Browsing by Author "Teker, Hikmet Taner"

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Age-Related Differences in Response To Plasma Exchange in Male Rat Liver Tissues: Insights From Histopathological and Machine-Learning Assisted Spectrochemical Analyses
(Springer, 2023) Teker, Hikmet Taner; Ceylani, Taha; Keskin, Seda; Samgane, Gizem; Mansuroglu, Sina; Baba, Burcu; Gurbanov, Rafig
This study aimed to examine the biological effects of blood plasma exchange in liver tissues of aged and young rats using machine learning methods and spectrochemical and histopathological approaches. Linear Discriminant Analysis (LDA) and Support Vector Machine (SVM) were the machine learning algorithms employed. Young plasma was given to old male rats (24 months), while old plasma was given to young male rats (5 weeks) for thirty days. LDA (95.83-100%) and SVM (87.5-91.67%) detected significant qualitative changes in liver biomolecules. In old rats, young plasma infusion increased the length of fatty acids, triglyceride, lipid carbonyl, and glycogen levels. Nucleic acid concentration, phosphorylation, and carbonylation rates of proteins were also increased, whereas a decrease in protein concentration was measured. Aged plasma decreased protein carbonylation, triglyceride, and lipid carbonyl levels. Young plasma infusion improved hepatic fibrosis and cellular degeneration and reduced hepatic microvesicular steatosis in aged rats. Otherwise, old plasma infusion in young rats caused disrupted cellular organization, steatosis, and increased fibrosis. Young plasma administration increased liver glycogen accumulation and serum albumin levels. Aged plasma infusion raised serum ALT levels while diminished ALP concentrations in young rats, suggesting possible liver dysfunction. Young plasma increased serum albumin levels in old rats. The study concluded that young plasma infusion might be associated with declined liver damage and fibrosis in aged rats, while aged plasma infusion negatively impacted liver health in young rats. These results imply that young blood plasma holds potential as a rejuvenation therapy for liver health and function.
Promoting Longevity in Aged Liver Through Nlrp3 Inflammasome Inhibition Using Tauroursodeoxycholic Acid (Tudca) and Scd Probiotics
(Elsevier Ireland Ltd, 2024) Baba, Burcu; Ceylani, Taha; Gurbanov, Rafig; Acikgoz, Eda; Allahverdi, Hueseyin; Keskin, Seda; Teker, Hikmet Taner
This investigation explores the combined influence of SCD Probiotics and tauroursodeoxycholic acid (TUDCA) on liver health in elderly male Sprague-Dawley rats. Through the administration of intravenous TUDCA (300 mg/ kg) and oral SCD Probiotics (3 mL at 1 x 10<^>8 CFU) daily for one week, this study evaluates the biomolecular composition, histopathological alterations, and inflammasome activity in the liver. Analytical methods encompassed ATR-FTIR spectroscopy integrated with machine learning for the assessment of biomolecular structures, RT-qPCR for quantifying inflammasome markers (NLRP3, ASC, Caspase-1, IL18, IL1 beta), and histological examinations to assess liver pathology. The findings reveal that TUDCA prominently enhanced lipid metabolism by reducing cholesterol esters, while SCD Probiotics modulated both lipid and protein profiles, notably affecting fatty acid chain lengths and protein configurations. Histological analysis showed significant reductions in cellular degeneration, lymphatic infiltration, and hepatic fibrosis. Furthermore, the study noted a decrease in the immunoreactivity for NLRP3 and ASC, suggesting suppressed inflammasome activity. While SCD Probiotics reduced the expression of certain inflammasome-related genes, they also paradoxically increased AST and LDH levels. Conversely, an exclusive elevation in albumin levels was observed in the group treated with SCD Probiotics, implying a protective role against liver damage. These results underscore the therapeutic potential of TUDCA and SCD Probiotics for managing age -associated liver disorders, illustrating their individual and synergistic effects on liver health and pathology. This study provides insights into the complex interactions of these agents, advocating for customized therapeutic approaches to combat liver fibrosis, enhance liver functionality, and decrease inflammation in aging populations.
