Browsing by Author "Tuzun, Sefa"

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Human Vascular Adhesion Protein-1 (vap-1): Serum Levels for Hepatocellular Carcinoma in Non-Alcoholic and Alcoholic Fatty Liver Disease
(Biomed Central Ltd, 2010) Kemik, Ozgur; Sumer, Aziz; Kemik, Ahu Sarbay; Itik, Veyis; Dulger, Ahmet Cumhur; Purisa, Sevim; Tuzun, Sefa
Background: The incidence of hepatocellular cancer in complicated alcoholic and non-alcoholic fatty liver diseases is on the rise in western countries as well in our country. Vascular adhesion protein-1 (VAP-1) levels have been presented as new marker. In our study protocol, we assessed the value of this serum protein, as a newly postulant biomarker for hepatocellular cancer in patients with a history of alcoholic and non-alcoholic fatty liver diseases. Methods: Pre-operative serum samples from 55 patients with hepatocellular cancer with a history of alcoholic and non-alcoholic fatty liver diseases and patients with cirrhosis were assessed by a quantitative sandwich ELISA using anti-VAP-1 mAbs. This technique is used to determine the levels of soluble VAP-1 (sVAP-1) in the serum. Results: sVAP-1 levels were evaluated in patients with hepatocellular cancer and liver cirrhosis. There was a significant difference in mean VAP-1 levels between groups. Serum VAP-1 levels were found higher in patients with hepatocellular cancer. Conclusion: These findings indicate that the serum level of sVAP-1 might be a beneficial marker of disease activity in chronic liver diseases.
The Levels of the D-Dimer and All Haemostatic Factors in Patients With and Without Deep Venous Thrombosis
(Yerkure Tanitim & Yayincilik Hizmetleri A S, 2010) Kemik, Ozgur; Sumer, Aziz; Kemik, Ahu Sarbay; Purisa, Sevim; Tuzun, Sefa; Kotan, Cetin
Objective: Although improved diagnostic tools and treatment approach, deep venous thrombosis is still an important cause of morbidity and mortality. Deep venous thrombosis is a common condition that affects more than 250 000 patients each year in the United States. In spite of limitations of the available diagnostic tools for detecting deep venous thrombosis, laboratory tests such as plasma D-dimer has been gained much interest recently. So, we aimed to investigate the D-dimer and all hemostatic factors in patients with deep vein thrombosis. Material and Methods: We investigated the levels of the D-dimer and Von Willebrand factor by enzyme linked immunosorbent assay (ELISA) in 120 patients with deep venous thrombosis and in 115 patients without deep venous thrombosis. We used standard methods for detecting the levels of the cholesterol, creatinin, INR, factor VIII: C ratio, and aPTT. We used Clauss method for detecting the fibrinogen levels. Results: We obtained important results in patients with deep venous thrombosis compared to in patients without deep vein thrombosis. Age, BMI, aPTT, INR, and total cholesterol levels were not found statistically differences in patients with deep venous thrombosis (p> 0,05). Plasma D-dimer, creatinin, factor VII: C, and Von Willebrand factor levels were found higher in patients with deep venous thrombosis than in patients without deep venous thrombosis (respectively, p<0,001, p<0,01). But, plasma fibrinogen levels were not found lower in patients with deep venous thrombosis (p<0,01). Conclusion: In conclusion, we suggested that the plasma D-dimer and hemostatic factor levels may play an important role in diagnosis and treatment of deep venous thrombosis.
Lipid Peroxidation and Transforming Growth Factor-Β1 Levels in Gastric Cancer at Pathologic Stages
(Galenos Publ House, 2012) Tuzun, Sefa; Yucel, Ahmet Fikret; Pergel, Ahmet; Kemik, Ahu Sarbay; Kemik, Ozgur
Objective: High levels of TGF-beta 1 and enhanced TGF-beta 1 receptor signaling are related to the pathology of gastric cancer. This effect is caused by oxidative stress and lipid peroxidation products. The aim of this study was to investigate the levels of TGF-beta 1 and lipid peroxidation products in gastric cancer patients and their correlation with pathologic stage. Material and Methods: Lipid peroxidation products and TGF-beta 1 levels were studied in the serum samples of 50 gastric cancer patients and 18 control subjects. Results: HNE-protein adducts and TGF-beta 1 levels were significantly higher in T2, T3 and T4 gastric cancers than in either the T1 stage or controls (p<0.001). Pathologic stage was correlated with TGF-beta 1 levels (r=0.702, p<0.05). Conclusion: These markers production may contribute to tumor angiogenesis and aid in the prognosis of the gastric cancer.
