Browsing by Author "Taspinar, M."

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Comparative Evaluation of the Cytotoxic Effects of Different Oral Antiseptics: a Primary Culture Study
(Wolters Kluwer Medknow Publications, 2021) Alpaslan Yayli, N. Z.; Tunc, S. Keskin; Degirmenci, B. Unalan; Dikilitas, A.; Taspinar, M.
Background: Dental plaque is a major oral health problem with severe consequences. Oral antiseptics provide important means for controlling dental plaque formation and are widely used by the public. However, some of these antiseptics have been shown to have side effects on oral tissues. Aim: In this study, we aimed to investigate the time and dose-dependent cytotoxic effects of various antiseptics on primary human gingival fibroblasts (HGF). Methods: HGF cells were obtained using primary culture techniques. The effects of various doses of 5 antiseptics containing Chlorhexidine-Gluconate (CHX), CHX with Benzydamine-Hydrochloride (Benzydamine-HCl), Povidone-Iodine (PVP-I), Benzydamine-HCl and Essential-Oil on HGFs were analyzed by using 2,3-bis (2-metoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide cell viability assay after 30, 60, and 180 s of exposure. Results: Cell viability analyses showed that cell death increased in an application time and dose-dependent manner. There was a statistically significant difference in the effects of each antiseptic on live-cell densities compared to the control group and each other (P < 0.001). Antiseptic containing 0.2% CHX showed the highest cytotoxicity on cells. The remaining viable cell density after administration of 0.2% CHX at a dose of 12.5% for 30 s is 35.19%. The high cytotoxic effect of 0.2% CHX was followed by 0.12% CHX with 0.15% Benzydamine-HCl, PVP-I and 0.15% Benzydamine-HCl groups. The lowest cytotoxic effect was observed for the Essential-Oil containing antiseptic solution. Conclusions: The results of this study show that these five antiseptic agents have variable effects on in vitro HGF proliferation. The doses and administration times of antiseptics should be controlled carefully during dental applications.
The Effect of Nonsurgical Periodontal Treatment on Serum and Gingival Crevicular Fluid Markers in Patients With Atherosclerosis
(Medknow Publications & Media Pvt Ltd, 2017) Ertugrul, A. S.; Bozoglan, A.; Taspinar, M.
Background and Aims: The aim of this study is to compare patients with atherosclerosis and chronic periodontitis and patients who are systemically healthy and chronic periodontitis using alteration of adrenomedullin (ADM), chemokine (C-C motif) ligand 28 (CCL-28), white blood cell levels, platelet levels, high-density lipoprotein, low-density lipoprotein, high-sensitivity C-reactive protein, creatinine, and fibrinogen. Materials and Methods: Totally, 40 patients were involved in study; a test group of 20 patients with atherosclerosis-chronic periodontitis and a control group of 20 patients who were nonatherosclerosis-chronic periodontitis. Nonsurgical periodontal treatment was offered to all patients, in whom systemic markers of atherosclerosis were measured in serum; ADM and CCL-28 biomarkers were measured in gingival crevicular fluid. Results: Systemic markers of atherosclerosis, ADM, and CCL-28 levels have changed significantly in the test group compared to the control group after nonsurgical periodontal treatment. Conclusions: Treatment of local inflammation and reduction of systemic inflammatory markers are believed to lower the diagnostic criteria for atherosclerosis as well. It is possible to conclude that nonsurgical periodontal treatment of chronic periodontitis, which is a risk factor for atherosclerosis, has a positive effect on the atherosclerosis prognosis.
The Effects of Vitamin D Onto the Expression of Caspase Enzymes in Osteoblastic Cell Line Treated With Sodium Fluoride (Naf)
(Wiley, 2017) Yuksek, V.; Dede, S.; Taspinar, M.
Impact of Follicle-Stimulating Hormone Receptor Variants in Female Infertility
(Springer New York LLC, 2015) Ilgaz, N.S.; Aydos, O.S.E.; Karadag, A.; Taspinar, M.; Eryilmaz, O.G.; Sunguroglu, A.
