Browsing by Author "Tas, A."

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Analgesic Effect of Pimpinella Anisum L. Essential Oil Extract in Mice
(2009) Tas, A.
In this study analgesic effect of Pimpinella anisum essential oil extract were investigated in mice. In all the experiments mice were tested twice, 30 min before drug administration and 30, 90 and 150 min after drug administration by tail-flick device. Aspirin (100 mg/kg, peroral) and morphine hydrochloride (10 mg/kg, subcutan) were used as reference standard, it was determined that all of the study groups (except morphine group) had significantly analgesic effect when compared with control groups. There was no difference between aspirin and Pimpinella anisum essential oil groups. It was concluded that Pimpinella anisum essential oil had statistically higher significant analgesic effect similar to morphine and aspirin at the 90th min and with Aspirin at the 150 th min of the study.
Determination of Effect of Gibberellic Acid Treatments on the Fruit Quality of Strawberry Cv. Seascape
(Centenary University, 2017) Gundogdu, M.; Berk, S.; Canan, I.; Tuna Kocoglu, S.; Celik, F.; Tas, A.
In the study, the effect of gibberellic acid treatments on fruit quality of strawberry cv. Seascape grown in Bolu ecological conditions was examined. The fruit quality parameters were identified under the application of two concentration of GA3 (50 ppm and 100 ppm). It was determined that the values of SSC, pH, TA and fruit hardness were not statistically affected by both GA3 applications. The highest values related fruit weight and size were obtained at 50 ppm GA3 application. The SCC and firmness values were determined as 6.67 brix° and 1.31 kg/cm2 in 100 ppm concentration, respectively. The fruits treated with 50 ppm GA3 had lower pH than those treated 100 ppm GA3 and control. In addition, the highest fruit weight (46.01 g) and fruit acidity (1.11%) were obtained at the application of 50 ppm GA3. It was also found that the GA3 application did not cause a significant change in the flavor, taste and juice values. © 2017, Centenary University. All rights reserved.
Effect of Vitamin C Treatment on Biochemical Parameters in Acth Stressed Chickens
(2009) Cinar, A.; Beige, F.; Tas, A.
The study was conducted to examine the effects of vitamin C on plasma glucose, cholesterol, vitamin C and total protein in chickens stressed by adrenocorticotropin (ACTH). Three hours after ACTH application although the levels of glucose, cholesterol and total protein increased, the levels of vitamin C decreased with respect to mean initial values in control group after ACTH treatment (P < 0.05). It was concluded that intramuscularly administered vitamin C facilitated an adaptation against stress and decreased negative effects of stress in chickens.
The Effects of Peroneus Longus Trigger Poin Stimulation on the Pain Threshold in Dogs
(2010) Altug, M.E.; Tas, A.
The results of this study demonstrate that peroneus longus trigger point stimulation has a powerful analgesic effect, and increased algometric pain threshold without any adverse effect in the measured physiological parameters. Consequently, it may be suggested that only trigger point stimulation or its combination with the other analgesic or sedative drugs will be used effectively in alleviating painful conditions in dogs.
The Effects of Sildenafil Citrate and Vitamins A, C and E on Testicular Damage in Alloxan-Diabetic Rats
(Medwell Journals, 2012) Uslu, B.A.; Ilhan, F.; Gulyuz, F.; Karasu, A.; Tas, A.; Sendag, S.; Wehrend, A.