Preoperative Serum Placenta Growth Factor Level as a New Marker for Stage Ii or Iii Colorectal Cancer Patients
(Turkish Soc Gastroenterology, 2012) Kemik, Ozgur; Kemik, Ahu Sarbay; Sumer, Aziz; Purisa, Sevim; Tukenmez, Baris; Tuzun, Sefa
Background/aims: We first reported in this study that serum placenta growth factor and carcinoembryonic antigen in combination were useful markers for selecting early-stage colorectal cancer patients. The aim of the present study was to determine whether serum placenta growth factor could provide carcinoembryonic antigen-independent prognostic information on patients undergoing curative surgery. Methods: Serum and tissue samples were collected from 158 patients with colorectal cancer and from 50 controls. Serum and tissue levels of placenta growth factor were measured by enzyme-linked immunosorbent assay. The serum placenta growth factor levels in colorectal cancer patients were compared with those in healthy controls, and we retrospectively assessed the association between serum placenta growth factor levels and clinicopathological findings and survival. Results: Expression of placenta growth factor was significantly higher in colorectal cancer tissues compared with non-tumor tissues. The mean serum placenta growth factor level in patients was significantly higher than that in controls and significantly higher in patients with large tumor, lymph-node involvement and distant metastasis. Conclusions: Elevated serum placenta growth factor levels are significantly associated with colorectal cancer development, lymph or distant invasive phenotypes and survival, especially in stage II or III patients.
Preoperative Vascular Endothelial Growth Factor Levels as a Prognostic Marker for Stage Ii or Iii Colorectal Cancer Patients
(Libertas Acad, 2011) Kemik, Ozgur; Kemik, Ahu Sarbay; Sumer, Aziz; Purisa, Sevim; Dulger, A. Cumhur; Begenik, Huseyin; Tuzun, Sefa
Background: The aim of the present study was to determine whether serum vascular endothelial growth factor (VEGF) can provide prognostic information independent of carcinoembryonic antigen levels in patients undergoing curative surgery. Methods: Serum samples were collected from 158 patients with colorectal cancer and from 100 controls. Serum and tissue levels of VEGF were measured by enzyme-linked immunosorbent assay. Serum VEGF levels in colorectal cancer patients were compared with those in healthy controls, and we retrospectively assessed the association between serum VEGF levels and clinicopathologic findings and survival. Results: VEGF expression was significantly higher in colorectal cancer tissue compared with nontumor tissue. Mean serum VEGF levels in patients were significantly higher than those in controls, and significantly higher in patients with large tumors, lymph node involvement, and distant metastases. Conclusion: Elevated serum VEGF was significantly associated with poor survival, but was only an independent risk factor for poor survival in Stage II and/or III disease. Elevated serum VEGF is significantly associated with development of colorectal cancer, and lymph or distant invasive phenotypes and survival, especially in Stage II and III patients.
The Relationship Among Acute-Phase Response Proteins, Cytokines and Hormones in Cachectic Patients With Colon Cancer
(Bmc, 2010) Kemik, Ozgur; Sumer, Aziz; Kemik, Ahu Sarbay; Hasirci, Ismail; Purisa, Sevim; Dulger, Ahmet Cumhur; Tuzun, Sefa
Backgraund: Acute-phase response proteins (APRP), cytokines and hormones have been claimed to be an independent prognostic factor of malignancies, however the basis for their association with prognosis remains unexplained. We suggest that in colon malignancies, as similar to pancreatic and lung cancers, changes in APRP are associated with angiogenesis. Methods: C-reactive protein (CRP), albumin, IL-1 alpha, IL-1 beta, IL-6, IL-8, IL-10, TNF-alpha, midkine, VEGF-A, VEGF-C, leptin, adiponectin, and ghrelin serum levels are studied in 126 colon cancer patients and 36 healthy subjects. Results: We found statistically significant difference and correlations between two groups. We found significantly higher serum CRP, IL-1 alpha, IL-1 beta,IL-6, IL-8, IL-10, TNF-alpha, VEGF-A, VEGF-C and leptin concentrations in patients relative to controls (p < 0.001). We found lower levels of the serum albumin, midkine, adiponectin and ghrelin in patients compared to control subjects (p < 0.001). Conclusions: Cachexia in patients with colon cancers is associated with changes in APRP, cytokines and hormone concentrations. These biomarkers and cachexia together have a direct relationship with accelerated angiogenesis. This may lead to a connection between the outcomes in malignancies and the biomarkers.
Serum Procarboxypeptidase a and Carboxypeptidase a Levels in Pancreatic Disease
(Sage Publications Ltd, 2012) Kemik, Ozgur; Kemik, Ahu Sarbay; Sumer, Aziz; Begenik, Huseyin; Dulger, Ahmet Cumhur; Purisa, Sevim; Tuzun, Sefa
Background and objectives: To determine the serum levels of procarboxypeptidase A (pro-CPA) and carboxypeptidase A (CPA) in patients with acute and chronic pancreatitis and pancreatic cancer. Materials and methods: Serum samples obtained from 96 patients with acute pancreatitis, 101 patients with chronic pancreatitis, 98 patients with pancreatic cancer and 96 control groups were assayed for biochemical parameters and serum pro-CPA and CPA. Results: Serum pro-CPA and CPA levels were significantly higher in acute and in chronic pancreatic cancer patients compared to control group (p < 0.001). Pancreatic cancer patients had significantly higher serum pro-CPA and CPA levels when compared with acute and chronic pancreatitis cases (p < 0.001). Conclusion: These data prove for increased pro-CPA and CPA levels as a biomarker for the diagnosis of pancreatitis and pancreatic cancer.