Browsing by Author "Turker, Musa"

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Abcisic Acid and Gibberellic Acid Cause Increased Lipid Peroxidation and Fluctuated Antioxidant Defense Systems of Various Tissues in Rats
(Elsevier Science Bv, 2007) Celik, Ismail; Turker, Musa; Tuluce, Yasin
The study was aimed at demonstrating changes in the antioxidant defense systems [Reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT)] forming an antioxidative barrier and oxidative stress parameter (Malondialdehyde = MDA) in the various tissues of Sprague-Dawley rats which were administrated plant growth regulators (PGRs) [Abcisic acid (ABA) and Gibberellic acid (GA(3))] during 25 days. Seventy five parts per million of ABA and GA(3) as drinking water were administered orally to rats (Sprague-Dawley albino) ad libitum for 25 days continuously. The PGRs treatments caused different effects on antioxidant defense systems and MDA content of experimented rats compared to controls. The lipid peroxidation end product MDA significantly increased in the spleen and lungs of rats treated with ABA and GA(3) without significantly change in the other tissues. The GSH levels were significantly increased in the lungs and stomach of rats treated with ABA without any change in the tissues of rats treated with GA(3). Antioxidant enzyme activities such as SOD significantly increased in the spleen of rats treated with ABA and GA(3). Meanwhile, SOD significantly increased in the kidney of rats treated with GA. CAT significantly decreased in the lungs treated with ABA but did not change significantly in all the rest of rat tissues treated with both the PGRs. On the other hand, the ancillary enzyme GR activity decreased in the spleen and increased in the kidney with GA(3) treatment. The drug metabolizing enzyme GST activity significantly decreased in the heart of rats treated with GA(3) but increased in the spleen and lungs of rats treated with both PGRs. The observations presented led us to conclude that administration of subacute ABA and GA(3) promotes lipid peroxidatin content and alters in the antioxidative systems in the rat's various tissues. These data, along with changes, suggest that the PGRs produced oxidative stress in rats during the period of a 25-day subchronic exposure. (C) 2007 Elsevier B.V. All rights reserved.
Antioxidant and Immune Potential Marker Enzymes Assessment in the Various Tissues of Rats Exposed To Indoleacetic Acid and Kinetin: a Drinking Water Study
(Academic Press inc Elsevier Science, 2006) Celik, Ismail; Tuluce, Yasin; Turker, Musa
In the present study, the influence of two different PGRs, indoleacetic acid (IAA) and kinetin (Kn) on immune potential enzymes, adenosine deaminase (ADA) and myeloperoxidase (MPO), and antioxidant defense enzymes such as glutathione peroxidase (GPx), glutathione reductase (GR), and superoxide dismutase (SOD) in various tissues of rats were investigated during the treatment as a drinking water model. 100 ppm of IAA and Kn as drinking water were administered orally to rats (Sprague-Dawley albino) ad libitum for 21 days continuously. The PGRs treatments caused different effects on the immune potential and antioxidant defense enzymes of experimented rats compared to controls. Results show that IAA caused a significant decrease in GR activity in the lungs and liver and an increase in the spleen. Also, IAA caused a significant decline in GPx activity in the lungs and an increase in the heart. SOD was significantly reduced in the heart, while increased in the lungs. Furthermore, IAA caused a significant decrease in ADA activity in the heart and blood whereas an increase in the kidney and spleen. MPO activity was also significantly increased in the heart by IAA treatment. The activity of enzymes were also seriously affected by Kn; GR activity decreased in the lungs, brain, and blood while GPx activity decreased in the spleen, brain, and heart. ADA activity was also significantly reduced in the blood whereas MPO activity rose in the spleen. In addition, SOD activity lowered in all tissues except for lungs where a significant increment was determined. As a conclusion, the results indicate that PGRs might affect on antioxidant and immune potential enzymes. These data, along with the determined changes suggest that PGRs produced substantial systemic organ toxicity in the erythrocyte, liver, brain, heart, lungs, spleen, and kidney during the period of a 21-day subacute exposure. (c) 2006 Elsevier Inc. All rights reserved.
