Browsing by Author "Tasal, Ibrahim"

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Concentrations of Nefa, Β-Hba, Triglycerides, and Certain Blood Metabolites in Healthy Colored Angora Goats During the Peripartum Period
(Tubitak Scientific & Technological Research Council Turkey, 2015) Eski, Funda; Tasal, Ibrahim; Karsli, Mehmet Akif; Sendag, Sait; Uslu, Baris Atalay; Wagner, Henrik; Wehrend, Axel
The aim of this study was to determine the changes in serum nonesterified fatty acid (NEFA), serum beta-hydroxybutyric acid (beta-HBA), triglycerides, Ca, Na, and other metabolites (bilirubin, glutamate dehydrogenase (GLDH)) in the blood of grazing, healthy goats at the time of parturition. Blood samples were taken weekly from the jugular vein of 11 goats, starting at week 2 antepartum (ap) until week 9 postpartum (pp). NEFA and beta-HBA concentrations increased from week 2 ap to 2 weeks pp. The increase in NEFA level was not significant; however, the beta-HBA levels were higher (P < 0.05) 2 weeks pp compared to the levels at 2 weeks ap. Triglycerides were recorded at maximum levels (P < 0.05) 2 weeks ap, with the lowest concentrations at 3 weeks pp. Bilirubin levels consistently increased up to 7 weeks pp, followed by a decrease. However, these changes were not significant. Similarly, GLDH activities increased until week 8 pp. A significant difference (P < 0.05) was recorded between the 1st week and 8th week pp. Ca and Na levels were lower during the 1st week pp and increased at 3 weeks pp. The results show that there are characteristic alterations of some metabolic blood parameters in goats around the time of parturition, which may be related to physiological changes.
Effects of Melatonin on the Onset of Ovarian Activity in Turkish Van Cats
(Medwell online, 2009) Gulyuz, Fetih; Tasal, Ibrahim; Kaya, Mustafa; Uslu, Baris Atalay
With the present study, we aimed to investigate the effects of melatonin on the initiation of breeding season in the female cats. For this purpose, 12 female cats and 2 tom-cats (male cats) (vasectomized) at age of 2-6 years old were used. The female cats were randomly divided into control (group 1, n = 6) and melatonin-treated groups (group 2, n = 6). Sham (placebo) and melatonin implants (containing 18 mg of melatonin) were placed subcutaneously into the females during the late anoestrous in groups 1 and 2, respectively. Sexual behaviors, vaginal smears and serum progesterone levels for the animals were recorded. The effect of the daylight (varying from 9.5-12.5 h, December through April) on the cyclic activity were also assessed during the study period. The animals were sexually silent during the anoestrus period. Upon the commencement of breeding season, while the female cats in group I showed estrus signs, none but one of the female cats in group 2 possessed the indications of the estrus. Moreover, the findings of cytology were not statistically different in both groups during the study period (p >= 0.05). Although, the progesterone levels in breeding season were higher in group 1 (p <= 0.05) than in un-breeding season, no such difference was present in group 2. The progesterone levels in breeding season were markedly elevated (p <= 0.01) in group I with comparison to group 2. In the latter group, melatonin effectively postponed cyclic activity of the females. The present results suggest that the administration of exogenous melatonin might prolong un-breeding season, presumably via the suppression of hypothalamic centers. Besides, we found that exposure to daylight of 10.5 or more hours per day were also, sufficient for the onset of cyclic activity in the female cats.
