Browsing by Author "Turgut, Ali Osman"

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Anti-Mullerian Hormone as a Promising Novel Biomarker for Litter Size in Romanov Sheep
(Wiley, 2024) Turgut, Ali Osman; Koca, Davut
Anti-Mullerian hormone (AMH) is a hormone produced by growing preantral and antral follicles of the ovary. AMH is accepted as an important biomarker for fertility and superovulation parameters in livestock species. This study aimed to evaluate changes in serum AMH level in the oestrous cycle, repeatability of AMH, the effect of age on serum AMH level and the effects of AMH on litter size in Romanov sheep. In the study, a total of 36 Romanov sheep were used as animal material. First blood samples (0th day) were collected from 36 ewes to evaluate AMH and progesterone levels. Second blood samples were collected randomly from 20 ewes 9 days after first sampling to compare AMH levels at two different periods of the oestrous cycle in Romanov ewes. The ewes were categorized into three groups as low, medium and high AMH based on their first AMH levels. Results indicated that serum AMH level did not change during the oestrous and dioestrous phases of the oestrous cycle and two random time points of the oestrous cycle (p > .05). Pearson correlation analysis revealed that there is a high (r = .95) and significant (p < .001) correlation between AMH levels at the 0th (AMH-1) and 9th (AMH-2) days. The effect of AMH level on litter size was found to be significant. Litter size was significantly higher in the high AMH group than in the low AMH group (p < .05). In addition, the age of ewes did not affect serum AMH levels (p > .05). ROC analysis indicates that AMH cut-off value >320 pg/mL with 70% sensitivity and 100% specificity can be used for litter size in Romanov ewes. In conclusion, AMH is highly repeatable and its serum AMH level did not change during the oestrous cycle in Romanov sheep. In addition, AMH affects litter size and can be reliably used as a marker for litter size in Romanov sheep.
Anti-Müllerian Hormone: a Novel Biomarker for Detecting Bovine Freemartinism
(Wiley, 2024) Koca, Davut; Nak, Yavuz; Sendag, Sait; Nak, Deniz; Turgut, Ali Osman; Avcilar, Talha; Wehrend, Axel
The anti-M & uuml;llerian hormone (AMH) indicates ovarian reserve in cattle, maintaining a consistent trajectory post-puberty. In heterosexual pregnancies, the development of the M & uuml;llerian duct in female foetuses is inhibited, resulting in an anticipated minimal or absent ovarian reserve capacity. This investigation aimed to compare AMH levels in healthy Holstein heifers that had reached puberty with those of freemartin animals of the same breed and age. The study incorporated Holstein heifers reaching puberty between 11 and 15 months of age in Group 1 (G1, n = 20) and freemartin animals in Group 2 (G2, n = 19, 16). AMH measurements (AMH-1/AMH-2) were recorded at 12-day intervals for the study participants. Notably, AMH levels in three freemartin animals could not be detected, prompting statistical analysis based on measurements from the remaining 16 freemartin animals in G2. A statistically significant correlation was observed between two separate measurements in G1 and G2 (p < .001). Furthermore, AMH-1 and AMH-2 levels were statistically higher in G1 than in G2 (p < .001). In G1, AMH-1 levels ranged from 227 to 677 pg/mL, with an average of 367.3 +/- 25.5 pg/mL, and AMH-2 levels ranged from 234 to 645 pg/mL, with an average of 380.8 +/- 24.4 pg/mL. Conversely, in G2, AMH-1 levels ranged from 10 to 72 pg/mL, with an average of 26.8 +/- 4.44 pg/mL, and AMH-2 levels ranged from 12 to 68 pg/mL, with an average of 28.75 +/- 4.18 pg/mL. The mean AMH levels in G1 were approximately 14 times higher than in G2 (p < .001). Consequently, ROC analysis utilizing AMH-1 and AMH-2 data established cut-off values of <= 72 and <= 68 pg/mL respectively for distinguishing freemartin animals. In conclusion, AMH could be used as a reliable biomarker for identifying Holstein freemartin animals.