Antioxidant Capacity and Phenolic Constituents of Malva Neglecta Wallr. and Plantago Lanceolata L. From Eastern Anatolia Region of Turkey
(Elsevier Gmbh, 2012) Dalar, Abdullah; Turker, Musa; Konczak, Izabela
Plantago lanceolata and Malva neglecta are two plants from eastern regions of Turkey used by the local population to cure multiple medical conditions, such as promoting maturation of abscesses, wound healing, abdominal pains and cancer. Both plants exhibited high oxygen radical absorbance capacity (ORAC assay) and total reducing capacity (ferric reducing antioxidant power, FRAP assay), comparable to or higher than herbs such as rosehip, cinnamon or oregano and multiple Chinese medicinal plants. Phenolic compounds were the major constituents of hydrophilic extracts and their levels correlated well with antioxidant activities. Leaf and flower/fruit, which are predominantly used as a medicine, contained the highest levels of phenolics, and comprised predominantly flavonoids and hydroxycinnamic acids. This may suggest that flavonoids and hydroxycinnamic acids play an essential role as physiologically active constituents of these traditional medicinal plants. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.
Bitki Doku Kültürlerinde Sekonder Metabolit Miktarını Arttırmaya Yönelik Uygulamalar
(2021) Turker, Musa; Vuran, Neşe Eray
Doku kültürü teknikleri, 1900’lü yılların başında uygulanmaya başlanmış ve bu oldukça ümit verici bulunmuştur. Bitkidenalınan tek bir parçadan yeni bitkilerin çok kısa sürede, arzu edilen sayıda, dış şartlara bağımlı olmaksızın üretilebileceği fikribilim adamlarını heyecanlandırmıştır. Ancak yapılan çalışmalarda her bitki türü için sistemin optimizasyona gerek duyması,bazı genotiplerin doku kültüründe iyi cevap verirken bazılarının gelişimlerinin oldukça kısır kalması, yüksek yapılı bitkilerdeise başarının sağlanamaması bilim adamlarını doku kültürünü farklı amaçlarla kullanma yoluna sevk etmiştir. Bu yollardan birive belki de en önemlisi değerli fitokimyasalların doku kültüründe üretimidir. Doku kültüründe gelişen bitkiler çevresel şartlarlasınırlandırılmaz ve uygun bir kültür ortamı sağlanmasıyla istenilen bileşiklerin biyosentezi yapılabilir ve bu bileşiklerin miktarıarttırılabilir. Sekonder metabolitlerin doku kültürü ortamında üretilmesiyle arz talep dengesine dayanan, çevresel etkilerdenbağımsız üretim sağlanabilir. Sabit kararlılıkta, belli bir standardı olan maddeler üretilebilir. Doğa tahribatı en aza indirilip,daha az arazi kullanımının gerçekleşmesi sağlanabilir. Yeni sekonder metabolitlerin eldesi mümkün olabilir. Nesli tükenmetehlikesi altındaki türler korunabilir. Bileşenlerin biyosentez yollarının aydınlatılmasında, değiştirilmesinde, sekondermetabolitlerin üretimi ve çeşitli etkenlerle miktar arttırılmasında, iyi ürün veren türlerin seleksiyonunda bitki doku kültürleriumut vaat etmektedir.
Centaurea Karduchorum Boiss. From Eastern Anatolia: Phenolic Composition, Antioxidant and Enzyme Inhibitory Activities
(Elsevier Gmbh, Urban & Fischer verlag, 2015) Dalar, Abdullah; Uzun, Yusuf; Mukemre, Muzaffer; Turker, Musa; Konczak, Izabela
The phenolic composition of lyophilized hydrophilic extracts obtained from root, stem, leaf and flower of Centaurea karduchorum Boiss. a medicinal plant endemic to Eastern Anatolia (Turkey) used as antidiabetic agent was characterized for the first time. Liquid chromatography mass spectrometry (LC/MS) analysis revealed that luteolin derivatives and chlorogenic acid were the dominating compounds contributing, respectively, from 16.1% (root) to 33.7% (stem) and from 7.3% (flower) to 12.0% (root) of total phenolics. Total reducing capacities of the extracts varied from 24.1 (root) to 38.5 (flower) mg gallic acid Eq./g DW [Folin-Ciocalteu assay] and from 274.0 (root) to 441.0 (stem) mu mol Fe2+/g DW [FRAP assay]. The oxygen radical absorbance capacity (ORAC assay) values varied from 930.5 (root) to 1853.5 (stem) mu mol trolox Eq./g DW. The leaf extract exhibited a pronounced inhibitory activity towards alpha-glucosidase (IC50: 0.63 +/- 0.00 mg/ml) and a weak inhibitory activity towards amylase (IC50: 14.63 +/- 0.67 mg/ml), which suggests a potential to reduce postprandial hyperglycaemia and supports its traditional use as antidiabetic agent. (C) 2015 Elsevier GmbH. All rights reserved.
The Effects of Abiotic Stressors and Signal Molecules on Phenolic Composition and Antioxidant Activities of in Vitro Regenerated Hypericum Perforatum (St. John's Wort)
(Elsevier, 2020) Eray, Nese; Dalar, Abdullah; Turker, Musa
Hypericum perforatum L. is a pharmaceutically and industrially significant natural source due to its rich phenolic content and antioxidant potential. This study aimed to establish an optimized development protocol through plant tissue culture methods and evaluate the effects of abiotic stressors and signal molecules on phenolic composition and antioxidant responses. Methanol based extracts prepared from lyophilized powders of plant samples obtained from nature and aseptic seedlings, and calluses obtained from abiotic stress factors and signal molecules were analysed for phenolic composition using HPLC-MS/MS and antioxidant responses through complementary methods (FCR, FRAP and ORAC). Experimental analysis revealed the use of B5 and SH media for in vitro plant regeneration as the most proper chemical environments for the development and standardization of Hypericum perforatum. Chromatographic findings showed that quercetin, pseudohypericin and partially chlorogenic acid compounds as key phenolic compounds of calluses and plant samples of Hypericum perforatum. The analyses deduced that UV and sucrose increased the level of chlorogenic acid, while ascorbic acid increased the level of quercetin and drought applications increased the concentration of pseudohypericin compared to the control. Rutin compound was only produced in pot samples, which indicates a significant response to the drought stress. Additionally, it was found that B5, SH, and MS media containing of 2 mg/L NAA+1 mg BA with elicitors of 50 mu mol of ascorbic acid and 6% sucrose and a UV stress of 5 (or 10) min-24 h harvesting might be a proper protocol for the optimization and standardization of Hypericum perforatum L. Findings obtained within this study revealed novel contributions for optimization, standardization and production of antioxidative phenolic compounds of Hypericum perforatum plant samples for pharmaceutical and industrial utilization. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.
The Effects of an Artificial and Static Magnetic Field on Plant Growth, Chlorophyll and Phytohormone Levels in Maize and Sunflower Plants
(Ferdinand Berger Soehne, 2007) Turker, Musa; Temirci, Cabir; Battal, Peyami; Erez, Mehmet E.
In the present study the effects of a continuous static magnetic field (SMF) on growth and concentration of phytohormones and chlorophylls were investigated in maize and sunflower plants. SMF was applied in two directions; parallel to gravity force (field-down) and anti-parallel (field-up). Chlorophyll concentrations decreased in maize plants, but increased in sunflower in SMF of either direction. Root dry weight decreased in maize and increased in sunflower plants. The changes of dry weight in stem and leaf were not significant (p >= 0.05). The root length decreased in both plant species. Leaf and stem length increased in maize plants in SMF of either direction. Leaf length did not change in sunflower, whereas stem length rose in field-down application of SME Concentrations of gibberellic acid-equivalents (GAs), indole-3-acetic acid (IAA) and trans-zeatin (t-Z) increased in sunflower plants under field-up application of SMF whereas they decreased in SMF of the opposite direction. The concentration of phytohormones decreased in maize plants in SMF of either direction.
Fatty Acid, Sugar and Vitamin Contents in Rose Hip Species
(Asian Journal of Chemistry, 2008) Yoruk, I. Hakki; Turker, Musa; Kazankaya, Ahmet; Erez, M. Emre; Battal, Peyami; Celik, Ferit
The content of oleic and linoleic acids, glucose, fructose, sucrose, maltose, vitamin C and E (alpha and delta-tocopherol) was determined in seeds and fruits of Rosa iberice, Rosa canina, Rosa villosa, Rosa dumalis and Rosa pisiformis. The highest level of the compounds in fruits was determined as follows: linoleic acid in R. dumalis (3.150 mu g/g), oleic acid in R. canina (0.57 mu g/g), delta-tocopherol in R. dumalis (10. 12 mu g/g), alpha-tocopherol in R. pisiformis (17.60 mu g/g), vitamin C in R. canina (2855.33 mu g/g), fructose in R. dumalis (18.44 mg/g), glucose in R. dumalis (10.04 mg/g), sucrose in R. canina (5.61 mg/g) and maltose in R. dumalis (1.92 mg/g). The highest level of the compound in seeds was also determined and sequenced as follows: linoleic acid in R. canina (3.97 mu g/g), oleic acid in R. dumalis (10.50 mu g/g), delta-tocopherol in R. canina (7.15 mu g/g), alpha-tocopherol in R. iberice (11.01 mu g/g), vitamin C in R. iberice (952.10 mu g/g), fructose in R. pisiformis (17.20 mg/g), glucose in R. iberice (9.83 mg/g), sucrose in R. dumalis (14.96 mg/g) and maltose in R. canina (2.46 mg/g).
In Vitro Antioxidant and Enzyme Inhibitory Properties and Phenolic Composition of M. Neglecta Wallr. (Malvaceae) Fruit: a Traditional Medicinal Fruit From Eastern Anatolia
(Elsevier, 2013) Turker, Musa; Dalar, Abdullah
Malva neglecta Wallr. (Malvaceae) fruit is an edible traditional medicinal plant used in Eastern Anatolia to cure multiple medical conditions such as asthma, stomach ache, diarrhoea, etc. In this study, lyophilized ethanol-based hydrophilic extract was investigated for total phenolic content and phenolic composition using liquid chromatography coupled with mass spectrometry, in vitro antioxidant capacity (FCR, FRAP and ORAC assays) and enzyme inhibitory activities. Ethanol-based hydrophilic extract prepared from M. neglecta fruit was identified as a rich source of phenolic compounds, exhibited pronounced Folin-Ciocalteu reducing activity (12.8 +/- 0.7 mg gallic acid Eq./g DW), ferric reducing antioxidant power (174.3 +/- 9.8 mu mol Fe2+/g DW) and oxygen radical scavenging activity (1656.9 +/- 69.7 mu mol Trolox Eq./g DW) and weakly suppressed the activity of alpha-amylase (IC50: 15.2 +/- 0.8 mg/ml), a-glucosidase (IC50: 14.19 +/- 0.10 mg/ml) and pancreatic lipase (IC50: 17.55 +/- 0.20 mg/ml). Flavonoid glycosides were tentatively detected as the major phenolic compounds. Furthermore, traces of rutin, chlorogenic acid, hydroxybenzoic acid and hydroxybenzoic acid-O-hexoside were also detected in the extract. High levels of antioxidant capacities and complex mixture of phenolic compounds of M. neglecta fruit revealed that this fruit might be a good source for natural antioxidants. (C) 2013 Elsevier B.V. All rights reserved.
In Vitro Multiple Shoot Regeneration From Petunia Hybrida
(2019) Özdemir, Fethi Ahmet; Turker, Musa; Habas, Rebaz Resul
An efficient plant regeneration protocol was developed from in vitro germinated seeds of Petuniahybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants ofPetunia hybrida were cultured on MS basal medium supplemented with different concentrations andcombinations of Benzyl amino purine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-butyricacid (IBA) and Gibberellic acid (GA3). The highest shoot length was obtained from MS mediumsupplemented with 1 mg/l BAP + 1 mg/l NAA. The highest shoot number (3 shoots/explant) wereobtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA. The isolated shootswere transferred to MS basal medium supplemented with different concentrations of GA3 rangingfrom 0.05, 0.2, 0.5 and 1 mg/l for shoot elongation. The highest shoot length (5.75 cm) was recordedfrom the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP. Rooting of regeneratedshoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA. The regeneratedshoots with well developed roots were successfully acclimatized and established in pots containingsterilized peat moss and grown under laboratory conditions with 70% survival rates.
