Browsing by Author "Turgut, A.O."

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Comparison of Serum Biochemical Parameters in Ketotic and Healthy Siirt Colored Mohair Goats During Early Lactation to Identify Potential Biomarkers of Lactation Ketosis; Comparación De Parámetros Bioquímicos Séricos En Cabras De Mohair De Color Siirt Con Cetosis Y Sanas Durante La Lactancia Temprana Para Identificar Posibles Biomarcadores De Cetosis De Lactancia
(Universidad del Zulia, Facultad de Ciencias Veterinarias, 2025) Irmak, M.; Turgut, A.O.; Eroğlu, M.; İRak, K.; Tufan, T.; Koca, D.; Keskin, I.H.
This study aimed to compare serum biochemical parameters between ketotic and healthy Siirt colored mohair goats during early lactation, to diagnose subclinical lactation ketosis and identify potential biomarkers. A total of 77 female goats, aged 2 to 5 years and within 30 days postpartum, were evaluated under similar management conditions. The animals were classified into two groups based on serum β–hydroxybutyric acid levels: subclinical lactational ketosis (n = 37) and health controls (n = 40). β–hydroxybutyric acid levels were significantly elevated in the ketosis group (0.891 ± 0.0141 mmol·L-1) compared to the control group (0.595 ± 0.0159 mmol·L-1, P<0.001), confirming its diagnostic value. Other serum parameters did not show significant differences between the groups (P>0.05). On the other hand, effect size analysis revealed that glucose level decreased in does with subclinical lactational ketosis while cholesterol increased (large effect size). The study concluded that most liver and metabolic indicators remained within normal ranges, while glucose and cholesterol levels changed due to subclinical lactational ketosis. These data indicate that feeding based on pasture under extensive conditions may be insufficient to meet the energy requirements of does in lactation and emphasize the importance of early biochemical screening during lactation for effective management of metabolic disorders during early lactation stage in goats. Therefore, it is recommended the further studies to expand the knowledge of the effect of lactational ketosis on metabolic changes in goats. © 2025 Elsevier B.V., All rights reserved.
The Elecsys AMH Assay Is a Suitable Method to Detect Gonadal Tissue in Male Alpacas and Llamas
(John Wiley and Sons Inc, 2025) Sendag, S.; Wagner, H.; Turgut, A.O.; Koca, D.; Schuler, G.; Wehrend, A.
Objective: Anti-Müllerian hormone (AMH) has become an important hormonal parameter for the detection of gonadal tissue and for the diagnosis of gonadal functions and pathologies. To our knowledge, there is currently no homologous test for AMH measurements in South American camelids (SACs). Therefore, the objective of the present study was to determine serum AMH concentrations in postpubertal male alpacas and, for the first time, in llamas, using the Elecsys AMH assay kit that has not previously been tested in these species. To obtain indications of the specificity of this method in SAC, measurements were carried out in male gelding in which concentrations below the detection limit were to be expected. Methods: In this context, 37 blood samples collected by jugular venipuncture from 21 alpacas and 16 llamas were used. The obtained blood was centrifuged at 3000 g for 20 min, and the serum was stored in Eppendorf tubes at −20°C until AMH concentrations were measurement. The measurement of AMH levels was conducted in a commercial diagnostic laboratory (Laboklin, Bad Kissingen, Germany) using the electrochemiluminescence immunoassay kit Elecsys AMH run on the fully automated Cobas e 601 analyser (Roche Diagnostics Deutschland GmbH, Mannheim). The AMH test had a minimum detection limit of 0.01 ng/mL and a maximum detection limit of 23 ng/mL. The intra-assay coefficient of variation is between 2.7% and 3.3%. Results: Blood serum AMH levels ranged between 4.10 and 22 ng/mL (median: 9.80 ng/mL) and 1.79 and 10.05 ng/mL (median: 4.00) in intact alpacas (age: 6.30 ± 2.71 years; n = 10) and llamas (age: 5.50 ± 4.34; n = 8), respectively, and were significantly different between samples obtained from the two species (p < 0.05). Correlation analyses regarding an age dependence of AMH concentrations yielded negative correlation coefficients for both species but non-significant p values (alpaca: r = −0.165, p = 0.649; llama: r = −0.547, p = 0.160). In alpaca (n = 11) and llama geldings (n = 8), blood serum AMH levels were below 0.01 ng/mL (p < 0.001). These results prove that the antibodies used in the Elecsys AMH assay significantly and specifically cross-react with SAC AMH. Conclusions: In gelding llamas and alpacas, AMH concentrations were below the limit of detection (<0.01 ng/mL), which was significantly lower compared to intact animals (p < 0.001). The Elecsys AMH assay is therefore considered a suitable method for detecting gonadal tissue in SAC. © 2025 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd.