Browsing by Author "Temur, A"

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A Biochemical and Histochemical Study on the Activity of Acid Peptide Hydrolase in the Hypothalamus at Some Periods of Starvation and Refeeding After Starvation
(Mrs Pushpa Agarwal, 2005) Temur, A; Karadag, H; Askerov, FR; Dönmez, HH
This study was carried out to determine acid peptide hydrolyse activity in the homogenate of the lateral (feeding centre) and ventro-medial (satiety centre) nuclei of hypothalamus and fractions of the cells (mitochondria, nucleus, cystocoele) in those centres of rats fed after being starved for a five day period. One hundred and sixty Wistar Albino rats were used in this study. The rats were starved for periods of 1, 3, 5 and 7 d. The rats starved for 5 d were then allowed to cat for periods of 5, 15 and 30 d. The activity of acid peptide hydrolyse was biochemically determined in the feeding and satiety centres. In addition, histochemical study was also done in these centres. It was observed that the activity of acid peptide hydrolyse became closer to the levels of the control group in the satiety centre on the 30th d of access to food after a 5 d starvation. It was concluded that the feeding centre was more sensitive to starvation than the satiety centre and the satiety centre was restored faster than the feeding centre.
Dynamics of Changes of Monaminoxidase Contents in Some Hypothalamic Nuclei After Different Periods of Food Deprivation and Restoration of Food Regime
(Mrs Pushpa Agarwal, 2005) Temur, A; Askerov, FB; Kadimova, SO; Karadag, H
Monaminoxidase contents were determined by quantitative cytophotometric method in neuronal organization of lateral nucleus, ventromedial nucleus, paraventricular nucleus and supraoptic nucleus of hypothalamus after different periods of food deprivation and restoration of food regime. Monoaminergic system of laternal nucleus is probably necessary for keeping of informational and energetic potentials of the neuronal organizations of laternal nucleus and also for supporting of functional connections with all levels of food functional system. The neurotransmitter system of ventro-medical nucleus is necessary for gradual realization of energetic sources of the organism.
Immunohistochemical Detection of Peste Des Petits Ruminants Viral Antigens in Tissues From Cases of Naturally Occurring Pneumonia in Goats
(Elsevier Science Bv, 2004) Yener, Z; Saglam, YS; Temur, A; Keles, H
Peste des petits ruminants (PPR) is an important viral disease of goats and sheep characterized by erosive stomatitis, enteritis, and pneumonia. But the pneumonia is the most frequently recorded lesion in PPR infections. In this study, the presence of PPR viral antigens in goat's pneumonia was investigated. For this purpose, a total of 1505 goat's lungs slaughtered in Bitlis and Van slaughterhouses were grossly examined and pneumonia was detected in 74 cases (4.91%). Lesions were more frequently encountered in anteroventral lobes than caudal lobes. Immunohistochemical (IHC) examinations were performed on 42 pneumonic lungs (except the verminous pneumonia). IHC staining of formalin-fixed, paraffin-embedded tissues were carried out by the avidin-biotin-peroxidase complex (ABC) procedure. The presence of PPR viral antigens were detected in 17 (40%) out of 42 pneumonic lungs. Viral antigens were found most frequently in the cytoplasm and rarely in the nucleus, including bronchial glands, bronchial and bronchiolar epithelial cells, type II pneumocytes, syncytial cells, and alveolar macrophages. In conclusion, it was found that there was a close relationship between the pneumonia in goats and the presence of PPR viral antigens. The PPR incidence in pneumonic lungs of goats was detected to be very high in the present study performed in the region of Bitlis and Van. (C) 2003 Elsevier B.V. All rights reserved.
Polyphenol Oxidase From Allium Sp
(Amer Chemical Soc, 1997) Arslan, O; Temur, A; Tozlu, I
Polyphenol oxidase (PPO) was isolated from Allium sp. PPO showed activity to catechol and DL-dopa (K-m values were 25 mM for cathecol and 33 mM for DL-dopa; V-max values were 666 EU/mL . min for cathecol and 166 EU/mL . min for DL-dopa). Catechol was the most suitable substrate for Allium sp. PPO (lowest K-m value). The optimum pH for the PPO was 7.5 on substrates catechol and DL-dopa. Heat inactivation studies showed temperature >40 degrees C resulted in loss of enzyme activity. Heating for 30 min at 40 degrees C did not cause a significant loss of enzymatic activity. Allium sp. PPO was significantly inhibited in the presence of ascorbic acid, 2-mercaptoethanol, and sodium metabisulfide.
Polyphenol Oxidase From Malatya Apricot (Prunus Armeniaca L.)
(Amer Chemical Soc, 1998) Arslan, O; Temur, A; Tozlu, I
Polyphenol oxidase (PPO) of Malatya apricot was isolated by (NH4)(2)SO4 precipitation and dialysis. PPO showed activity to catechol, L-dopa, and gallic acid. Catechol was the most suitable substrate for Malatya apricot PPO (lowest K-m value). The optimum pH for the PPO was 8.5. Heating for 40 min at 40 degrees C did not cause a significant loss of enzymic activity. The times required for 50% inactivation of activitiy at 60 and 80 degrees C were found to be 47 and 16 min, respectively. The I-50 values of inhibitors studied on PPO were determined by means of activity percentage [I] diagrams. The values were 2.7 x 10(-5), 5.03 x 10(-5), 3.62 x 10(-5), 3.68 x 10(-5), and 8.30 x 10(-4) M for sodium metabisulfite, ascorbic acid, 2-mercaptoethanol, thiourea, and salicylic acid, respectively. Ascorbic acid, 2-mercaptoethanol, sodium metabisulfite, and thiourea inhibited the reaction strongly.