Browsing by Author "Tuncer, Burcu"

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Development of an Efficient in Vitro Callus Proliferation Protocol for Edible Wild Rhubarb (Rheum Ribes L.)
(Univ Life Sciences Lublin, 2021) Tuncer, Burcu
Rheum ribes L. is a perennial wild species. Young shoots and flower bunches are freshly consumed, and root and rhizomes are generally used for medicinal purposes. The aim of the present study was to improve the callus proliferation protocol for R. ribes L. under in vitro conditions. For callus induction, hypocotyl explants taken from 14-day old plantlets germinated in Murashige and Skoog (MS) media were cultured in MS media with 9 plant growth regulator (PGR) combinations containing 6-benzylaminopurine (BAP) (2, 3, and 4 mg/L) + naphthylacetic acid (NAA) (0.1, 0.5, and 1 mg/L). Then, for callus proliferation, 4 PGR combinations con-taining NAA (0.2 mg/L) + thidiazuron (TDZ) (0.5, 1, 2, and 3 mg) were used in the first set of experiments, and 36 PGR combinations containing BAP (1, 2, 3, and 4 mg/L) + indole-3-butyric acid (IBA) (0.2, 0.5, and 1 mg/L), BAP (1, 2, 3, and 4 mg/L) + NAA (0.2, 0.5, and 1 mg/L), and TDZ (1, 2, 3, and 4 mg/L) + NAA (0.2, 0.5, and 1 mg/L) were used in the second set of experiments. At the end of the second set of experiments, the greatest callus regeneration ratios were obtained due to the combinations including BAP and IBA as well as the low-dose TDZ-(especially 1 mg/L) and NAA-(0.2, 0.5, 1 mg/L) combinations. Regarding callus fresh weights, TDZ + NAA combinations were found to be more successful. The greatest callus fresh weight (12.7 +/- 0.4 g) was obtained from MS medium supplemented with 2 mg/L TDZ and 0.2 mg/L NAA.
Different Combination Treaments To Improve Germination and Emergence Performance on Seeds of Eremurus Spectabilis M.bieb.
(2019) Akdağ, Şeref; Tuncer, Burcu
Araştırmada, çiriş (Eremurus spectabilis) tohumlarının çimlenme ve çıkış performansını artırmaya yönelik farklı uygulamalar yapılmıştır. Bu amaçla tohumlara farklı sürelerde (30, 50, 80 ve 100 gün) soğukta (4ºC) nemli katlama uygulaması ile birlikte, gibberellik asit (GA3) (500 ve 750 ppm - 24 ve 48 saat), potasyum nitrat (KNO3) (5 mM - 24 ve 48 saat), kalsiyum klorür (CaCl2) (5 mM - 24 ve 48 saat), farklı besin ortamlarında (MS, B5 ve WH) in vitro çimlendirme uygulamaları ile tohum ucu kesme uygulamaları yapılmıştır. 24 saat süreyle GA3 uygulamalarında, en yüksek çimlenme ve çıkış oranı % 21.04 oran ile 500 ppm GA3 dozunda 100 gün katlama uygulamasından elde edilmiştir. 48 saat süreyle GA3 uygulamalarının etkisinde ise; çimlenme ve çıkış oranı değerlerinde GA3 dozu etkili bulunmazken, en yüksek çimlenme oranı, 500 ppm (% 20.36) ve 750 ppm (% 20.00) dozlarında 100 gün katlama uygulamasından, en iyi çıkışlar ise 500 ppm GA3 + 100 gün katlama (% 18.51) uygulamasından sağlanmıştır. 5 mM KNO3 uygulamalarında; çimlenme (%11.12) ve çıkış (%9.71) oranı, kökçük (0.29 cm), sapçık (3.00 cm) ve fide uzunluğu (3.29 cm) ile güç indeksinde 100 gün katlama süresinde 48 saat KNO3 uygulamaları ortalaması, 5 mM CaCl2 uygulamalarında ise; çimlenme (% 10.17) ve çıkış (% 8.37) oranı yönünden 24 saat CaCl2 uygulamaları ortalaması daha başarılı bulunmuştur. In vitro çimlendirme denemelerinden umutvar sonuçlar elde edilemezken, tohum ucu kesme uygulamaları tüm uygulamalar arasında en başarılı uygulama olarak tespit edilmiştir. Anahtar kelimeler: CaCl2, Eremurus spectabilis, KNO3, Soğukta nemli katlama, Tohum.
