Browsing by Author "Unal, Murat"

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Comparison of Hepcidin and Other Acute Phase Reactants in Patients Diagnosed With Pyelonephritis
(2022) Unal, Murat; Binici, İrfan
Üriner sistem enfeksiyonu, üriner sistemi oluşturan yapıların herhangi bir yerinde mikroorganizmanın yerleşmesi ile oluşan klinik tablodur. Tüm yaş gruplarında, toplumdan edinilmiş enfeksiyonlar arasında, üst solunum yolu enfeksiyonlarından sonra ikinci sırada yer alırken, hastane ve sağlık bakım merkezlerinde edinilmiş enfeksiyonlar arasında birinci sırada yer alır. Akut piyelonefrit, üst üriner sistemin, özellikle de renal parankim ve toplayıcı sistemin enfeksiyonu olarak tanımlanır. Hafif bir tablodan sepsise kadar değişebilen geniş bir yelpazede ortaya çıkabilir. Sık görülen klinik bulguları idrar yaparken yanma (dizüri), sık idrara çıkma (pollaküri), yan ağrısı, üşüme-titreme ve ateştir. Hepsidin son dönemlerde keşfedilmiş peptid yapıda bir hormondur. Hepsidinin demir metabolizmasındaki rolü netlik kazanmasına rağmen enflamasyondaki rolü belirsizliğini korumaktadır. Yapılan insan ve hayvan çalışmalarında hepsidin sentezinin enfeksiyon durumlarında arttığı gösterilmiştir. Piyelonefrit tedavi edilmediği takdirde sepsis gelişebilir ve kesin bir tanı kriteri yoktur. Tanı klinik bulgular ve fizik muayene ile konulur, laboratuvar ve görüntüleme yöntemleri ile desteklenir. Ancak bazı olgularda tanı koymak zor olabilir. Kesin bir tanısı olmayan piyelonefritte hepsidin, tanıya yardımcı erken bir marker olabilir. Böylelikle tanı daha erken konulabilir, mortalite ve morbidite oranları düşürülebilir ve artan maliyet oranları azaltılabilir. Materyal ve Metod: Prospektif olarak tasarlanan bu çalışma, Mart 2021-Mart 2022 tarihleri arasında Van Yüzüncü Yıl Üniversitesi Tıp Fakültesi Dursun Odabaş Tıp Merkezi Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji kliniğine başvuran 18 yaş üstü pyelonefrit tanısı alan 60 hasta ve benzer yaş ve cinsiyette herhangi bir sağlık problemi olmayan 40 erişkin birey kontrol grubu ile yapılmıştır. Hastalarda tedavi öncesi ve tedavi sonrası hepsidin, prokalsitonin, C-reaktive protein, hemogram ve sedimantasyon düzeylerine bakıldı. Kontrol grubundan hepsidin için serum örnekleri alındı Bulgular: Çalışmamıza dahil edilen piyelonefrit grubundaki hastaların tedavi öncesi ortanca hepsidin düzeyi 1393,43 (1131,76-2069,96) pg/ml, tedavi sonrası ortanca hepsidin düzeyi 1221,11 (931,78-2023,16) pg/ml ve sağlıklı kontrol grubundaki ortanca hepsidin düzeyi 1114,74 (774,35-1678,51) pg/ml olarak tespit edildi. Piyelonefrit hasta grubunun tedavi öncesi ile tedavi sonrası hepsidin düzeyleri arasında istatistiksel olarak anlamlı fark tespit edildi (p=0,014). Piyelonefrit hasta grubunun tedavi öncesi ile sağlıklı kontrol grubunun serum hepsidin düzeyleri arasında da istatistiksel olarak anlamlı fark bulundu (p=0,005) ancak piyelonefrit hasta grubunun tedavi sonrası ile sağlıklı kontrol grubu arasında istatistiksel olarak fark tespit edilmedi (p=0,23). Sonuç: Hepsidinin enfeksiyondaki rolü netlik kazanmamıştır. Toplumda önemli bir mortalite ve morbidite nedeni olan akut piyelonefrit tanısında kullanılacak spesifik bir belirtecin olmadığı göz önüne alındığında hepsidinin bu hastalarda tanıda yardımcı bir biyobelirteç olarak kullanılabilme potansiyelinin göz önünde bulundurulması gerektiği kanaatindeyiz.