Effects of Oestrus Synchronisation Using Melatonin and Norgestomet Implants Followed by Ecg Injection Upon Reproductive Traits of Fat-Tailed Morkaraman Ewes During Suckling, Anoestrus Season
(Elsevier, 2012) Uslu, Baris Atalay; Tasal, Ibrahim; Gulyuz, Fetih; Sendag, Sait; Ucar, Omer; Goericke-Pesch, Sandra; Wehrend, Axel
The aim of this study was to compare the efficacy of different oestrus synchronisation protocols using melatonin (MEL), norgestomet (NOR) and melatonin + norgestomet (MELNOR) treatments (further, all animals from the groups MEL, NOR and MELNOR were supplemented with equine chorionic gonadotropin, eCG) in fat-tailed Morkaraman ewes during the non-breeding season. Forty healthy suckling ewes were assigned, from mid-May onwards, randomly to one of four experimental groups. Ten ewes were assigned to the control group CON (n = 10) and received no treatments. Ten ewes were assigned to the MEL group (n = 10) receiving a subcutaneous melatonin ear implant for 35 days, followed by a 500 IU intramuscular (i.m.) injection of eCG. Ten ewes were assigned to the NOR group (n = 10) and received norgestomet implants placed subcutaneously in the ear for 10 days and followed by a 500 IU i.m. injections of eCG after removal of the implant. The last 10 ewes were assigned to the MELNOR group (n = 10) receiving a subcutaneous melatonin ear implant for 35 days plus a subcutaneous norgestomet ear implant, inserted on the 25th day of the melatonin implant, for 10 days. It was then followed by a 500 IU i.m. injection of eCG after removal of the norgestomet implant and by introduction of rams to the flock concurrently. Ewes exhibiting oestrous signs by 7 days were hand-mated with rams known to be fertile. Blood samples were collected at the time of first implantation (baseline), on day 35 (norgestomet implant removal and eCG injection) and when ewes expressed oestrus in order to determine plasma progesterone (P-4) concentrations. The values of oestrus response, onset of oestrus post-treatment, pregnancy/lambing rates and litter sizes were recorded. The results showed that the oestrus rates were identical for all synchronisation protocols (100%) and the oestrus rates (40 +/- 16.3%) were significantly lower (P < 0.001) in the control group. The onset of oestrus shortened slightly in the hormone-treated groups. The P-4 concentrations on the day of eCG injections (at the end of synchronisation) were significantly higher (P < 0.01) in the hormone-treated groups (varying from 2.45 to 2.91 ng/ml) compared to the control group (0.45 ng/ml). Pregnancy/lambing rates were slightly higher in the synchronised groups (varied from 60 to 80%), compared to the control group. The results of oestrus synchronisation with melatonin and norgestomet ear implants (supplemented with the eCG) showed that: (i) an oestrus onset could be shortened significantly (P < 0.001) with melatonin and/or norgestomet, and (ii) significantly higher (P > 0.01) P-4 concentrations and higher lambing rates could be obtained in all hormone-treated ewes when compared to the un-treated Morkaraman ewes during the suckling (anoestrus) season. (C) 2012 Elsevier B.V. All rights reserved.
Electroejaculation in Impotent Rams and Artificial Insemination in Ewes
(indian veterinary Journal, 2006) Kaya, Mustafa; Gulyuz, Fetih; Uslu, Baris Atalay; Tasal, Ibrahim
This research was carried out to evaluate the efficiency of semen collection by the electroejaculation, method from Kivircik impotent rams and Al using fresh semen in Akkaraman ewes.
Investigation of the Immunocastration Efficacy and Longevity of a Single Dose Gnrh Vaccine in Young Male Rats
(Parlar Scientific Publications (p S P), 2019) Eski, Funda; Mis, Leyla; Tasal, Ibrahim; Uslu, Baris Atalay; Comba, Bahat
Conventionally, immunization protocol require an initial priming immunization followed by an antigen boost for adequate immunity and long-term immunization. In this study we were evaluate the immunocastration efficacy and longevity of immunization with a single-dose GnRH vaccine (Repro-Bloc (TM)) in young male rats. Rats in the vaccine group (VAC, n: 35) were treated with GnRH vaccine as single-dose (200 mu l) and the control group (C, n: 15) were also injected with saline solution. Blood samples from all rats were taken monthly for 6 months and at 12th month. GnRH antibody titers, testosterone and leptin levels were determined by commercial RIA kits. Rats at 6th and 12th months were sacrificed for histologic analysis of testes. GnRH antibody levels were found similar in the VAC and C groups (P>0.05) at -1 week. As compared to the C, GnRH antibody levels (pg/ml) of the VAC group at 1st, 2nd, 3rd and 4th months rose continuously and reached the peak level at 5th month, decreased at 6th month and increased again by the 12th month (P<0.05), whereas in the C group GnRH antibodies weren't different (P>0.05). Compared to the unchanged testosterone levels within the process in C group, serum testosterone levels of the VAC group were lower at 2nd month and higher at 3th month, and thereafter lower (P<0.05). Leptin levels were no difference between groups (P>0.05). There were significant differences in the testicle weights, sperm motility, sperm numbers and abnormal sperm rates between VAC and C groups at 6th and 12th months (P< 0.05). Histological examination showed no significant difference in testes between groups at 6th and 12th months (P>0.05). In conclusion, although the vaccine reduced the germinative and endocrine testicular function from 6th months to 12th months with the single-dose GnRH vaccine, the single-dose vaccination did not provide adequate effect in the genital organs in male rats. Further studies are needed to determine an optimal single-dose, longevity of the vaccine and route of administration for complete suppression on the genital organs in different animal species.