Sildenafil citrate is an active ingredient used successfully in the treatment of erection disorders caused by a variety of factors. The purpose of this study is to research the effects of Sildenafil citrate and vitamins A, C and E on testicular degeneration in rats with alloxan diabetes. The rats were divided into II groups; healthy control and diabetic groups. The diabetic rats were divided into X groups with ten animals in each group. Group I consisted of control diabetic rats that were given only distilled water, Group II was the diabetic group treated with glibenclamide; Group III was the diabetic group treated with insulin, Group IV was the diabetic group treated with Sildenafil citrate, Group V was the diabetic group treated with Vitamin A, Group VI was the diabetic group treated with vitamine, Group VII was the diabetic group treated with vitamin E, Group vni was the diabetic group treated with Sildenafil citrate and vitamin A, Group IX was the diabetic group treated with Sildenafil citrate and vitamin C and Group X was the diabetic group treated with Sildenafil citrate and vitamin E. Testis tissue samples were collected on the 3, 7 and 15th days of treatment in control and diabetic groups. In the histopathological examination of the testicles of diabetic rats, the seminiferous tubules and interstitium of testes in the control groups were normal and the complete spermatogenic cells in the seminiferous tubules were healthy and uniformly arranged. Severe degeneration and tubular atrophy was observed in the seminiferous tubules on the 3, 7 and 15th days in the group with induced alloxan diabetes given only distilled water (Group I). The lesions on day 3rd in the diabetic group given glibenclamide (Group II) were similar to those in the diabetic control group given only distilled water. All of the tubules had been affected by the 3rd day in group three which was given insulin. A small number of spermatogenic cells and giant cells were observed in some tubules. However, almost al of the degenerated tubules had healed on the 7 and 15th days. Even though, recovery on day 15th was very similar in Groups IV-X recovery was much more pronounced in Groups VIII-X beginning from day 3rd. © Medwell Journals, 2012.
Effects of Sildenafil Citrate on the Hematological Parameters in the Early Phase of Wound Healing in Diabetic Rats
(Academic Journals inc, 2011) Tas, A.; Karasu, A.; Comba, B.; Aksu, D. S.; Duz, E.; Tanritanir, P.
In this study Investigation of effects of sildenafil citrate on some of the hematological parameters in the early phase of wound healing in diabetic rats was aimed in this study. Total 30 rats were used in the study. Rats were divided into three groups equal in number (control-non diabetic, diabetic and diabetic+Sc). While no treatment was given to the control-non diabetic and diabetic groups, sildenafil citrate was given to the diabetic+Sc group intraperitoneally with a dosage of 0.7 mg/kg/day dosage for 3 days after creating wounds: under ketamine anesthesia, a circular, full thickness, standard wound was created on the back of each rat by using a 0.8 cm sterile punch. Cardiac blood was drawn in tubes containing EDTA from each group before the creation of wounds and on days 1, 2 and 3 following the creation of wounds. Differential leukocyte counts were determined in blood samples containing anticoagulant. For this purpose, blood smears were prepared from blood samples and stained with Giemsa method. It was found that, while neutrophil counts increased in all the groups during the wound-healing periods with the increasing number of days, lymphocyte counts reduced and in control and Sc+diabetic groups, monocyte counts increased. As a result, it was concluded that Sc plays an important role in eliminating the pressure on the cellular activities in the early period of wound healing and continuance of the inflammatory process and wound healing within normal limits and also that this could be demonstrated with hematological analyses.
Embolization After Internal Carotid Artery Injury Secondary To Transsphenoidal Pituitary Surgery and Delayed Intranasal Coil Protrusion: Illustrative Case
(American Association of Neurological Surgeons, 2022) Tas, A.; Bozan, N.; Akin, R.; Aycan, A.
BACKGROUND The authors presented a case of spontaneous nasopharyngeal coil migration that occurred 3 years after a patient had undergone transsphenoidal resection due to pituitary macroadenoma and was treated with coil application because of internal carotid artery injury secondary to transsphenoidal resection of the pituitary macroadenoma. OBSERVATIONS In the literature, eight cases of coil migration that occurred between 2 and 120 months after coil application have been reported, most of which were treated with surgical removal of the coil in a same-day surgery setting. LESSONS The case presented emphasized that coil protrusion and migration may lead to destruction in the skull base, thereby leading to serious consequences if left untreated, even in the absence of history of trauma. To the authors’ knowledge, this is the first case in the literature that required additional invasive procedures due to recurrent bleeding that occurred several months after surgical removal of coils. Also, this report underlinedthe need for careful and long-term follow-up of coil materials used for the treatment of pseudoaneurysms caused by vascular injuries secondary to skull base injury during surgery. © 2022 The authors.
