Browsing by Author "Ulker, Mehmet"

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Estimation of Nuclear Dna Content in Some Aegilops Species: Best Analyzed Using Flow Cytometry
(Mdpi, 2022) Najafi, Solmaz; Ulker, Mehmet; Oral, Erol; Tuncturk, Ruveyde; Tuncturk, Murat; Sayyed, R. Z.; Cseh, Andras
The genera Triticum and Aegilops have been considered as the main gene pool of wheat due to their features, such as tolerance of all types of abiotic and biotic stresses. This study was conducted to evaluate the cytogenetic analyses in 115 native and wild populations from eleven Aegilops species using their nuclear DNA quantification. Mean 2C nuclear DNA contents of different ploidy levels in the wild wheat of Turkey and Iran were measured using the flow cytometry technique. The obtained results showed that the mean nuclear DNA content in diploid species varied from 10.09 pg/2C (Ae. umbellulata) to 10.95 pg/2C (Ae. speltoides var. ligustica) in Turkey. In Iranian diploids, the mean nuclear DNA content varied from 10.20 pg/2C (Ae. taushii) to 11.56 pg/2C (Ae. speltoides var. ligustica). This index in the tetraploid species of Turkey varied from 18.09 pg/2C (Ae. cylindrica) to 21.65 pg/2C (Ae. triaristata), and in Iranian species, it was from 18.61 pg/2C (Ae. cylindrica) to 21.75 pg/2C (Ae. columnaris). On the other hand, in the hexaploid species of Turkey, this index varied from 31.59 pg/2C (Ae. crassa) to 31.81 pg/2C (Ae. cylindrica); in the Iranian species, it varied from 32.58 pg/2C (Ae. cylindrica) to 33.97 pg/2C (Ae. crassa). There was a significant difference in the DNA content of Turkey and Iran diploid as well as tetraploid species; however, in hexaploid species, the difference was not significant. It was concluded that the variation in intraspecific genome size was very low in diploid and tetraploid populations; this means that the low variation is not dependent on geographic and climatic parameters. On the other hand, the interspecific variation is significant at the diploid and tetraploid populations. It is generally very difficult to distinguish Aegilops species from each other in natural conditions; meanwhile, in this study, all species could be, easily, quickly and unambiguously, distinguished and separated using the FCM technique.
Identifying Amf-Rich Tir Wheat Rhizospheres To Foster Microbial Inoculants Useful in Sustainable Agriculture: Evidence From the Van Lake Basin
(Mdpi, 2025) Najafi, Solmaz; Ulker, Mehmet; Danesh, Younes Rezaee; Demir, Semra; Oral, Erol; Altuner, Fevzi; Pellegrini, Marika
Arbuscular mycorrhizal fungi (AMF) play a pivotal role in sustainable agriculture by enhancing nutrient efficiency and reducing the dependence on synthetic fertilizers. Developing these sustainable, effective products requires knowledge of the target plant and its associated microbial communities in the production landscape of interest. This study focused on AMF populations associated with Tir wheat in six main locations of T & uuml;rkiye's Van Lake Basin. The Er & ccedil;ek-& Ouml;zalp-Saray region exhibited the highest organic matter values. Higher available phosphorous contents were found for Erci & scedil;-Patnos and Muradiye. The Erci & scedil;-Patnos region exhibited the highest AMF density (120 spores/10 g soil) and frequency (75%), while the lowest AMF density (45 spores/10 g soil) was recorded in Muradiye. Sand contents correlated positively with spore number and mycorrhizal frequency and negatively with silt and clay. Based on these results, Erci & scedil;-Patnos was elected as the best location for the isolation of AMF spores suitable for the development of microbial-based tools for Tir wheat cultivation. These results are very important in the current context of climate change, which mandates the use of low-impact environmental strategies. Further research should explore the interactions of AMFs with other microorganisms to optimize their ecological benefits. However, the results of this study provide a valuable basis for future investigations of AMF-based products for use in sustainable Tir wheat cultivation.