Browsing by Author "Temel, Halide Edip"

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Chemical Profile, in Vitro Pharmacological Activity and Satureja Cuneifolia Ten. Evaluation of Essential Oil Based on Distillation Time
(Taylor & Francis Ltd, 2024) Yildiz, Gulsum; Ilgun, Selen; Karatoprak, Gokce Seker; Kose, Yavuz Bulent; Goger, Fatih; Temel, Halide Edip; Demirci, Betul
The medicinal plant Satureja cuneifolia Ten. was widely utilized as spice, tea and traditional medicine. The objective of the current study was to examine the chemical composition and in vitro biological activities (LOX, MMP-1, and MMP-12 enzyme inhibition activity and cytotoxicity on A549 cell line) of Satureja cuneifolia extracts and essential oils. The essential oils of the flowering aerial parts were hydro-distilled at four different distillation times (5, 30, 60, and 180 min) using the Clevenger apparatus. The total essential oil and four fragments were compared in terms of the major component, yield, and distillation time. Volatile compounds of the infusion were extracted by using HS-SPME. Ethanolic extract had the strongest inhibition activity on the LOX enzyme (84.50%), while the essential oils exhibited more cytotoxic activity on the A549 cell line than the extracts. The oils and the infusion were analyzed using GC-MS and the primary chemicals identified by LC-MS/MS.
Origanum Minutiflorum O. Schwarz Et P. H. Davis Essential Oil: Enzyme Inhibitory Activities and Chemical Composition
(Marmara Univ, 2023) Yildiz, Gulsum; Demirci, Betuel; Temel, Halide Edip; Kirimer, Nese
The essential oil (EO) of Origanum minutiflorum O. Schwarz et P. H. Davis was obtained by the hydrodistillation method. The analysis of the EO was conducted using Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS). The yield of the EO was determined to be 3.9%. Fifty-six compounds were identified, constituting 97.8% of the EO. The EO was rich in carvacrol at a rate of 83.3%, and the other major compounds were p-cymene (3.0%), beta-caryophyllene (1.3%), trans-sabinene hydrate (1.1%), gamma-terpinene (1.1%), borneol (1.1%), and terpinene-4-ol (1.0%). While the EO (100 mu g/mL) demonstrated the highest inhibition on cyclooxygenase-1 (COX-1) with 55.26%, the inhibitory activities on the other enzymes were as follows: cyclooxygenase-2 (COX-2); 33.10%, and matrix metalloproteinase-9 (MMP-9); 12.87%. The EO had no inhibition on lipoxygenase (LOX). In this research, inhibitory activity of the EO of O. minutiflorum on COXs and MMP-9 enzymes was reported for the first time.