Browsing by Author "Ulker, Hasan"

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Associations Between Cyp17 Gene Polymorphisms, Temperament and Maternal Behavior in Ewes, and Growth in Their Lambs
(Elsevier Science inc, 2021) Karaca, Serhat; Ser, Gazel; Ulker, Hasan; Yilmaz, Onur; Cakmakci, Cihan; Ata, Nezih; Saribey, Muammer
This study was conducted to determine temperament and maternal care classes, CYP17 gene polymor-phisms, and cortisol status of a group of Norduz ewes. Additionally, the relationships between these fac-tors, their effects on lambs survival and growth characteristics were investigated. A total of 72 ewes were subjected to arena and isolation box tests to determine their temperament classes. The distance the ewe retreated from the lamb during tagging was utilized to assess ewe maternal care classes. Ewes were genotyped for CYP17 628 SNP. Blood samples were collected between lambing and weaning to measure cortisol concentrations of ewes. The survival and growth data for the lambs of these ewes were recorded until weaning. Two temperament (low reactive and high reactive) and 2 maternal care classes (strong and moderate) were present. Two CYP 17 SNP 628 alleles (A and G) and 3 genotypes (AA, AG, and GG) were identified. For the distribution of CYP17 genotype frequencies within the population, the temperament or maternal care classes were not different (P > 0.05). The temperament of ewes did not affect mater-nal care in our flock. Positive partial correlations between cortisol and total cross, distance to stimulus, and sniffing were determined (P < 0.05). CYP17 genotype was an effective factor for some behavioral traits (i.e., total cross) and cortisol release (P < 0.001). Neither temperament (P = 0.636) nor maternal care classes (P = 0.985) differed with cortisol concentrations. CYP17 genotype, cortisol status, tempera-ment or maternal care class of ewes did not affect birth or weaning weight of their lambs (P > 0.05). Although there was no significant relationship among CYP17 genotype, maternal care, and lamb survival and growth, the effect of CYP17 genotype on some behavioral traits and cortisol release was found to be significant. Further studies with larger animal populations are required to elucidate the possible use of CYP17 SNP 628 genotypes as genetic markers for temperament classification. (c) 2021 Elsevier Inc. All rights reserved.
Cpg Motif-Based Adjuvant Enhances Immunogenicity of a Recombinant Lhrh Vaccine
(Soc Study Reproduction, 2007) Conforti, Valeria; de Avila, David; Wells, Kenny; Zanella, Ricardo; Cummings, Nada; Ulker, Hasan; Reeves, Jerry
Does the Length of Time Dried Distillers' Grain With Solubles Substitution for Soybean Meal Affect Physiological Indicators and Meat Quality in Finishing Lambs
(Wiley, 2021) Karaca, Serhat; Erdogan, Sibel; Guney, Mehtap; Cakmakci, Cihan; Saribey, Muammer; Kor, Askin; Ulker, Hasan
This study examined how inclusion of dried distillers' grains with solubles (DDGS) in finishing lamb diets for different periods affects some physiological indicators and meat quality. A total of 40 male lambs were divided into four groups according to feeding regimen during the 120-day finishing period as follows: C120: no DDGS included in diet for 120 days; D120: DDGS included in diet for 120 days; D75: no DDGS included in diet for 45 days + DDGS included in diet for 75 days; D45: no DDGS included in diet for 75 days + DDGS included in diet for 45 days. Dietary inclusion rate of DDGS was 27.5%. Fattening performance and rumen parameters were not affected by treatment. Feeding regimens had no significant effect on meat quality except the instrumental tenderness and juiciness score. DDGS fed lambs had higher level of total trans fatty acids and n-6/n-3 ratio in meat. 10t-C18:1, 11t-C18:1 and 9c,11t conjugated linoleic acid contents of adipose tissue were higher in all the DDGS groups compared with C120 lambs (p < .001). These results suggest that soybean meal can be replaced with corn DDGS in lamb diets for up to 120 days during the finishing period with no adverse effects on some physiological response feedlot performance and meat quality.
The Effectiveness of Recombinant Ol Fusion Protein (ovalbumin-Lhrh in Suppressing Reproductive Functions When Injected in Single-Dose Vaccination Protocols With Different Adjuvants
(Elsevier Science Bv, 2013) Karakus, Ferda; Yilmaz, Ayhan; Hakan, Bunyamin; Stormo, Keith; Ulker, Hasan
The objective of this study was to evaluate the effectiveness of recombinant LHRH fusion protein, Ovalbumin-LHRH-7 (OL), using a single-dose vaccination protocol in combination with different adjuvants in suppressing reproductive functions in buck kids. For this purpose, either a mixture of free OL antigen and encapsulated OL antigen, or encapsulated OL antigen was used. Thirty-nine native buck kids at 12 weeks of age were divided into control (n = 7) and treatment groups (n = 8 bucks/group). The four treatment groups were formed according to the different vaccine formulations: Group CpG received 0.5 mg free OL protein together with 1.0 mg of encapsulated protein with CpG adjuvant. Group mFCA received 0.5 mg free OL protein together with 1.0 mg of encapsulated protein with modified Freund's complete adjuvant. Group IS received 1.5 mg encapsulated OL protein with a mix of inulin and saponin adjuvants. Group ISmFCA received 1.5 mg encapsulated OL protein with a mix of inulin, saponin and modified Freund's complete adjuvants. Scrotal circumference in CpG and mFCA groups were significantly smaller than that of Control, IS and ISmFCA groups (P < 0.05). Numbers and percentage of bucks having spermatozoa in their ejaculate were significantly lower in CpG and mFCA groups (P < 0.05). OL immunization completely suppressed sperm production, except one buck, in CpG and mFCA groups (P < 0.05). These results imply that it is possible to use OL protein in a single injection protocol for the purpose of immunocastration. Further investigation with a larger number of animals should be carried out to determine the longevity of response to a single injection. (C) 2013 Elsevier B.V. All rights reserved.