Evaluation of a Mixture of Thiopental-Guafinesine and Sevoflurane Anesthesia in Horses
(Academic Journals inc, 2009) Atasoy, N.; Mercan, N.; Atalay, C.; Bayram, E.; Tas, A.
The anesthetic and cardiopulmonary effects of a combination of continuous intravenous infusion using a mixture of 6 g L-1 thiopental-75 g L-1 guafinesine-3 mg L-1 metedomidine (0.30 mL/kg/h) and Oxygen-Sevoflurane (OS) anesthesia (TGM-OS anesthesia) in horses were evaluated. The concentration of sevoflurane (Sevo) required maintaining surgical anesthesia was around 1.5% in TGM-OS and 3.3% in OS anesthesia. Mean Arterial Blood Pressure (MABP) was maintained at around 77 mm Hg under TGMOS anesthesia, while dobutamine (0.43 +/- 0.13 mu g kg(-1)) infusion was necessary to maintain MABP at 60 mmHg under OS anesthesia. No apparent complication was observed during and after anesthesia in all cases. Recovery from anesthesia tinder TGM-OS anesthesia was very calm and smooth. The times required for the horse to return both sternal and standing position in group under TGM-OS anesthesia tended to be shorter than group under OS anesthesia which statistical differences were p<0.05 and p<0.01, respectively. Thiopental Guafinesine-Metedomidine and Oxygen-Sevoflurane anesthesia (TGM-OS anesthesia) may be useful for prolonged equine anesthesia because of its minimal cardiopulmonary effects and good recovery from anesthesia.
Evaluation of Analgesic and Anti Inflammatory Activity of Pimpinella Anisum Fixed Oil Extract
(indian veterinary Journal, 2006) Tas, A.; Ozbek, H.; Atasoy, N.; Altug, M. Enes; Ceylan, E.
Pimpinella anisum is a member of the Umbelliferae and is found in the northeastern Anatolia (Zeybek, 1960). In Turkish folk medicine, Pimpinella species have several uses such. as analgesic, anti-inflammatory, appetizing, hypnotic, antispasmodic, expectorant,. antibacterial and hepato-protective. Seeds of P. anisum contains 1.5 - 2.0 % essential oil, 10-20 % fixed oil, 18 % protein. No reports are available on the evaluation of this plant for possible anti-inflammatory and analgesic activity: In this study, fixed oil of P. anisum was investigated for anti-inflammatory activity using carrageenan-induced rat paw oedema test (Winter et al., 1962) and analgesic activity with a tail-flick apparatus using a method initially described by D'amour and Smith (1941).
Histopathological and Immunohistochemical Study of the Effect of Sildenafil Citrate, Vitamin A, Vitamin C and Vitamin E on Wound Healing in Alloxan-Induced Diabetic Rats
(Univ West indies Faculty Medical Sciences, 2021) Tas, A.; Karasu, A.; Yener, Z.; Yildirim, S.; Atasoy, N.; Duz, E.; Yavuz, E.
Objective: To examine the impact of sildenafil citrate, vitamin A (Vit A), vitamin C (Vt C) and vitamin E (Vit E) on wound healing in a rat model of diabetes (n = 100). Methods: Diabetes was induced in the rats by intraperitoneal (i.p.) administration of alloxan. After anaesthesia, a standard wound was created on the back of each rat using a 10-mm sterile punch. The rats were randomly divided into 10 groups (n = 10 in each), as follows: normal saline, glibenclamide, insulin, sildenafil, Vit A, Vit C, Vit E, Vit A + sildenafil, Vit C + sildenafil, Vit E + sildenafil daily for 15 days. The rats were sacrificed after being anaesthetized 3, 7 and 15 days later Wounded skin tissue samples were collected for histopathological and immunohistochemical analyses. Results: On the 7th day, epithelial regeneration was completed in groups 8 and 9. Angiogenesis was insufficient in group 2. In terms of connective tissue proliferation, partially matured connective tissue was observed in group 4. On the 15th day of the study, groups 8, 9 and 10 had mature connective tissue. However; group 1 still had exudate-containing neutrophils. Immunohistochemically, on the 3rd day, the level of inducible nitric oxide synthase (iNOS) reactivity was intense in macrophages and neutrophils surrounding the wound edges in groups 4, 8, 9 and 10. The level of iNOS reactivity was moderate in group 6 and less distinct in groups 1, 2, 3, 5 and 7. Conclusion: Sildenafil citrate, together with Vit A and Vit C, is beneficial in wound healing of diabetic rats.