Essential Oil Compositions and Antimicrobial Activity of Salvia Species
(Taylor & Francis Ltd, 2007) Altun, Mehmet; Unal, Murat; Kocagoz, Tanil; Goren, Ahmet C.
The essential oil compositions of aerial parts of Salvia macroclamys, S. verticillata ssp. amasiaca, S. virgata, S. multicaulis, S. firigida, S. microstegia and S. kronenburgii were analyzed by gas chromatography-mass spectrometry (GC-MS). Their main constituents were found to be alpha-pinene, beta-pinene, 1,8-cineole, thymol, and caryophyllene oxide. Essential oil of S. multicaulis, S. kronenburgii, S. verticillata and the pure compounds alpha-pinene, beta-pinene, 1,8-cineole, linalool oxide and caryophyllene oxide were tested against E. coli, S aureus, K. pneumonia, and the yeast C.albicans. Key words: Salvia, essential. oil, antimicrobial activity, GC-MS, alpha-pinene, beta-pinene,
Fruit and Seed Macro- and Micromorphologies of the Genus Matthiola (Brassicaceae) in Turkey and Their Taxonomic Value
(Tubitak Scientific & Technological Research Council Turkey, 2019) Kaya, Ayla; Unal, Murat; Sefali, Abdurrahman; Martin, Esra
Macro- and micromorphological features of fruit and seeds belonging to 12 taxa genera of Matthiola W.T. Aiton were studied via stereomicroscope and scanning electron microscope. In macromorphological studies, the following variables were investigated: the shape, size, and color of fruit and seeds; the length of fruiting pedicel; the structure of the median vein; stigma (horns); and trichome properties and density in fruit. In micromorphological studies, the presence or absence of seed wings, seed coat pattern in disc and wing, and epidermal cell shape in disc and wing were determined. According to our findings, all characters of fruit and seeds are extremely variable and can be used as criteria to distinguish species of the genus.
Fruit and Seed Morphology of Six Species Previously Placed in Malcolmia (Brassicaceae) in Turkey and Their Taxonomic Value
(Tubitak Scientific & Technological Research Council Turkey, 2011) Kaya, Ayla; Unal, Murat; Ozgokce, Fevzi; Dogan, Bekir; Martin, Esra
The fruit and seed morphologies of 6 species previously placed in the genus Malcolmia W.T.Aiton were examined with stereomicroscopy and scanning electron microscopy (SEM) in order to determine the significance of fruit and seed features as taxonomic characters. This study presents macro- and micromorphological characters, including fruit and seed shape, size, colour, fruiting pedicel size, fruit hair and density, seed coat pattern, epidermal cell shape, and anticlinal and periclinal cell walls. The results showed that the morphological characteristics of the fruit and seed could be used as criteria to distinguish genera and tribes. The investigated features of specimens indicated that Malcolmia chia (L.) DC. and M.flexuosa (Sikh. & Sm.) Sibth. & Sm. should remain in the genus that belongs to the tribe Anastaticeae. Malcolmia crenulata (DC.) Boiss. and M. exacoides (DC.) Spreng. belong to the genus Zuvanda (Dvorak) Askerova of the tribe Conringieae. Finally, Malcolmia africana (L.) W.T.Aiton and M. intermedia C.A.Mey. belong to the genus Strigosella Boiss. of the tribe Euclidieae. A key is provided for the identification of the investigated species based on fruit and seed characters.
Fruit, Seed and Pollen Morphology of Chorispora Dc. Species (Brassicaceae) of Turkey
(Bangladesh Botanical Soc, 2018) Satil, Fatih; Kaya, Ayla; Unal, Murat
Detailed description of fruit, seed and pollen macro- and micromorphological characters of Turkish Chorispora species are provided with illustrations. Typical fruits are linear, straight or strongly curved upward. Nonglandular and glandular trichomes are present or absent. Seeds varied in shape from oblong, oblong-broadly elliptic to subglobose and winged at the apex and base or not. The pollen grains are tricolpate and the basic shape of the pollen grains in species studied is perprolate. The surface sculpturing type is reticulate. Among the studied characters, fruit, seed size and colour, seed shape, fruit trichome structure and pollen size were of taxonomic importance and useful in separating taxa.
