (PLT) counts, hemoglobin (Hb) and hematocrite (HCT) levels] and serum damage marker enzymes [aspartate aminotransferase (AST), alanin aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH)l of rats were investigated under laboratory conditions. 5 and 10 ppm dosages of NIP were administered orally to six female rats ad libitum during the tests for 4 weeks consecutively. MP treatments caused different effects on the hematological constituents and the serum marker enzymes of the treatment groups as compared to the controls. According to the results, MP treatments increased significantly the levels of serum marker enzyme activities except for ALT with both dosages and LDH with 5 ppm dosage. Also, the hematological constituents were affected by MP. For example, WBC significantly increased in rats treated with both dosages of MP whereas the other hematologic constituents did not change at 5 and 10 ppm of MP treatments. The observations presented led us to conclude that the administration of subacute NIP elevates tissue damage serum marker enzymes, and increases the number of WBC in rats. These data, along with the determined changes suggest that MP produce substantial systemic organ toxicity in rats during the period of a 28-day subacute exposure. (C) 2007 Elsevier B.V. All rights reserved.
Nephroprotective Hepatoprotective Potential and Antioxidant Role of Carob Pods (Cerotonia Siliqua L.) Against Carbon Tetrachloride-Induced Toxicity in Rats
(Assoc Pharmaceutical Teachers india, 2017) Suzek, Huseyin; Celik, Ismail; Dogan, Abdulahad
Objective: The aims of current study are the evaluation of the protective effect and antioxidant role of carob pods against carbon tetrachloride (CCl4)-induced oxidative stress, hepatotoxicity and nephropathy. Material and Methods: The present experiment was designed as I (control), II (0.5 ml/kg CCl4), III (% 10 CP), IV (CCl4 0.5 ml/kg+% 10 CP) groups. While rats in group I and III were fed with a diet without CCl4, II, and IV groups received twice 0.5 ml/kg/week, where IV group additionally received % 10 CP supplementation for 50 days. The protective roles and antioxidant activity of the CP supplementation feed against CCl4-induced oxidative stress and toxicity were evaluated by histopathological changes, measuring hepatic and renal damage biomarkers (HRDB), antioxidant defence system constituents (ADSC) and malondialdehyde (MDA) parameters in the erythrocyte, liver, brain, kidney and spleen tissues of rats. Results: According to the results, the biochemical analysis showed a considerable increase in the serum AST (aspartate aminotransferase), ALT (alanin aminotransferase), GGT (gamma glutamyl transpeptidase) and LDH (lactate dehydrogenase) enzymes, creatinine and urea, and decrease in the group II as compared to that of I group. On contrary, such parameters were decreased in IV group as compared to that of group II. In addition, the results showed that CP supplementation diet restored the CCl4 induced MDA (malondialdehyde) and ADSC towards to control. The hepatoprotection of CP is further substantiated by the almost normal histological findings in IV group against degenerative changes in II group. Protective effects by CP are further substantiated by the almost normal HRDB for kidney and liver in IV treated group as against degenerative changes in the II treated rats. Conclusion: The results indicated that CP could be as an important as diet-derived antioxidants in preventing oxidative damage in the tissues by reducing the MDA or inhibiting the production of CCl4-induced free radicals and liver and kidney destruction.
Protective Effect and Antioxidant Role of Sweetgum (Liquidambar Orientalis) Oil Against Carbon Tetrachloride-Induced Hepatotoxicity and Oxidative Stress in Rats
(Taylor & Francis Ltd, 2016) Suzek, Huseyin; Celik, Ismail; Dogan, Abdulahad; Yildirim, Serkan
Context: Sweetgum oil (SO) obtained from the Liquidambar orientalis Mill (Hamamelidaceae) tree has been used in Turkish folk medicine for centuries as an antiulcerigenic. Some studies have reported the antibacterial and antioxidant activities of SO; however, its effect on hepatic and oxidative stress complications is still unexplored. Objective: This study investigates the hepatoprotective effect and the antioxidant role of SO against carbon tetrachloride (CCl4) toxicity. Materials and methods: The experiment included control, CCl4, SO, and CCl4+SO treatment groups. Control and SO group rats were fed a diet without CCl4. CCl4 and CCl4+SO treatment groups received 0.5mL/kg CCl4 diluted in olive oil (1:1 dilution) intraperitonally injection twice per week. The CCl4+SO group also received 1000mg/kg SO-supplemented feed for 50d. Blood and tissue samples were used for the determination of hepatic damage serum biomarkers (HDSBs) levels, antioxidant defense system constituents (ADSCs), and malondialdehyde (MDA) contents. In addition, the liver was evaluated for histopathological changes. Results: According to the results, the HDSBs levels of the CCl4 group were significantly (p<0.05) increased compared with the control, whereas the HDSB levels of the CCl4+SO group resulted in marked decreases (p<0.05) compared with the CCl4 group. In addition, the results showed that SO-supplemented diet restored the CCl4-induced MDA and ADS towards to control. Hepatoprotection of SO is further substantiated by the almost normal histologic findings in the CCl4+SO group against degenerative changes in the CCl4 group. Discussion and conclusion: It was concluded that SO has a hepatoprotective effect and antioxidant capacity against CCl4 toxicity.
Subacute Effects of Methyl Parathion on Antioxidant Defense Systems and Lipid Peroxidation in Rats
(Pergamon-elsevier Science Ltd, 2008) Celik, Ismail; Suzek, Huseyin
In this study we aimed to examine how methyl parathion (MP) at sublethal dosages affects on malondialdehyde (MDA) content and antioxidant defense system (ADS) such as reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD) and glutathione-S-transferase (GST) in various tissues of rats exposed to 19 and 38 millimole (mM) MP dosages as drinking water ad libitum for 28 days continuously. MDA significantly increased in all the tissues except for in the lungs of rats treated with both dosages of MP. With regard to the ADS, SOD significantly decreased in the lungs tissue whereas increased in the erythrocytes with two dosages of MP. GR activity significantly decreased in the erythrocytes treated with both dosages of MP, but decreased in the lungs and liver tissues with 38 mM MP treatment. GST activity significantly elevated in all the tissues except for in the liver treated with 38 mM dosage but did not change with 19 mM. Meanwhile, GSH depletion in all the tissues except lungs of rats treated with both dosages of MP was found to be significant. The observations presented led us to conclude that after the administrations of MP promote MDA content and fluctuate in the ADS in rats. (c) 2008 Elsevier Ltd. All rights reserved.