The Effects of Immunological Castration Against Gnrh With Recombinant Ol Protein (ovalbumin-Lhrh on Carcass and Meat Quality Characteristics, Histological Appearance of Testes and Pituitary Gland in Kivircik Male Lambs
(Elsevier Sci Ltd, 2010) Gokdal, Ozdal; Atay, Okan; Ulker, Hasan; Kayaardi, Semra; Kanter, Mehmet; DeAvila, Michael David; Reeves, John Jerry
The aims of the study were to investigate the effects of immunization against GnRH using OL protein (Ovalbumin-LHRH-7) on feedlot performance, carcass, meat quality and some reproductive traits in Kivircik ram lambs. Ram lambs in the immunization (I, n = 7) group were immunized against GnRH using OL protein and boosted 2 weeks later. Control (C, n = 7) group was not treated. The animals were kept at pasture for 6 weeks after the first immunization, subjected to a 70 day fattening program, and then slaughtered. Growth performance, various carcass and meat quality characteristics were not affected from the immunization. GnRH immunization induced GnRH antibody production, suppressed testosterone production and testicular growth (P<0.01). Testicular structure was negatively affected from the immunization, but not pituitary. These results suggest that immunization against GnRH with OL could be an alternative castration technique in ram lambs without negatively affecting carcass and meat quality characteristics. (C) 2010 The American Meat Science Association. Published by Elsevier Ltd. All rights reserved.
Evaluation of One-Shot Vaccination Protocol for Suppressing Reproductive Functions in Rams Using Encapsulated Ovalbumin-Lhrh Protein
(Agricultural Research Communication Centre, 2018) Yilmaz, Ayhan; Karakus, Ferda; Hakan, Bunyamin; Stormo, Keith; Cengiz, Firat; Ulker, Hasan
The objective of study were to determine the effectiveness of Ovalbumin-LHRH-7 (OL) protein administered with cytosine guanine (CpG) adjuvant and Incomplete Freund's Adjuvant (IFA), and used one-shot immunization (single-dose vaccination) protocol in which booster dose included in microspheres in rams. Fifty ram lambs at about a year old were used. Treatment groups receiving Ovalbumin LHRH and control contained 10 animals. They were stratified according to age (weeks), live weight and scrotal circumference size, and were randomly assigned to five groups. Scrotal circumference, sexual activities and the numbers of rams having sperm in the ejaculate were affected from treatment (P<0.05) depending on the dose and vaccination protocol. However, immunization did not affect live weight changes in any treatment groups (P>0.05). Findings clearly demonstrate that the effects of OL immunization on reproductive traits in yearling rams were prominent when it was administered at higher dose and classical one primary and one booster immunization as free protein form. Also we observed that the effect of higher and single dose of OL protein in encapsulated form on reproductive traits had the partial suppressing. CpG adjuvant along with IFA was proved to be an effective adjuvant and could be suggested to be used and alternative to FCA in hormone immunization.
The Use of Encapsulated Ovalbumin-Lhrh (Ol) Protein in Single-Dose Vaccination Protocols for Lhrh Immunization
(Polska Akad Nauk, inst Genetyki I Hodowli Zwierzat, 2013) Ulker, Hasan; Stormo, Keith; deAvila, David; Reeves, J. Jerry
The objective of this study was to evaluate effectiveness of Ovalbumin-LHRH-7 (OL) protein when injected in crude, purified, free or encapsulated forms and using a single vaccination protocol along with CpG, inulin and saponin adjuvants. Fifty six C57BL/6 mice in seven groups (n=8) received various treatments and doses: Group 1 was control; Group 2 and 3 were injected twice with purified or crude OL protein, respectively, 4 wks apart. Group 4 and 5 were injected only once with purified or crude OL protein, respectively. Group 6 was injected only once with a mix of purified OL protein and encapsulated purified OL protein. Group 7 was injected only once with a mix of crude OL protein and encapsulated crude OL protein. There was an immunization effect observed on the I-125 LHRH % binding (P<0.05). Antibodies (Abs) against LHRH were identified on week 5 of immunization in groups 2, 3 and 4. Boosting at week 5 caused a significant increase in LHRH antibody (Ab) concentrations in groups 2 and 3. Numbers of pregnant animals and prengnancy rates were suppressed in all treatment groups at various degrees (P<0.05). Numbers of pups born were affected by immunization (P<0.05). Concluding, immunization with OL protein generated either biological or both immunological and biological effects in the most of treatment groups. The study confirmed the earlier findings that purified OL protein with CpG adjuvant is effective in inducing immune response and suppressing reproductive functions. However, the original idea that the non-capsulated antigen/adjuvant mix would work as primary injection, while encapsulated counterpart would mimic booster injections in a single vaccination protocol could not be confirmed in this study. Further studies to determine affecting factors for single-injection LHRH immunization are needed.