Comparison of Blood Βhba Measurement Devices for Diagnosis of Subclinical Pregnancy Toxaemia in Sheep: a Field Study
(Wiley, 2024) Turgut, Ali Osman; Koca, Davut; Unver, Ali
Pregnancy toxaemia is a serious disease that occurs during the last trimester of pregnancy in sheep. Yet, in most cases, the disease may have a subclinical course. This study was aimed at comparing blood beta HBA measurement devices for diagnosis of subclinical pregnancy toxaemia in late pregnant sheep. In the study, a total of 50 blood samples were collected from Romanov (n = 30) and cross-bred Hamdani (n = 20) sheep. Blood beta HBA levels were measured using TaiDoc TD-4235 and CentriVet beta HBA hand-held meter. Randox beta HBA (Ranbut) assay was used as a reference laboratory method to compare hand-held meters. beta HBA value of 0.8 mmol/L was set as the cut-off value for diagnosis of subclinical pregnancy toxaemia. Statistical analyses were carried out using Minitab 21 and Jamovi software. In the study, the correlation of Randox-TaiDoc TD-4235 and Randox-CentriVet was .822 (p < .001) and .728 (p < .001), respectively. Based on the Ranbut assay, nine ewes out of 50 were diagnosed with subclinical pregnancy toxaemia. Specificity (detection of healthy ewes) and sensitivity (detection of ewes with subclinical pregnancy toxaemia) for TaiDoc TD-4235 and CentriVet hand-held meters were 100%, 77.8%, and 100%, 66.7%, respectively. In the receiver operating characteristic (ROC) analysis, areas under the ROC curve (AUC) were 0.976 and 0.920 for TaiDoc and CentriVet, respectively. Bland-Altman analysis revealed a bias of 0.092 mmol/L for TaiDoc and a bias of 0.132 mmol/L for CentriVet. TaiDoc hand-held meter shows a better correlation with the Randox Ranbut assay and greater sensitivity compared to the CentriVet hand-held meter. In conclusion, both TaiDoc and CentriVet hand-held meters can be securely used in the diagnosis of subclinical pregnancy toxaemia in sheep. For these reasons, subclinical pregnancy toxaemia and these devices will be evaluated within the scope of herd management programme in the sheep industry. It should also be taken into account that these conditions will affect the future fertility of the mother and offspring.
Comprehensive Evaluation of Changes in Placentomes in the Second and Third Trimesters of Pregnancy in Cross-Bred Hamdani Sheep
(Wiley, 2025) Kandil, Banu; Turgut, Ali Osman; Koca, Davut; Isbilir, Fatma; Atli, Muhammed Zahid; Guzel, Baris Can
Background: A proper placentation is required for establishment and continuity of pregnancy. In sheep, placentomes are unique structures that enable nutrition and gas exchange between the mother and the foetus. Although placentomes are dynamic formations, there is limited knowledge of changes in placentomes during pregnancy. Objective: This study aimed to identify changes in sheep placentomes in the second and third trimesters of pregnancy using both macroscopic and microscopic methods. Methods: This study investigated 14 healthy cross-breed Hamdani sheep placentomes, comprising seven second and seven third trimesters of pregnancy. The histomorphometric analysis included measurements of capillary number and area in cotyledonary and caruncular regions, while morphometric assessments encompassed placentome dimensions such as number, length, width, and depth. Results: Placentomes were oval and circular in shape in the second and third trimesters. In the second trimester, they were observed as concave structures with thick edges, whereas in the third trimester, they were determined as thin-edged structures with a slight depression in the centre. In the third trimester, foetal and maternal tissues became more intertwined with increased branching of foetal villi and maternal crypts. Placental hematomas and erythrocytes in the cytoplasm of trophoblast cells were more prominent in the third trimester. Statistical analysis revealed no difference in placentome number between the second and third trimesters. However, the dimensions (length, width, and depth) of placentomes were greater in the third trimester compared to the second trimester (p < 0.001). Additionally, while there was no difference in the number of cotyledonary versus caruncular capillaries in the second trimester, cotyledonary capillaries outnumbered caruncular capillaries in the third trimester (p < 0.001). Furthermore, both cotyledonary and caruncular capillary areas increased in the third trimester compared to the second trimester, with the caruncular capillary area being consistently higher than the cotyledonary capillary area in both trimesters (p < 0.05). Conclusion: This study underscores the substantial structural and physiological transformations of placentomes in the second and third trimesters of pregnancy in sheep. These adaptations facilitate efficient flow exchange between the foetus and mother, highlighting the dynamic nature of placental development during late gestation.
