Browsing by Author "Taskin, Bilgin"

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Antibacterial Activity of Different Kefir Types Against Various Plant Pathogenic Bacteria
(Galenos Publ House, 2020) Taskin, Bilgin; Akkopru, Ahmet
Kefir is a probiotic, dairy product produced by the fermentative activity of a diverse range of lactic acid bacteria, acetic acid bacteria, and yeast. In this study, we revealed the antimicrobial spectra of five types of kefir supernatants (EG, AN, KF, KY and SD) from different regions of Turkey fermented for 24 and 48 h against seven plant pathogenic bacteria and one bacterial biocontrol agent in vitro and in vivo for the first time. In vitro, antibacterial activity was investigated by the disk diffusion agar method. Their antibacterial potencies varied according to the type of kefir and the fermentation time. Also, we showed that the antimicrobial activity of kefir could be attributable to antimicrobial substances in supernatants rather than the low pH. In vivo, studies using the most potent kefir type on cucumber and common bean with their pathogenic bacteria in the climate chamber showed no remarkable decrease in diseases but revealed an increase in some plant growth parameters. The application resulted in an increase of 22% in shoot fresh weight, 20% in shoot dry weight, 79% in root fresh weight and 113% in root dry weight in common bean, on the other hand, 25% in shoot fresh weight, 34% in root fresh weight and 30% in shoot dry weight in cucumber.
Application of Ipbs-Retrotransposons Markers for the Assessment of Genetic Diversity and Population Structure Among Sugar Beet (Beta Vulgaris) Germplasm From Different Regions of the World
(Springer, 2025) Sadik, Gokhan; Yildiz, Mehtap; Taskin, Bilgin; Kocak, Metin; Cavagnaro, Pablo Federico; Baloch, Faheem Shehzad
Sugar beet is an important agricultural crop product that has been produced and consumed worldwide since the eighteenth century and can adapt to various climatic and soil conditions. The two fundamental building blocks of any crop improvement program are germplasm resources, which contain genetic diversity and phenotypic expression of desired traits. In this study, a total of 58 sugar beet genotypes including 12 from Turkey, 4 from India, 12 from the United States of America, 16 from Iran, 12 from England and Beta vulgaris L. subsp. maritima L. Arcang. as wild species were characterized using 15 inter-primer binding site (iPBS) markers that produced intense and polymorphic bands in the germplasm library. Using these 15 iPBS markers, 102 polymorphic bands were produced and the average number of polymorphic bands was determined as 6.8. Polymorphism information content (PIC) values ranged between 0.58 and 0.83, and the average PIC value was found to be 0.70. It was determined that the most genetically different genotypes were PI 590697-US11 and PI 171508-TR8, with a distance of 0.73. Clustering algorithms Unweighted Pair Group Method Algorithm (UPGMA) and Principal Coordinate Algorithm (PCoA) confirmed that genotypes are an important factor in clustering, and STRUCTURE analysis divided sugar beet gene resources into six populations. Also, the analysis of molecular variance (AMOVA) showed that there was 8% variance among populations and 92% variance within populations. This is the first study to investigate the genetic diversity and population structure of sugar beet germplasm using the iPBS-retrotransposon marker system. The results of this research emphasized that iPBS markers are very successful and effective in examining the genetic diversity of sugar beet germplasm. The results obtained in this study provide a theoretical basis for future selection and breeding of superior sugar beet germplasm sources.