Investigation of the Seroprevalance of Maedi-Visna in the Region of Van Using Elisa and Histopathological Findings
(Medwell online, 2009) Akkan, Hasan Altan; Karaca, Mehmet; Tutuncu, Mehmet; Keles, Ihsan; Ilhan, Fatma; Cetin, Yunus; Tasal, Ibrahim
In the present study, seroprevalance of maedi-visna in sheep in the region of Van was aimed to investigate. A total of 465 serum samples obtained from sheep raised in Van and its towns were investigated with regard to maedi-visna specific antibody using ELISA procedure. Out of 465 samples 30 of them were seropositive (6.45%) and 20 of them were doubtful (4.30%) according to test procedure. Histopathology was also applied to some of these sheep which slaughtered in an abattoir after blood sampling. According to histopathological analysis 9 doubtful samples were also found to be positive. But, due to mild lymphoproliferation in there samples ELISA gave doubtful results. As a result with this study, seroprevalance of maedi-visna was determined in the region of Van and in the doubtful cases histopathological assessments believed to strengthen serological findings.
Is There a Relationship Between Serum Minerals (Ca, Mg) and Trace Elements (Cu, Fe, Mn, Zn) at Mating on Pregnancy Rates in Fat-Tailed Morkaraman Sheep
(Agricultural Research Communication Centre, 2017) Uslu, Baris Atalay; Mis, Leyla; Gulyuz, Fetih; Comba, Bahat; Ucar, Omer; Tasal, Ibrahim; Wehrend, Axel
The specific roles of nutrients in reproduction are not well defined in sheep. Hence, the relationships between certain mineral (Ca, Mg) and element (Cu, Fe, Mn, Zn) levels at mating and pregnancy rates was investigated in fat-tailed Morkaraman ewes synchronised with different hormones (melatonin, norgestomet implant and eCG) early in anoestrus season. For this purpose, 40 healthy ewes (aged 2-4 years) allocated in the synchronised (n=30) and no-treated group (n=10) were used during mid-May (suckling period). On the eCG injection day, fertile rams (n=8) were introduced into the groups and, then the oestrus signs were visually monitored twice daily for 7 days. The oestrous ewes were allowed to hand-mate using rotational (four rams daily) mating system. Additionally, jugular blood samples were collected on the days of onset of oestrus (before mating) for determination of serum Ca, Cu, Fe, Mg, Mn and Zn levels. Presence of pregnancies was diagnosed by real-time ultrasonography 35 days post-mating. Totally, 25 ewes became pregnant (21 ewes with stimulated-and 4 with spontaneous oestrus), while 9 ewes (all received stimulation) were not pregnant. In non-pregnant ewes, unlike other minerals and elements, the Cu and Zn values were significantly lower than their reference values (for Cu: 0.87 +/- 10.16 mg/dl vs. 9.20 mg/dl; for Zn: 2.06 +/- 10.30 mu g/dl vs. 3.8 mu g/dl, resp.; P<0.01 in both cases). In pregnant ewes, the corresponding values remained unchanged (P>0.05). Hence, the present findings indicate that the low Cu and Zn levels during the oestrus might adversely affect the subsequent pregnancy rate in suckling ewes.