Dormancy Breaking Treatments for Wild Eremurus Spectabilis M.bieb Seeds
(Parlar Scientific Publications (p S P), 2019) Keskiner, Kader; Tuncer, Burcu
Eremurus spectabilis M.Bieb seeds were subjected to moist-cold (4 degrees C) stratifications for different periods (0, 30, 40, 50, 60, 70, 80, 90, and 100 days), gibberellic acid (GA3) (0, 250, 500, 750, 1000, 1500, and 2000 ppm), potassium nitrate (KNO3) (0, 5 mM, 10mM, and 15mM), calcium chloride (CaCl2) (0, 5 mM, 10 mM, and 15 mM) treatments and in vitro germinations in different nutrient media (MS, B5, and WH) to break seed dormancy. Treated seeds were germinated at 15 degrees C under two different photoperiod regimes (continuous dark and 16/8 hours light/dark). The greatest rate of germination was obtained from a combination of 100-day moist-cold stratification + continuous dark photoperiod (73.30%). For mean germination rates, the 5 mM dose of KNO3 (10.00%) and CaCl2 (3.33%) treatments yielded better outcomes than the other doses. The same treatments were also more successful in emergence parameters. With regard to germination and emergence parameters, positive outcomes were not able to be achieved from in vitro germination treatments in different nutrient media and GA(3) treatments.
Effect of Heat Shock Treatment on Microspore Embryogenesis in Brassica Oleracea Species
(Ankara Univ, Fac Agr, 2016) Tuncer, Burcu; Cig, Arzu; Yanmaz, Ruhsar; Yasar, Fikret
Heat shock treatments are widely used to induce microspore embryogenesis in Brassica species. In this study, the effect of high temperature treatment (32 degrees C and 35 degrees C for 2 days) on microspore embryogenesis was investigated in six genotypes of Turkish white head cabbage (Yalova-1, Ercis, 177 C, 177 T, 531 C, 538 C), three genotypes of Turkish kale (Balkaya, Yanmaz, Karadere 077) and five commercial F-1 ornamental kale hybrids (Red Piegon, Victoria Piegon, Red Chidori, white Kamome, and Pink Kamome). Microspore-derived embryos formation differed depending on genotype and high temperature. The highest embryo yield was obtained as 9.92 embryo per petri dish in cv. Yalova-1, 11.13 embryo per petri dish in Pink Kamome F-1 at 32 degrees C, and 5.63 embryo per petri dish in cv. Karadere 077 at 35 degrees C.
Effects of Colchicine and High Temperature Treatments on Isolated Microspore Culture in Various Cabbage (Brassica Oleraceae) Types
(Friends Science Publ, 2011) Tuncer, Burcu; Yanmaz, Ruhsar
The effect of colchicine with heat shock treatments on the microspore embryogenesis were evaluated in white head cabbage (B. oleracea var. capitata subvar. alba), kale (B. oleracea var. acephala) and ornamental kale (B. oleracea var. acephala). 2 different colchicine doses (50 & 100 mg/L) applied to the isolated microspores in order to stimulate the embryo induction for 15 h. After colchicine treatments microspores were exposed to 32 degrees C and 35 degrees C temperature in NLN-13 medium (40,000 microspore/mL) for 48 h. According to the 12 day and 19 day embryo yields after planting 50 mg/L colchicine treatment was found more effective. 32 degrees C+50 mg/L colchicine treatment (5.3 embryo/petri) in white head cabbage (Yalova-1) and 35 degrees C+50 mg/L colchicine treatment (9.4 embryo/petri) in ornamental kale were found effective treatments, respectively. White head cabbage (Er is genotype) and kale did not give positive respond to the colchicine treatments. (C) 2011 Friends Science Publishers
Effects of Combined Pretreatments on Germination and Emergence of Wild Eremurus Spectabilis M. Bieb. Seeds
(Univ Agr Sci & veterinary Med Cluj-napoca, 2020) Akdag, Seref; Tuncer, Burcu
Eremurus spectabilis is a perennial herbaceous species that grows in the wild. Commonly consumed as a vegetable, it is also classified among medicinal plants and popular ornamental geophytes. Germination is not achieved under normal conditions because of seed dormancy. In this study, various combined treatments were experimented with to improve the germination and emergence performance of E. spectabilis seeds. The seeds were immersed in solutions of potassium nitrate (KNO3: 5 mM for 24 and 48 hours), calcium chloride (CaCl2: 5 mM for 24 and 48 hours) and subjected to tip cutting. Pre-treated seeds were then subjected to moist-cold stratification periods treatments for different durations (30, 50, 80 and 100 days). In the 5 mM KNO3 treatments, 48-hour KNO3 and 100 days of stratification treatments had better germination (11.1%) and emergence values (9.7%). In the 5 mM CaCl2 treatments, 24-hour CaCl2 yielded better germination (10.2%) and emergence (8.4%) values. Seed tip-cutting treatments were the most successful among all the treatments for the germination and emergence parameters.
