Browsing by Author "Temiz, M.A."
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Article Effect of Solvent Variation on Polyphenolic Profile and Total Phenolic Content of Olive Leaf Extract(Centenary University, 2017) Temiz, M.A.; Temur, A.Olive tree is one of the oldest plants, evergreen and of which fruits, leaves and trunk can be used for many different purposes. Olive has become a nutritional source widely used for especially in the Mediterranean diet and due to its extensive metabolic effects attracting everyone's attention recently. Olive and olive leaves are rich in polyphenolic compounds. Oleuropein is the most available and most active polyphenolic compound. Hydroxytyrosol, tyrosol and verbascoside are other important active compounds. Oleuropein has antioxidant, antidiabetic and antimicrobial effects. This study was investigated the effects of solvent variation on the amount of polyphenolic compounds and total phenolic content in olive leaves. The olive leaves were extracted using water, methanol/water and methanol solvents. The content of olive leaves extract was investigated by chromatographic analysis. Also total phenolic content was determined using Folin-Ciocalteu method by spectrophotometric method. The chromatographic analysis showed that methanolic solvents more effectively extracted oleuropein from olive leaf. However, when water was used as a solvent, hydroxytyrosol, tyrosol and verbascoside were extracted better from olive leaf. When solvents were compared in respect to their total phenolic content; the order from highest to lower was respectively methanol, methanol/water, and water. Consequently, due to their high hydrophilic character; polyphenolic compounds can more efficiently be extracted in methanolic solvents with high polarity. © 2017, Centenary University. All rights reserved.Article The Effects of Melatonin and Vitamin E on Antioxidant Parameters in Copper Nephrotoxicity(Ataturk Universitesi, 2018) Temiz, M.A.; Temur, A.Copper (Cu) compounds have been widely used in various fields especially industrial processes and agriculture. Although trace amounts of Cu are essential to sustain life, excessive Cu is toxic for living organisms. This study aims to investigate the effects of vitamin E and melatonin on antioxidant enzymes activities in kidney against acute Cu toxicity. In this study, 28 Wistar albino rats were divided four equal groups (n=7) as control, Cu, Cu+Vitamin E, and Cu+Melatonin groups. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) enzymes activities and protein carbonyl content were analyzed. Also, kidney function tests were evaluated. While Cu administration increased creatinine, blood urea nitrogen (BUN), uric acid, Na, K and Mg levels, vitamin E and melatonin was effective reduction on these levels. Carbonyl level significantly decreased in Cu+Vitamin E and Cu+melatonin groups compared to Cu group. Although SOD, GPx and CAT activities in tissue decreased by Cu exposure, vitamin E and melatonin treatment showed significant increase on their activities. Consequently, vitamin E and melatonin supplements may provide prevention Cu -based oxidation by strengthening the antioxidant defense system. In this respect, it may show a protective effect by preventing the development of nephrotoxicity. © Ataturk Universitesi. All rights reserved.Article Mixture of Leaf and Flower Extract of Prunus Spinosa L. Alleviates Hyperglycemia and Oxidative Stress in Streptozotocin-Induced Diabetic Rats(Elsevier B.V., 2021) Temiz, M.A.; Okumus, E.; Yaman, T.; Keles, O.F.Diabetes Mellitus is a global health problem that leads to various complications associated with hyperglycemia. In traditional medicine, herbal treatment is one of the alternative ways to cope with this type of disease. The aim of this study is to investigate the antidiabetic and hepato-pancreatic protective effects of the mixture of Prunus spinosa leaves and flowers (PSE) extract in streptozocin-induced diabetes mellitus. Seven random experimental groups of Wistar rats (n = 8) were created as followed; control, diabetic, PSE25, PSE50, insulin, metformin, and acarbose. α-amylase and α-glucosidase inhibitory activities of PSE were determined. Antioxidant enzymes activities and lipid peroxidation were analyzed in the liver tissue. Histopathological examination of liver and pancreas was also performed. α-amylase and α-glucosidase IC50 inhibition values of PSE were found more efficient, comparing to those of standard acarbose. While blood glucose levels severely increased in all diabetic groups, PSE25 and PSE50 treatments were effective in regulating blood glucose levels. Moreover, administration of PSE25 and PSE50 improved insulin levels compared to the diabetic group. Although increased oxidative stress in the diabetes seriously suppressed antioxidant activities, PSE25 and PSE50 supplementation significantly recuperated liver antioxidant capacity. Despite severe degenerative and necrotic changes in diabetes, these findings alleviated with PSE administrations. Moreover, PSE treatments remarkably recuperated β-cells. These results reveal that there may be an alternative way to control high blood glucose levels, which is one of the most important complications of diabetes. Furthermore, PSE can provide a protection against oxidative stress, liver and pancreatic damage by augmenting antioxidant capacity in diabetes. © 2021 SAABArticle Morphological and Functional Changes in Rat Brain Under Total Sleep Deprivation(Istanbul University, 2015) Abushov, B.; Temur, A.; Temiz, M.A.In this article the effect of 36 h total sleep deprivation (TSD) on behavioural reactions of rats (rearing, grooming and sexual activity) and on ultrastructure of brain neurons have been studied. A group of somnogenic structures (III-V layers of the frontal limbic cortex, CA1 area of the dorsal hippocampus, reticular formation of pons varolli, nucleus raphe dorsalis and locus coeruleus) of the brain of 6 month-old Wistar rats has been analized in this study. It has been revealed that dystrophic changes (chromatolysis of cytoplasm and vacuolization) enveloping a group of medium-sized neurons (20-30 (μ) micron in diameter) are accompanied by decrease of numbers of rearing, grooming and sexual activity. It is supposed that dystrophic changes revealed in medium-sized neurons result in partial disturbances of integrative and behavioral reactions of animals.