Hematologic Inflammation Indices for Differentiating Between Brucella, Pyogenic, and Tuberculous Spondylodiscitis
(Mdpi, 2024) Baran, Ali Irfan; Binici, Irfan; Arslan, Yusuf; Karaduman, Zekiye Hakseven; Ilter, Server; Tarcan, Tayyar; Unal, Murat
Infectious spondylodiscitis is a life-threatening disease and has some challenges in terms of diagnostic, differentiative, and therapeutic processes. Therefore, rapid and effective management of infectious spondylodiscitis is necessary. Hematological inflammation indices (HIIs) such as the neutrophil/lymphocyte ratio and aggregate index of systemic inflammation are derived from blood cells and used as diagnostic, prognostic, predictive, and treatment monitoring indicators. This study aimed to evaluate HIIs for discriminating between infectious spondylodiscitis pathogens. This retrospective comparative study included 116 patients with infectious spondylodiscitis. According to the responsible infectious pathogens, three types of infectious spondylodiscitis were defined: Brucella (n = 51), pyogenic (n = 43), and tuberculous (n = 22). The HIIs were derived from baseline complete blood counts. The three types of infectious spondylodiscitis were statistically compared for the HII scores. We found that the Brucella group had significantly lower HII scores than the pyogenic group (p < 0.05). Also, the Brucella group had significantly lower HII scores than the tuberculous group (p < 0.05). However, no significant differences were found between the pyogenic and tuberculous groups regarding HIIs (p > 0.05). In conclusion, the HIIs may be considered in the differentiation between Brucella spondylodiscitis and other types of infectious spondylodiscitis.
Karyotype Analyses of the Genus Matthiola (Brassicaceae) in Turkey
(Univ Tokyo Cytologia, 2016) Martin, Esra; Unal, Murat; Dogan, Bekir; Altinordu, Fahim; Sefali, Abdurrahman; Kaya, Ayla
In the present paper, karyotype analyses are reported for 12 taxa of Matthiola from Turkey. Matthiola trojana Dirmenci, Satil & Ttimen, M odoratissima (Pall.) R.Br., M anchonifolia Hub.-Mor., M montana Boiss., M fruticulosa (L.) Maire ssp. fruticulosa and M ovatifolia (Boiss.) Boiss. counted 2n=12 chromosomes, and M incana (L.) R.Br., M longipetala (Vent.) DC. ssp. bicornis (Sibth. et Smith) P. W. Ball, M longipetala (Vent.) DC. ssp. longipetala, M longipetala (Vent.) DC. ssp. pumilio (Sibth. & Smith) P. W. Ball, M sinuata (L.) R.Br., and M tricuspidata (L.) R.Br. showed 2n=14 chromosomes. The idiograms and karyomorphometric data obtained by using Image Analysis System (Bs200Pro). The chromosome number of M montana was determined for the first time. Also, karyotype asymmetry index such as M-CA, CVCL and CVCL were measured for the reconstruction of karyological relationships.
Leptomeningeal Carcinomatosis Due To Gastric Adenocarcinoma Mixed With Meningitis
(Cukurova Univ, Fac Medicine, 2019) Sunnetcioglu, Mahmut; Celik, Mehmet; Unal, Murat; Tarcan, Tayyar; Erten, Remzi; Baran, Ali Irfan
A New Record for Turkey: Centaurea Aziziana Rech.f. (Asteraceae)
(Tubitak Scientific & Technological Research Council Turkey, 2009) Armagan, Metin; Unal, Murat
Centaurea aziziana Rech.f. (Asteraceae) is recorded for the first time (B9 Van province) for the flora of Turkey. The diagnostic characters of C. aziziana are described and its habitat and conservation are discussed.