Effects of Moist-Cold Stratification and Gibberellic Acid Applications on Breaking Seed Dormancy in Foxtail Lily (Eremurus Spectabilis M.bieb.)
(2020) Akdag, Seref; Tuncer, Burcu
Eremurus spectabilis is a wild edible perennial herbaceous geophyte species with medicinal and popularornamental uses. In this study, gibberellic acid (GA3) with moist-cold stratification treatments were applied to break thedormancy of E. spectabilis seeds. For this purpose, seeds were soaked in gibberellic acid solutions (GA3: 500 and 750 ppmfor 24 and 48 hours). Pretreated seeds were then subjected to moist-cold stratification treatments for different durations (30,50, 80 and 100 days) at 4 ºC. In 24-hour GA3 treatments, the germination and emergence ratios (21.04%) were obtainedfrom the 500 ppm GA3 dose and 100 days of stratification treatments. In 48-hour GA3 treatments, the GA3 doses did nothave any significant effect on germination and emergence ratios; the greatest germination ratios were obtained from the 500ppm (20.36%) and 750 ppm (20.00%) doses and 100 days of stratification treatments, and the best emergence ratios wereobtained from the 500 ppm GA3 dose and 100 days of stratification treatments (18.51%). It is considered that it is beneficialto try the combinations of moist-cold applications with different treatments in future studies.
Establishment in Protocol for in Vitro Micropropagation of Ciris (eremurus Spectabilis M.bieb)
(2021) Sıcakyüz, Saadet; Tuncer, Burcu
Doku kültürü, Eremurus spectabilis M.Bieb, Liliaceae, Soğancık rejenerasyonu.
In Vitro Germination and Bulblet and Shoot Propagation for Wild Edible Eremurus Spectabilis M.bieb
(Univ Agr Sci & veterinary Med Cluj-napoca, 2020) Tuncer, Burcu
Eremurus spectabilis M.Bieb is consumed as a vegetable because of its nutritious characteristics. The plants are also used for medicinal purposes, in the cut flower industry as an ornamental geophyte, and in industry as a natural adhesive. The aim of the present study was to improve the in vitro propagation protocol for germination and bulblet/shoot formation ofE. spectabilis. For this purpose, E. spectabilis seeds were in vitro germinated in four different nutrient media: Murashige and Skoog (MS), Gamborg (B5), White (WH), and Shenk and Hildebrandt (SH). To stimulate bulblet and/or shoot regeneration, hypocotyls of 35-40-day-old in vitro-germinated plantlets were cut into 0.5-1.0 cm pieces, and the resultant explants were cultured in MS media containing 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5, 1.0, 2.0, and 4.0 mg L-1) + kinetin (0.5 mg L-1), thidiazuron (TDZ) (0.5, 1.0, 2.0, and 4.0 mg L-1) + 1-naphthylacetic acid (NAA) (0, 0.1, 0.5, and 1.0 mg L-1), and 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 4.0 mg L-1) + 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 0.1, 0.5, and 1.0 mg L-1). The best outcomes for germination ratio (57.5%) were obtained from the B5 medium. In the third set of in vitro propagation experiments, 100% bulblet formation was achieved in TDZ (0.5 mg L-1) and NAA (0.5 and 0.1 mg L-1) combinations of MS media, and this was followed by 0.5 mg LA BAP-containing medium (81.3%). Shoot formation ratios with the same media combinations varied from 60-70%, and the number of shoots per explant varied from 1.4-2.4 shoots. Further in vitro propagation research is planned with larger bulb sizes to develop a protocol for rooting bulblets and/or shoots.
