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Browsing by Author "Timurkan, H"

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    Micro-Spoon Technique in Estimation of Sperm Concentration in Rat Epididymis
    (Tohoku Univ Medical Press, 2000) Odabas, Ö; Timurkan, H; Atilla, MK; Yilmaz, Y; Sengül, E; Aydin, S
    Several technical procedures mere described for estimation of epididymal sperm concentration until today. In all of the described techniques, epididymal material was drawn into a micro-pipette before counting procedure. We aimed to proceed this stage without using any pipette because of the difficulty in aspirating very viscous epididymal material into a micro-pipette. For measuring epididymal material we used a 2 mu l reservoir which me named as a micro-spoon. It was filled with the epididymal material and layered in a tube with Ringer's solution of 2 mi containing sodium hyaluronate. Then, it was mixed in order to obtain 1/1000 diluted suspension. Sperm cells in this suspension were counted on a Makler chamber under a light microscope. The procedure was easy and time saving, compared to the other techniques. (C) 2000 Tohoku University Medical Press.