Browsing by Author "Testereci, H."

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Dietary Nitrate and Nitrite Levels in an Endemic Upper Gastrointestinal (Esophageal and Gastric) Cancer Region of Turkey
(2003) Türkdoǧan, M.K.; Testereci, H.; Akman, N.; Kahraman, T.; Kara, K.; Tuncer, I.; Uygan, I.
Background/aims: The aim of this study was investigate nitrate and nitrite levels in some traditional foods and drinking water in Van, an endemic upper gastrointestinal (esophageal and gastric) cancer region of Eastern Turkey. Methods: Cheese with added herbs, drinking; water, bread baked by wood fire and bread baked by burning animal manure specimens collected in the Van region were analyzed by Stahr's modification method. Nitrite was reduced to nitrate by the following three reagents: hydrazine sulphate ((NH2) 2SO4), CuSO4 and NaOH. Nitrate was then determined by coupling reagent containing sulphanlyamide, phosphoric acid and N-1-naphtyl ethylendiamine dihydrochloride. Results: Nitrate and nitrite levels of all traditional foods and nitrite levels of drinking water were very significantly elevated compared to standard values (p<0.001-0.01). Conclusions: These findings suggest that the influence of a traditional diet rich in nitrate and nitrite is significant in the development of endemic upper gastrointestinal (esophageal and gastric) cancers in the Van region of Turkey.
Nitric Oxide Oxidation Products in the Serum of Patients With Gastric and Esophageal Cancer and Helicobacter Pylori
(1999) Turkdogan, M.K.; Testereci, H.; Kahraman, T.; Dilek, F.H.; Akman, E.
Nitric oxide (NO) oxidation products (nitrate and nitrite) were analysed in the serum of 12 gastric cancer (Ca), (12 gastric Ca, 12 esophageal Ca) and 12 healthy controls with modified Stahr's method. Helicobacter pylori (H. pylori) infection was diagnosed histopathologically in the gastric cancer and control groups. In the gastric Ca group mean, serum nitrate and nitrite levels (11.65 ppm and 3.40 ppm) were significantly higher than the esophageal Ca and control groups (p<0.05 and p<0.001). In the esophageal Ca group the mean serum nitrate level (7.16 ppm) was higher than the control group (4.73 ppm, p<0.05) while the nitrite level (1.53 ppm) was not significantly different from the control group (1.70 ppm). H. pylori infection was significantly higher in the gastric Ca group (56.40%) than the control group (36.66%, p<0.05). In conclusion, we observed the highest serum NO product levels in the gastric Ca group although the dietary intake of nitrated foods was similar in both of the Ca groups. This observation indicates the important role of H. pylori infection in the etiopathogenesis of gastric cancer related to the synthesis of NO and consequent the carcinogen nitroso compounds.
Separation of Retinol Acetate, Vitamin a Palmitate, Α- and Γ- Tocopherol by Reverse-Phase High Performance Liquid Chromatography and Determination of These Vitamins in Van Lake Fish (Chalcalburnus Tarichi)
(Scientific and Technical Research Council of Turkey, 1997) Testereci, H.; Yörük, I.H.; Akyüz, A.; Saǧmanligil, H.
The resolution properties of several commercial columns for vitamin A and E standards by reverse phase chromatography methods have been examined. Hypersil MOS column with methanol-water (96:4) and acetonitrile-water (90:10) mobile phase, Shimadzu-ODS column with methanol-water (96:4) mobile phase have acceptable resoluion in separation of vitamin A esters. Shimadzu-ODS column/96 % methanol and Shimadzu-C8/90% acetonitrile have the acceptable resolution ratio for vitamin E isomers. Methanol extracts of heart muscle and gill in Van Lake fish (Chalcalburnus tarichi) have been examined for vitamin A and E by high performance liquid chromatography. Avarage retinol acetate, vitamin A palmitate, alpha tocopherol, gamma tocopherol in heart muscle of Van Lake fish, have been found as 162±23 ng/g, 18±2.2 ng/g, 8.5±1.0 μg/g, 505.7±94 ng/g, respectively. The vitamin levels in gills have been determined as retinol acetate 231.5±38 ng/g, vitamin A palmitate 23.8±3.5 ng/g, alpha tocopherol 12.4±0.77 μg/g, gamma tocopherol 350.1±97 ng/g. A significant differences have been found among the retinol acetate-vitamin A patmitate and alpha-gamma tocopherol values in heart muscles and gills (P<0.05). A negative correlation for retinol acetate between heart muscle and gill (r:-.86), vitamin A palmitate between heart muscle and gill (r:-.73) and between retinol acetate-alpla tocopherol in heart muscles (r:-.71) have been observed. A positiv correlation between retinol acetate and alpha tocopherol in gills (r:+.52) has been found. No correlation has seen between heart muscles and gills for gamma tocopherol values.
Serum and Tissue Levels of Vitamin a and E in Gastric and Esophageal Cancer
(1996) Turkdogan, M.K.; Testereci, H.; Kahraman, T.; Tuncer, I.; Algun, E.; Yoruk, I.H.
Vitamin A and Vitamin E have antioxidant properties and their role in the prevention of gastrointestinal cancers by way of scavenging lipid peroxidation products is very important. Vitamin A ester (retinol acetate) and vitamin E isomers (alpha, gamma and total tocopherols) have been investigated in the serum samples and tissue biopsies of 7 esophageal, 12 gastric cancer patients and a control group consisting of 12 healthy subjects. The serum mean retinol acetate level was significantly higher in the gastric cancer group but not in patients with esophageal cancer when compared to the control group. Serum mean tocopherol levels were low in both cancer groups when compared to the control group but this did not reach biological significance. Tissue mean retinol acetate and tocopherol levels were found significantly low in both of the cancer groups (p<0.03 and p<0.08). These findings suggest that the low tissue levels of the antioxidant vitamin A and E may be related to the etiopathogenesis of the gastrointestinal cancers.
Therapeutic Efficacy of Doramectin as a New Endectocid on Natural Scabe and Trichostrongylose and Changes for Some Blood Parameter of Akkaraman Sheep in Van
(Scientific and Technical Research Council of Turkey, 1996) Sekin, S.; Voyvoda, H.; Testereci, H.
Two studies were conducted to evaluate the endectocidal therapeutic efficacy of Doramectin on Akkaraman Sheep naturally infested with S. ovis and P. ovis in Van. PCV, Hb and TP values were evaluated for infestation status. Doramectin (1%) was administered to sarcoptic sheep as a single 200 μg/kg dosage and to psoroptic sheep as a single 300 μg/kg dosage. The control groups received a single equivalent dosage of NaCl (0.9%). The complete recovery of clinical signs on sarcoptic sheep was observed in the third week. While initial improvement of the clinical sings of psoroptic sheep was more apparent in the second week, total recovery was seen on the fourth week. In the first week 53.3% and in he second week 93.3% of the sheep tested negative for the S. ovis mite. Similary P. ovis mites had disappeared in 43.3% of the animals in the first week and in 86.7% in the second week after treatment. Three weeks after Doramection application, sarcoptic and psoroptic mites had been completely eliminated for the sheep. Doramection was also found to be 97.9% effective on nematoe eggs of the Thrichostrongylidae family (P<0.001). There were significant (P<0.001) increases in PCV and Hb, and a decrease (P<0.05 - 0.01) in TP for sarcoptic and psoroptic sheep on the 27th day of treatment. All these differences were found significant as comparied to the control groups.