Reduced Liver Damage and Fibrosis With Combined Scd Probiotics and Intermittent Fasting in Aged Rat
(Wiley, 2024) Teker, Hikmet Taner; Ceylani, Taha; Keskin, Seda; Samgane, Gizem; Baba, Burcu; Acikgoz, Eda; Gurbanov, Rafig
This study aimed to examine the impact of SCD Probiotics supplementation on liver biomolecule content and histological changes during a 30-day intermittent fasting (IF) program in 24-month-old male Sprague-Dawley rats. Rats underwent 18-h daily fasting and received 1 x 10(8) CFU of SCD Probiotics daily. Liver tissue biomolecules were analysed using FTIR Spectroscopy, LDA, and SVM techniques, while histopathological evaluations used Haematoxylin and eosin and Masson trichrome-stained tissues. Blood samples were collected for biochemical analysis. Gross alterations in the quantity of biomolecules were observed with individual or combined treatments. LDA and SVM analyses demonstrated a high accuracy in differentiating control and treated groups. The combination treatments led to the most significant reduction in cholesterol ester (1740 cm(-1)) and improved protein phosphorylation (A(1239)/A(2955) and A(1080)/A(1545)) and carbonylation (A(1740)/A(1545)). Individually, IF and SCD Probiotics were more effective in enhancing membrane dynamics (Bw(2922)/Bw(2955)). In treated groups, histological evaluations showed decreased hepatocyte degeneration, lymphocyticinfiltration, steatosis and fibrosis. Serum ALP, LDH and albumin levels significantly increased in the SCD Probiotics and combined treatment groups. This study offers valuable insights into the potential mechanisms behind the beneficial effects of IF and SCD Probiotics on liver biomolecule content, contributing to the development of personalized nutrition and health strategies.
The Rejuvenating Influence of Young Plasma on Aged Intestine
(Wiley, 2023) Ceylani, Taha; Teker, Hikmet Taner; Keskin, Seda; Samgane, Gizem; Acikgoz, Eda; Gurbanov, Rafig
This study aims to investigate the effects of plasma exchange on the biomolecular profiles and histology of ileum and colon tissues in young and aged Sprague-Dawley male rats. Fourier transform infrared (FTIR) spectroscopy, linear discriminant analysis and support vector machine (SVM) techniques were employed to analyse the lipid, protein, and nucleic acid indices in young and aged rats. Following the application of young plasma, aged rats demonstrated biomolecular profiles similar to those of their younger counterparts. Histopathological and immunohistochemical assessments showed that young plasma had a protective effect on the intestinal tissues of aged rats, increasing cell density and reducing inflammation. Additionally, the expression levels of key inflammatory mediators tumour necrosis factor-alpha and cyclooxygenase-2 significantly decreased after young plasma administration. These findings underscore the therapeutic potential of young plasma for mitigating age-related changes and inflammation in the intestinal tract. They highlight the critical role of plasma composition in the ageing process and suggest the need for further research to explore how different regions of the intestines respond to plasma exchange. Such understanding could facilitate the development of innovative therapies targeting the gastrointestinal system, enhancing overall health during ageing.
Role of the Probiotic Supplementation on Intestinal Inflammation and Structural Integrity in Wistar Rats Subjected To a Cafeteria Diet During Development
(Univ Agriculture, Fac veterinary Science, 2025) Onlu, Harun; Teker, Hikmet Taner; Keskin, Seda; Genc, Aysun Inan; Allahverdi, Huseyin; Elarslan, Aylin; Ceylani, Taha
Probiotics have gained significant interest in medical and veterinary sciences due to their potential to improve gastrointestinal health. This study investigates the protective role of probiotics on intestinal health in male Wistar rats exposed to a cafeteria diet during development. The experimental groups were divided into four: control, probiotics, cafeteria diet, and cafeteria diet with probiotics. Probiotics groups were administered daily at 1 x 108 CFU throughout the experiment. Ileum and colon tissues were analyzed via Fourier-transform infrared spectroscopy, histopathological analysis, and immunohistochemical staining. The cafeteria diet group showed altered lipid profiles, increased protein carbonylation (a marker of oxidative stress), and increased mast cell density, indicating increased intestinal inflammation. Probiotic supplementation significantly reduced inflammation by reducing TNF-alpha (P <= 0.0001) and IL-1(3 (P <= 0.0001). These results suggest that probiotic supplementation during an unhealthy diet can mitigate adverse effects by reducing oxidative stress and inflammation. Thus, probiotics could offer therapeutic potential in mitigating cafeteria diet-induced intestinal changes, serving as a promising dietary intervention during development to manage metabolic disorders in both humans and animals.
