Browsing by Author "Tuncer, M.C."

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Investigation of the Neuro-Regenerative Effects of Bioresonance and Magnetotherapy in Sciatic Nerve Damage-Induced Rats
(Universidad de la Frontera, 2023) Tas, A.; Nalci, K.A.; Sincan, S.; Özdemir, H.; Üstün, R.; Aras, I.; Tuncer, M.C.
Peripheral nerve damage is a significant clinical problem that can lead to severe complications in patients. Regarding the regeneration of peripheral nerves, it is crucial to use experimental animals' nerves and use different evaluation methods. Epineural or perineural suturing is the gold standard in treating sciatic nerve injury, but nerve repair is often unsuccessful. This study aimed to investigate the neuroregenerative effects of magnetotherapy and bioresonance in experimental animals with sciatic nerve damage. In this study, 24 female Wistar rats were divided into 7 groups (n=6) as follows: Group 1 (Control), Group 2 (Axonotmesis control), Group 3 (Anastomosis control), Group 4 (Axonotmesis + magnetotherapy), Group 5 (Anastomosis + magnetotherapy), Group 6 (Axonotmesis + bioresonance), Group 7 (Anastomosis + bioresonance). Magnetotherapy and bioresonance treatments were applied for 12 weeks. Behavioural tests and EMG tests were performed at the end of the 12th week. Then the rats were sacrificed, and a histopathological evaluation was made. The statistical significance level was taken as 5% in the calculations, and the SPSS (IBM SPSS for Windows, ver.21) statistical package program was used for the calculations. Statistically significant results were obtained in animal behaviour tests, EMG, and pathology groups treated with magnetotherapy. There was no statistically significant difference in the groups treated with bioresonance treatment compared to the control groups. Muscle activity and nerve repair occurred in experimental animals with acute peripheral nerve damage due to 12 weeks of magnetotherapy, and further studies should support these results. © 2023, Universidad de la Frontera. All rights reserved.
Silymarin Protected the Cerebral Tissue from Endoplasmic Reticulum Stress
(Via Medica, 2025) Karaaslanlı, A.; Tuncer, M.C.; Aşır, F.; Korak, T.
Background: Our aim is to explore silymarin’s protective effects against endoplasmic reticulum (ER) stress via protein kinase R-like endoplasmic reticulum kinase (PERK) modulation, and elucidate potential enriched pathways through in silico analysis of silymarin-associated PERK protein interactors in cerebral ischaemia-reperfusion (IR) injury. Materials and methods: 30 rats were categorized into three groups: sham, IR, and IR + silymarin. Cerebral IR damage was not induced. Only the MCA was identified and clamped without further intervention. The sham group received only physiological serum intravenously. In the IR group, rats were exposed to 2 hours ischaemia and then 3 hours of reperfusion. In the IR + silymarin group the rats received 1 μg/kg silymarin intravenously (i.v.) before induction of cerebral IR. Cerebral tissues were processed for histological tissue preparation. Haematoxylineosin and PERK immunostaining were applied. In Cytoscape software, we imported and integrated the silymarin and PERK protein-protein interaction networks generated from the STITCH and STRING databases, respectively. Subsequently, Reactome pathway annotation was performed for this intersected network. Results: In the sham group, neurons were large and round with oval nuclei, and no histopathological changes were observed. In the IR group, neurons and neuroglial cells showed degeneration with pyknotic nuclei, apoptotic bodies, dilated and congested cerebral capillaries, and numerous vacuoles. After silymarin treatment, the IR + silymarin group showed a restoration of normal histology, with more regular neural and neuroglial cells and decreased vessel dilation and congestion. PERK immunoexpression was mainly negative in the sham group, increased in the IR group, and decreased again in the IR + silymarin group. Upon intersecting the interactors of silymarin and PERK, 17 common proteins were identified. Reactome pathway analysis revealed potential impacts of these proteins on key pathways including immune and cytokine signaling, apoptosis, estrogen signaling, and extracellular matrix degradation. Conclusions: Silymarin’s targeting of PERK offers a promising approach to alleviate ER stress and potentially modulate multiple critical pathways in cerebral ischaemia reperfusion, serving as a comprehensive therapeutic strategy for managing cerebral IR injury. (Folia Morphol 2025; 84, 3: 534–543). © 2025 Elsevier B.V., All rights reserved.