Browsing by Author "Taskiran, Aysegul"

Now showing 1 - 3 of 3

Embryonic Microenvironment Suppresses Yy1 and Yy1-Related Genes in Prostate Cancer Stem Cells
(Elsevier Gmbh, 2024) Taskiran, Aysegul; Oktem, Gulperi; Demir, Aleyna; Oltulu, Fatih; Ozcinar, Emine; Duzagac, Fahriye; Acikgoz, Eda
Yin yang 1 (YY1), a transcription factor, plays crucial roles in cell fate specification, differentiation, and pluripotency during embryonic development. It is also involved in tumorigenesis, drug resistance, metastasis, and relapse caused by cancer stem cells (CSCs), particularly in prostate cancer (PCa). Targeting YY1 could potentially eliminate prostate CSCs (PCSCs) and provide novel therapeutic approaches. PCa tissues often exhibit elevated YY1 expression levels, especially in high-grade cases. Notably, high-grade PCa tissues from 58 PCa patients and CD133high/CD44high high /CD44 high PCSCs isolated from DU145 PCa cell line by FACS both showed significantly increased YY1 expression as observed through immunofluorescence staining, respectively. To investigate the embryonic microenvironment impact on YY1 expression in CSC populations, firstly PCSCs were microinjected into the inner cell mass of blastocysts and then PCSCs were co-cultured with blastocysts. Next Generation Sequencing was used to analyze alterations in YY1 and related gene expressions. Interestingly, exposure to the embryonic microenvironment significantly reduced the expressions of YY1, YY2, and other relevant genes in PCSCs. These findings emphasize the tumor-suppressing effects of the embryonic environment by downregulating YY1 and YY1-related genes in PCSCs, thus providing promising strategies for PCa therapy. Through elucidating the mechanisms involved in embryonic reprogramming and its effects on YY1 expression, this research offers opportunities for further investigation into focused therapies directed against PCSCs, therefore enhancing the outcomes of PCa therapy. As a result, PCa tumors may benefit from YY1 and associated genes as a novel therapeutic target.
Optimized Method for Using Embryonic Microenvironment To Reprogram Cancer Stem Cells
(Dokuz Eylul Univ inst Health Sciences, 2023) Soner, Burak Cem; Oltulu, Fatih; Ozcinar, Emine; Taskiran, Aysegul; Demir, Aleyna; Acikgoz, Eda; Oktem, Gulperi
Purpose: The embryonic microenvironment contains many properties that have not yet been fully explored. Our aim in this study is to report an optimized and efficient method that enables investigating the effects of the secretome of pluripotent embryonic stem cells on cancer stem cells.Material and Methods: The study is performed with a chimeric model consisted of mouse blastocysts, non cancer stem cells and human prostate cancer stem cells. Ovulation induced mice were used for blastocyst collection. DU145 prostate cancer cell line was separated into non cancer and cancer stem cells using cancer stem cell biomarker expressions by fluorescent activated cell sorting. Human prostate cancer stem cells and non cancer stem cells were microinjected into 4-day blastocyst culture in vitro by intracytoplasmic sperm injection.Results: Chimeric models provide us great convenience in basic oncological studies. In this study, using a chimeric model, we were able to study the secretome of mouse embryonic stem cells and their effect on cancer stem cells. The method is efficient and yield promising result; and could be used to study the effects on other cells as well.Conclusion: The embryonic stem cell microenvironment is suggested to have a great regenerative capacity, nowadays, the center of attraction for cancer research studies. Ethical issues restrict the human embryo studies, however, mimicking the in vivo human microenvironment with 3D cell cultures or bioprinting are now possible. Finally, optimization of new methods including 3D cell cultures with human cell lines will be a great opportunity for better understanding the reprogramming notion.
Tgf-β1 Uygulaması Sonrasında Prostat Kanseri Hücrelerinde Versicanın Farklı Mrna ve Protein Ekspresyonu
(2022) Acikgoz, Eda; Soner, Burak; Taskiran, Aysegul; Caggia, Silvia; Khan, Shafıq; Oktem, Gulperi
Amaç: Tümörün hücresel heterojenliği, kusurlu genetik ve epigenetik ağlar nedeniyle bir dizi eşsiz protein eksprese eden hücre popülasyonlarından kaynaklanır. Versicanın (VCAN), çeşitli kanser hücre tiplerinde transforme edici büyüme faktör-beta (TGF-β1) tarafından up regüle edildiği gösterilmiştir. Daha önceki çalışmalarımızda, kanser kök hücre (CSC’ler) tek katmanlı kültürlerinde TGF-β1 ekspresyonunun yükseldiğini ve CSC’lerden oluşan sferoidlerde VCAN ekspresyonlarının da önemli ölçüde arttığını ortaya koydu. Yöntem: Bu sonuçlar dahilinde monolayer yüksek TGF-β1 ekspresyonunun VCAN ekspresyonunu uyararak üç boyutlu yapılanmasını tetikleyebileceği varsayılmıştır. Bu çalışma, primer ve sekonder tümör derive hücre dizilerinde VCAN'ın ekspresyon profilini ve bu hücrelerde TGF-β1 sinyalleşmesinin etkisini araştırdı.Bulgular: PC3 insan prostat hücre dizisindeki VCAN gen ekspresyon düzeyinin western blot analizinde VCAN protein ekspresyonu ile korele olduğunu gösterdi. TGF-β1’in VCAN protein ekspresyonu üzerinde hiçbir indükleyici veya baskılayıcı etkisi yoktu. Sonuç: TGF-β1’in bu çalışmada kullanılan dozlarda prostat kanseri hücrelerinde VCAN ekspresyonu üzerinde uyarıcı ya da engelleyici etkileri yoktur. Metastatik yüksek VCAN protein seviyeleri gözlenirken, artmış protein stabilitesi ve/veya prostat karsinomunda sekonder tümör hücrelerinde önemli bir rol oynayabilecek farklı VCAN izoformlarının ekspresyonu gibi transkripsiyon sonrası değişikliklerden kaynaklanabilecek hiçbir mRNA tespit edilmedi.