Optimizing Culture Media for in Vitro Microbulb Production of Medicinal Wild Plant: Eremurus Spectabilis M. Bieb
(inst investigaciones Agropecuarias - inia, 2022) Sicakyuez, Saadet; Tuncer, Burcu
Eremurus spectabilis M. Bieb. is an important wild plant species consumed as a vegetable. The plant is also used for medicinal purposes, in the cut flower industry as ornamental geophytes. In this study, it was aimed to ensure germination, bulblet regeneration, bulblet size enlargement in E. spectabilis under in vitro conditions. Gamborg (B5) and White (WH) media were used for in vitro germination of seed tip-cutting and total of 24 Murashige and Skoog (MS) combinations supplemented with thidiazuron (TDZ) (0.5, 1.0, 2.0, and 4.0 mg L-1) + naphthalene acetic acid (NAA) (0, 0.1, and 0.5 mg L-1) and 6-benzyl amino purine (BAP) (0.5, 1.0, 2.0, and 4.0 mg L-1) + NAA (0, 0.1, and 0.5 mg L-1) were used for bulblet regeneration experiments. For bulblet size enlargement, two trials were set up with an interval of 30-35 d. The highest germination ratio (61.5%) was obtained from B5 medium (p < 0.01). In in vitro bulblet regeneration trials, MS media supplemented with 0.5 mg L-1 TDZ with 0.1 (65.8%) and 0.5 mg L-1 NAA (65.3%) and the combination of 1.0 mg L(-1 )TDZ with 0.5 mg L-1 NAA (65.1%) yielded more successful in terms of bulblet ratio (p < 0.01). For bulblet size enlargement, as the average of both trial sets, 1.0 mg L-1 TDZ+0.1 mg L-1 NAA combination was more successful in terms of bulblet ratio (79.05%) and bulblet diameter (2.05 cm). The most successful combinations in terms of average number of bulblets were 2.0 mg L(-1 )TDZ+0.1 mg L(-1 )NAA (32.25 bulblets) and 1.0 mg L-1 TDZ+0.1 mg L-1 NAA (30.6 bulblets), respectively.
Phosphorus and Humic Acid Application Alleviate Salinity Stress of Pepper Seedling
(Academic Journals, 2010) Cimrin, K. Mesut; Turkmen, Onder; Turan, Metin; Tuncer, Burcu
Humic acid is a commercial product that contains many elements which improve the soil fertility and increase the availability of nutrient elements. It consequently affects plant growth and yield and ameliorates the deleterious effects of salt stress. The objective of the study was to determine the effect of humic acids and phosphorus on growth and nutrient content of pepper seedlings (cv. Demre) grown under moderate salt stress in growth chamber conditions. Applications of different levels of phosphorus [0 (P(0)), 50 (P(1)), 100 (P(2)) and 150 (P(3)) mg kg(-1)] and humic acid [0 (HA(0)), 750 (HA(1)) and 1500 (HA(2)) mg kg(-1)] to growing media containing moderate salt dose (8 mM NaCl treatment) were studied. The study was replicated four times with 20 plants in each replicate. Humic acid (HA) and phosphorus applications increased the growth and growth parameter of plants. In company, effects of HA and P application was more effective on growth and growth parameter than each separate effect. The optimum total yield was obtained from 69 mg kg(-1) P application with HA2 doses according to regression analysis. Humic acid application significantly increased N, P, K, Ca, Mg, S, Mn and Cu contents of shoot of pepper seedling. Also, N, P, K, Ca, S, Fe, Mn, Zn and Cu contents of root were increased with humic acid application. Na contents of both shoot and root of pepper decreased with increased humic acid doses. It can be concluded that high humic acid doses has positive effects on salt tolerance based on the plant growth parameters and nutrient contents. The present study suggests that HA treatments can ameliorate the deleterious effects of salt stress on pepper plants and HA could offer an economical and simple application to reduce problems of pepper production in moderately saline soil.
