Browsing by Author "Ucar, Ceylan Pinar"

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Effect of Pseudomonas Spp. and Funneliformis Mosseae on Bacterial Spot Disease and Growth Response of Pepper
(Springer, 2024) Cakar, Kamuran; Akkopru, Ahmet; Ucar, Ceylan Pinar
The interaction of plant-growth promoting rhizobacteria (PGPR) and Arbuscular mycorrhizal fungi (AMF), used in biological control, could determine the outcome of the biocontrol of plant disease. The efficacy of AMF species (Funneliformi mosseae [Fm]) and PGPRs (Pseudomonas spp. V31Y4 and P. fluorescens WCS365) on pepper plant bacterial spot disease caused by Xanthomonas euvesicatoria (Xe) and host plant growth parameters were investigated. Bacteria biocontrol agents were suppressed significantly the disease severity, however Fm alone was not effective. Combination of Fm and WCS365 was the most successful treatment for disease suppression with a rate of 15% compared with the control. There was no significant difference among treatments with Xe induced defoliation. However, the number of leaves in plants inoculated with Fm and Xe decreased significantly compared with control plants. Nevertheless, single application of Fm and PGPRs significantly increased plant biomass, height and chlorophyll content, and the bacterial strains were more successful. The growth response (GR) value in plants ranged from 22 to 28.6% as a single or combination treatment without the disease pressure. However, under the disease pressure, this value changed from 3.6 to 20.9%. In disease-free conditions, V31Y4 treatment reduced Fm colonization, while WCS365 increased colonization. Conversely, under disease pressure, WCS365 treatment had no effect on Fm colonization while V31Y4 treatment increased it compared with the control. There was no significant relationship between phosphorus content and GR with disease severity.Finally, Fm application increased susceptibility to Xe in pepper plants, but the suitable PGPR and AMF combination treatment was more successful than single applications for disease suppression caused by Xe in pepper plants.
The Effects of Endophytic Bacteria Along With Humic Acid and Biochar on Phytoremediation of Rapeseed (Brassica Napus L.)
(Springer, 2024) Canal, Sibel Boysan; Bozkurt, Mehmet Ali; Ucar, Ceylan Pinar
This study carries out the impact of endophytic bacteria on phytoremediation in rapeseed plants. Additionally, this study carries out the impact of endophytic bacteria in the presence of humic acid and biochar on phytoremediation. For this purpose, 50 mg kg(-1) Cd, 50 mg kg(-1) Pb, and 50 mg kg(-1) Cr were applied to the soil, and then (Humic acid) HA 1000 mg kg(-1) and 10% BC (Biochar) were applied and left for incubation. Two types of endotypic bacteria (EB1: Bacillus mojavensis, G106K1 EB2; Pantoea agglomerans, G129K1-1) were applied to these growing media of the three-leaf rapeseed plant and clean soil as a control. After the isolation was performed, the phytoremediation capabilities of the bacteria Bacillus mojavensis and Pantoea agglomerans were examined for the first time. Endophytic bacteria with HA and BC, resulted in an increase in the growth of rapeseed plants. In combination with HA and endophytic bacteria, values of (bioconcentration factor) BCF(shoot) and BCF(root) resulted in an increase for Cr and Cd. Furthermore, the application of endophytic bacteria with HA increased the (transfer factor) TF > 1 value for Cd. In this case, HA also increased the transfer of Cd from the root region to the shoot. With the applications of HA and BC with endophytic bacteria, enzyme activity decreased in the plant compared with a single endophytic bacteria treatment. We concluded that endophytic bacteria applied with biostimulants can be effective for the phytoextraction of hyperaccumulator plants in soils with low organic matter under heavy metal conditions.
Isolation and Characterization of Endophytic Bacteria From the Poaceae Family: Assessing Their Impact on Drought Stress and Plant Growth in Wheat
(Springer, 2025) Akkopru, Ahmet; Taskin, Bilgin; Arpali, Digdem; Ucar, Ceylan Pinar; Yardim, Dilek Ozcan; Ozturkci, Yusuf
Limited water resources and the increasing impact of global warming highlight the importance of endophytic bacteria (EB) given their unique tolerance to drought stress. In this study, we isolated EB from cultivated and wild Poaceae family members grown in arid or challenging environments in Lake Van Basin. We aimed to reveal plant growth promoting (PGP) characters in EB and to determine their potential to promote plant growth and reduce the effect of different drought levels in two different wheat cultivars (Tir (tolerant) and Bezostaja-1). 410 EB were isolated from 135 plants belonging to 19 taxa at different periods. In vitro tests revealed that 73.1% of the isolates can synthesize indole acetic acid (IAA), 34.4% can fix nitrogen, 37% can synthesize 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (ACCd), 37.6% can solubilize phosphorus, and 93.9% can synthesize siderophore. IAA production and phosphorous solubility were the most common characters, coexisting in 59% of EBs. ACCd production, phosphorus solubilization, and nitrogen fixation were the most common features with a triple association of 0.49%. Although isolates selected according to EB characters showed differing effects on coleoptile, root and shoot length, germination percentage, shoot and root weight, and relative water content (RWC) in the early development period (EDP) of Bezostaja-1 cultivar under drought stress, some isolates had negative effects. However, Pseudomonas asturiensis G58S1, Pantoea agglomerans G129K1-1, Chryseobacterium rhizosphaerae G129S2, Acinetobacter calcoaceticus G118K1T, and Stenotrophomonas sp. G109K2-K isolates selected according to PGP trials and EDP results showed improved plant biomass, chlorophyll content, membrane permeability, germination power, and RCW, and protective effects against drought.