Browsing by Author "Tasal, I"

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Comparison of Fibrin Glue and Suture in the Healing of Teat Incisions in Lactating Goats
(M H Schaper Gmbh Co Kg, 2008) Alan, M.; Yener, Z.; Tasal, I; Bakir, B.
The aims of this study were to investigate whether fibrin glue can be used to close experimentally induced incisions of the teat (mammary papillae) in lactating goats and to compare the healing of the glued with the sutured incisions. Four clinically healthy lactating dairy goats, namely 8 mammary papillae were used. After surgical preparation of the papillae, a 3.5 cm long incision of each papilla was made through skin, muscular layer and mucosa into the papillary sinus. The wounds in the right papillae in all goats were closed with U-shaped uninterrupted 00 chromic catgut sutures. The wounds in the left papillae in all goats were closed, using fibrin glue. One incision was seen to be dehisced and fistulous one day after in fibrin glued teats. The animals were slaughtered 8 days after surgical manipulation. The mammary papillae were removed and examined in the viewpoint on gross and microscopic findings. The healing of wounds was slower and feeble in glued mammary papillary incisions, however faster and stronger in sutured incisions on day 8 after operations. But, available outcomes like less tissue thickness and positive cosmetic results could be obtained by fibrin glue used on mammary papillary incisions, which are very important for teats to be milked by hand and milking machine. Results suggest that it is advisable to use only one or two simple interrupted sutures in teat incisions glued with fibrin to prevent the dehiscence but with a more reliable healing than the sutured incisions.
The Effect of Follicle Diameter on Estrous and Ovulation Time During Pgf2α Injection in Mares
(Scientific Technical Research Council Turkey, 1998) Semacan, A; Demirel, M; Tasal, I
Variations in estrous and ovulation times occur because of follicle diameter during PGF2 alpha injection, in mares. The purpose of this study was to see the effect of follicle diameter on estrous and ovulation. Therefore 43 estrous cycles were evaluated in 32 Thoroughbred mares. Ten mg PGF2 alpha (Dinoprost Tromethamine, Dinolytic) was injected intramuscularly in diestrous period for synchronization. Follicle diameters were measured and divided into groups in centimeters as follows less than or equal to 1 cm, 1-2 cm, 2-3 cm, 3-4 cm ve > 4 cm, during PGF2 alpha injection. Result of daily teasing and rectal palpation every other day and daily rectal palpation during estrous period revealed that post injection-estrous. estrous period, post injection-ovulation. and estrous-ovulation times were in group less than or equal to 1 cm 5.91+/-2.71, 7.25+/-1.43. 11.75+/-2.23 and 6.16+/-1.95 days, in group 1-2 cm 4.81+/-2.84, 6.56+/-1.94, 10.68+/-2.95 and 5.87+/-2.85 days, in group 2-3 cm 3.90+/-1.44, 5.10+/-1.57, 8.00+/-1.78 ad 4.20+/-1.53 days and in group 3-4 cm 3.00+/-1.41, 4.60+/-1.35. 6.20+/-2.31 and 3.60+/-1.01 days, respectively. There was no follicle > 4 cm during PGF2 alpha injection. As a result, reduced estrous and ovulation times occured because of enlarged follicle diameters during PGE2 alpha injection. Therefore it was concluded that evaluation of ovaries for follicle structurs before PGF2 alpha injection would be beneficial.
Effects of a Gnrh Agonist on Oocyte Number and Maturation in Mice Superovulated With Ecg and Hcg
(Elsevier Science inc, 2004) Kanter, M; Yildiz, C; Meral, I; Koc, A; Tasal, I
The objective was to investigate the effects of a gonadotropin-releasing hormone agonist (GnRH) on ovulation rate and the number and maturation of oocytes in mice superovulated with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG). Thirty 3-month-old BALB/C female mice (weight: 25-30 g) were assigned to three experimental groups: control, superovulated, and superovulated with GnRH pretreatment (n = 10 per group). Control mice received an i.p. injection of 0.1 ml physiological saline solution. Superovulation was induced with 5 IU eCG (i.p.) and 5 IU hCG 48 It later. Mice in the superovulated with GnRH pretreatment group were given GnRH (20 mg/kg Fertirelin, i.m.), 24 h before superovulation. Thirteen hours after hCG administration, mice were sacrificed by cervical dislocation and blood samples were collected to determine serum progesterone concentration (by radioimmunoassay). Ovaries and oviducts were also harvested to enumerate corpora lutea and cumulus-enclosed oocytes. Progesterone concentrations were not significantly different among groups. The oocyte number and the maturation, ovulation rate, and the number of corpora lutea were higher in GnRH-treated mice than both controls and superovulated mice. In conclusion, GnRH given 24 h before superovulation with eCG-hCG increased the number and maturation of oocytes and the rate of ovulation in mice. (C) 2003 Elsevier Inc. All rights reserved.