Exploring the Genetic Variations and Population Structure of Turkish Pepper (Capsicum Annuum L.) Genotypes Based on Peroxidase Gene Markers
(Springer Heidelberg, 2018) Akyavuz, Rifat; Taskin, Bilgin; Kocak, Metin; Yildiz, Mehtap
Capsicum is thought as one of the most diverse and significant genera due to its varied uses in different parts of the world. In this study, we worked with a total of 71 pepper genotypes from different locations of Turkey to investigate the level of their diversity using the peroxidase gene polymorphism (POGP) markers to reveal their population structure. For this purpose, 14 peroxidase primer pairs were used. They produced 139 bands (mean = 9.9 bands/primer), of which similar to 85.6% were polymorphic in the all germplasm collection. Polymorphism information content (PIC) ranged between 0.48 and 0.97 with an average of 0.75. Range and mean values for gene diversity (h) were 0.09-0.22 and 0.17, respectively. Shannon's information index (I) per POGP marker ranged from 0.18 to 0.35 with a mean of 0.29. Using three clustering methods (unweighted pair-group method with arithmetic means, principal coordinate analysis, and STRUCTURE) revealed a clear separation of all the C. annuum accessions from C. frutescens and C. chinense accessions in our study. Clusters did not establish an association between the accessions and their geographical origin. This is the first study exploring the population structure through the genetic diversity of Turkish peppers from different regions of the country based on the peroxidase gene markers.
Gökkuşağı Alabalıklarından (Oncorhynchus Mykiss) İzole Edilen Patojen Bakteri İzolatlarının Hidrolitik Enzim Aktiviteleri ve Siderofor Üretim Yetenekleri
(2022) Taskin, Bilgin; Önalan, Şükrü
Kısa sürede büyük miktarlarda kültürlenebilmeleri, genetik manipülasyonlara açık olmaları ve ürettikleri enzimlerin hayvansal ve bitkisel enzimlerden daha aktif ve istikrarlı oluşu bakterilerin çok önemli enzim ve ikincil metabolit kaynakları olarak değerlendirilmelerine sebep olmaktadır. Ayrıca ekstraselüler hidrolitik enzimler ve sideroforlar patojen bakteriler için çok önemli virülans faktörleri olarak bilinmektedir. Bu çalışmada, Van ilinde faaliyet gösteren çiftliklerde, gökkuşağı alabalıklarından (Oncorhynchus mykiss) izole edilerek kültüre alınmış dokuz adet bakteri izolatının çeşitli ekstraselüler hidrolitik enzimleri ve siderofor üretme yetenekleri ilk defa araştırılmıştır. Sonuçlar, test edilen izolatlardan dokuzunun proteaz, sekizinin lipaz, beşinin selülaz, ikisinin pektinaz aktivitesine sahip olduğu, ayrıca yedisinin siderofor üretme yeteneğine sahip olduğunu göstermiştir. Amilaz ve ksilinaz aktiviteleri hiçbir izolatta gözlemlenmemiştir. Ayrıca bu izolatlar morfolojik olarak olası üç ana gruba ayrılmış ve her bir izolat, türe özel geliştirilmiş primerler ile moleküler tanımlamaya tabi tutulmuştur. Türe özgü primerler kullanılarak yapılan moleküler tanımlama, bu izolatların balık patojeni Listonella anguillarum, Yersinia ruckeri ve Lactococcus garvieae olduklarını ortaya koymuştur.
High Level Multiple Antibiotic Resistance Among Fish Surface Associated Bacterial Populations in Non-Aquaculture Freshwater Environment
(Pergamon-elsevier Science Ltd, 2012) Ozaktas, Tugba; Taskin, Bilgin; Gozen, Ayse G.
Freshwater fish, Alburnus alburnus (bleak), were captured from Lake Mogan, situated in Ankara, during spring. The surface mucus of the fish was collected and associated bacteria were cultured and isolated. By sequencing PCR-amplified 16S RNA encoding genes, the isolates were identified as members of 12 different genera: Acinetobacter, Aeromonas, Bacillus, Brevundimonas, Gordonia, Kocuria, Microbacterium, Mycobacterium, Pseudomonas, Rhodococcus, and Staphylococcus, in addition to one strain that was unidentified. The mucus-dwelling bacterial isolates were tested for resistance against ampicillin, kanamycin, streptomycin and chloramphenicol. About 95% of the isolates were found to be resistant to ampicillin, 93% to chloramphenicol, and 88% to kanamycin and streptomycin. A Microbacterium oxydans and the unidentified environmental isolate were resistant to all four antibiotics tested at very high levels (>1600 mu g/ml ampicillin and streptomycin; >1120 mu g/ml kanamycin; >960 mu g/ml chloramphenicol). Only a Kocuria sp. was sensitive to all four antibiotics at the lowest concentrations tested (3.10 mu g/ml ampicillin and streptomycin; 2.15 mu g/ml kanamycin; 1.85 mu g/ml chloramphenicol). The rest of the isolates showed different resistance levels. Plasmid isolations were carried out to determine if the multiple antibiotic resistance could be attributed to the presence of plasmids. However, no plasmid was detected in any of the isolates. The resistance appeared to be mediated by chromosome-associated functions. This study indicated that multiple antibiotic resistance at moderate to high levels is common among the current phenotypes of the fish mucus-dwelling bacterial populations in this temperate, shallow lake which has not been subjected to any aquaculturing so far but under anthropogenic effect being in a recreational area. (C) 2012 Elsevier Ltd. All rights reserved.