Phylogenetic Relationships Between Malcolmia, Strigosella, Zuvanda, and Some Closely Related Genera (Brassicaceae) From Turkey Revealed by Inter-Simple Sequence Repeat Amplification
(Tubitak Scientific & Technological Research Council Turkey, 2011) Dogan, Bekir; Unal, Murat; Ozgokce, Fevzi; Martin, Esra; Kaya, Ayla
The genus Malcolmia W.T.Aiton is taxonomically problematic, and some of its species have recently been transferred to the genera Strigosella Boiss. and Zuvanda Dvorak. Three species of this genus are native to Turkey. The revision studies based on molecular data, which were collected in the past few years, display the phylogenetic relations and the systematic positions of the taxa more reliably and apparently. Thus taxonomic problems of species are resolved through DNA-based molecular analyses, which are not affected by environmental factors compared to phenotypic studies. In the present study, the amplifications of the DNA fragments were carried out using ISSR primers, and the phylogenetic relationship among the taxa was revealed through a dendrogram produced as the outcome of the NTSYSpc 2.1 software. The infrageneric and intergeneric phylogenetic relationships among Malcolmia and other related genera are determined. A very close relationship was determined between Malcolmia chin and M. flexuosa. Strigosella and Zuvanda species were determined to be phylogenetically different from these 2 species. The phylogenetic relationships among the Malcolmia, Strigosella, Zuvanda, Leptaleum DC., Neotorularia Hedge & J.Leonard, and Sisymbrium L. taxa were investigated. The phylogenetic separation of Malcolmia, Strigosella, Zuvanda Leptaleum, Neotorularia, and Sisymbrium genera and their specimens constituted separate clades on the dendrogram.
Phytochemical Investigation of Leontice Leontopetalum L. Subsp Ewersmannii With Antioxidant and Anticholinesterase Activities
(Acg Publications, 2011) Kolak, Ufuk; Hacibekiroglu, Isil; Boga, Mehmet; Ozgokce, Fevzi; Unal, Murat; Choudhary, M. Iqbal; Ulubelen, Ayhan
Two known quinolizidine alkaloids, lupanine and leontiformidine, were isolated from the tubers of L. leontopetalum subsp. ewersmannii. Lupanine having the highest inhibition of lipid peroxidation at 100 mu g/mL among the tested samples indicated almost the same ABTS cation radical scavenging activity with BHT, alpha-tocopherol and (+)-catechin at the same concentration. Lupanine and the alkaloidal extract showed almost the same butyrylcholinesterase inhibitory activity with galantamine at 200 mu g/mL.
Pokeweed (Phytolacca Americana L.) Antiviral Protein Inhibits Zucchini Yellow Mosaic Virus Infection in a Dose-Dependent Manner in Squash Plants
(Tubitak Scientific & Technological Research Council Turkey, 2017) Sipahioglu, Hikmet Murat; Kaya, Ilhan; Usta, Mustafa; Unal, Murat; Ozcan, Dilek; Ozer, Meryem; Pallas, Vicente
Pokeweed antiviral protein (PAP) of Phytolacca americana L. (pokeweed) is a single-chain ribosome-inactivating protein (RIP) characterized by its ability to depurinate plant ribosomes. Here, we isolated, cloned, and expressed the ribosome inactivating protein (RIP) gene, designated as pokeweed antiviral protein type 1 (PAP I), from the summer leaves of pokeweed collected from the Black Sea region (Turkey). Our findings presented here provide direct evidence that exogenous application of PAP I causes concentration-dependent inhibition of Zucchini yellow mosaic virus (ZYMV) infection on squash plants. Squash plants were exposed to PAP I protein with and without DMSO for four consecutive days. Regular spraying of approximately 30 kDa recombinant PAP I at 2 mu g mL(-1) concentration prevented treated plants from mechanical virus infection. PAP I showed antiviral activity in 9 plants out of 15 inoculated plants. Remarkably, simultaneous application of PAP, DMSO, and ZYMV did not prevent virus infection, suggesting that PAP did not have any effect on viral RNA. In the absence of ZYMV the purified peptide was not cytotoxic for squash plants, although a reduction of plant size, possibly caused by host ribosome depurination, was observed.
Pollen Morphology of Six Species Previously Placed in Malcolmia (Brassicaceae) in Turkey
(Bangladesh Botanical Soc, 2017) Kaya, Ayla; Unal, Murat; Ozgokce, Fevzi; Dogan, Bekir; Martin, Esra
The genus Malcolmia R. Br. (Brassicaceae) is taxonomically problematic and some of its species have recently been transferred to the genera Strigosella Boiss. and Zuvanda Dvorak. In this study, pollen morphology of some species of Malcolmia, Strigosella and Zuvanda, previously placed in illalcolmia genus, were examined under scanning electron microscope (SEM), in order to determine the significance of pollen features as taxonomic characters. The results reveal rather uniform morphological features, however fine details are characteristic to distinguishing pollen grains in the species of the genus. The pollen grains in three genera are tricolpate and the surface sculpturing type is reticulate. The basic shape of the pollen grains in species studied is euprolate, subprolate-euprolate and euprolate-perprolate. While pollen grains of S. africana is the smallest in all examined species, M. chic is the.biggest. However, three genera can be easily separated by the length of the polar and equatorial axes themselves.