In Vitro Propagation and Secondary Metabolites Investigation of Hypericum Perforatum L
(Parlar Scientific Publications (p S P), 2019) Mohammed, Namam Rebaz; Dalar, Abdullah; Ozdemir, Fethi Ahmet; Turker, Musa
Hypericum perforatum L. was regenerated in plant tissue culture and secondary metabolites (hypericin, pseudohypericin, quercetin, rutin, and chlorogenic acid) of the plants collected from field and regenerated in vitro were quantitatively compared. The liquid, semi solid and solid form of Murashige and Skoog (MS) basal medium supplemented with plant growth regulators (PGRs) in different concentration and combination were employed for regeneration and secondary metabolite product amplification. Based on preliminary tests nodal segment was preferred as an explant. At the end of 50 days of regeneration period, no statistically significant difference was observed between the length of root and shoot and root number of plants regenerated in solid, semi solid and liquid media supplemented with different PGRs. The quantitative secondary metabolite analyses was performed by High Performance Liquid Chromatography (HPLC). The highest concentrations of chlorogenic acid, quercetin, and pseudohypericin were observed in shoots and roots of the plants collected from field. Whereas the compounds were detected in low quantity in plants regenerated in the liquid, semi solid and solid medium, except for quercetin which was found higher concentration than chlorogenic acid, quercetin, and pseudohypericin in vitro regenerated plants. Hypericin and rutin were not detected by HPLC analysis in all of plants regenerated in vitro and in vivo.
The Investigation of The Medicinal Potential of Alcea Kurdica Alef. in Nature and Tissue Culture
(Soc Stiinte Farmaceutice Romania, 2022) Gormez, Gul; Battal, Abdulhamit; Dalar, Abdullah; Turker, Musa
Alcea kurdica Alef. (AK) a hollyhock has been used in folk medicine to treat various diseases. Though its extensive utilization in traditional medicine, experimental studies regarding to its phytochemical composition is limited. The aim of this study was to determine the antioxidant capacity and phenolic composition of ethanol extract of AK plant collected from the natural environment in different vegetation periods (June-flowering, July-fruiting, August-seeding) and to control its antioxidant capacity and phenolic composition by in vitro shoot tip culture. AK plant at the flowering period had the highest antioxidant capacity, while the lowest activity was determined at the seeding period according to results. Phenolic acids dominated by gallic, chlorogenic and caffeic acids were determined as the major phenolics. According to in vitro results, the use of 1-naphthylacetic acid (NAA) and 6-benzylaminopurine (BA) alone caused significant decrease in the three phenolic acids content compared to the control. On the other hand, combination of NAA and BA caused significant increase in phenolic acids content compared to the control. Moreover, significant increase in ORAC was observed in AK extract obtained from in vitro Murashige & Skoog (MS) media culture supplemented with NAA and BA combination. 15 min of UV exposure negatively affected the antioxidant capacity and phenolic content.
Molecular and Physiological Changes in Maize (Zea Mays) Induced by Exogenous Naa, Aba and Meja During Cold Stress
(Finnish Zoological Botanical Publishing Board, 2008) Battal, Peyami; Erez, Mehmet E.; Turker, Musa; Berber, Ismet
Different concentrations of abscisic acid (ABA), naphthalene acetic acid (NAA) and methyl jasmonate (MeJa) were applied to maize (Zea mays) plants exposed to cold stress. ABA enhanced plant resistance against the damaging effects of freezing. ABA-treated plants produced some specific proteins when exposed to cold. Prominent changes were observed in the size and number of stomata with the application of ABA during cold stress. MeJa inhibited root growth, but increased root dry weight during cold acclimation. The chlorophyll content was lower at 100 mu g ml(-1) NAA and 50 mu g ml(-1) MeJa and higher in the residual applications when compared with that in the control plants. Carotenoid levels increased in all NAA and some ABA treatments, decreased however in MeJa treatment. Exogenous MeJa treatments increased endogenous trans-zeatin (t-Z) and indole-3-acetic acide (IAA) in contrast decreased ABA levels. Exogenous NAA also increased endogenous IAA, ABA and zeatin riboside (ZR) levels, while exogenous ABA decreased endogenous IAA and ZR levels.
