Browsing by Author "Turker, R.S."

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Analysis of Phytochemical Composition and Biological Activities of Helichrysum Pallasii (Sprengel) Ledeb Leaves
(Pamukkale University, 2023) Işık, N.; Mukemre, M.; Turker, R.S.; Zengin, G.; Dalar, A.
This study aimed at the investigation of in vitro biological activities (antioxidant and enzyme inhibition) and phytochemical composition of various extracts (ethanol maceration, aqueous infusion, and aqueous decoction) obtained from leaf organs of Helichrysum pallasii, which has been traditionally used as herbal tea and medicine in Eastern Anatolia. Experimental analysis showed that ethanol-based extract had higher total phenolic content (TPC: 103 mg GAE/gE) and showed superior antioxidant potentials (FRAP: 2205 μmol Fe2+/gE; ORAC: 2540 μmol Trolox Eq./gE; DPPH: IC50=0.58 mg/ml; CUPRAC: IC50=0.37 mg/ml; Phosphomolybdenum: IC50=1.34 mg/ml ve metal chelation: IC50=1.42 mg/ml) and enzyme inhibition (Acetylcholinesterase: IC50=1.49 mg/ml; Butyrylcholinesterase: IC50=1.98 mg/ml; Tyrosinase: IC50=0.68 mg/ml; Alpha-amylase: IC50=2.09 mg/ml; Alpha-glucosidase: IC50=0.51 mg/ml; and Pancreatic lipase: IC50=42.5 μg/ml) and contained higher amounts of phenolic (chlorogenic acid isomers and rutin) and fatty acid (palmitic, linoleic, and linolenic acids) compounds than traditional preparations (infusion and decoction). The current study's findings indicate that the leaves of the Helichrysum pallasii are a source of phytochemicals with strong antioxidant and enzyme inhibitory properties, implying that it could be a candidate for biotherapeutic agent research and development. © 2023 The authors.
Biological Activities and Chemical Composition of Xanthoria Lichens From Turkey
(Pamukkale University, 2021) Mukemre, M.; Zengin, G.; Turker, R.S.; Aslan, A.; Dalar, A.
This study presents the biopharmaceutical potential and bioactive composition of Xanthoria lichens (X. candelaria, X. elegans, X. parietina) that wildly grown and traditionally utilized as medicine in North Eastern Anatolia, Turkey, which has specific microclimatic and ecological zones. Chromatographic findings revealed significant levels of parietin compound (35 to 49 mg/g extract), low levels of various fatty acids and a volatile compound; α-terpinene in the extracts. The extracts exhibited pronounced antioxidant potential through reducing and scavenging mechanisms; FCR: 33-38 mg gallic acid equivalent, FRAP: 511-815 μ mol Fe2+, ORAC: 1032-1355 μ mol Trolox equivalent per gram extract, respectively and DPPH: IC50: 1.1-2.7, ABTS: IC50: 2-2.3, CUPRAC: IC50: 0.7-1.2, phosphomolybdenum: IC50: 2-2.9, metal chelation: IC50: 1.3-2.3 mg extract/ml, respectively. Concerning enzyme inhibitory activities, the extracts effectively suppressed the activity of acetylcholinesterase (IC50: 0.5-0.75 mg/ml), butyrylcholinesterase (IC50: 0.7-1.1 mg/ml), tyrosinase (IC50: 0.6-0.7 mg/ml), amylase (IC50: 1.7-2 mg/ml), glucosidase (IC50: 0.6-3 mg/ml) and lipase (IC50: 55-79 μg/ml) enzymes. These findings showed that Xanthoria lichens are dominated by parietin as the major key compound and high-tolerated lichen taxa towards to different ecological and climatic conditions. These lichens might be promising sources of novel antioxidant and enzyme inhibitory activities such as Xanthoria candelaria as antioxidant and antilipase, Xanthoria elegans as anticholinesterase, and Xanthoria parietina as antiamylase and antiglucosidase. © 2021 Acta Biomedica Scientifica. All rights reserved.