The Effects of Case/Control Ratio and Sample Size on Genome-Wide Association Studies: a Simulation Study
(Wiley, 2024) Turgut, Ali Osman; Koca, Davut
BackgroundGenome-wide association studies (GWAS) is a useful tool for the detection of disease or quantitative trait-related genetic variations in the veterinary field. For a binary trait, a case/control experiment is designed in GWAS. However, there is limited information on the optimal case/control and sample size in GWAS.ObjectivesIn this study, it was aimed to detect the effects of case/control ratio and sample size for GWAS using computer simulation under certain assumptions.MethodUsing the PLINK software, we simulated three different disease scenarios. In scenario 1, we simulated 10 different case/control ratios with increasing ratio of cases to controls. In scenario 2, we did versa of scenario 1 with the increasing ratio of controls to cases. In scenarios 1 and 2, sample size gradually was increased with the change case/control ratios. In scenario 3, the total sample size was fixed to 2000 to see real effects of case/control ratio on the number of disease-related single nucleotide polymorphisms (SNPs).ResultsThe results showed that the number of disease-related SNPs were the highest when the case/control ratio is close to 1:1 in scenarios 1 and 2 and did not change with an increase in sample size. Similarly, the number of disease-related SNPs was the highest in case/control ratios 1:1 in scenario 3. However, unbalanced case/control ratio caused the detection of lower number of disease-related SNPs in scenario 3. The estimated average power of SNPs was highest when case/control ratio is 1:1 in all scenarios.ConclusionsAll findings led to the conclusion that an increase in sample size may enhance the statistical power of GWAS when the number of cases is small. In addition, case/control ratio 1:1 may be the optimal ratio for GWAS. These findings may be valuable not only for veterinary field but also for human clinical experiments. An increase in sample size may enhance the statistical power of GWAS when the number of cases is small. Case/control ratio 1:1 may be the optimal ratio for GWAS. These findings may be valuable not only for veterinary field but also for human clinical experiments. image
Elecsys® Amh Assay: Determination of Anti-Müllerian Hormone Levels and Evaluation of the Relationship Between Superovulation Response in Holstein Dairy Cows
(Wiley, 2024) Koca, Davut; Aktar, Ahmet; Turgut, Ali Osman; Sagirkaya, Hakan; Alcay, Selim
Background: Anti-Mullerian Hormone (AMH) serves as a crucial parameter in assessing the reproductive herd life and ovarian reserve in cattle. Consequently, extensive research is conducted on AMH levels. Various measurement methods can be employed to determine AMH levels. However, to our knowledge, no study has been conducted on Holstein donors using the Elecsys (R) AMH kit. Objective: This study was designed to determine AMH levels in donors utilising the Elecsys (R) AMH kit and to evaluate the relationship between superovulation response parameters and AMH levels. Methods In this study, we measured the serum AMH levels of 36 cows using the Elecsys (R) AMH automated assay before the superovulation protocol (1st sample) and FSH injections (2nd sample). The cows were categorised into three groups based on their AMH levels: low, medium, and high AMH. Results: Positive correlations were identified between AMH and parameters associated with superovulation response. The high AMH level group exhibited significantly greater numbers of corpus luteum, total embryos, transferable embryos, and grade 1 embryos compared to the medium and low AMH groups (p < 0.05) There was no significant difference between AMH levels before the superovulation protocol and FSH injections(p > 0.05). Body condition score and parity did not significantly affect AMH levels in cows (p > 0.05). Also, AMH cut-off values for the number of corpus luteum, total embryo, and transferable embryos were detected as 234, 227, and 210 pg/mL, respectively. Conclusion: These findings demonstrate that a high serum AMH level has a positive influence on the superovulation response. AMH can be used as a reliable marker for the selection of donors in Holstein cows.