Purpose: Follicle-stimulating hormone (FSH) and its receptor play a major role in the development of follicles and regulation of steroidogenesis in the ovary and spermatogenesis in the testis. We aim to analyze the role of FSHR gene variants (single nucleotide polymorphisms (SNPs) in exon 10 (codon 307 and 680) and in the core promoter region (at position −29) and Ala189Val inactivating mutation) in Turkish infertile women. There were studies analyzing the effects of the SNPs in exon 10 (codon 307 and 680) and in the core promoter region (at position −29) of the FSHR gene on spermatogenesis, but to our knowledge, there were no studies analyzing the effects of these three SNP combinations on female fertility. Methods: In this study, the allelic, genotype, and haplotype frequency distributions of these three SNPs in the FSHR gene were analyzed in 102 infertile women and 99 unrelated healthy control individuals. The distribution of the polymorphisms was conformed by Hardy–Weinberg equilibrium test. Results: There were no statistical differences (P > 0.05) in the allele, genotype, and haplotype frequencies of the polymorphisms and FSH, luteinizing hormone (LH), estradiol (E2), and prolactin (PRL) levels between the infertile patients and the controls. However, a significant relation was found between 307 SNP GA genotype and FSH level ≥12. We did not find any homozygous or heterozygote mutations in infertile patients and healthy fertile controls. Conclusion: The present study was the first study analyzing gma mutation and the polymorphism of the FSHR core promoter at position −29 alone and in combination with the two common SNPs in exon 10 in Turkish infertile women population. These findings indicate the significance of Ala307Thr GA genotype may be a predictive marker for poor ovarian reserve and infertility. © 2015, Springer Science+Business Media New York.
In Vitro Evaluation of the Apoptotic, Autophagic, and Necrotic Molecular Pathways of Fluoride
(Humana Press inc, 2021) Urut, F.; Dede, S.; Yuksek, V.; Cetin, S.; Usta, A.; Taspinar, M.
Prolonged exposure to high doses of fluoride causes chronic poisoning called fluorosis, which affects many tissues and causes serious health problems. This study was planned to investigate the apoptotic, autophagic, and necrotic molecular pathways of fluoride. Sodium fluoride (NaF) was administered to normal rat kidney epithelial (NRK-52E) cells. The NaF IC50 value was determined using the MTT assay. The expression of the genes in the autophagic, apoptotic, and necrotic pathways was determined by real-time PCR. It was determined that there were significant changes in NaF-induced molecular pathways depending on the time. There were no increases in apoptotic and necrotic pathway markers except for Atg3, an autophagy gene, at the 3rd and the 12th hours. However, there was an induction in all cell death signaling pathways at 24 h. The molecular mechanisms demonstrated NaF-induced cellular death in the NRK-52E cell line. It was concluded that these molecular mechanisms were activated with NaF, and different mechanisms accelerated the cellular death at the 24th hour.
In Vitro Evaluation of the Apoptotic, Autophagic, and Necrotic Molecular Pathways of Fluoride (Nov, 2020, 10.1007/S12011-020-02491-3)
(Springernature, 2021) Urut, F.; Dede, S.; Yuksek, V.; Cetin, S.; Usta, A.; Taspinar, M.
Lycopene and Resveratrol Improve Post-Thaw Bull Sperm Parameters: Sperm Motility, Mitochondrial Activity and Dna Integrity
(Wiley, 2015) Bucak, M. N.; Ataman, M. B.; Baspinar, N.; Uysal, O.; Taspinar, M.; Bilgili, A.; Akal, E.
We focussed on evaluating the protective effect of lycopene and resveratrol on post-thaw bull sperm and oxidative stress parameters. Nine ejaculates for each bull were used in the study. Each ejaculate, splitted into three equal aliquots and diluted at 37 degrees C with base extenders containing lycopene (1x10(-3)gml(-1)) and resveratrol (1mm), and no antioxidant (control), was cooled to 5 degrees C and then frozen. Frozen straws were thawed in a water bath for evaluation. The supplementation of the semen extender with lycopene and resveratrol increased the percentages of post-thawed computer-assisted sperm analysis (CASA) motility (55.8 +/- 3.8 and 61.9 +/- 4.0%) and progressive motility (38 +/- 2.4 and 37 +/- 8.8), compared with the controls (50.7 +/- 2.65 and 33.3 +/- 3.74%, respectively, P<0.05). Resveratrol provided a higher ALH (4.3 +/- 0.1), in comparison with the control (3.9 +/- 0.3, P<0.05). The supplementation of the semen extender with lycopene and resveratrol produced a higher mitochondrial activity (24.6 +/- 2.9 and 30.1 +/- 6.5% respectively), compared with that of the control (11.8 +/- 9.5%, P<0.05). It was determined that both antioxidants resulted in a lower percentage of sperm with damaged DNA than that of the control (P<0.05). Sperm motion characteristics except for ALH, acrosome integrity, sperm viability and oxidative stress parameters were not affected by the adding of lycopene and resveratrol.
The Role of Matrix Metalloproteinase as Biomarkers for Neural Tube Defect
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Akyol, M.E.; Ebiri, F.N.D.; Taspinar, F.; Yüksek, V.; Tuncer, O.; Arslan, M.; Taspinar, M.
Neural Tube Defect (NTD) is one of the most common congenital malformations. It is crucial to determine the prognostic, predictive, or therapeutic genetic factors for preventing NTD. The formation of the extracellular matrix (ECM) plays an essential role in migrating neural crest cells. Matrix metalloproteinases (MMPs) play a significant role in cell migration in ECM organization. The role of expressions and activation of MMP in NTD is unknown. This study aimed to investigate the roles of MMP-1,-2, and 9 gene expressions as biomarkers for NTD. Peripheral blood samples and NTD tissues were collected from 40 newborn babies diagnosed with NTD, which were also divided into subgroups based on pathology, and peripheral blood samples from only 20 healthy babies were taken for control. After total RNA isolation from blood and tissues, MMP-1,-2,-9 gene expressions were analyzed by Quantitative Real-Time PCR (RT-PCR). There was no difference between the control group and the NTD group in terms of MMP expressions in blood samples (p>0.05). A statistically significantly higher MMP-1 expression was found in Meningocele and Myeloschisis than in Encephalocele (p=0.014). A significant difference was found between the tissue and blood samples of the Meningomyelocele patient group regarding MMP-9 expression (p=0.019). There was no significant relationship between Ca2+, B12, and Folate levels, NTD, and MMP genes expressions (p>0.05). Even though MMP genes were not different between control and NTD groups, they were found to vary between different subgroups and can serve as biomarkers. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
The Use of Methylene Blue as Mouthwash in Periodontology
(Yuzuncu Yil Universitesi Tip Fakultesi, 2015) Burak, A.K.; Özeroğlu, E.; Taspinar, M.
The aim of this study is to investigate the cytotoxicity effects of methylene blue on the human gingival fibroblasts cell-lines in vitro. 3T3 human gingival fibroblast cell-lines were used to evaluate the cytotoxicity of mouthwashes containing methylene blue and chlorhexidine gluconate. The cultured fibroblasts were divided into two groups which subjected into chlorhexidine gluconate or methylene blue in vitro. The cells viability was determined at 30 seconds, 1 minute and 3 minutes of exposure to mouthwashes using by XTT colorimetric assay. Spectrophotometric absorbance was measured at 550 nm using ELISA analyzer. The IC50 values were calculated for each time points for methylene blue and chlorhexidine gluconate mouthwashes. The effect of the methylene blue and chlorhexidine gluconate on the human gingival fibroblast viability was expressed as a percentage of the control groups. Comparison between two groups in different time and concentration values showed that, chlorhexidine gluconate were found to be more cytotoxic on gingival fibroblast than methylene blue. Cell viability exposured to methylene blue in 1% concentration during 3 minutes was 99% and in 100% concentration the cell viability was 88%. The chlorhexidine gluconate at the same conditions was 92% and 18% respectively. Our study demonstrates that 100% chlorhexidine gluconate (0.2% chlorhexidine gluconate concentration in commercially available products) has much more cytotoxic effect than methylene blue to human gingival fibroblast at clinical use time and different concentrations in vitro. © 2015, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.