Browsing by Author "Temel, Hamdi"

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A Detailed Chemical and Biological Investigation of Twelve Allium Species From Eastern Anatolia With Chemometric Studies
(Wiley-v C H verlag Gmbh, 2021) Izol, Ebubekir; Temel, Hamdi; Yilmaz, Mustafa Abdullah; Yener, Ismail; Olmez, Ozge Tokul; Kaplaner, Erhan; Ertas, Abdulselam
Allium species are widely consumed as food all over the world. The phenolic profile of ethanol extracts of aerial parts and roots of 12 Allium species, collected from five different Eastern Anatolia regions, were studied using LC-MS/MS. In vitro antioxidant, anticholinesterase, cytotoxic and antimicrobial activities were also tested. The multivariate analyses were performed using principal component and hierarchical cluster analyses. Seventeen of 27 standard compounds were detected in all Allium species. The major components were mainly identified as quinic acid, malic acid, vanillin, and p-coumaric acid. The aerial parts possessed better antioxidant activity than roots. Aerial parts of A. atroviolaceum, A. chrysantherum, A. kharputense, and A. shirnakiense exhibited high cytotoxic activity against DLD-1 colon cancer cell lines (IC50 12.5 mu g/mL). A. shatakiense and A. vineale demonstrated good antimicrobial activity against S. aureus and E. coli (MIC 75 mu g/mL). According to chemometric analysis, differences were detected between aerial parts and the roots. The aerial parts of A. atroviolaceum, A. chrysantherum, A. kharputense, and A. shirnakiense could be potent in the pharmaceutical industry while A. shatakiense and A. vineale in the food industry after further investigations.
A Detailed Study on Chemical and Biological Profile of Nine Euphorbia Species From Turkey With Chemometric Approach: Remarkable Cytotoxicity of E-Fistulasa and Promising Tannic Acid Content of E-Eriophora
(Elsevier Science Bv, 2018) Yener, Ismail; Olmez, Ozge Tokul; Ertas, Abdulselam; Yilmaz, Mustafa Abdullah; Firat, Mehmet; Kandemir, Sevgi Irtegun; Temel, Hamdi
The propose of this study is to investigate the chemical constituents of nine Euphorbia species; namely, E. aleppica, E. eriophora, E. macroclada, E. grisophylla, E. seguieriana subsp. seguieriana, E. craspedia, E. denticulata, E. falcata and E. fistulosa by LC-MS/MS as well as their antioxidant, anticholinesterase, tyrosinase inhibitory, urease inhibitory and cytotoxic activities. Among 59 extracts prepared from the different parts of the mentioned Euphorbia species, E. seguieriana subsp. seguieriana leaf (IC50: 10.41 +/- 0.93 mu g/mL) in lipid peroxidation inhibitory by beta-carotene-linoleic acid assay exhibited the highest activity, while E. grisophylla root in DPPH free radical scavenging (IC50: 0.79 +/- 0.01 mu g/mL), E. grisophylla seed in ABTS cation radical scavenging (IC50: 8.93 +/- 0.02 mu g/mL), E. fistulosa root both in acetyl- and butyryl-cholinesterase inhibitory (53.71 +/- 1.89% and 77.63 +/- 0.60% inhibition, respectively), E. denticulata leaf in urease inhibitory (96.89 +/- 2.00% inhibition), and E. macroclada mixed in tyrosinase inhibitory (86.99 +/- 3.77% inhibition) activities. However in cytotoxic activity studies, E. denticulata against PDF fibroblast cell lines (IC50: 23.04 +/- 0.03 mu g/mL), E. craspedia against HT-29 cancer cell line (IC50: 13.74 +/- 0.02 mu g/mL), E. fistulosa against MCF-7 and DLD-1 cancer cell lines (IC50: 14.04 +/- 0.04 mu g/mL and IC50: 20.23 +/- 0.08 mu g/mL, respectively) indicated quite good activity. According to the LC-MS/MS results, these species were rich in quinic acid, malic acid and tannic acid, rutin, hesperidin and hyperoside. Incidentally, it was found that the E. eriophora possessed very strong irritating potential. A chemometric approach using principal component analysis (PCA) and hierarchical clustering analysis (HCA) techniques were also studied on collected data to reveal the relationship between chemical contents of different parts of these Euphorbia species, and their biological activities, together with regional differences. Overall, these species could be suggested as valuable sources of natural-bioactive agents for developing new functional, pharmacological and health promoting ingredients.