Hydrolytic Enzymes Producing Bacterial Endophytes of Some Poaceae Plants
(Polskie Towarzystwo Mikrobiologow-polish Society of Microbiologists, 2021) Dogan, Gokhan; Taskin, Bilgin
Endophytic bacteria represent microorganisms that live during the whole life cycle within the tissues of healthy plants without causing any obvious signs of disease. In this study, the ability of 128 endophyte bacterial isolates from some cultivated and wild grain plants (Poaceae family) in Van, Turkey, were investigated in terms of producing several extracellular hydrolytic enzymes. It was demonstrated that lipases, proteases, amylases, cellulases, pectinases, and xylanases were produced by the bacteria with relative frequencies of 74.2%, 65.6%, 55.4%, 32%, 21.8%, and 7.8%, respectively. In addition, molecular identification of a certain number of isolates selected according to their enzyme-producing capabilities was performed by 16S rRNA gene sequencing using a next-generation sequencing platform. As a result of the analysis, the isolates yielded certain strains belonging to Pseudomonas, Micrococcus, Paenibacillus, Streptococcus, Curtobacterium, Chryseobacterium, and Bacillus genera. Also, the strain G117Y1T was evaluated as a member of potential novel species based on 16S rRNA sequencing results.
Isolation and Characterization of Endophytic Bacteria From the Poaceae Family: Assessing Their Impact on Drought Stress and Plant Growth in Wheat
(Springer, 2025) Akkopru, Ahmet; Taskin, Bilgin; Arpali, Digdem; Ucar, Ceylan Pinar; Yardim, Dilek Ozcan; Ozturkci, Yusuf
Limited water resources and the increasing impact of global warming highlight the importance of endophytic bacteria (EB) given their unique tolerance to drought stress. In this study, we isolated EB from cultivated and wild Poaceae family members grown in arid or challenging environments in Lake Van Basin. We aimed to reveal plant growth promoting (PGP) characters in EB and to determine their potential to promote plant growth and reduce the effect of different drought levels in two different wheat cultivars (Tir (tolerant) and Bezostaja-1). 410 EB were isolated from 135 plants belonging to 19 taxa at different periods. In vitro tests revealed that 73.1% of the isolates can synthesize indole acetic acid (IAA), 34.4% can fix nitrogen, 37% can synthesize 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (ACCd), 37.6% can solubilize phosphorus, and 93.9% can synthesize siderophore. IAA production and phosphorous solubility were the most common characters, coexisting in 59% of EBs. ACCd production, phosphorus solubilization, and nitrogen fixation were the most common features with a triple association of 0.49%. Although isolates selected according to EB characters showed differing effects on coleoptile, root and shoot length, germination percentage, shoot and root weight, and relative water content (RWC) in the early development period (EDP) of Bezostaja-1 cultivar under drought stress, some isolates had negative effects. However, Pseudomonas asturiensis G58S1, Pantoea agglomerans G129K1-1, Chryseobacterium rhizosphaerae G129S2, Acinetobacter calcoaceticus G118K1T, and Stenotrophomonas sp. G109K2-K isolates selected according to PGP trials and EDP results showed improved plant biomass, chlorophyll content, membrane permeability, germination power, and RCW, and protective effects against drought.
