Browsing by Author "Tülüce, Y."

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Anticancer Effect of 1-(anthracen Chloride in 2d and 3d Cell Culture Models in Breast Cancer
(Yuzuncu Yil Universitesi Tip Fakultesi, 2024) Çakır, M.; Akar, S.; Çakır, Ş.; Akkoç, S.; Ozkol, H.; Tülüce, Y.
Triple negative breast cancer (TNBC), an aggressive subgroup of breast cancer that exhibits a highly complex and heterogeneous character has led researchers to seek for new and effective therapeutic agents due to the inadequacy of current treatment opt ions. In the literature, there are benzimidazole derivatives whose antiproliferative effects have been investigated in many in vivo and in vitro studies. In the present study, a benzimidazolium salt (BS), namely 1-(anthracen-10-ylmethyl)-3-(2-cyanobenzyl)-1H-benzo[d]imidazol-3-ium chloride was prepared from 2-((1H-benzo[d]imidazol-1-yl) methyl) benzonitrile (1 mmol) and 10-(chloromethyl) anthracene (1 mmol). In our study, it was aimed to investigate the anticarcinogenic effects of above-mentioned BS, which was previously shown to be more effective than cisplatin (the chemotherapy agent being used in the treatment of TNBC) on 4T1 cel ls. Cell viability 2-dimensional (2D) and 3-dimensional (3D), colony forming, 3D spheroid formation, wound healing scratch assay and Annexin V/Propidium Iodide (PI) staining analyzes were used to determine cell growth/proliferation, migration and apoptosis, respectively. According to the results, BS significantly reduced viability/proliferation, colony formation and migration in 4T1 cells, even at low doses. It also induced apoptosis. It was concluded that, BS is a potential antiproliferative agent that reduces the carcinogenic properties of 4T1 cells through inhibiting cell proliferation, migration as well as inducing apoptosis. Its mechanism of acti on should be elucidated with further studies. © 2024, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
The Apoptotic, Cytotoxic, and Anti-Migration Effects of Sodium Deoxycholate in a Breast Cancer Cell Line and Its Modulation on Pon1 as a Predictive Risk Marker
(Bentham Science Publishers, 2025) Tülüce, Y.; Alhammud, H.; Keleş, A.Y.; Köstekci, S.
Introduction: Breast cancer is the most prevalent cancer among women and is usually treated with antineoplastic drugs. The present study examines the influence of sodium deoxycholate on the molecular pathways underlying apoptosis, cytotoxicity, and the modulation of PON1 in the MCF-7 breast cancer cell line. Various doses were administered to test the hypothesis that it could potentially affect cancer cells. Method: The study examined the cytotoxic effect of sodium deoxycholate on MCF-7 cells and human mammary epithelial cells (CRL-4010) using the MTT method to detect its anticancer properties. Subsequently, the efficacy of the active dose on DNA fragmentation and apoptosis was examined using the apoptotic DNA ladder and Western blot methods. Additionally, oxidative stress index and cell migration tests were conducted. Notably, sodium deoxycholate did not cause DNA damage despite demonstrating cytotoxic effects on cells. Results: The study found that sodium deoxycholate increased the levels of several pro-apoptotic proteins, leading to apoptosis. Moreover, it markedly diminishes the activity of paraoxonase and arylesterase of PON1, which are predictive risk markers for cancer. Furthermore, it was found to delay cell migration in a time-dependent manner. Conclusion: These findings suggest that sodium deoxycholate exhibits an antimetastatic effect in breast cancer cells, could be a valuable subject for further cancer research. © 2024 Bentham Science Publishers.
Effects of Nigella Sativa L. on Lipid Peroxidation and Reduced Glutathione Levels in Erythrocytes of Broiler Chickens
(2009) Tülüce, Y.; Özkol, H.; Söǧüt, B.; Çelik, I.
We aimed to determine the effect of Nigella sativa L. on reduced glutathione (GSH) and lipid peroxidation (as malondialdehyde, MDA) in erythrocytes of broiler chickens. We used 100 Ross 308 chickens and they were equally divided into four groups namely control, 0.5 %, 1 % and 1.5 % containing Nigella sativa L. The control group received control broiler fattening feed whereas, the treatments groups were fed by the feed containing 0.5 %, 1 % and 1.5 % grinded Nigella sativa L. seeds for 6 weeks, respectively. Erythrocyte MDA levels were significantly lower in 0.5 % (p<0.002) and 1 % (p<0.005) of Nigella sativa L. groups than in control although GSH levels were significantly (p<0.005) higher in 0.5 % and 1 % of Nigella sativa L. groups. The 1.5 % of Nigella sativa L. did not induce on MDA and GSH levels in the animals. In conclusion, Nigella sativa L. caused protective effects on the oxidative stress-induced erythrocyte injury by inhibiting free radical production and regulation of GSH.