Phenolic Composition, Antioxidant and Enzyme Inhibitory Activities of Eryngium Bornmuelleri Leaf
(Springer, 2014) Dalar, Abdullah; Turker, Musa; Zabaras, Dimitrios; Konczak, Izabela
Eryngium bornmuelleri Nab. (TusI) is an endemic botanical from the Eastern Anatolia region of Turkey traditionally used for preparation of herbal tea. Within this study, phenolic composition, antioxidant capacities and inhibitory activities towards selected digestive enzymes of E. bornmuelleri leaf were investigated. Sequential extracts, obtained by extraction of plant tissue by ethanol, acetone and water exhibited pronounced antioxidant capacities and in a dose-dependent manner suppressed the metabolic syndrome related enzymes: alpha-amylase, alpha-glucosidase and pancreatic lipase. All extracts contained high levels of phenolic compounds. Flavonoid glycosides were the main phytochemicals detected, with rutin as the major compound (70 % of total phenolics). Chlorogenic, hydroxybenzoic and caftaric acids as well as traces of caffeic, ferulic and rosmarinic acids were also detected. Correlation analysis indicated that phenolic compounds were the major sources of the enzyme-inhibitory activities. This study suggests that E. bornmuelleri leaf extracts can modulate the metabolism of sugars and fats through inhibition of the relevant digestive enzymes.
Phenolic Compound Production Increased in Vitro Regenerated Cichorium Intybus L
(Mdpi, 2023) Abas, Yousif Abdullah; Eroglu, Ayten; Dalar, Abdullah; Turker, Musa; Ozdemir, Fethi Ahmet; Solowski, Gawel
Chicory (Cichorium intybus L.) is a low-height perennial or biennial herb from the family of Asteraceae. Investigation of different in vitro regeneration strategies of Cichorium intybus and increasing the number of secondary metabolites in vitro regenerated plant samples were the aims of the research. Callus and plant regenerations were achieved in basal plant growth media supplemented with plant growth regulators (PGRs). Whole plant regeneration was carried out by direct organogenesis from leaf explant in Murashige and Skoog (MS) and B5 media supplemented with naphthalene acetic (NAA) acid and indole-3-butyric acid (IBA). The highest callus quantity was produced in MS medium supplemented with indole-3-acetic acid (IAA) and benzyl amino purine (BAP). The combination and concentrations of PGRs used in MS and B5 media not only provided root and shoot formation with callus, but also caused a change in the amounts of phenolic components. In addition, some PGRs used caused an increase in the number of phenolic compounds in callus and shoots developed from the leaf explant. When plants that grow in vitro and in vivo are compared with each other, it has been determined that plants grown in vivo contain higher amounts of some phenolic compounds. In vivo and in vitro samples were extracted in ethanol/water (80:20 v/v). The analysis of phenolic compounds (caftaric, chicoric, and chlorogenic acids and esculin) were performed in high-performance liquid chromatography (HPLC) and inulin was in UV spectrophotometry. The caftaric and chlorogenic acids and inulin concentrations were higher in vivo samples than that in vitro. Contrarily, esculin, and chicoric acid concentrations were higher in the in vitro regenerated samples. The higher concentration of valuable compounds in the in vitro regenerated samples, especially in callus tissue, gives hope for large-scale production of secondary metabolites under laboratory conditions.
Phytohormone Levels in Germinating Seeds of Zea Mays L. Exposed To Selenium and Aflatoxines
(Springer, 2006) Agar, Guleray; Turker, Musa; Battal, Peyami; Erez, M. Emre
Seeds of Zea mays L. were exposed to allatoxine B1 (AFB1), aflatoxine G1 (AFG1) and selenium (Se) alone and in combination and allowed to germinate. Phytohormone levels of GA-like substances (GAs), trans-Zeatin (t-Z) and Indole-3-acetic acid (IAA) were determined by High Performance Liquid Chromatography (HPLC) when the roots of the germinating seeds reach 1.5-3.0 cm in length. The levels of endogenous hormones decreased in seeds treated with AFB1 and AFG1 compared to control; however an increase was noted in seeds exposed to AFG1 and Se together. AFB1 and Se treatment caused reduced hormone levels in most of the treatments. When plants were exposed to Se alone, the highest levels of GAs, t-Z and IAA were observed in the application of 800 ppm Se. The highest levels of GAs, t-Z and IAA were observed when seeds were treated with 0.2 ppm AFG1 + 8 ppm Se, 0.2 ppm AFG1 + 8 ppm Se and 0.2 ppm AFG1 + 0.08 ppm Se, respectively, whereas the lowest levels of the hormones were observed in 0.2 ppm AFB1 + 8 ppm Se, 0.2 ppm AFB1 + 0.08 ppm Se and 0.1 ppm AFB1, respectively. In conclusion, the levels of phytohormones were reduced by the treatment of AFB1 and AFG1 alone. However Se removed the negative effect of AFB1 on phytohormones, but not AFB1.