Kefiran Ekstraktının Bazı Bitki Patojeni Bakterilerine Karşı Antimikrobiyal Etkisinin Değerlendirilmesi
(2020) Taskin, Bilgin
Kefir; laktik asit bakterileri, asetik asit bakterileri ve mayalar gibi çok çeşitli mikroorganizmalarıbarındıran granüller tarafından üretilen, pek çok ülkede geleneksel olarak tüketilen fermente bir sütiçeceğidir. Granülleri çevreleyen ve ağırlıklı olarak kefiran molekülünden oluşan polisakkarityapının sağlığı teşvik edici birçok etkisinin yanında çeşitli patojenlere karşı antimikrobiyal etkiyesahip olduğu bildirilmektedir. Bu çalışmada, biri ticari olarak satılan, diğeri yerel bir satıcıdan teminedilen granüller kullanılarak 24 saatlik fermantasyonla kefir üretimi yapılmıştır. Olgunlaşan kefirtanelerinden kefiran ekstraktı eldesi ve saflaştırılması etanol presipitasyon yötemi ile 80°C, 90°C ve100°C olmak üzere üç farklı sıcaklıkta gerçekleştirilmiştir. Ayrıca elde edilen ekstraktsolüsyonlarının protein içerikleri Bradford yöntemiyle ile belirlenmiştir. Denemelerde kullanılanekstrakt solüsyonlarının protein içeriği 0,001 g/mL olarak ölçülmüştür. Oluşturulan stoksolüsyonlarından, 0,05, 0,1, 1 ve 2 mg ekstrakt içeren uygulamaların Pseudomonas, Xanthomonas,Erwinia ve Clavibacter cinslerine ait bazı bitki patojeni bakterilere karşı antibakteriyel etkisi in vitrokoşullarda ilk defa incelenmiştir. Bu amaçla disk difüzyon metodu ve yayma yöntemi olmak üzereiki metot kullanılmıştır. Denemelerde pozitif kontrol olarak kullanılan AN kefir süpernatantıizolatlar üzerinde ortalama 13-17 mm inhibisyon zonu gösterirken, SD süpernatantı 10-14 mm zonüretmiş ancak kefiran ekstralarının antibakteriyel etkisi gözlemlenmemiştir.
Start-Up Strategies of Electromethanogenic Reactors for Methane Production From Cattle Manure
(Pergamon-elsevier Science Ltd, 2023) Ghaderikia, Amin; Taskin, Bilgin; Yilmazel, Yasemin Dilsad
This study qualitatively assessed the impacts of different start-up strategies on the performance of methane (CH4) production from cattle manure (CM) in electromethanogenic reactors. Single chamber MECs were operated with an applied voltage of 0.7 V and the impact of electrode acclimatization with a simple substrate, acetate (ACE) vs a complex waste, CM, was compared. Upon biofilm formation on the sole carbon source (ACE or CM), several MECs (ACE_CM and CM_ACE) were subjected to cross-feeding (switching substrate to CM or ACE) during the test period to evaluate the impact of the primary substrate. Even though there was twice as much peak current density via feeding ACE during biofilm formation, this did not translate into higher CH4 production during the test period, when reactors were fed with CM. Higher or similar CH4 production was recorded in CM_CM reactors compared to ACE_CM at various soluble chemical oxygen demand (sCOD) concentrations. Additionally, feeding ACE as primary substrate did not significantly impact either COD removals or coulombic efficiencies. On the other hand, the use of anaerobic digester (AD) seed as an inoculum in CM-fed MECs (CM_CM), relative to no inoculum added MECs (Blank), increased the initial CH4 production rate by 45% and reduced the start-up time by 20%. In CM-fed MECs, Geobacter dominated bacterial communities of bioanodes and hydrogenotrophic methanogen Methanoculleus dominated archaeal communities of biocathodes. Community cluster analysis revealed the significance of primary substrate in shaping electrode biofilm; thus, it should be carefully selected for successful start-up of electromethanogenic reactors treating wastes.