Study of Phylogenetic Relationship of Turkish Species of Matthiola (Brassicaceae) Based on Issr Amplification
(Tubitak Scientific & Technological Research Council Turkey, 2016) Dogan, Bekir; Celik, Mustafa; Unal, Murat; Sefali, Abdurrahman; Martin, Esra; Kaya, Ayla
Matthiola W.T. Aiton is a taxonomically complex genus in which there are many problems, mostly with Matthiola longipetala and M. odoratissima. Matthiola species native to Turkey were collected from various locations in Anatolia, and their DNA was isolated. Revision studies performed on the basis of molecular data obtained from studies conducted in recent years have made the phylogenetic relationships and systematic positions of the taxa more apparent and reliable. Consequently, the remaining taxonomic problems among the species have been resolved through the use of DNA-based molecular analysis methods, which, unlike phenotype studies, are not affected by environmental factors. The inter-simple sequence repeat (ISSR) fingerprinting method was used in the study because its properties were considered to be more reliable and consistent than those of the randomly amplified polymorphic DNA method. DNA fragments were amplified through the use of ISSR primers. The phylogenetic relationships among the taxa were represented on a dendrogram constructed through means of NTSYSpc 2.02 software. The infrageneric and intergeneric phylogenetic relationships between Matthiola and other related genera were also characterized. It was determined that the taxa Matthiola odoratissima and M. ovatifolia are separate but closely related. Moreover, it was observed that the Matthiola longipetala complex forms a separate group within the genera. Clearly, the genera Matthiola, Sterigmostemum, Strigosella, Malcolmia, and Chorispora are phylogenetically differentiated on the dendrogram.
Traditional Medicinal Plants of Agri Province, Turkey
(Elsevier Ireland Ltd, 2018) Dalar, Abdullah; Mukemre, Muzaffer; Unal, Murat; Ozgokce, Fevzi
Ethnopharmacological relevance: This study serves as the first investigation of the knowledge of traditional medicinal plants used in Agri Province. The mountainous and strongly fragmented area, diversity of local flora, limited access to modern medicines because of the people's seminomadic lifestyle, and harsh climatic conditions have prompted the locals to benefit from natural resources, principally plants. Aim of the study: Although the use of medicinal plant is common in the area, documentation and inventory analysis are not available on the plant taxa used in traditional medicine in Agri Province. This study focused on the answer of the following question: What are the most medicinally valuable plant taxa in the province? Therefore, this study aimed to (i) document medicinal plant taxa used in Agri Province; (ii) determine the endemic plant species and their risk categories; (iii) determine the most widely used plant taxa for specific ailments to further contribute to drug discovery; (iv) determine the local names, plant part(s) used, preparation, and utilization methods of these local plant medicines to assist with pharmaceutical studies; and (v) reveal the origin(s) of traditional medicinal knowledge of Agri Province. Materials and methods: Ethnopharmacological field studies were conducted between 2014 and 2016 in 638 locations within Agri Province. Plant samples used in folk medicine were determined through face-to-face interviews with 120 local healers. Traditional medicinal plant samples were collected from wild areas with the help of the local healers and transported to Van Pharmaceutical Herbarium (Van, Turkey) for botanical identification. The statistical analyses of data were conducted using the informant consensus factor (ICF) method. Results: In this study, 118 medicinal taxa belonging to 35 families of plants were determined to be used in Agri Province. Ninety-three different local names were recorded, which mainly described the plant's morphological characteristics. Data analysis (ICF) revealed that the traditional medicinal plants used in Agri Province might offer promising information for new pharmaceuticals. Conclusions: The local population in the study area has a preserved and comprehensive knowledge of traditional medicine because of its limited access to modern medicine, seminomadic lifestyle, isolated geographical position, traditional taste habits, climatic conditions, diversity of local flora, and mountainous and rugged geography. The medicinal plants presented in this study might provide valuable leads for the identification of bioactive compounds for pharmaceutical use and represent a vast and underutilized resource for the development of newer and more efficient pharmacological treatments.