Melatonin Receptor 1a (Mtnr1a) Gene Polymorphism in Cross-Bred Hamdani Sheep: a Preliminary Study
(2024) Turgut, Ali Osman; Koca, Davut
Melatonin plays a key role in regulating several vital physiological processes, including the maturation and functioning of the reproductive system, pubertal development, seasonal reproduction, and adaptation. The activation of the melatonin hormone is facilitated by melatonin receptors. This study aimed to investigate melatonin receptor 1A (MTNR1A) gene polymorphism in cross-bred Hamdani sheep. A total of 102 cross-bred Hamdani ewes were used as animal material. DNA was extracted from whole blood. The 824 bp PCR products from the exon II region of the MTNR1A gene underwent analysis for restriction fragment length polymorphism (RFLP) using the RsaI restriction enzyme. In the study, RR, Rr, and rr genotypes were detected for MTNR1A gene in cross-bred Hamdani sheep. Genotype frequencies for RR, Rr, rr were 0.69, 0.27, and 0.04, respectively. Allele frequencies were detected as 0.82 and 0.18 for R and r alleles, respectively. In conclusion, more comprehensive research investigating association between MTNR1A genotypes and reproductive traits should be carried out in cross-bred Hamdani sheep.
Milk Composition Traits of Hamdani Crossbreed Sheep Raised Under Extensive Management
(2023) Turgut, Ali Osman; Gülendağ, Erman; Koca, Davut; Üner, Sefa
This study aimed to reveal the milk composition traits of Hamdani crossbred sheep (Hcs) raised under extensive conditions. A total of 96 sheep milk samples were collected during the early lactation stage. Selected ewes did not have clinical mastitis or subclinical mastitis. Milk composition analyses were performed using milk autoanalyzer. Mean values for milk fat (%), solid non-fat (SNF) (%), protein (%), lactose (%), salt (%) and pH, conductivity (mS/cm), freezing point (⸰C) and density (kg/m3) in Hcs were determined as 7.49±0.15, 8.69±0.08, 4.13±0.04, 3.89±0.04, 0.62±0.01, 6.93±0.04, 4.05±0.07, -0.5±0.01, and 1.027.84±0.38 respectively. In correlation analysis, negative and significant correlations were detected between milk fat and protein (r=- 0.368, p<0.001), milk fat and SNF (r=-0375, p<0.001) milk fat and lactose (r=-0.380, p<0.001). On the other hand, positive and significant correlations were detected between SNF and milk protein (r=0.999, p<0.001), SNF and salt (r=0.963, p<0.001), and SNF and lactose (r=0.976, p<0.001). As a result, the milk fat percentage of Hcs was higher compared to other native sheep breeds of Turkey. In addition, phenotypic variation for milk fat percentage was found to be high among individuals in the same flock. These findings may contribute to new studies to improve milk composition traits in Hamdani crossbred sheep.
