Browsing by Author "Unver, Ali"

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Comparison of Blood Βhba Measurement Devices for Diagnosis of Subclinical Pregnancy Toxaemia in Sheep: a Field Study
(Wiley, 2024) Turgut, Ali Osman; Koca, Davut; Unver, Ali
Pregnancy toxaemia is a serious disease that occurs during the last trimester of pregnancy in sheep. Yet, in most cases, the disease may have a subclinical course. This study was aimed at comparing blood beta HBA measurement devices for diagnosis of subclinical pregnancy toxaemia in late pregnant sheep. In the study, a total of 50 blood samples were collected from Romanov (n = 30) and cross-bred Hamdani (n = 20) sheep. Blood beta HBA levels were measured using TaiDoc TD-4235 and CentriVet beta HBA hand-held meter. Randox beta HBA (Ranbut) assay was used as a reference laboratory method to compare hand-held meters. beta HBA value of 0.8 mmol/L was set as the cut-off value for diagnosis of subclinical pregnancy toxaemia. Statistical analyses were carried out using Minitab 21 and Jamovi software. In the study, the correlation of Randox-TaiDoc TD-4235 and Randox-CentriVet was .822 (p < .001) and .728 (p < .001), respectively. Based on the Ranbut assay, nine ewes out of 50 were diagnosed with subclinical pregnancy toxaemia. Specificity (detection of healthy ewes) and sensitivity (detection of ewes with subclinical pregnancy toxaemia) for TaiDoc TD-4235 and CentriVet hand-held meters were 100%, 77.8%, and 100%, 66.7%, respectively. In the receiver operating characteristic (ROC) analysis, areas under the ROC curve (AUC) were 0.976 and 0.920 for TaiDoc and CentriVet, respectively. Bland-Altman analysis revealed a bias of 0.092 mmol/L for TaiDoc and a bias of 0.132 mmol/L for CentriVet. TaiDoc hand-held meter shows a better correlation with the Randox Ranbut assay and greater sensitivity compared to the CentriVet hand-held meter. In conclusion, both TaiDoc and CentriVet hand-held meters can be securely used in the diagnosis of subclinical pregnancy toxaemia in sheep. For these reasons, subclinical pregnancy toxaemia and these devices will be evaluated within the scope of herd management programme in the sheep industry. It should also be taken into account that these conditions will affect the future fertility of the mother and offspring.
Comparison of Serum Biochemical Parameters in Ketotic and Healthy Siirt Colored Mohair Goats During Early Lactation to Identify Potential Biomarkers of Lactation Ketosis
(Univ Zulia, Facultad Ciencias Veterinarias, 2025) Irmak, Mehmet; Turgut, Ali Osman; Eroglu, Mehmet; Irak, Kivanc; Tufan, Tuncay; Koca, Davut; Unver, Ali
This study aimed to compare serum biochemical parameters between ketotic and healthy Siirt colored mohair goats during early lactation, to diagnose subclinical lactation ketosis and identify potential biomarkers. A total of 77 female goats, aged 2 to 5 years and within 30 days postpartum, were evaluated under similar management conditions. The animals were classified into two groups based on serum beta-hydroxybutyric acid levels: subclinical lactational ketosis (n = 37) and health controls (n = 40). beta-hydroxybutyric acid levels were significantly elevated in the ketosis group (0.891 +/- 0.0141 mmolL-1) compared to the control group (0.595 +/- 0.0159 mmolL-1, P<0.001), confirming its diagnostic value. Other serum parameters did not show significant differences between the groups (P>0.05). On the other hand, effect size analysis revealed that glucose level decreased in does with subclinical lactational ketosis while cholesterol increased (large effect size). The study concluded that most liver and metabolic indicators remained within normal ranges, while glucose and cholesterol levels changed due to subclinical lactational ketosis. These data indicate that feeding based on pasture under extensive conditions may be insufficient to meet the energy requirements of does in lactation and emphasize the importance of early biochemical screening during lactation for effective management of metabolic disorders during early lactation stage in goats. Therefore, it is recommended the further studies to expand the knowledge of the effect of lactational ketosis on metabolic changes in goats.
The Negative Effects of Subclinical Pregnancy Toxaemia on Fetal Skeletal Muscle Development and Evaluation of the Protective Effects of Dietary L-Carnitine Supplementation in Sheep
(Wiley, 2025) Turgut, Ali Osman; Kandil, Banu; Bilen, Ebru Karakaya; Koca, Davut; Unver, Ali; Isbilir, Fatma; Wehrend, Axel
This study evaluated the effects of subclinical pregnancy toxaemia (SPT) on fetal skeletal muscle development and assessed the potential protective role of L-carnitine supplementation during gestation. A total of 18 crossbred Hamdani ewes underwent oestrous synchronisation, natural mating and pregnancy confirmation via ultrasonography on day 45 post-mating. The ewes were managed according to NRC (2007) dietary guidelines until day 100 of gestation, after which they were assigned to three experimental groups: subclinical PT group (group 1; G1, n = 6), treatment group (subclinical PT + L-carnitine, group 2; G2, n = 6) and control group (group 3; G3, n = 6). Blood beta-hydroxybutyrate (beta HBA) concentrations were measured on day 100 and 138 of the gestation. Then, all ewes were slaughtered for fetal muscle sampling from the Musculus Longissimus Dorsi (MLD) and Vastus Lateralis (VL). Results indicated a significant reduction in muscle fibre number and fibre diameter in both MLD and VL in the SPT group (G1) compared to the control (G3) (p < 0.05). No significant differences were observed between G1 and G2 or between G2 and G3 for these parameters (p > 0.05). On the other hand, large effect sizes for group and pairwise comparisons imply that SPT may negatively affect prenatal muscle development and L-carnitine supports muscle development during the prepartum period. These findings highlight the negative effects of SPT and protective effects of L-carnitine supplementation on fetal skeletal muscle development in ewes with SPT. The observed deficits may negatively impact postnatal growth, survival rates and meat quality. Further investigations are warranted to optimise maternal nutrition strategies and evaluate therapeutic interventions aimed at mitigating the adverse impacts of SPT on fetal muscle development in ruminants. Furthermore, L-carnitine supplementation may be a useful in compensating for the negative effects of SPT.