Histopathological Changes That Occur on the Testicular and Penile Tissues Depending on the Treatment of Human Chorionic Gonadotropin: Rat Model
(Univ West indies Faculty Medical Sciences, 2021) Pirincci, N.; Yildirim, S.; Tas, A.; Ozan, T.; Gecit, I; Ozveren, H.
Objective: To examine the histopathological effects of human chorionic gonadotropin (hCG) treatment on the penile and the testicular tissue in rat model. Methods: The rats of the hCG group (n = 8) were given daily subcutaneous injections of 50 IU of hCG for 15 days (Pregnyl, Organon). Rats of the control group (n = 8) received subcutaneous isotonic saline. All rats were sacrificed at the 1st month after hCG administration. After the received tissue samples were examined, germinal epithelial cell thickness, seminiferous tubule diameter; internal diameter of the tubules, the number of germ cell layers in the testicular tissue, and the diameters of penis, cavernous sinus lumen diameters and collagen tissue amount in the cavernous sinus surrounding were assessed in the sections prepared from the penis. Results: It was detected that there was a decrease in the testis weight, atrophy in the tubules, reduction in spermatogenesis, decrease in the mature spermatocytes, lower mean thickness and the number of cell layers of the germinal membrane in testicular tissue in the hCG group. It was found that the amount of collagen in the penile tissue was significantly higher in the hCG group and the diameters of cavernosal sinus lumens, and diameter of the penis were significantly lower in the hCG group. Conclusion: Human chorionic gonadotropin led to the deterioration in testicular histology and the histological changes in the penile tissue. The degradation in the testicular tissue and these changes formed in the penile tissue may affect the erectile tissue function.
Histopathological Effects of Desflurane on the Liver and the Kidney Using Light Microscopy
(2010) Kati, I.; Tekin, M.; Tas, A.; Ragbetli, C.; Ugras, S.
The aim of this study was to histopathologically assess the toxic effect of desflurane on the liver and the kidney. The rats (n = 150) were grouped into five main and three subgroups according to the exposure time to the anesthetic agent. The first group was planned as 1 MAC Desflurane+N2O+O2, the second group as 1 MAC Desflurane+O2, the third group as 2 MAC Desfiurane+N2O+O2, the fourth group as 2 MAC Desflurane+O2 and the 5th group as the control group. Each group was given the anesthetic agent for 1, 3 or 6 h. The rats that were exposed to the anesthetic agent with an adequate dose and for an adequate period of time were sacrificed properly. Livers and kidneys were resected and examined by light microscopes following the routine tissue follow-up procedures. Significant histopathological changes were observed in all groups compared to the control group. However, the pathological findings of the group 2 MAC Desflurane+N2O+O2 at the 6th h with the light microscope was significantly more than those of other groups at all times. It was concluded that although, desflurane caused cellular changes in liver and kidney tissue, it did not cause any clinical changes. Nevertheless, further studies are required to support this opinion. © Medwell Journals, 2010.
Investigation of Bdnf, Apoptosis and Cholinergic System Enzymes in Glioblastoma Multiforme Patient Serum
(Medical Project Poland, 2024) El-Tekreti, S.A.A.; Yeltekin, A.Ç.; Aycan, A.; Tas, A.
