Browsing by Author "Tonk, Fatma Aykut"

Now showing 1 - 4 of 4

Bazı Makarnalık Buğday Çeşitleri ile Yeni Geliştirilen Hatlarda Genetik İlişkilerin Rapd Markörleriyle İncelenmesi
(2008) Tonk, Fatma Aykut; Akçalı, R. Refika; Furan, M. Alp; Yüce, Süer
Ege Bölgesi’nde verim yönünden ön plana çıkan dört makarnalık buğday çeşidi ile uzun yıllar yapılan denemeler sonucu ümitvar görülen CIMMYT kökenli üç introdüksiyon hattında, moleküler markör yöntemlerinden RAPD metodu ile söz konusu genotipler arasında genetik olarak benzerliklerin ve farklılıkların incelenmesi bu çalışmanın amacını oluşturmuştur.Yapılan DNA analizleri sonucunda, çalışmada incelenen çeşit ve hatların genetik olarak birbirine yakın olduğu ve bunlara ait ortalama genetik benzerlik oranının 0.86 olduğu saptanmıştır. Ayrıca Ege-88 ile Yavaros-79 çeşitlerinin birbirlerine en yakın genotipler olduğu anlaşılmıştır.
Characterization of Various Hypericum Perforatum Clones by Hypericin and Rapd Analyses
(Friends Science Publ, 2011) Tonk, Fatma Aykut; Giachino, R. Refika Akcali; Sonmez, Cigdem; Yuce, Suer; Bayram, Emine; Telci, Isa; Furan, M. Alp
Hypericum perforatum L. is a pharmaceutically important medicinal plant with antidepressant, antiviral, antibacterial and anticancer effects of its metabolites. The hypericin contents and genetic structures of superior H. perforatum clones were identified by spectrophotometric analysis and random amplified polymorphic DNA (RAPD) markers, respectively. At the first harvest the hypericin content of the clones was higher than second harvest and higher in 2007 compared to 2008. For initial screening, 38 random primers were used and 26 of them detected a total of 378 scorable fragments. Effective multiplex ratio (EMR), average heterozygosity (H-av), marker index (MI), resolving power (RP) and polymorphic information content (PIC) of the primers were calculated for RAPD marker system. Genetic similarity value among the clones varied from 0.40 to 0.93 and the average genetic similarity values was determined as 0.60. Clustering based on RAPD data showed a partial relationship on a regional basis in the clones. Cluster analyses of hypericin contents and RAPD markers grouped the clones in two major clusters and significant correlations were observed between them. In conclusion, combined analysis of RAPD markers and hypericin content is an optimal approach for further progress and breeding programs. (C) 2011 Friends Science Publishers
Chemical and Genetic Variability of Selected Turkish Oregano (Origanum Onites L.) Clones
(Springer Wien, 2010) Tonk, Fatma Aykut; Yuce, Sueer; Bayram, Emine; Giachino, R. Refika Akcali; Sonmez, Cigdem; Telci, Isa; Furan, Mehmet Alp
Turkish oregano (Origanum onites L.) is a major aromatic plant that belongs to Labiatae family. In this work fourteen Turkish oregano clones have been characterized chemically and genetically. Essential oil obtained by hydro-distillation was characterized by gas chromatography. Genetic variation was determined by use of random amplified polymorphic DNA (RAPD) markers. Variation of essential oils in the selected clones was subjected to cluster analysis, and two chemotypes, carvacrol and thymol, were identified. Carvacrol was found to be the main component in all clones except clone-661, the main component of which was thymol. The oregano clones were divided into three main groups by clustering on the basis of RAPD markers. Genetic similarity values among the oregano clones ranged between 0.49 and 0.73 which was indicative of a low level of genetic variation. Clones originating from locations close to each other had similar RAPD markers. Correlation analysis of the genetic distance matrix and the Euclidian distance matrix revealed no significant correlation between them. The results also indicated that there is no relationship between genetic structure of the selected clones and essential oil composition.
Rapd and Essential Oil Characterization of Turkish Basil (Ocimum Basilicum L.)
(Springer Wien, 2014) Giachino, R. Refika Akcali; Sonmez, Cigdem; Tonk, Fatma Aykut; Bayram, Emine; Yuce, Suer; Telci, Isa; Furan, Mehmet Alp
Sweet basil (Ocimum basilicum L., Lamiaceae), an important medicinal plant and culinary herb due to its delicate aroma and fragrance, shows great variation in both morphology and essential oil components. Genetic variation among basil accessions in Turkey has not been extensively examined with molecular markers. Genetic diversity was determined using random amplified polymorphic DNA (RAPD) markers of 14 genotypes of basil. A total of 375 bands were obtained from the RAPD analysis, and 273 of them (70.3 %) were polymorphic. The RAPD analysis allowed the grouping of samples into two main clusters. Genetic similarity values among the basil genotypes ranged between 0.46 and 0.87. Considerable genetic diversity was determined among basil genotypes. Essential oils were obtained by hydro-distillation and were characterized by gas chromatography. A total of 17 chemical components were identified. The evaluated genotypes of O. basilicum can be classified into seven chemotypes: (1) Linalool (7, 12, 16, 22, 25A and 33), (2) Methyl chavicol (6, 10A), (3) Citral/methyl chavicol (10L, 17), (4) Methyl eugenol (11), (5) Methyl cinnamate/linalool (23), (6) Linalool/methyl eugenol (25K), and (7) Methyl chavicol/linalool (Let). The chemical variability obtained from the essential oil composition of the genotypes in the study was remarkable. The chemical characterization of genotypes 10L and 17 was rich in citral (42.17 and 44.80 %) and methyl chavicol (30.56 and 32.03 %). Citral/methyl chavicol can be assessed as a new chemotype of basil cultivated in Turkey. The basil genotypes were grouped into two major clusters for both the RAPD analysis and chemical characterization with very few exceptions (genotype n. 6). A correlation analysis of the genetic distance matrix and the Euclidian distance matrix showed relatively low values (r = -0.40). The results demonstrated a certain degree of correspondence between chemical and molecular data.