The Effects of Prednisolone and Serum Malondialdehyde Levels in Puppies With Experimentally Induced Meconium Aspiration Syndrome
(Sage Publications Ltd, 2003) Kirimi, E; Tuncer, O; Kösem, M; Ceylan, E; Tas, A; Tasal, I; Caksen, H
The aim of this study was to investigate the effect of different doses of prednisolone in puppies experimentally induced with meconium aspiration syndrome (MAS). Meconium was collected from human babies in the first day of life and was released into the trachea of 11 newborn puppies to induce MAS. Puppies were treated with 2 mg/kg prednisolone (standard dose), 30 mg/kg prednisolone (megadose) or 0.9% saline, all administered intravenously. The study ended 20 h after meconium aspiration and the lungs were then scored for histopathology. Animals not treated with prednisolone deteriorated after 8 h while respiration rate, oxygenation, pH and partial pressure of carbon dioxide values were better in the prednisolone-treated groups. Histopathology scores were better in the treatment groups compared with the control group, with megadose giving the best result. At the end of the study, serum malondialdehyde levels were significantly higher in the megadose prednisolone group compared with the other two groups. In conclusion, we determined that prednisolone reduced physiological and histological changes in puppies with MAS and that a 30 mg/kg dose was more effective than 2 mg/kg.
Efficacy of Prostaglandin F2α and Misoprostol in the Induction of Parturition in Goats
(British veterinary Assoc, 2002) Alan, M; Tasal, I
Estimation of Early Pregnancy by Electrical Resistance Values of Vaginal Mucosa in Cows and Heifers
(Ecole Nationale veterinaire Toulouse, 2005) Tasal, I; Ataman, MB; Aksoy, M; Kaya, A; Karaca, F; Tekeli, T
The aim of this study was to investigate the availability of vaginal resistance values to estimate early pregnancy in heifers and cows on days 18-21 post-insemination. Seven Brown Swiss cows and ten heifers without any palpable reproductive disorders were used. Cloprostenol, a synthetic analogue of PGF(2 alpha), was administered intramuscularly twice (500 mu g) 11-days apart. After second cloprostenol administration, animals were observed for the overt signs oestrus and, inseminated twice with frozen semen after confirmation of oestrus by rectal palpation. Electrical conductivity of vaginal mucosa was measured through ventral wall of vagina adjacent to caudal end of cervix uteri by a handy-held instrument. Vaginal conductance of animals was measured twice, with a 12 h interval, on days 0 (day of oestrus), 3, 6, 10, 11 19 20 and 21 in cows, and on days 0 (day of oestrus), 3, 6, 10, 13, 18, 19 and 20 in heifers. To estimate plasma progesterone levels, jugular blood samples (10 mL) were also collected on days 19, 20 and 21 for cows, and 18, 19 and 20 for heifers. Pregnant animals were detected by rectal palpation 60 days after insemination. In pregnant cows, electrical resistance of vaginal mucosa measured between days 19-21 was significantly higher than non-pregnant animals, while vaginal resistance values determined on the day of insemination, and on days 3, 6, 10 and 13 post-insemination were similar in both groups. Likewise, vaginal impedance readings in pregnant heifers determined between days 18-20 were significantly higher than those non-pregnant animals. Vaginal resistance measurements in cows (between days 19-21) and heifers (between days 18-20) were significantly correlated with plasma progesterone concentrations both in pregnant and non-pregnant groups. It was concluded that vaginal resistance values might be used for the diagnosis of early pregnancy as a contributor or predictor method in cows and heifers.
Estrous, Ovulation and Pregnancy Rates Following Pgf2α Injections in Mares
(Scientific Technical Research Council Turkey, 1998) Demirel, M; Semacan, A; Tasal, I
Prostaglandin F2 alpha was commonly used for synchronisation in mares. The purpose of this study was to see the effect of single and double doses of PGF2 alpha on estrous, ovulation and pregnancy rates. Therefore 43 Thoroughbred mares, between 4-17 years old, were used. Thirty mares 10 mg PGF2 alpha (Dinoprost Tromethamine, Dinolytic) was injected intramuscularly, regardless of the stage of the estrous cycle, for synchronization. Estrous behaviors and follicular development were examined with daily teasing and rectal palpation every other day, after PGF2 alpha injection and ovulation times determined daily rectal palpation during estrous. The mares which in estrous (n: 11) natural service or artificial insemination with fresh semen were done every other day until estrous behavior lasted. The mares (n: 19), were not estrous after first PGF2 alpha injection, were injected second PCF2 alpha 14 days later and repeated all applications after first PGF2 alpha injections. Control group (n: 13) were chosen from the mares which were not injected PGF2 alpha in estrous and natural service or artificial insemination with fresh semen were done every other day until estrous behavior lasted. For pregnancy diagnosis, rectal palpation were done all mares between 30-35th and 60th days after ovulation. Estrous, ovulation and pregnancy rates obtained from first or second PCF2a injected mares and control group were 36.66%, 33.33% and 45.45%; 68.42%, 78.94% and 46.66; 100%, 92.30% and 53.84%, respectively. Consequently, because of no detrimental effect of PGF2 alpha injections on estrous, ovulation and pregnancy rates, it was concluded that PGF2 alpha injections for synchronization but, estrous and ovulation rates low in single dose injection therefore double doses injections would be more beneficial.
Venous Blood Gases, Ph and Bicarbonate Values in Oestrus-Synchronised Cows
(British veterinary Assoc, 2005) Tasal, I; Ceylan, E; Keles, I; Gülyüz, F