Reaction of Hybrid Ornamental Dales To in Vitro Salt Stress During the Germination and Emergence Periods
(Parlar Scientific Publications (p S P), 2017) Tuncer, Burcu
This study aimed to determine the reactions of five F-1 hybrid ornamental kale cultivars (Red Pigeon, Victoria Pigeon, Red Chidori, White Kamome, and Pink Kamome) to in vitro salt stress during germination and emergence. Sterilized seeds were cultured on Murashige and Skoog (MS) media containing four different NaCl doses (0, 3, 7, and 12 dS/m). According to the obtained results, the germination rate, speed of germination, average germination time, germination index, and sensitivity index values were 42.72-97.09%, 0.76-3.43, 3.08-6.80 days, 6.60-41.28, and 0.81-1.51, respectively. Emergence rate, speed of emergence, the average emergence time, emergence index, hypocotyl length and radicle length values were detected as 11.99-93.78%, 0.16-1.63, 4.97-8.69 days, 1.45-18.75, 0.39-3.27 cm and 0.68-3.43 cm, respectively. The F-1 Red Pigeon and F-1 Victoria Pigeon were the most salt stress resistant cultivars, while the F-1 White Kamome cultivars were the most susceptible to salt stress. Increasing the NaCl dose had a statistically significant negative effect on both the germination and emergence properties of the cultivars.
Research on Overcoming the Dormancy Problem of Molekhia (Corchorus Olitorius L.) Seeds
(2017) Ummuhan, Fatima; Tuncer, Burcu
Bu araştırmada; lif bitkisi ve tıbbi bitki olarak kullanımının yanı sıra, sebze olarak da tüketim değeri olan molehiya (Corchorus olitorius L.) bitkisinin tohumlarında, dormansiyi kırmak için farklı tohum uygulamalarının çimlenme ve çıkış üzerine etkisi amaçlanmıştır. Bu amaçla sterilizasyonu yapılan tohumlara; kaynayan su (95 ºC; 10 ve 30 saniye, 1, 5 ve 10 dakika), sıcak su (80 ºC; 5, 10, 15 ve 20 dakika), sülfürik asit (% 98'lik; 5, 10, 15, 20, 25 ve 30 dakika) ve ön soğutma uygulamaları (4ºC; 2, 3 ve 4 gün) yapılmıştır. Denemeler; laboratuvar ortamında, 4 tekerrürlü ve her tekerrürde 50 tohum olacak şekilde tesadüf parselleri deneme desenine göre yürütülmüştür. Elde edilen sonuçlara göre; çimlenme oranı % 0.0097.00, ortalama çimlenme süresi 0.00-3.98 gün, çimlenme indeksi 0.00-46.89, çıkış oranı % 0.00-82.00, ortalama çıkış süresi 0.00-7.52 gün, çıkış indeksi 0.00-18.70, hipokotil boyu 0.00-1.79 cm ve kökçük boyu ise 0.00-4.28 cm arasında değişim göstermiştir. Çimlenme ve çıkış parametreleri bakımından en başarılı uygulamalar; ön soğutma, sülfürik asit (5 ve 10 dakika) ve kaynayan su (95 ºC, 10 saniye) uygulamaları olmuştur. Çalışma sonucunda, Corchorus olitorius L. tohumlarında dormansiyi ortadan kaldırmada, ekimden önce tohumların 2 gün süreyle nemli ortamda soğukta (4 ºC) bekletilmesinin pratikte de rahatlıkla uygulanabileceği sonucuna ulaşılmıştır
Yabani Ravent (Rheum Ribes L.)’in Doku Kültürü ile Çoğaltım Olanakları Üzerine Araştırma
(2017) Tuncer, Burcu; Günsan, Büşra
Rheum ribes L. tıbbi öneme sahip çok yıllık yabani bir bitki türüdür. Bu çalışmada, in vitro koşullarda büyütülmüş 7 günlük fidelerden alınan farklı eksplant tipleri (hipokotil ve kotiledon), Indol-3-butirik asit (IBA) (0.0 ve 1.0 mg L-1) ve 6Benzilaminopurin (BAP) (0.0, 1.0 ve 2.0 mg L-1) kombinasyonları ilave edilmiş MS (Murashige and Skoog) besin ortamında kültüre alınmıştır. Araştırma sonucunda sürgün ve kallus oluşumu yönünden hipokotil eksplantlarının kotiledon eksplantlarından daha başarılı olduğu belirlenmiştir. Hipokotil eksplantlarında, eksplant başına en yüksek sürgün sayısı (4.0 adet eksplant-1) 2mg L-1 BAP ve 1 mg L-1 IBA ilavesi yapılmış MS ortamından (MS6) elde edilirken, kallus oluşum oranı açısından ise MS4 (MS + 1 mg L-1 BAP + 1 mg L-1 IBA) (% 88.8) ve MS6 (MS + 2 mg L-1 BAP + 1 mg L-1 IBA) (% 83.3) ortamlarının daha başarılı olduğu tespit edilmiştir