Molecular Survey of the Toxoplasma Gondii and Neospora Caninum in Brain Tissue of Aborted Fetuses of Morkaraman Sheep in Muş, Türkiye
(2023) Turgut, Ali Osman; Koca, Davut; Celık, Burcak Aslan; Ayan, Özge Oktay; Çelik, Özgür Yaşar; Ayan, Adnan; Kılınç, Özlem Orunç
Toxoplasma gondii and Neospora caninum are obligate intracellular protozoan parasites that can affect different warm-blooded species worldwide. In this study, it was aimed to detect T. gondii and N. caninum using PCR method in brain tissues of aborted sheep fetuses. Brain specimens were collected from 50 Morkaraman sheep fetuses that had undergone abortion at various stages of pregnancy, within the lambing seasons of 2023 in Muş. Approximately 1 cm³ of brain tissue from the right cerebral hemisphere was excised and subsequently frozen at -20°C for DNA extraction. DNA extraction and PCR amplification were then performed. As a result of this study, 11 (22%) of 50 brain tissues were positive. All brain samples examined in this study were negative for Neospora caninum. Based on the results of this study, it is possible to say that T. gondii is an important abortion agent in sheep in this region. Although N. caninum was not detected in this study, larger scale studies are recommended. Moreover, this study provides important information to breeders and veterinarians in the evaluation and management of abortion in the field.
Molecular Survey of Toxoplasma Gondii Infection in Aborted Fetuses of Sheep in the Iğdır Province of Türkiye
(2023) Koca, Davut; Kılınç, Özlem Orunç; Ayan, Adnan; Oğuz, Fatma Ertaş; Turgut, Ali Osman; Ayan, Özge Oktay
Toxoplasma gondii, an obligatory intracellular protozoan parasite, can infect a wide range of warm- blooded animals, including livestock species. T. gondii is a zoonotic protozoan parasite that affects both humans and other warm-blooded animals. The aim of this study was to detect T. gondii by using PCR in the brain tissues of 60 aborted sheep fetuses from the Iğdır Province in Türkiye. For this purpose, 60 brain tissue samples of sheep were collected within the lambing seasons of 2023 in Iğdır, Türkiye. The DNA extraction was performed using the PureLink™ Genomic DNA Mini Kit from brain samples. The PCR was performed with the appropriate primers from the obtained DNA samples. T. gondii was found in the brain (16.6%) samples of aborted sheep fetuses. According to the present study, T. gondii infection can be one of the causes of fetus abortion of sheep in Iğdır province, Türkiye. This result emphasizes the need for vigilance and preventive measures in managing this potential public and animal health concerns.
The Negative Effects of Subclinical Pregnancy Toxaemia on Fetal Skeletal Muscle Development and Evaluation of the Protective Effects of Dietary L-Carnitine Supplementation in Sheep
(Wiley, 2025) Turgut, Ali Osman; Kandil, Banu; Bilen, Ebru Karakaya; Koca, Davut; Unver, Ali; Isbilir, Fatma; Wehrend, Axel
This study evaluated the effects of subclinical pregnancy toxaemia (SPT) on fetal skeletal muscle development and assessed the potential protective role of L-carnitine supplementation during gestation. A total of 18 crossbred Hamdani ewes underwent oestrous synchronisation, natural mating and pregnancy confirmation via ultrasonography on day 45 post-mating. The ewes were managed according to NRC (2007) dietary guidelines until day 100 of gestation, after which they were assigned to three experimental groups: subclinical PT group (group 1; G1, n = 6), treatment group (subclinical PT + L-carnitine, group 2; G2, n = 6) and control group (group 3; G3, n = 6). Blood beta-hydroxybutyrate (beta HBA) concentrations were measured on day 100 and 138 of the gestation. Then, all ewes were slaughtered for fetal muscle sampling from the Musculus Longissimus Dorsi (MLD) and Vastus Lateralis (VL). Results indicated a significant reduction in muscle fibre number and fibre diameter in both MLD and VL in the SPT group (G1) compared to the control (G3) (p < 0.05). No significant differences were observed between G1 and G2 or between G2 and G3 for these parameters (p > 0.05). On the other hand, large effect sizes for group and pairwise comparisons imply that SPT may negatively affect prenatal muscle development and L-carnitine supports muscle development during the prepartum period. These findings highlight the negative effects of SPT and protective effects of L-carnitine supplementation on fetal skeletal muscle development in ewes with SPT. The observed deficits may negatively impact postnatal growth, survival rates and meat quality. Further investigations are warranted to optimise maternal nutrition strategies and evaluate therapeutic interventions aimed at mitigating the adverse impacts of SPT on fetal muscle development in ruminants. Furthermore, L-carnitine supplementation may be a useful in compensating for the negative effects of SPT.
