Browsing by Author "Taskin, Abdullah"

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Peripheral Lymphocyte Dna Damage and Oxidative Status in Football Players After a Three-Day Football Tournament
(Japan Soc internal Medicine, 2013) Atli, Mustafa; Aslan, Mehmet; Kucukoglu, Mehmet Emin; Temur, Haci Bayram; Taskin, Abdullah; Celik, Hakim
Objective Regular physical activity is well known to play a cardioprotective role. The objective of this study was to investigate peripheral lymphocyte DNA damage and oxidative status in adult football players a three-day football tournament. Methods Twenty-five adult male football players and 25 sedentary male subjects were enrolled in the present study. Plasma total antioxidant status (TAS), total oxidant status (TOS) and the oxidative stress index (OSI) were determined. Peripheral lymphocyte DNA damage was determined using an alkaline comet assay. Results Plasma TOS, OSI and peripheral lymphocyte DNA damage were significantly lower in the adult football players than in the sedentary subjects (all: p<0.001), while TAS was significantly higher in the football players (p<0.001). The plasma TAS levels were inversely correlated with TOS, OSI and peripheral lymphocyte DNA damage (r =-0.683, p<0.001; r =-0.909, p<0.001; r =-0.608, p<0.001; respectively) in the adult football players. Conclusion These results indicate that physical activity is associated with increased antioxidant capacity and decreased oxidative stress. Such conditions are important for a healthy life. Further studies are needed to clarify the mechanisms underlying this association.
Peripheral Mononuclear Leukocyte Dna Damage, Plasma Prolidase Activity, and Oxidative Status in Patients With Benign Prostatic Hyperplasia
(Taylor & Francis Ltd, 2015) Gecit, Ilhan; Meral, Ismail; Aslan, Mehmet; Kocyigit, Abdurrahim; Celik, Hakim; Taskin, Abdullah; Ceylan, Kadir
Objectives: Prolidase plays a major role in collagen turnover, matrix remodeling, and cell growth. Benign prostatic hyperplasia (BPH) may be associated with an increased extracellular matrix deposition. Therefore, the present study was designed to investigate the plasma prolidase activity, oxidative status, and peripheral mononuclear leukocyte DNA damage in patients with BPH. Patients and methods: Twenty-six male patients with BPH and 24 healthy male subjects were included in this study. Blood samples were collected from antecubital vein after an overnight fasting period, and the plasma was separated. Plasma prolidase activity, total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined. The peripheral lymphocyte oxidative DNA damage was determined using an alkaline single cell gel electrophoresis assay (comet assay). Results: The plasma prolidase activity, TOS levels, OSI values, and peripheral mononuclear leukocyte DNA damage were significantly higher (P < 0.001), while the TAC levels were significantly lower (P < 0.001) in patients with BPH than controls. In BPH patients, the prolidase activity was significantly associated with TAC levels (r = -0.366, P < 0.05), TOS levels (r = 0.573, P < 0.001), and OSI (r = 0.618, P < 0.001) and peripheral mononuclear leukocyte DNA damage (r = 0.461, P < 0.001). Conclusions: Our results showed that BPH might be associated with an increased oxidative stress, and also an increased plasma prolidase activity. Increased prolidase activity might play an important role in the etiopathogenesis and/or progression of BPH.
Serum Malondialdehyde Levels, Myeloperoxidase and Catalase Activities in Patients With Nephrotic Syndrome
(Taylor & Francis Ltd, 2013) Begenik, Huseyin; Soyoral, Yasemin Usul; Erkoc, Reha; Emre, Habib; Taskin, Abdullah; Tasdemir, Mehmet; Aslan, Mehmet
Objectives: Some studies have indicated the pathophysiological importance of reactive oxygen species (ROS) in patients with nephrotic syndrome. Myeloperoxidase (MPO) is a leukocyte-derived enzyme-generating ROS that has been proposed to exert a wide array of pro-atherogenic effects throughout all stages of the atherosclerotic process. The aim of this study was to investigate the serum malondialdehyde (MDA) levels, MPO and catalase activities in patients with adult nephrotic syndrome. Patients and Methods: Twenty-four patients with nephrotic syndrome and 24 healthy controls were enrolled. Serum MPO activity, catalase activity, and MDA levels were assessed. Results: Serum MPO activity and MDA levels were significantly higher in patients with nephrotic syndrome than controls (both, P < 0.001), while catalase activity was significantly lower (P < 0.001). Serum catalase activity was found to be significantly correlated with MPO activity (r = -0.417, P = 0.003) and MDA levels (r = -0.532, P = 0.007). The serum MDA levels were also found to be significantly correlated with MPO activity (r = 0.419, P = 0.003). Conclusions: We concluded that serum MPO activity and oxidative stress were increased and that serum catalase activity was decreased in patients with adult nephrotic syndrome. In addition, these results indicate that increased MPO activity is associated with an oxidant-antioxidant imbalance that may contribute to atherosclerosis in patients with adult nephrotic syndrome.
Serum Myeloperoxidase Activity and Oxidative Stress in Patients With Acute Brucellosis
(Pergamon-elsevier Science Ltd, 2012) Karahocagil, Mustafa Kasim; Aslan, Mehmet; Ceylan, Mehmet Resat; Cikman, Aytekin; Sunnetcioglu, Mahmut; Kucukoglu, Mehmet Emin; Taskin, Abdullah
Objectives: The role of infection in the pathogenesis of atherosclerosis has been increasingly discussed. Previous studies have suggested that increased myeloperoxidase activity plays an important role in the pathogenesis of atherosclerosis. The aim of this study was to investigate the serum myeloperoxidase activity and catalase activity along with lipid hydroperoxide (LOOH) levels in patients with acute brucellosis. Design and methods: Thirty-two patients with brucellosis and 33 healthy controls were enrolled. Serum myeloperoxidase activity, catalase activity and LOOH levels were determined. Results: Serum myeloperoxidase activity and LOOH levels were significantly higher in patients with brucellosis than controls (p<0.05, p<0.001), while catalase activity were significantly lower (p<0.001). LOOH levels were found to be significantly positively correlated with MPO activity (r = 0.297, p = 0.016) in patients. Conclusions: These results indicate that increased myeloperoxidase activity and decreased catalase activity is associated with increased oxidative stress, which may have a role in atherosclerotic processes in brucellosis patients. (C) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
Serum Paraoxonase and Arylesterase Activities in Patients With Chronic Otitis Media
(Taylor & Francis Ltd, 2015) Turan, Mahfuz; Ucler, Rifki; Aslan, Mehmet; Kalkan, Ferhat; Taskin, Abdullah; Garca, Mehmet Fatih; Cankaya, Hakan
Objectives: Paraoxonase-1 (PON1) prevents oxidative stress by inhibiting the oxidation of cell membrane lipids by the reactive oxygen species that form during acute and chronic inflammation. The aim of this study was to investigate serum PON1 activity and oxidative stress in patients with chronic otitis media (COM). Methods: Fifty consecutive patients with COM and 55 controls were enrolled in the present study. The patients were divided into two groups according to the presence of cholesteatoma. The serum PON1 arylesterase activities and lipid hydroperoxide (LOOH) levels were determined. Results: Serum paraoxonase and arylesterase activities were significantly lower in the COM patients than in the controls (P < 0.001 for all comparisons), whereas the LOOH levels were significantly higher (P < 0.001). Discussion: These results indicated that a lower level of PON1 activity was associated with an oxidant-antioxidant imbalance. In addition, decreased PON1 activity may play an important role in the pathophysiology of COM.