Tıbbi Öneme Sahip Hatmiler İçin Etkili Bir Çimlendirme Yöntemi: Tohum Kabuğuna Çentik Atılması
(2019) Görmez, Gül; Turker, Musa; Battal, Abdulhamit
Malvaceae familyasının üyelerinden biri olan Alcea cinsi tıbbi öneme sahip bazı türleri içermektedir. Alcea kurdicaAlef. (Deve gülü Hatmi) tonsilite, gastrik ülsere, zatürreye, boşaltım sistemi enfeksiyonlarına ve saç kırana karşıkullanılan tıbbi bir bitkidir. Bu çalışmanın amacı, deve gülü hatmi bitkisi için steril şartlar altında etkili birçimlendirme yönteminin geliştirilmesi ve bu bitkinin doku kültürü çalışmalarında kullanılabilir hale getirilmesidir.Bu amaç için, steril bir ajan olan sodyum hipokloritin üç farklı konsantrasyonu (%5, %7.5 ve %10), 42°C sıcaklıktainkübasyonu ve tohum kabuğunun bistüri ile çentiklenmesi uygulamaları test edilmiştir. %5 ve %7.5 sodyumhipoklorit konsantrasyonlarının sterilizasyon için yeterli olmadığı belirlenirken, %10 sodyum hipokloritkonsantrasyonun ise yeterli olduğu gözlenmiştir. Çimlenme oranları sonuçlarına göre, kabuğu çentiklenentohumlar hızlıca çimlenirken, 42°C de inkübasyonun çimlenmeyi etkilemediği gözlenmiştir. Yedinci günde,kotiledon, kotiledon sapı ve hipokotil dokuları doku kültürü için kullanılabilir duruma gelmiştir. Sekizinci günde,yapraklar oluşmaya başlamıştır. Yaprak, yaprak sapı ve sürgün ucu eksplantları ise otuzuncu günde dokukültüründe kullanılabilecek duruma gelmiştir. Sonuç olarak, bu çalışma ile tıbbi öneme sahip deve gülü hatmi içinetkili bir çimlendirme yöntemi geliştirilmiştir.
Total Nitrogen Content of Cucumber (Cucumis Sativus L.) Calluses
(Elsevier Science Bv, 2016) Turker, Musa; Eray, Nese; Gormez, Gul
Β-Carboline Production From in Vitro Regenerated Peganum Harmala L. Plant
(Parlar Scientific Publications (p S P), 2018) Onlu, Seyma; Bingol, Omer; Turker, Musa; Ozdemir, Fethi Ahmet
Peganum harmala L. seeds were germinated in hormone free sterile MS medium. Root, shoot tip, leaf, petiole, hypocotyl, cotyledon and nod explants excised from in vitro regenerated plants were incubated in MS medium supplemented with 1 mg/l and 2 mg/l 2,4-D for callus production. Hypocotyl and cotyledon explants produced callus and untreated callus was accepted as control for the comparison of beta-carboline alkaloids production extracted from different samples. Callus were exposed to cold and dark conditions and incubated in MS medium supplemented with 2 mg/l ABA, 1 mg/l ABA, 1 mg/l NAA and 1 mg/l BAP in different batches to increase beta-carboline alkaloid production. Seeds were germinated in soil ambient condition. beta-carboline alkaloids; harmalol, harmine and harmaline were extracted from seeds, plants grown in soil, callus grown in MS medium supplemented with 2,4-D, callus incubated MS medium supplemented with NAA, BAP, ABA and callus exposed cold and dark condition. The extracts were quantitatively analysed with HPLC. The highest alkaloid concentrations were determined in vivo seeds and plants grown in soil. However, alkaloid production in callus tissues is also promising. Harmalol and harmine concentration decreased with cold treatment but increased with ABA treatment compared to that of the control. Harmaline was increased with cold treatment, but dramatically decreased in all treatment that callus is exposed.