Scd Probiotics Mitigate Cafeteria Diet-Induced Liver Damage in Wistar Rats During Development
(Wiley, 2023) Ceylani, Taha; Onlu, Harun; Keskin, Seda; Allahverdi, Huseyin; Teker, Hikmet Taner
Background and AimThe liver plays a critical role in metabolic homeostasis, and its health is often compromised by poor dietary habits. This study aimed to investigate the therapeutic potential of SCD Probiotics in mitigating adverse liver effects induced by a cafeteria diet in male Wistar rats during their developmental period.MethodsFour groups of seven male Wistar rats each were subjected to different dietary regimens from day 21 (weaning) to day 56. The groups were as follows: a control group on normal feed; a probiotic-supplemented group on normal feed; a group on a cafeteria diet mixed with normal feed; and a group on a cafeteria diet mixed with normal feed, supplemented with SCD Probiotics. Liver health was assessed using Fourier transform infrared spectroscopy and histopathological evaluations.ResultsRats on the cafeteria diet exhibited significant disruptions in lipid, protein, cholesterol, triglyceride levels, and glycogen/phosphate content. Histopathological abnormalities such as lymphocytic infiltration, steatosis, and necrosis were also observed. However, SCD Probiotics supplementation led to notable improvements in the liver's biomolecular composition and mitigated histopathological abnormalities. Serum liver enzyme levels (AST, ALT, ALP, and LDH) also showed beneficial effects, while serum albumin levels remained stable.ConclusionsSCD Probiotics demonstrated a promising potential to counteract the adverse liver effects induced by a cafeteria diet in male Wistar rats. The study revealed significant improvements in biomolecular composition, histopathology, and serum enzyme levels. However, these findings are preliminary and necessitate further in vivo studies and clinical trials for validation. image
Supplementing Probiotics During Intermittent Fasting Proves More Effective in Restoring Ileum and Colon Tissues in Aged Rats
(Wiley, 2024) Teker, Hikmet Taner; Ceylani, Taha; Keskin, Seda; Samgane, Gizem; Allahverdi, Hueseyin; Acikgoz, Eda; Gurbanov, Rafig
This study aimed to explore the impact of SCD Probiotics supplementation on biomolecule profiles and histopathology of ileum and colon tissues during a 30-day intermittent fasting (IF) program. Male Sprague-Dawley rats, aged 24 months, underwent 18-h daily fasting and received 3 mL (1 x 108 CFU) of SCD Probiotics. The differences in biomolecule profiles were determined using FTIR Spectroscopy and two machine learning techniques, Linear Discriminant Analysis (LDA) and Support Vector Machine (SVM), which showed significant differences with high accuracy rates. Spectrochemical bands indicating alterations in lipid, protein and nucleic acid profiles in both tissues. The most notable changes were observed in the group subjected to both IF and SCD Probiotics, particularly in the colon. Both interventions, individually and in combination, decreased protein carbonylation levels. SCD Probiotics exerted a more substantial impact on membrane dynamics than IF alone. Additionally, both IF and SCD Probiotics were found to have protective effects on intestinal structure and stability by reducing mast cell density and levels of TNF-alpha and NF-kappa B expression in ileum and colon tissues, thus potentially mitigating age-related intestinal damage and inflammation. Furthermore, our results illustrated that while IF and SCD Probiotics individually instigate unique changes in ileum and colon tissues, their combined application yielded more substantial benefits. This study provides evidence for the synergistic potential of IF and SCD Probiotics in combating age-related intestinal alterations.
Therapeutic Potential of Young Plasma in Reversing Age-Related Liver Inflammation Via Modulation of Nlrp3 Inflammasome and Necroptosis
(Springer, 2025) Baba, Burcu; Ceylani, Taha; Teker, Hikmet Taner; Keskin, Seda; Genc, Aysun Inan; Gurbanov, Rafig; Acikgoz, Eda
The phenomenon of inflammaging, characterized by an increase in low-grade chronic inflammation, is closely associated with diseases related to liver dysfunction. This study investigated daily plasma exchange between 5-week-old and 24-month-old Sprague Dawley rats for 30 days, focusing on protein secondary structures, NLRP3 inflammasome, and necroptosis. Conformation changes in protein secondary structures were identified by infrared spectroscopy-based pattern recognition analysis. Liver biopsies with histochemical and immunohistochemical staining were used to assess molecules associated with inflammation, necroptosis and NLRP3 inflammasome complex. Expression levels of NLRP3 components were determined by qPCR. Enhanced random coils, 310 helices, beta-turns, and loop structures were identified in old rats and young rats with old plasma. Young rats and old rats with young plasma displayed higher alpha-helices and beta-sheet structures. Young rats with old plasma showed increased NLRP3, ASC, caspase-1, IL-1 beta, and IL-18 mRNA levels, indicating an inflammatory response. Whereas old rats with young plasma exhibited lower inflammation levels. Histological evaluations revealed that young rats receiving aged plasma showed significantly increased levels of NLRP3, ASC, caspase-1, IL-1 beta, TNF-alpha, VEGFR2, RIPK1, and MLKL immunoreactivity, whereas decreased immunoreactivity in aged rats receiving young plasma. These findings suggest that young plasma reduces NLRP3 inflammasome activation and necroptosis in aged rats.