Norduz Koyunlarında Sütün Kimyasal Yapısı ve Fiziksel Özellikleri
(2023) Koca, Davut; Turgut, Ali Osman; Çetin, Nebi; Üner, Sefa; Gülendağ, Erman; Karagülle, Bahtiyar
Bu çalışmada, Van ili Gürpınar ilçesinde yetiştirilen Norduz koyunlarının orta laktasyon döneminde süt kompozisyon özelliklerinin ortaya konulması amaçlanmıştır. Koyunlar klinik ve subklinik mastitis yönünden değerlendirilmiş ve örnekler yalnızca sağlıklı koyunlardan toplanmıştır. Bu amaçla toplam 104 koyun sütü örneği toplandı. Norduz koyun sütünün kimyasal ve fiziksel özellikleri, süt otoanalizörü kullanılarak belirlenmiştir. Çalışmada Norduz sütünde süt yağı (%), 2.48±0.11; yağsız kuru madde (SNF) (%), 10.76±0,08; süt proteini (%), 5.09±0.04; laktoz (%), 4.79±0.04; pH, 6.93±0.02; iletkenlik (mS/cm), 4.41±0.04; donma noktası (°C),-0.602±0.80; tuz (%), 0.8035±0.66; ve yoğunluk (kg/m3), 1039.73±0.39 olarak belirlenmiştir. Süt yağı ile protein (r=-0.36, p<0.001), süt yağı ile SNF (r=-0.32, p<0.001) ve süt yağı ile laktoz (r=-0.36, p<0.001) arasında negatif ve istatistiksel olarak anlamlı korelasyonlar gözlenmiştir. SNF ile süt proteini (r=0.90, p<0.001), SNF ile tuz (r=0.87, p<0.001) ve SNF ile laktoz (r=0.90, p<0.001) arasında ise pozitif ve anlamlı ilişkiler belirlenmiştir. Özetle, Norduz koyun sütünün protein içeriği Türkiye'de yetiştirilen koyun ırklarının çoğunluğunun protein içeriğinden yüksek olduğu tespit edildi. Bu bulgular Norduz koyunlarının süt bileşiminin arttırılmasına değerli bir katkı yapma potansiyeli taşımaktadır.
Paep Gene Restriction Fragment Length Polymorphism and Its Effects on Milk Composition in Cross-Bred Hamdani Sheep
(2024) Turgut, Ali Osman; Gülendağ, Erman; Koca, Davut; Üner, Sefa
Β-lactoglobulin (βLG) stands as the primary whey protein in ruminant milk, synthesized by mammary gland cells during lactation and encoded by the βLG gene. This study aimed to assess the impact of βLG gene exon II polymorphism on milk composition traits in crossbreed Hamdani sheep. Sheep were examined for clinical diseases and mastitis. Milk and blood samples were only collected from healthy ewes. The composition and physical properties of milk were analyzed using milk autoanalyzer. The βLG gene exon II region's 452 bp PCR products were subjected to RFLP analysis using the RsaI restriction enzyme. Two genotypes, AA and AB, were identified for the βLG gene exon II region, with A and B allele frequencies of 0.7 and 0.3, respectively. Statistical analysis, conducted with Minitab® (Version: 19.2020.2.0), revealed that the AA genotype is associated with a higher milk fat percentage p<0.05). However, no significant genotype effect was observed for other milk composition traits in cross-bred Hamdani sheep. These results suggest that PAEP genotypes could serve as valuable indicators for enhancing milk composition in cross-bred Hamdani sheep through breeding programs.