Background: Glioblastoma Multiforme (GBM) is the most common type of brain tumor and it is an aggressive form of adult brain cancer that is globally fatal. Unfortunately, the poor prognosis of patients with glioblastoma and current treatments do not appear to be very effective against glioblastoma as the disease is still fatal. Our study aimed to explore new ways of diagnosing the disease to develop a treatment for this deadly disease. Method: This study included 40 individuals (20 glioblastoma multiforme 20 control groups). The blood samples were taken from the study groups, and biochemical analysis was performed. The groups were then compared for statistical significance. Results: Our study aimed to determine the relationship between enzymes, some parameters levels and glioblastoma multiforme. When compared to the control group, the levels of GSH-Px, SOD, CAT, AChE, and BChE were decreased in patients with GBM, and the levels of MDA caspase-3, BDNF, TNF alfa, and 8-OHdG were increased. Conclusion: The results noted here point out that Apoptosis, Cholinergic System Enzymes, and BDNF are good and accurate markers in Glioblastoma Multiforme to know of diagnosing the disease. Will make it possible to use characteristics seen in glioblastoma multiforme as indicators for assessing tumor aggressiveness, as well as to get a better understanding of Proteins that are involved in apoptosis as well as anti-apoptotic proteins, allowing for the creation of novel molecularly based treatments. © Oncology and Radiotherapy.
Investigation of the Neuro-Regenerative Effects of Bioresonance and Magnetotherapy in Sciatic Nerve Damage-Induced Rats
(Universidad de la Frontera, 2023) Tas, A.; Nalci, K.A.; Sincan, S.; Özdemir, H.; Üstün, R.; Aras, I.; Tuncer, M.C.
Peripheral nerve damage is a significant clinical problem that can lead to severe complications in patients. Regarding the regeneration of peripheral nerves, it is crucial to use experimental animals' nerves and use different evaluation methods. Epineural or perineural suturing is the gold standard in treating sciatic nerve injury, but nerve repair is often unsuccessful. This study aimed to investigate the neuroregenerative effects of magnetotherapy and bioresonance in experimental animals with sciatic nerve damage. In this study, 24 female Wistar rats were divided into 7 groups (n=6) as follows: Group 1 (Control), Group 2 (Axonotmesis control), Group 3 (Anastomosis control), Group 4 (Axonotmesis + magnetotherapy), Group 5 (Anastomosis + magnetotherapy), Group 6 (Axonotmesis + bioresonance), Group 7 (Anastomosis + bioresonance). Magnetotherapy and bioresonance treatments were applied for 12 weeks. Behavioural tests and EMG tests were performed at the end of the 12th week. Then the rats were sacrificed, and a histopathological evaluation was made. The statistical significance level was taken as 5% in the calculations, and the SPSS (IBM SPSS for Windows, ver.21) statistical package program was used for the calculations. Statistically significant results were obtained in animal behaviour tests, EMG, and pathology groups treated with magnetotherapy. There was no statistically significant difference in the groups treated with bioresonance treatment compared to the control groups. Muscle activity and nerve repair occurred in experimental animals with acute peripheral nerve damage due to 12 weeks of magnetotherapy, and further studies should support these results. © 2023, Universidad de la Frontera. All rights reserved.
Oxidative Stress Status in Patients With Lumbar Disc Herniation
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Tas, A.; Demir, C.
Lumbar disc herniation (LDH) is a degeneration process that causes nerve damage with mechanical and inflammatory effects. Data on oxidative stress’s role in disease pathogenesis and prognosis are minimal. In this study, we analyzed oxidative stress parameters in LDH patients. The study included 63 patients who were diagnosed with LDH by magnetic resonance imaging and decided to operate and the same number of healthy controls. Oxidative stress parameters were measured from the patients preoperatively and i n the sixth postoperative month. The obtained values were compared among themselves and with the control group. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSHPx) levels were found to be significantly lower in the preoperative period compared to the control group, and postoperative period, meanwhile malondialdehyde (MDA) was higher (p=0.001). When postoperative patients were compared with the control group, it was revealed that SOD, CAT, and GSHPx were lower, and MDA was higher than the control group (p=0.001). In this study, it was shown that surgery significantly improved the oxidative stress state in patients with LDH but could not eliminate it. In other words, oxidative stress and cellular damage continue at the molecular leve l. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Prophylactic Effects of Silver Nanoparticles in a Rat Model of Spinal Stabilization and Infection
(Yuzuncu Yil Universitesi Tip Fakultesi, 2022) Tas, A.; Guzey, F.K.; Tufan, A.; Kuvat, N.; Aktaş, Ö.Y.; Yücel, M.; Akkalp, A.K.