Development and Validation of a Novel Lc-ms/Ms Method for the Quantitation of 19 Fingerprint Phytochemicals in Salvia Species: a Chemometric Approach
(Oxford Univ Press inc, 2022) Yilmaz, Mustafa Abdullah; Ertas, Abdulselam; Yener, Ismail; Olmez, Ozge Tokul; Firat, Mehmet; Temel, Hamdi; Kolak, Ufuk
Being traditionally utilized mainly as appetizers and herbal teas as well as used to ease abdominal pains, colds and gastrointestinal issues, the genus Salvia L. has gained significant consideration owing to its remarkable phytochemicals and industrial importance. The present study aimed to develop and validate an LC-MS/MS method for the qualitative and quantitative investigation of 19 fingerprint phytochemicals in six endemic Salvia species. The validation parameters of the developed LC-MS/MS method were repeatability (intermediate precision), recovery (accuracy), limits of detection and quantification, linearity and uncertainty (U% at 95% confidence level (k = 2)). Reversed-phase HPLC separation and mass spectrometry parameters were optimized for each analyte. Ethanol extracts of the studied Salvia species collected in three consecutive years were screened for their fingerprint phytochemicals by using the developed and validated LC-MS/MS method. Moreover, studied Salvia species were subjected to multivariate analysis such as principal component analysis techniques to demonstrate the variabilities in phytochemical contents by years and parts of the samples. Roots, flowers, leaves, branches and whole plant of the Salvia species collected in 2015, 2016 and 2017 were used for the analyses. It was observed that the roots and branches of Salvia species were similar in terms of their salvianolic acid A, caffeic acid, and 6,7-dehydroroyleanone components. Accordingly, apigenin, rosmarinic acid, luteolin 7-O-glucoside, caffeic acid, salvianolic acid B, and 6,7-dehydroroyleanone were notable phytochemicals that were present in the studied Salvia species.
Trace Element Analysis by Icp-Ms and Chemometric Approach in Some Euphorbia Species: Potential To Become a Biomonitor
(Shaheed Beheshti Univ, Sch Pharmacy, 2019) Yener, Ismail; Temel, Hamdi; Tokul-Olmez, Ozge; Firat, Mehmet; Oral, Elif Varhan; Akdeniz, Mehmet; Ertas, Abdulselam
In this study, the branch, leaves, flowers, roots and mixed parts of different nine Euphorbia species were analyzed for their trace element contents by using ICP-MS. The samples were digested by concentrated nitric acid and hydrogen peroxide in a microwave by ICP-MS before the analysis. The accuracy and precision of the method was evaluated by CRM 1573a Tomato Leaves. Trace element contents accumulated in different parts of each sample were contrasted. Minitab Statistical Software Inc., programme was used for the multivariate analysis of 12 toxic metals of seeds, roots, branches, leaves, flowers, and mixed parts of Euphorbia species collected from Diyarbakir, Kayseri, Malatya, Mardi'', Trabzon, and Van cities.When the studied Euphorbia species are compared in terms of their metal contents; V, Tl, Cr, and Ni metals in E. eriophora, Ba in E. aleppica, As and Co metals in E. segtieriana, Ag and Se metals in E. craspedia, Cu and Cd metals in E. fistulosa, Cs and Pb metals in E. grisophylla, Zn in E. macroclada and also Rb and Sr metals in E. denticulata were determined higher. It was determined that the studied species accumulated some metals at highly amounts especially in the root and leaf parts. In general, it can be said that Euphorbia species have high potential to become a biomonitor. For this reason, it can be predicted that these species will be used as ornamental plants in landscape architecture due to both their toxic metals retention properties and their beautiful appearance.