Serum Anti-Mullerian Hormone Levels During Estrus and Diestrus Phases of the Estrous Cycle and Its Possible Effect on Fertility in Cross-Bred Hamdani Sheep
(Univ Agriculture, Fac veterinary Science, 2024) Turgut, Ali Osman; Koca, Davut
This study aimed to assess the variations in serum Anti-mullerian hormone (AMH) levels during the estrus and diestrus phases of the estrous cycle and its effect on fertility in cross-bred Hamdani ewes. In the study, 21 non-prolific cross-bred Hamdani ewes (aged 2-3 years with BCS 2.75-3.25) were subjected to estrous synchronization using intra-vaginal sponges. Following synchronization, ewes underwent natural mating and pregnancy examination was carried out on the 45(th) day after mating through trans-rectal ultrasonography. Jugular blood samples were collected on day of estrus and 8 days later and analyzed for serum AMH and progesterone concentrations. Results showed that serum AMH levels were 100-480 and 95-520 pg/ml during estrus and diestrus phases, respectively. There was no change in serum AMH levels due to age, BCS and during estrus and diestrus phases of the estrous cycle (P>0.05). Estrus and diestrus serum AMH levels were significantly higher in pregnant ewes compared to non-pregnant ewes (P<0.05). Receiver-operating characteristic (ROC) analysis revealed that serum AMH cut-off value of >270 pg/mL was feasible to predict fertility in cross-bred Hamdani ewes. In conclusion, serum AMH can be used as a marker of fertility in non-prolific cross-bred Hamdani ewes.
Subclinical Pregnancy Toxemia Affects Blood Parameters of Ewes and Impairs Postnatal Growth and Development of Lambs
(Wiley, 2025) Turgut, Ali Osman; Kucuk, Mursel; Irmak, Mehmet; Ozcan, Cahit; Koca, Davut; Gulendag, Erman; Keskin, Ibrahim Halil
Pregnancy toxemia (PT) is a common metabolic disorder that occurs in the last trimester of pregnancy. This study aimed to detect the effects of subclinical PT (SPT) on serum biochemical parameters of ewes and postnatal growth and development of lambs in Romanov sheep. A total of 40 ewes and 69 lambs were included in the study. Blood samples of ewes were collected at the time of parturition to evaluate serum beta-hydroxybutyric acid (beta HBA), serum nonesterified fatty acid (NEFA), cholesterol, and triglyceride. Live weight and body measurements of lambs were recorded at birth (0th day), 30th day, and 60th (weaning) day. In the diagnosis of SPT serum beta HBA concentrations were taken into account. A cut-off value of 0.8 mmol/L for beta HBA was a critical concentration and ewes with 0.8-1.6 mmol/L beta HBA were evaluated as SPT. There were no ewes with Beta HBA value higher than 1.6 mmol/L (clinical PT) in the study. There was no significant difference in serum beta HBA, NEFA and cholesterol concentrations between ewes regarding birth type (single, twin, and triplet). However, triglyceride concentrations were higher (p < 0.001) in ewes giving birth to triplets compared to ewes giving birth to single lamb. Serum beta HBA and triglyceride concentrations were higher (p < 0.01) in ewes with SPT (n = 14) compared to healthy ewes (n = 26). There was no significant difference between ewes with SPT and healthy ewes in serum NEFA and cholesterol. Repeated measures ANOVA results indicated that SPT negatively affected 0th, 30th, and 60th body weights of twin (p < 0.01) and triplet born lambs (p < 0.05). The effect of time on body weights was also found to be significant (p < 0.001). Similarly, most of the body measurements of twin and triplet born lambs were lower (p < 0.05) in the SPT group compared to healthy group. Overall results indicate that SPT may negatively affect postnatal growth and development in Romanov lambs. Because a decline in growth performance has many negative outcomes affecting production traits in sheep, these findings may be valuable for the veterinary field.