Pregnancy Diagnosis in Ewes by Real-Time Ultrasound
(indian veterinary Journal, 2006) Tasal, Ibrahim; Dinc, D. Ali; Erdem, Huseyin; Semacan, Ahmet
The optimum time for diagnosis of pregnancy using B-mode real-time ultrasound transabdominally in different stages of gestation was studied in 1300 ewes. The ultrasonic results were compared with lambing records, accuracy, sensitivity; spesificity, positive and negative predictive values. It was concluded that, an accurate and sensitive diagnosis of pregnancy by real-time transabdominal ultrasonography can successfully be made 100% accuracy between 61-81(st) days and 94.10% accuracy between 46 and 60(th) days pregnancy in sheep.
A Serological Investigation of Pestiviruses in Sheep in Eastern Border of Turkey
(Springer, 2011) Tutuncu, Mehmet; Duz, Erkan; Karaca, Mehmet; Akkan, Hasan Altan; Keles, Ihsan; Bakir, Bahtiyar; Tasal, Ibrahim
All pestiviruses are important veterinary pathogens causing economic losses in cattle, sheep, and pigs. In this study, blood samples randomly collected from 465 sheep were analysed for the presence of antibodies to pestiviruses (bovine viral diarrhea virus, border disease virus) using an enzyme-linked immunosorbent assay in the province of Van and their towns. The seroprevalance were estimated as 75.9% and 60.0-82.5% in the sampled animals and sampled towns, respectively. The results revealed that pestiviruses are important abort pathogens in the province of Van and their towns.
Short-Acting Deslorelin Implant (Ovuplant®) Could Not Sufficiently Induce Fertile Cycles of Coloured Mohair Goats in Suckling (Anoestrous) Season
(Kafkas Univ, veteriner Fakultesi dergisi, 2012) Uslu, Baris Atalay; Sendag, Sait; Tasal, Ibrahim; Gulyuz, Fetih; Ucar, Omer; Wehrend, Axel
It is known that the pituitary FSH and LH stores are dramatically depleted during the anoestrous in seasonal breeders. Hence, the aim of this study was to evaluate the effect of deslorelin implant (a GnRH analogue) to induce oestrus in suckling goats. Suckling goats (n=21) of coloured Mohair breed were randomly assigned into two trial groups: Goats in Group I (n=11, Ovuplant (R)) received subcutaneously the implant (2.1 mg deslorelin), while animals in Group II (n=10, control) received no treatment. Along with the implant administration, serum progesterone (P-4) concentrations were determined (every other day up to 12 d) by RIA method. Within 4 days of implant administration, there were significantly higher (P<0.001) rates (100%) of pro-oestrus signs (attractiveness without mating) in Group I, than those in controls (zero %). The signs sustained for 9 days without oestrus (mating) in the vast majority (10/11) of implant-treated animals. But, an exceptional goat was in receptive oestrus commenced from the third day following the onset of pro-oestrus. Even so, there was no pregnancy post-mating. The P-4 concentrations between the groups remained below 1 ng/ml, regardless of implant treatment. Findings suggest that; i) for the P-4 concentrations, there was no marked difference between implant-treated and control groups, as remaining below 1 ng/ml all, and ii) short-acting deslorelin implant could not induce fertile oestrus cycles in coloured Mohair goats during the suckling (anoestrous) season.
Studies on the Determination of Seroprevalance of Q Fever in Sheep in the Region of Van
(Medwell online, 2009) Karaca, Mehmet; Akkan, Hasan Altan; Cetin, Yunus; Keles, Ihsan; Tutuncu, Mehmet; Ozkan, Cumali; Tasal, Ibrahim
In the present study, seroprevalance of Q fever in sheep in the region of Van was aimed to investigate. A total 465 serum samples obtained from the city of Van and its different provinces were investigated with regard to Q fever specific antibodies using ELISA technique. Out of 465 serum sample 98 of them (21.07%) were found to be positive and I I of them (2.37%) were doubtful with regard to Q fever specific antibodies.