Several factors increase the risk of postoperative infection. Some experimental studies have shown that silver nanoparticles (AgNP) have a protective effect from surgical infection. This study aimed to determine the effectiveness of AgNP in preventing the development of infection in the spinal stabilization rat model. In this study, 32 Sprague-Dawley rats weighing 300–350 g were used. Subjects were randomly divided into four groups. Titanium screws were placed in the spine in all groups. In Groups 2, 3, and 4, S.aureus and P.aeruginosa were cultivated in the surgical site at a concentration of 106 CFU/10 μL. Additionally, 0.5 mg/kg AgNP in maltose solution in Group 3 and maltose solution in Group 4 were used. The histopathological examinations were then done. In the fascia, muscle, bone, and screws in all groups, except in Group 1 (control group), S. aureus and P.aeruginosa reproduced. In blood cultures, there was growth of S. aureus and P. aeruginosa in 1 subject in Group 2, 6 subjects in Group 3, and 2 subjects in Group 4. The growth of S. aureus and P.aeruginosa was significantly i ncreased in Group 3. The degree of inflammation was significantly lower in Group 1. No accumulation of silver was detected in any organs histopathologically. Application of 1 eucal AgNP decreased the severity of infection. This dose was not toxic. However, administration of AgNP at this dose did not decrease the local infection rate. Therefore, new studies should be conducted to determine the ideal effective dose. © 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Treatment and Outcomes of Patients With Metastatic Spinal Cord Compression: a Double-Center Study
(NLM (Medline), 2023) Aycan, A.; Eren, B.; Tas, A.; Celik, S.; Karagoz Guzey, F.; Kuyumcu, F.; Aycan, N.
OBJECTIVE: Spinal metastases may only affect the bone tissue and result in spinal instability or may additionally result in epidural compression, leading to neurological deficits. Surgery has emerged as a popular method in treating metastatic epidural spinal cord compression (MESCC) due to the advances in surgical techniques and instrumentation. In this study, we evaluated patients with MESCC regarding neurological status, pain status, and survival rates, and presented our experience managing MESCC. PATIENTS AND METHODS: Clinical and radiographic records of 53 patients diagnosed with MESCC between January 2011 and March 2017 were retrospectively evaluated. The study included patients with a pathological diagnosis of primary cancer, those who complained of spinal metastasis, and those who had indications of MESCC on Magnetic Resonance Imaging (MRI). Bone structure and spinal stability were evaluated using assessed Computed Tomography (CT), and metastatic spread was considered using assessed Positron Emission Tomography (PET) in suitable cases. For each patient, the presence of a tumor compressing the spinal cord, age, gender, preoperative, and postoperative American Spinal Injury Association scores (ASIA), Tokuhashi prognostic score (TPS), affected spinal segment, pathological diagnosis, preoperative, and postoperative Visual Analog Scale (VAS), the status of spinal stability, follow-up period, and complications were evaluated. RESULTS: Forty-five patients (82.2% of them were women) underwent surgery with a mean age of 58.29 ± 15.14 years. The most frequent type of primary tumor was multiple myeloma (33.9%), followed by lung (24.6%), gastric (7.5%), and prostate (5.7%). The most common site of metastasis was the thoracic region (43.4%), followed by lumbar (24.5%), multiple (24.5%), and cervical (5.7%). The analysis indicated that a significant difference was found between the survival rates of the TPS categories. CONCLUSIONS: Common symptoms of MESCC include spinal pain and neurological deficit below the level of the injury. Prompt surgical treatment followed by oncological treatment leads to significant neurological recovery, more prolonged survival, pain relief, and improved quality of life in patients with a short survival time. Oncological treatments, including radiotherapy (RT), should be recommended after surgical treatment.